pycom04g18080

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000194
Physical Location & Seq
Reverse (-)
20249014 .. 20249418
405 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18080.1

Sequence Viewer

Length: 405 bp
ATGGTGCGATGTTGGAAATTCCGATTAGTGACCCGAGACAGGTTGGTGTGGCCATTCGCAAAAAAAGGAATTTTTTCTGTCAAATCGAGATATCATTGGGCTGTTTCAAGGAGACCAACTCGTGGAAGCCCCAGCCCATCATCCTCTACTTCTATTCCCAGCTCTCTTTGGAAAGTAATCTGGAACTTGCAAACAACACCAAAAATTTGGTGTTTCATGTGGAAATCCCTTCACTCAGCCATTGCAACCATGGAGAATTTATTCAAGAAGAGATCGGCACAATCACCTGTATGCCTATTATGTAATATTCACGAGGAGTCGGTCGAGCACCTGTTCATCTTATGTCCTTGGGTCAAATTGGTTTGGTTTGGCAGTGCTCTTAATTTGAAAATCAACCAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

135

Amino Acids

15.69

Weight (kDa)

10.31

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 25 - 122 3e-16 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 4 cut(s) 17, 69, 204, 256
AfiI CCNNNNNNNGG 2 cut(s) 39, 122
AgsI TTSAA 3 cut(s) 108, 265, 388
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 1 cut(s) 162
AluI AGCT 1 cut(s) 162
Alw21I GWGCWC 2 cut(s) 330, 379
Alw26I GTCTC 2 cut(s) 30, 106
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 1 cut(s) 50
ApoI RAATTY 4 cut(s) 17, 69, 204, 256
Asp700I GAANNNNTTC 2 cut(s) 73, 260
AsuHPI GGTGA 1 cut(s) 276
AvaI CYCGRG 1 cut(s) 33
BalI TGGCCA 1 cut(s) 52
BauI CACGAG 2 cut(s) 120, 311
Bbv12I GWGCWC 2 cut(s) 330, 379
BccI CCATC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 398
BcoDI GTCTC 2 cut(s) 30, 106
Bme1390I CCNGG 1 cut(s) 398
BmeT110I CYCGRG 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 398
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 249, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 122
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 2 cut(s) 249, 347
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 39, 122
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 329
BseYI CCCAGC 2 cut(s) 131, 158
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 2 cut(s) 330, 379
BsiHKCI CYCGRG 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 39, 122
BsmAI GTCTC 2 cut(s) 30, 106
BsnI GGCC 1 cut(s) 52
Bso31I GGTCTC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 330, 379
Bsp143I GATC 1 cut(s) 272
Bsp19I CCATGG 1 cut(s) 249
BspANI GGCC 1 cut(s) 52
BspCNI CTCAG 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 249, 347
BssMI GATC 1 cut(s) 272
BssSI CACGAG 2 cut(s) 120, 311
BssT1I CCWWGG 2 cut(s) 249, 347
Bst2BI CACGAG 2 cut(s) 120, 311
Bst2UI CCWGG 1 cut(s) 398
Bst6I CTCTTC 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 1 cut(s) 275
BstMAI GTCTC 2 cut(s) 30, 106
BstMBI GATC 1 cut(s) 272
BstMCI CGRYCG 1 cut(s) 324
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 396
BstXI CCANNNNNNTGG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 52
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 22
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 379
CviAII CATG 2 cut(s) 217, 250
CviJI RGCY 6 cut(s) 52, 101, 129, 135, 162, 239
CviKI_1 RGCY 6 cut(s) 52, 101, 129, 135, 162, 239
DdeI CTNAG 1 cut(s) 235
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 50
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 249, 347
Eco31I GGTCTC 1 cut(s) 106
Eco32I GATATC 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 396
EcoRV GATATC 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 249, 347
ErhI CCWWGG 2 cut(s) 249, 347
FaeI CATG 2 cut(s) 220, 253
FaiI YATR 5 cut(s) 218, 251, 292, 301, 343
FatI CATG 2 cut(s) 216, 249
FokI GGATG 1 cut(s) 127
GsaI CCCAGC 2 cut(s) 135, 162
HaeIII GGCC 1 cut(s) 52
Hin1II CATG 2 cut(s) 220, 253
HinfI GANTC 1 cut(s) 317
HphI GGTGA 1 cut(s) 276
Hpy188I TCNGA 1 cut(s) 23
Hpy188III TCNNGA 4 cut(s) 87, 181, 265, 311
HpyAV CCTTC 1 cut(s) 239
HpyCH4V TGCA 2 cut(s) 190, 245
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 2 cut(s) 220, 253
Kzo9I GATC 1 cut(s) 272
LpnPI CCDG 7 cut(s) 25, 145, 166, 172, 300, 344, 383
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 1 cut(s) 280
MhlI GDGCHC 2 cut(s) 330, 379
MlsI TGGCCA 1 cut(s) 52
MluCI AATT 6 cut(s) 17, 69, 204, 256, 356, 382
MluNI TGGCCA 1 cut(s) 52
MlyI GAGTC 1 cut(s) 326
MnlI CCTC 2 cut(s) 154, 307
Mox20I TGGCCA 1 cut(s) 52
MroXI GAANNNNTTC 2 cut(s) 73, 260
MscI TGGCCA 1 cut(s) 52
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 289
Msp20I TGGCCA 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
NcoI CCATGG 1 cut(s) 249
NdeII GATC 1 cut(s) 272
NlaIII CATG 2 cut(s) 220, 253
NmuCI GTSAC 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 73, 260
PflMI CCANNNNNTGG 1 cut(s) 122
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 396
PspFI CCCAGC 2 cut(s) 131, 158
PspGI CCWGG 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 289
SaqAI TTAA 1 cut(s) 381
Sau3AI GATC 1 cut(s) 272
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 2 cut(s) 330, 379
SetI ASST 4 cut(s) 44, 164, 289, 333
SmiMI CAYNNNNRTG 1 cut(s) 289
Sse9I AATT 6 cut(s) 17, 69, 204, 256, 356, 382
SspI AATATT 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 2 cut(s) 249, 347
TaqI TCGA 2 cut(s) 86, 324
TaqII GACCGA 1 cut(s) 310
TasI AATT 6 cut(s) 17, 69, 204, 256, 356, 382
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 205, 325
TspRI CASTG 1 cut(s) 379
Van91I CCANNNNNTGG 1 cut(s) 122
XapI RAATTY 4 cut(s) 17, 69, 204, 256
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XmnI GAANNNNTTC 2 cut(s) 73, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.