pycom08g03130

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000194
Physical Location & Seq
Forward (+)
2463803 .. 2465274
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03130.2

Sequence Viewer

Length: 705 bp
ATGTCCAAAAACAATGCAAATGTAGCAGATTATGAACAAAAGGCGAGGAAAGCAAAATACATTGGATGGCTAGGGAAACTCTCGGCCTCCCGAAGTAAGGGAGGATTGGGTTTCAGGAACTTTCAGGAATTCAATGATGCTCTCCTTGCGAAACAGTGTTGGCGCCTCATCCAAGATCCTACGTCTCTATGGGCTAGAGTTTTAAAGGCAAGATATTTTCCCCATTGTTCGTTTCTTGATGCTAAAAGAGGAAGTCATGCTTCCTGGGTGTGGTCTAGCTTGTTGGTGGGCAGAGCCAGAGACCTTCTGGTGGGGGAAACGCATTGGCAAATCATGGGTGGAAGGGACGTGCGTGTCTGGGTCGATAAATGGATCCCCTCCCTTCCCGTGGGACATCATGTGCCGAGGGGTGAAGTTGCGATGTTGAGAAATACCCGAGTTGAGTTGCTAATGTGTACAAATAACAAATCTTGGGATATTGATTTTCTTATTGCCTTTTTATCAACGAAGGAGATGAATGCTATTCAGGACACAGTGATTGGTGATACTTCTAAAATGGACAGGCTGGTTTGGCCATTTGATAAACGTGGGCGATACACGGTGAAATCAGGGTACCATTGGGTACATTCAATGGTGCACGGTCCTGAAGGTCAACATTTATCCCATGGTAGGCAATTACCAGAACATCTTTGGAAAGTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

235

Amino Acids

26.99

Weight (kDa)

9.89

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc65I GGTACC 1 cut(s) 612
AccB1I GGYRCC 2 cut(s) 162, 612
AclWI GGATC 3 cut(s) 170, 367, 380
AcoI YGGCCR 1 cut(s) 572
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 3 cut(s) 457, 614, 624
AfiI CCNNNNNNNGG 5 cut(s) 97, 270, 310, 388, 669
AgsI TTSAA 2 cut(s) 133, 630
AjiI CACGTC 1 cut(s) 349
AjnI CCWGG 1 cut(s) 263
AluBI AGCT 1 cut(s) 279
AluI AGCT 1 cut(s) 279
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 2 cut(s) 189, 294
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 3 cut(s) 170, 367, 380
Ama87I CYCGRG 1 cut(s) 435
AoxI GGCC 2 cut(s) 84, 572
ApaLI GTGCAC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 612
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 641
AsuHPI GGTGA 3 cut(s) 422, 554, 613
AvaI CYCGRG 1 cut(s) 435
AvaII GGWCC 1 cut(s) 641
BaeGI GKGCMC 1 cut(s) 639
BalI TGGCCA 1 cut(s) 574
BamHI GGATCC 1 cut(s) 372
BanI GGYRCC 2 cut(s) 162, 612
Bbv12I GWGCWC 1 cut(s) 639
BccI CCATC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 189, 294
BfaI CTAG 3 cut(s) 71, 195, 276
BfoI RGCGCY 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 265
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 435
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 641
BmiI GGNNCC 3 cut(s) 164, 374, 614
BmrFI CCNGG 1 cut(s) 265
BmsI GCATC 2 cut(s) 127, 229
BsaHI GRCGYC 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 264, 387, 404, 664
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 270, 310, 388, 669
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 4 cut(s) 264, 387, 404, 664
BseGI GGATG 2 cut(s) 71, 168
BseLI CCNNNNNNNGG 5 cut(s) 97, 270, 310, 388, 669
BseSI GKGCMC 1 cut(s) 639
BshFI GGCC 2 cut(s) 86, 574
BshNI GGYRCC 2 cut(s) 162, 612
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 1 cut(s) 435
BslFI GGGAC 2 cut(s) 359, 405
BslI CCNNNNNNNGG 5 cut(s) 97, 270, 310, 388, 669
BsmAI GTCTC 2 cut(s) 189, 294
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 359, 405
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 86, 574
Bso31I GGTCTC 1 cut(s) 294
BsoBI CYCGRG 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 639
Bsp1407I TGTACA 1 cut(s) 455
Bsp143I GATC 2 cut(s) 175, 372
Bsp19I CCATGG 1 cut(s) 664
BspANI GGCC 2 cut(s) 86, 574
BspLI GGNNCC 3 cut(s) 164, 374, 614
BspPI GGATC 3 cut(s) 170, 367, 380
BspT107I GGYRCC 2 cut(s) 162, 612
BspTNI GGTCTC 1 cut(s) 294
BsrGI TGTACA 1 cut(s) 455
BssECI CCNNGG 4 cut(s) 264, 387, 404, 664
BssMI GATC 2 cut(s) 175, 372
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 664
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 4 cut(s) 156, 535, 601, 641
BstACI GRCGYC 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 455
BstDSI CCRYGG 2 cut(s) 387, 664
BstF5I GGATG 2 cut(s) 71, 168
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 2 cut(s) 178, 375
BstMAI GTCTC 2 cut(s) 189, 294
BstMBI GATC 2 cut(s) 175, 372
BstMWI GCNNNNNNNGC 4 cut(s) 23, 50, 146, 571
BstNI CCWGG 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 263
BstSLI GKGCMC 1 cut(s) 639
BstX2I RGATCY 2 cut(s) 175, 372
BstYI RGATCY 2 cut(s) 175, 372
BsuRI GGCC 2 cut(s) 86, 574
BtgI CCRYGG 2 cut(s) 387, 664
BtgZI GCGATG 1 cut(s) 434
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 2 cut(s) 71, 168
BtsIMutI CAGTG 2 cut(s) 161, 540
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 641
Csp6I GTAC 3 cut(s) 456, 613, 623
CviAII CATG 4 cut(s) 257, 334, 398, 665
CviJI RGCY 7 cut(s) 70, 86, 194, 279, 296, 565, 574
CviKI_1 RGCY 7 cut(s) 70, 86, 194, 279, 296, 565, 574
CviQI GTAC 3 cut(s) 456, 613, 623
DinI GGCGCC 1 cut(s) 164
DpnI GATC 2 cut(s) 177, 374
DpnII GATC 2 cut(s) 175, 372
DraI TTTAAA 1 cut(s) 204
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 664
Eco31I GGTCTC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 435
EcoRI GAATTC 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 664
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 4 cut(s) 260, 337, 401, 668
FaiI YATR 6 cut(s) 33, 190, 258, 335, 399, 666
FalI AAGNNNNNCTT 2 cut(s) 244, 276
FaqI GGGAC 2 cut(s) 359, 405
FatI CATG 4 cut(s) 256, 333, 397, 664
FokI GGATG 2 cut(s) 78, 155
FspBI CTAG 3 cut(s) 71, 195, 276
GlaI GCGC 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 2 cut(s) 86, 574
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 4 cut(s) 260, 337, 401, 668
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 653
HindII GTYRAC 1 cut(s) 653
HphI GGTGA 3 cut(s) 422, 554, 613
Hpy166II GTNNAC 3 cut(s) 456, 637, 653
Hpy188III TCNNGA 6 cut(s) 90, 115, 125, 236, 527, 644
Hpy8I GTNNAC 3 cut(s) 456, 637, 653
HpyAV CCTTC 5 cut(s) 314, 336, 392, 502, 641
HpyCH4III ACNGT 4 cut(s) 156, 535, 601, 641
HpyCH4IV ACGT 3 cut(s) 182, 348, 586
HpyCH4V TGCA 2 cut(s) 17, 637
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 50, 146, 571
HpySE526I ACGT 3 cut(s) 182, 348, 586
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 4 cut(s) 260, 337, 401, 668
HspAI GCGC 1 cut(s) 163
KasI GGCGCC 1 cut(s) 162
KpnI GGTACC 1 cut(s) 616
Kzo9I GATC 2 cut(s) 175, 372
LweI GCATC 2 cut(s) 127, 229
MaeI CTAG 3 cut(s) 71, 195, 276
MaeII ACGT 3 cut(s) 182, 348, 586
MalI GATC 2 cut(s) 177, 374
MboI GATC 2 cut(s) 175, 372
MflI RGATCY 2 cut(s) 175, 372
MhlI GDGCHC 1 cut(s) 639
MlsI TGGCCA 1 cut(s) 574
MluCI AATT 2 cut(s) 128, 674
MluNI TGGCCA 1 cut(s) 574
Mly113I GGCGCC 1 cut(s) 163
MnlI CCTC 7 cut(s) 39, 95, 97, 176, 242, 388, 399
Mox20I TGGCCA 1 cut(s) 574
MscI TGGCCA 1 cut(s) 574
MseI TTAA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 574
MspR9I CCNGG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 4 cut(s) 23, 50, 146, 571
NarI GGCGCC 1 cut(s) 163
NcoI CCATGG 1 cut(s) 664
NdeII GATC 2 cut(s) 175, 372
NlaIII CATG 4 cut(s) 260, 337, 401, 668
NlaIV GGNNCC 3 cut(s) 164, 374, 614
NmeAIII GCCGAG 2 cut(s) 62, 429
PctI GAATGC 1 cut(s) 523
PluTI GGCGCC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 164, 374, 614
PspPI GGNCC 1 cut(s) 641
PsuI RGATCY 2 cut(s) 175, 372
RsaI GTAC 3 cut(s) 457, 614, 624
RsaNI GTAC 3 cut(s) 456, 613, 623
SaqAI TTAA 1 cut(s) 203
Sau3AI GATC 2 cut(s) 175, 372
Sau96I GGNCC 1 cut(s) 641
ScrFI CCNGG 1 cut(s) 265
SduI GDGCHC 1 cut(s) 639
SetI ASST 6 cut(s) 185, 281, 306, 351, 589, 652
SfaNI GCATC 2 cut(s) 127, 229
SfoI GGCGCC 1 cut(s) 164
SinI GGWCC 1 cut(s) 641
Sse9I AATT 2 cut(s) 128, 674
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 3 cut(s) 71, 195, 276
StyD4I CCNGG 1 cut(s) 263
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 156, 535, 601, 641
TaiI ACGT 3 cut(s) 185, 351, 589
TaqI TCGA 1 cut(s) 363
TasI AATT 2 cut(s) 128, 674
TatI WGTACW 1 cut(s) 455
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 2 cut(s) 161, 540
TspDTI ATGAA 2 cut(s) 48, 530
TspRI CASTG 2 cut(s) 161, 540
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 687
XspI CTAG 3 cut(s) 71, 195, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.