Rmu_sc0005285.1_g000008

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005285.1
OG0000194
Physical Location & Seq
Reverse (-)
32458 .. 35731
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005285.1_g000008.1.cds

Sequence Viewer

Length: 573 bp
atgcctacaaggacccaagctggggttcatcgatggaaacctgcaaatatactcaagcttaatgtggatggtgcgtttgcatctcagttttcacatggaggaacaggtggcgttctaagaagttccactggcagctttgttgctgccttcatgaagcctgtgcaccatgcagtcattgaaactgattgtctcttggcagttcaagacttggcttggaccgatgtgaatctgtcggaacttaaaagccccagaaacctctgcagtctttccagtatgcagctgaccatagaattcggtggaggattagagcttctttgtaactctgtcaagattcataatgtcaatgtagaactgcaaaaggcagcagaaaagttaaccatgaaagatttactggcttggattcgtactaatttgatcaaggagaggcctgaaatgtttatgaaaggagattctgtgagacctggtgttctagcccttgtgaatgattgtgattgggagctgactggtcaacttgatacaacactagaggagaaggatgtggttgttttcatttcaaccttgcacggcggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Urm1

Protein Analysis

190

Amino Acids

20.8

Weight (kDa)

6.06

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AciI CCGC 1 cut(s) 567
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 2 cut(s) 21, 22
AgsI TTSAA 3 cut(s) 179, 203, 555
AjnI CCWGG 1 cut(s) 460
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 6 cut(s) 20, 58, 135, 280, 310, 499
AluI AGCT 6 cut(s) 20, 58, 135, 280, 310, 499
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 194, 451
Alw44I GTGCAC 1 cut(s) 161
AoxI GGCC 1 cut(s) 425
ApaLI GTGCAC 1 cut(s) 161
ApeKI GCWGC 4 cut(s) 132, 143, 277, 362
ApoI RAATTY 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 12, 216
AvaII GGWCC 2 cut(s) 12, 216
BaeGI GKGCMC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 4 cut(s) 130, 144, 289, 374
BccI CCATC 2 cut(s) 27, 62
BciT130I CCWGG 1 cut(s) 462
BclI TGATCA 1 cut(s) 414
BcoDI GTCTC 2 cut(s) 194, 451
BfaI CTAG 2 cut(s) 470, 524
BfmI CTRYAG 1 cut(s) 259
BfuAI ACCTGC 1 cut(s) 49
BisI GCNGC 4 cut(s) 133, 144, 278, 363
BlsI GCNGC 4 cut(s) 134, 145, 279, 364
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 2 cut(s) 12, 216
BmgT120I GGNCC 2 cut(s) 12, 216
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 22
Bse1I ACTGG 4 cut(s) 133, 270, 396, 508
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 462
BseCI ATCGAT 1 cut(s) 31
BseGI GGATG 2 cut(s) 73, 541
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 21, 22
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 4 cut(s) 133, 270, 396, 508
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 165
BseXI GCAGC 4 cut(s) 130, 144, 289, 374
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 1 cut(s) 427
BshVI ATCGAT 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 165
BslI CCNNNNNNNGG 2 cut(s) 21, 22
BsmAI GTCTC 2 cut(s) 194, 451
BsnI GGCC 1 cut(s) 427
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 1 cut(s) 414
BspACI CCGC 1 cut(s) 567
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 97
BspDI ATCGAT 1 cut(s) 31
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 14
BspMAI CTGCAG 1 cut(s) 263
BspMI ACCTGC 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 451
BsrI ACTGG 4 cut(s) 133, 270, 396, 508
BssMI GATC 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 84, 116
BstF5I GGATG 2 cut(s) 73, 541
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 2 cut(s) 194, 451
BstMBI GATC 1 cut(s) 414
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 1 cut(s) 165
BstV1I GCAGC 4 cut(s) 130, 144, 289, 374
Bsu15I ATCGAT 1 cut(s) 31
BsuRI GGCC 1 cut(s) 427
BsuTUI ATCGAT 1 cut(s) 31
BtsCI GGATG 2 cut(s) 73, 541
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 12, 216
ClaI ATCGAT 1 cut(s) 31
CsiI ACCWGGT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 405
CviAII CATG 4 cut(s) 95, 151, 167, 379
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 2 cut(s) 84, 116
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 12, 216
EcoO109I RGGNCCY 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 460
FaeI CATG 4 cut(s) 98, 154, 170, 382
FaiI YATR 9 cut(s) 50, 96, 152, 168, 275, 287, 336, 380, 440
FatI CATG 4 cut(s) 94, 150, 166, 378
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 4 cut(s) 133, 144, 278, 363
FokI GGATG 2 cut(s) 80, 548
Fsp4HI GCNGC 4 cut(s) 133, 144, 278, 363
FspBI CTAG 2 cut(s) 470, 524
GluI GCNGC 4 cut(s) 133, 144, 278, 363
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 1 cut(s) 427
Hin1II CATG 4 cut(s) 98, 154, 170, 382
HincII GTYRAC 2 cut(s) 375, 509
HindII GTYRAC 2 cut(s) 375, 509
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 226, 331, 400, 449
HpaI GTTAAC 1 cut(s) 375
Hpy166II GTNNAC 3 cut(s) 163, 375, 509
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 3 cut(s) 151, 203, 328
Hpy8I GTNNAC 3 cut(s) 163, 375, 509
HpyAV CCTTC 2 cut(s) 157, 526
HpyCH4V TGCA 8 cut(s) 44, 80, 163, 170, 261, 277, 355, 562
HpyF3I CTNAG 2 cut(s) 84, 116
Hsp92II CATG 4 cut(s) 98, 154, 170, 382
Ksp22I TGATCA 1 cut(s) 414
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 4 cut(s) 130, 144, 289, 374
LweI GCATC 1 cut(s) 89
MabI ACCWGGT 1 cut(s) 460
MaeI CTAG 2 cut(s) 470, 524
MaeIII GTNAC 1 cut(s) 317
MalI GATC 1 cut(s) 416
MboI GATC 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 2 cut(s) 290, 409
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 5 cut(s) 92, 266, 293, 417, 520
MseI TTAA 3 cut(s) 60, 240, 374
MspA1I CMGCKG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 462
NdeII GATC 1 cut(s) 414
NlaIII CATG 4 cut(s) 98, 154, 170, 382
NlaIV GGNNCC 1 cut(s) 14
PagI TCATGA 1 cut(s) 150
PceI AGGCCT 1 cut(s) 427
PfeI GAWTC 4 cut(s) 226, 331, 400, 449
PkrI GCNGC 4 cut(s) 134, 145, 279, 364
PpuMI RGGWCCY 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 2 cut(s) 12, 216
PspPPI RGGWCCY 1 cut(s) 12
PstI CTGCAG 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
SaqAI TTAA 3 cut(s) 60, 240, 374
SatI GCNGC 4 cut(s) 133, 144, 278, 363
Sau3AI GATC 1 cut(s) 414
Sau96I GGNCC 2 cut(s) 12, 216
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 165
SexAI ACCWGGT 1 cut(s) 460
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 2 cut(s) 12, 216
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 2 cut(s) 290, 409
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 1 cut(s) 567
SspMI CTAG 2 cut(s) 470, 524
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 460
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 1 cut(s) 233
TasI AATT 2 cut(s) 290, 409
TfiI GAWTC 4 cut(s) 226, 331, 400, 449
Tru1I TTAA 3 cut(s) 60, 240, 374
Tru9I TTAA 3 cut(s) 60, 240, 374
TscAI CASTG 1 cut(s) 133
TseI GCWGC 4 cut(s) 132, 143, 277, 362
TspDTI ATGAA 7 cut(s) 17, 139, 167, 323, 395, 455, 538
TspRI CASTG 1 cut(s) 133
VneI GTGCAC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 12, 216
XapI RAATTY 1 cut(s) 290
XspI CTAG 2 cut(s) 470, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.