RchiOBHm_Chr6g0249781

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000194
Physical Location & Seq
Forward (+)
5632800 .. 5637052
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22393

Sequence Viewer

Length: 4152 bp
ATGAGGCTATTATCGTGGAATTGTCAGGGTCTGGGGGCGGCCCTGACTGGAAAGTCTCTCCGACGTTTGGTTAAACGTCATAGCCCCTCTATGCTTTTTCTTATGGAGACTCGTCAGCAAGAGTCAGTGCTCAAGTTTTGGATGCGTCAACTTTGTTTTGACCATTACTTTGTCGTTGATCCTACTGACAATGGGGGTGGTGGTCTTGCTCTCTTTTGGGACAACTCGGTCCAAGTCTCCAGTATTATCGGCACGGTTAATTATATTAATACTAGTCTGTATTTTATTTCTGAAAATTTCCATTGTAATGTGTCTTGGTTGTATGGGAACCCCCATGTTAATCAAAAATCGGTCTTCTGGCGTTCTTTGTACACTGTCTTCTCCCCTCATGTTTTACATTGGTTGTGCTTGGGGGATTTTAACGAAATCATGTGGCTGCATGAAAAATGGGGTGGCCTTCCTCAGCCTCGGTGGAGGATTAATCTGTTTAAAAACTTCCTGAACCATGCCCACCTAATGGATCTTCTTTTTCAAGGGCCAGATTACACCTGGTACTGCTTCCAACATGGCAAGGTCGTTATCAAGGAGAGGTTGGACCGCGCCTTTGGCAACAATGCTTGGATTGCAAATCAGCCGACTTCCCAGGTTTTTCACCTTCCTTTACTTGGTTCTGATCATCGCCCAATCATGCTGGACACTAAACCCACAGAGCGTCTGACTCCGCCTATCTTCCGGTTCGAACACTTGTGGACCACACATCCAACTAGCACTCAGATTGTCCAAGCCAACTGGCAGAACTCTTCCTCCCCATCTCAGGCTACAAACTGGGTTTCAAATCTCAATGAGTGCCAAACTTCTCTATCCTCTTGGGCAAAAAATACCTTTCCCAATTTTGGCAAACTTGCATCTGATGGCCAAAGTAGAATCCAATCTCTTTTGGACTCTGATCTTCCTGATGCCCATCAACAAATCACTGCTGTGTCTGATGAAATTGCTTCCCATTGGGCCTTGGAGGAATTATATTGGAAACAGAGATCACGAGTCTCGTGGCTAGCCCATGGCGACCTCAACACCCGTTTCTTCCACCAAGCAACAATTCTGCGTCGTCGAAGAAATAAGATTCTGAGACTCCAAAACGATTTTGGCCACTGGTTAACAAATGAGAGAGATATTGTTGATCATCTCAATAACTTCTTTCGTGACATGTATGTTTCTTCTCCAACTGACCACGTGCAAACTGTTCTGGATTTTGTTGACCCGGTGGTAACCTCCGATATGAACAATGCTCTGTTGTCCGAAATTACCATTGCAGAAGTAAGGGATGCAGTTTTTGACATGGGTGCACTTAAAGCACCGGGCCCCGATGGCTTCCCTGGTACCTTCTACCAATCTTATTGGGAAATTGTCAAAAAAGTTGTTTTTGAAGCAACGTCTCATTCCTTTGCTTCTGCCTCTGTCTTGAACCAGTTCAATGCTACCTTCATTGCTTTGATTCCAAAGGTGGAAACCCCTCAGCAAGCTTCCCATTTTCGCCCTATTGCTCTCTGCAACTTTGCTTACAAGATCCTGACGAAGATCATTTCTACCAGATTACGGCCTTTTATGCCATCCCTAATTTCTGAGAATCAGTCTGCTTTTGTCTCTGGTAGACAGATTCAGGACAACATTTTGGTGGCCCATGAAATGTTTCACCACCTCAAGTTACTCAAGTCAGGTTCAGTCGGGGAATTTGCAATTAAACTGGACATGAACAAGGCCTATGATCGTGTGGACTGGAAGTTTTTGGAGCTGGTTCTTCTGAAGATGGGATTCCACCAAAATTGGGTGAGATTGGTAATGTCTTGTGTTACCTCTGTCTCAATGGCTATTATTGTCAACGGAGCTCCTGGTCACAAATTCAAACCAACCCGTGGTCTCAGGCAGGGAGATCCTCTCTCCCCTTTCCTCTTCCTTTTTATCAATGATGTTTTATCTACCATGATCCGGAAAATATGTGACGCTAACTTACTGGATGCTGTTAAATTGAGCTCGAATGGTCCAATGGTTAGTCATCTTTTCTTTGCAGATGATTCCATCTTTTTCCTCAAAGCTACACTGTACAATTGCGAAGTACTCTCCGATACTTTGCATCACTATTGTAGTGCCTCTGGTCAATCCATTAATCTCCACAAATCTTCTCTCTTCTTTAGTCCCAACACCACTCCAGAAATTGTTAACCTCATCAGCATGGTGTTGGGTATGCAACCAGTTTTGAATCCGGGCTTTTATCTTGGTCTGCCGACCATCTGGGGCCGATCAAAAGTTTCGGCTTTGGCTTATGTTAAAGAGCACATCGAAAAGAAAATTTCGGGGTGGAATACTTCCACTTTATCCCAAGCTGGAAAAGAAATCATGATCAAATCAGTGGCAATGGCAATCCCTGCCTACCCAATGTCGATCTTCAAATTCCCTTCTTCTCTCTGCAACCAAATCAACTCTGCGCTTGCAAATTTCTGGTGGGGAAATGACACCACTACGGGTATACACTGGAAAAATTGGTCTTCTCTGGGGTTGCCTAAATCTTGTGGAGGTATGGGTTTTCGTAGTATTGAATCTTTCAATTATGCCCTTCTTGCCAAACAGGGTTGGAGAATCCTTGCCAATCCCAATGCACTCTGGGTCCGTGTTCTGAAGAGTCGTTACTTCCCCTCCTGCACCTTCTTTGAAGCAACTCGGGGATCCTCACCATCTTGGGCTTGGGCCAGCATTTTGGATGCCAGGCATATTCTCTCGGATGGTTGTATTTGGCAGGTTGGATCTGGTCTTTCTATTGATTTCTGGAGAGACAATTGGATCCCCCGACTACCGTCTTGCAATTTGAAAGACTTTATGCCTCTCAACACCCCGGAAACCCCGGTCAGTTTGTTTGTCGACTCTCTGAATCACACATGGAATCTGGATGCGGTCTCTCCTTTCCTCTCAGTGGATGCTATCAGAGCTATTAACGCTACCCCTTTCGGAAATCCGGAAGATGAAGACTCTATTCTTTGGCCGCCAACCTCATCGGGCATTTACTCCGTTAGGACGGGTTATGAAGCAAAAATCAAAGACTCCATGCCCTCACTTGTTGTTCATCCTCACAGGTCGCACTCAATCCCCTCAAGCACTTGGAATTGGCTCTGGAATCTTAAGGTTACACCAAGAATCAAACACTTCCTATGGCGAGCTCTTTCGGGTGCTTTAGCAACAAACCACAACAGACATCAAAGGCACCTTGCATCTTCCCCACTCTGCACCTTCTGTGAGGCTCATCCTGAAACAATTGAACATGTTCTTTTATTATGCCCTTGGGCAAGAAGAACGTGGTTCTGCCATCCCCTCTCTTACAGGATTGACCCCCAAGCCGTGACTTCTCTTGATATTTGGATTTCCGCGATCTTCTCCCCGGATTCGTCATCATCTCTACCTGATCTGGTTTCCCAAACTCATGTGTTGTTCTTACTGTGGTCTATTTGGAAACATAGGTGCAATTGTGTATTCAACCATGATACCTCGGATTCTATTCTTACTGCTTCTCGAGCCTTCTCTGCTTCCATGGAATTTCTATCGATCCCCAAATGCCTCTCCATTCGTGGTGATGTGAGATCCTCCTCCTCTGAATCATCTGTTTCGGCAATTTGGAAACCCCCTCCCGTTGGTATGTTCAAGATAAACACTGATGCGGCTTGGAATGCGGATTCCTTATCTTGTGGTCTGGGTACCCTGATTCGTGATTCTAAGGGTGCTGTTCTTGGGGGGTTAGCCAAATCATCACATGCGATGTCCTCTCTGGCAGCAGAAGCCATGGCTATTGTCCTTGGTCTCTCTCTTGCCTCATCCCTCTCCCTTTCTTCTTTTCAGTTGGAATCAGACTCCTTGGTTTTGATTTCACATTTGTTAAACCCTCTTTCTTCGATTGATTGGTCTGCAACTCAAATCGTCTCTCGGATTCGAGCTATGGCTGGCCACTTCGATCGTGTTAACTGGCTTTGGACCAGTAGACATGCTAATTGTGCTGCGGACTCTATTGCGGCTCTGGCATCTCGCAGGGTGTGCACGGATGACTGGGTGTCCAATCCTCCCCCCTCCCTAATGCGAATTCTTCTCTTTGATGCAGCGGGTCCTCCCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1383

Amino Acids

155.89

Weight (kDa)

8.64

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 4 - 226 5.7e-12 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 502 - 729 1.7e-29 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1042 - 1115 1.4e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 1231 - 1352 1.9e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 52, 227
Acc36I ACCTGC 1 cut(s) 2779
Acc65I GGTACC 2 cut(s) 1376, 3737
AccB1I GGYRCC 3 cut(s) 1376, 3249, 3737
AccB7I CCANNNNNTGG 2 cut(s) 517, 1910
AccI GTMKAC 4 cut(s) 1648, 2521, 2910, 4018
AccII CGCG 2 cut(s) 600, 3413
AccIII TCCGGA 2 cut(s) 1983, 3004
AcoI YGGCCR 4 cut(s) 913, 1144, 3029, 3982
AcsI RAATTY 8 cut(s) 295, 1727, 1895, 2343, 2444, 2488, 3578, 4116
AcuI CTGAAG 2 cut(s) 1820, 2690
AcvI CACGTG 1 cut(s) 1231
AfaI GTAC 6 cut(s) 371, 554, 1378, 2099, 2112, 3739
AflII CTTAAG 1 cut(s) 3167
AflIII ACRYGT 2 cut(s) 1203, 3307
AhdI GACNNNNNGTC 1 cut(s) 4087
AhlI ACTAGT 1 cut(s) 272
AjnI CCWGG 5 cut(s) 548, 642, 1372, 1885, 2756
AjuI GAANNNNNNNTTGG 2 cut(s) 1080, 1112
AleI CACNNNNGTG 1 cut(s) 977
AloI GAACNNNNNNTCC 2 cut(s) 788, 820
AluBI AGCT 9 cut(s) 1520, 1789, 1883, 2028, 2090, 2378, 2978, 3206, 3974
AluI AGCT 9 cut(s) 1520, 1789, 1883, 2028, 2090, 2378, 2978, 3206, 3974
Alw21I GWGCWC 7 cut(s) 132, 1345, 1885, 2030, 2331, 3208, 4076
Alw44I GTGCAC 2 cut(s) 1341, 4072
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 2 cut(s) 2712, 3555
Aor13HI TCCGGA 2 cut(s) 1983, 3004
ApaI GGGCCC 1 cut(s) 1361
ApaLI GTGCAC 2 cut(s) 1341, 4072
ApeKI GCWGC 4 cut(s) 436, 3812, 4034, 4133
ApoI RAATTY 8 cut(s) 295, 1727, 1895, 2343, 2444, 2488, 3578, 4116
ArsI GACNNNNNNTTYG 4 cut(s) 152, 184, 587, 619
AseI ATTAAT 3 cut(s) 267, 480, 2160
Asp700I GAANNNNTTC 3 cut(s) 1466, 1686, 3309
Asp718I GGTACC 2 cut(s) 1376, 3737
AspLEI GCGC 2 cut(s) 602, 2482
AsuC2I CCSGG 6 cut(s) 1259, 1356, 2259, 2885, 2894, 3425
AsuHPI GGTGA 5 cut(s) 644, 1682, 1837, 2715, 3626
AsuII TTCGAA 1 cut(s) 738
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 2712, 3555
AvaII GGWCC 7 cut(s) 229, 595, 750, 2036, 2659, 4011, 4139
BaeGI GKGCMC 3 cut(s) 1345, 1361, 4076
BalI TGGCCA 3 cut(s) 915, 1146, 3984
BamHI GGATCC 2 cut(s) 2717, 2832
BanI GGYRCC 3 cut(s) 1376, 3249, 3737
BanII GRGCYC 4 cut(s) 1361, 1885, 2030, 3208
BarI GAAGNNNNNNTAC 4 cut(s) 362, 394, 2663, 2695
BauI CACGAG 2 cut(s) 1038, 1045
BbrPI CACGTG 1 cut(s) 1231
BbsI GAAGAC 4 cut(s) 346, 370, 2532, 3021
Bbv12I GWGCWC 7 cut(s) 132, 1345, 1885, 2030, 2331, 3208, 4076
BbvCI CCTCAGC 2 cut(s) 462, 1512
BbvI GCAGC 4 cut(s) 423, 3824, 4021, 4145
BceAI ACGGC 2 cut(s) 1610, 3368
BciT130I CCWGG 5 cut(s) 550, 644, 1374, 1887, 2758
BclI TGATCA 3 cut(s) 673, 1177, 2394
BcnI CCSGG 6 cut(s) 1259, 1356, 2259, 2885, 2894, 3425
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 273, 765, 1052
BfrI CTTAAG 1 cut(s) 3167
BfuAI ACCTGC 1 cut(s) 2779
BglI GCCNNNNNGGC 1 cut(s) 1365
BisI GCNGC 8 cut(s) 39, 437, 3032, 3702, 3813, 4035, 4050, 4134
BlsI GCNGC 8 cut(s) 40, 438, 3033, 3703, 3814, 4036, 4051, 4135
BmcAI AGTACT 1 cut(s) 2112
Bme18I GGWCC 7 cut(s) 229, 595, 750, 2036, 2659, 4011, 4139
BmeRI GACNNNNNGTC 1 cut(s) 4087
BmeT110I CYCGRG 2 cut(s) 2712, 3555
BmrI ACTGGG 2 cut(s) 835, 4093
BmtI GCTAGC 1 cut(s) 1055
BmuI ACTGGG 2 cut(s) 835, 4093
BoxI GACNNNNGTC 1 cut(s) 2845
BpiI GAAGAC 4 cut(s) 346, 370, 2532, 3021
BplI GAGNNNNNCTC 2 cut(s) 1917, 1949
BpmI CTGGAG 3 cut(s) 223, 2187, 2839
Bpu10I CCTNAGC 2 cut(s) 462, 1512
Bpu14I TTCGAA 1 cut(s) 738
BpuEI CTTGAG 4 cut(s) 116, 1682, 1691, 3124
BpuMI CCSGG 6 cut(s) 1259, 1356, 2259, 2885, 2894, 3425
Bsa29I ATCGAT 1 cut(s) 3587
BsaAI YACGTR 1 cut(s) 1231
BsaBI GATNNNNATC 1 cut(s) 960
BsaI GGTCTC 3 cut(s) 1919, 2950, 3845
BsaWI WCCGGW 3 cut(s) 732, 1983, 3004
BsaXI ACNNNNNCTCC 4 cut(s) 788, 818, 2672, 2702
Bse3DI GCAATG 3 cut(s) 1305, 1482, 2415
Bse8I GATNNNNATC 1 cut(s) 960
BseAI TCCGGA 2 cut(s) 1983, 3004
BseBI CCWGG 5 cut(s) 550, 644, 1374, 1887, 2758
BseCI ATCGAT 1 cut(s) 3587
BseJI GATNNNNATC 1 cut(s) 960
BseMI GCAATG 3 cut(s) 1305, 1482, 2415
BseMII CTCAG 8 cut(s) 476, 785, 827, 1115, 1526, 1611, 1930, 2973
BseRI GAGGAG 2 cut(s) 3619, 3622
BseSI GKGCMC 3 cut(s) 1345, 1361, 4076
BseXI GCAGC 4 cut(s) 423, 3824, 4021, 4145
BsgI GTGCAG 2 cut(s) 2677, 3256
Bsh1236I CGCG 2 cut(s) 600, 3413
Bsh1285I CGRYCG 1 cut(s) 3994
BshNI GGYRCC 3 cut(s) 1376, 3249, 3737
BshVI ATCGAT 1 cut(s) 3587
BsiEI CGRYCG 1 cut(s) 3994
BsiHKAI GWGCWC 7 cut(s) 132, 1345, 1885, 2030, 2331, 3208, 4076
BsiHKCI CYCGRG 2 cut(s) 2712, 3555
BsiSI CCGG 9 cut(s) 733, 1259, 1355, 1984, 2258, 2885, 2894, 3005, 3425
BslFI GGGAC 2 cut(s) 233, 2175
BsmBI CGTCTC 2 cut(s) 1437, 3964
BsmFI GGGAC 2 cut(s) 233, 2175
BsmI GAATGC 1 cut(s) 3715
Bso31I GGTCTC 3 cut(s) 1919, 2950, 3845
BsoBI CYCGRG 2 cut(s) 2712, 3555
Bsp119I TTCGAA 1 cut(s) 738
Bsp120I GGGCCC 1 cut(s) 1357
Bsp1286I GDGCHC 8 cut(s) 132, 1345, 1361, 1885, 2030, 2331, 3208, 4076
Bsp13I TCCGGA 2 cut(s) 1983, 3004
Bsp1407I TGTACA 2 cut(s) 369, 2097
Bsp19I CCATGG 3 cut(s) 1057, 3573, 3822
BspCNI CTCAG 8 cut(s) 475, 784, 826, 1116, 1525, 1612, 1929, 2972
BspDI ATCGAT 1 cut(s) 3587
BspEI TCCGGA 2 cut(s) 1983, 3004
BspFNI CGCG 2 cut(s) 600, 3413
BspHI TCATGA 1 cut(s) 2391
BspMI ACCTGC 1 cut(s) 2779
BspOI GCTAGC 1 cut(s) 1055
BspT104I TTCGAA 1 cut(s) 738
BspT107I GGYRCC 3 cut(s) 1376, 3249, 3737
BspTI CTTAAG 1 cut(s) 3167
BspTNI GGTCTC 3 cut(s) 1919, 2950, 3845
BsrDI GCAATG 3 cut(s) 1305, 1482, 2415
BsrGI TGTACA 2 cut(s) 369, 2097
BssNAI GTATAC 1 cut(s) 2522
BssSI CACGAG 2 cut(s) 1038, 1045
BssT1I CCWWGG 7 cut(s) 1008, 1057, 3326, 3573, 3822, 3835, 3894
Bst1107I GTATAC 1 cut(s) 2522
Bst2BI CACGAG 2 cut(s) 1038, 1045
Bst2UI CCWGG 5 cut(s) 550, 644, 1374, 1887, 2758
Bst4CI ACNGT 6 cut(s) 256, 376, 1240, 2097, 2847, 3483
Bst6I CTCTTC 4 cut(s) 805, 1952, 2186, 2666
BstAFI CTTAAG 1 cut(s) 3167
BstAPI GCANNNNNTGC 2 cut(s) 2420, 4071
BstAUI TGTACA 2 cut(s) 369, 2097
BstBAI YACGTR 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 6 cut(s) 1053, 1518, 2484, 2743, 3204, 3982
BstDEI CTNAG 9 cut(s) 462, 771, 813, 1124, 1512, 1620, 1916, 2959, 3756
BstDSI CCRYGG 4 cut(s) 1057, 1909, 3573, 3822
BstEII GGTNACC 1 cut(s) 1264
BstFNI CGCG 2 cut(s) 600, 3413
BstHHI GCGC 2 cut(s) 602, 2482
BstMCI CGRYCG 1 cut(s) 3994
BstNI CCWGG 5 cut(s) 550, 644, 1374, 1887, 2758
BstNSI RCATGY 4 cut(s) 1207, 3311, 3797, 4025
BstPAI GACNNNNGTC 1 cut(s) 2845
BstPI GGTNACC 1 cut(s) 1264
BstSLI GKGCMC 3 cut(s) 1345, 1361, 4076
BstUI CGCG 2 cut(s) 600, 3413
BstV1I GCAGC 4 cut(s) 423, 3824, 4021, 4145
BstV2I GAAGAC 4 cut(s) 346, 370, 2532, 3021
BstX2I RGATCY 7 cut(s) 520, 1563, 1927, 2717, 2795, 2832, 3623
BstXI CCANNNNNNTGG 1 cut(s) 2749
BstYI RGATCY 7 cut(s) 520, 1563, 1927, 2717, 2795, 2832, 3623
BstZ17I GTATAC 1 cut(s) 2522
Bsu15I ATCGAT 1 cut(s) 3587
BsuTUI ATCGAT 1 cut(s) 3587
BtgI CCRYGG 4 cut(s) 1057, 1909, 3573, 3822
BtgZI GCGATG 2 cut(s) 662, 3812
BtsI GCAGTG 1 cut(s) 972
BveI ACCTGC 1 cut(s) 2779
Cac8I GCNNGC 6 cut(s) 1053, 1518, 2484, 2743, 3204, 3982
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 1 cut(s) 2391
CfoI GCGC 2 cut(s) 602, 2482
ClaI ATCGAT 1 cut(s) 3587
CseI GACGC 4 cut(s) 134, 701, 1091, 2006
CsiI ACCWGGT 1 cut(s) 548
Csp6I GTAC 6 cut(s) 370, 553, 1377, 2098, 2111, 3738
CspCI CAANNNNNGTGG 4 cut(s) 178, 213, 1802, 1837
CviQI GTAC 6 cut(s) 370, 553, 1377, 2098, 2111, 3738
DdeI CTNAG 9 cut(s) 462, 771, 813, 1124, 1512, 1620, 1916, 2959, 3756
DraI TTTAAA 1 cut(s) 490
DrdI GACNNNNNNGTC 2 cut(s) 52, 227
DriI GACNNNNNGTC 1 cut(s) 4087
DseDI GACNNNNNNGTC 2 cut(s) 52, 227
EaeI YGGCCR 4 cut(s) 913, 1144, 3029, 3982
Eam1104I CTCTTC 4 cut(s) 805, 1952, 2186, 2666
Eam1105I GACNNNNNGTC 1 cut(s) 4087
EarI CTCTTC 4 cut(s) 805, 1952, 2186, 2666
EciI GGCGGA 1 cut(s) 711
Ecl136II GAGCTC 3 cut(s) 1883, 2028, 3206
Eco130I CCWWGG 7 cut(s) 1008, 1057, 3326, 3573, 3822, 3835, 3894
Eco147I AGGCCT 1 cut(s) 1757
Eco24I GRGCYC 4 cut(s) 1361, 1885, 2030, 3208
Eco31I GGTCTC 3 cut(s) 1919, 2950, 3845
Eco47I GGWCC 7 cut(s) 229, 595, 750, 2036, 2659, 4011, 4139
Eco53kI GAGCTC 3 cut(s) 1883, 2028, 3206
Eco57I CTGAAG 2 cut(s) 1820, 2690
Eco72I CACGTG 1 cut(s) 1231
Eco88I CYCGRG 2 cut(s) 2712, 3555
Eco91I GGTNACC 1 cut(s) 1264
EcoICRI GAGCTC 3 cut(s) 1883, 2028, 3206
EcoO109I RGGNCCY 2 cut(s) 1358, 4139
EcoO65I GGTNACC 1 cut(s) 1264
EcoRI GAATTC 1 cut(s) 4116
EcoRII CCWGG 5 cut(s) 548, 642, 1372, 1885, 2756
EcoT14I CCWWGG 7 cut(s) 1008, 1057, 3326, 3573, 3822, 3835, 3894
EcoT38I GRGCYC 4 cut(s) 1361, 1885, 2030, 3208
ErhI CCWWGG 7 cut(s) 1008, 1057, 3326, 3573, 3822, 3835, 3894
Esp3I CGTCTC 2 cut(s) 1437, 3964
FaqI GGGAC 2 cut(s) 233, 2175
FauI CCCGC 1 cut(s) 4129
FbaI TGATCA 3 cut(s) 673, 1177, 2394
FblI GTMKAC 4 cut(s) 1648, 2521, 2910, 4018
Fnu4HI GCNGC 8 cut(s) 39, 437, 3032, 3702, 3813, 4035, 4050, 4134
FriOI GRGCYC 4 cut(s) 1361, 1885, 2030, 3208
Fsp4HI GCNGC 8 cut(s) 39, 437, 3032, 3702, 3813, 4035, 4050, 4134
FspBI CTAG 3 cut(s) 273, 765, 1052
GlaI GCGC 2 cut(s) 601, 2481
GluI GCNGC 8 cut(s) 39, 437, 3032, 3702, 3813, 4035, 4050, 4134
GsuI CTGGAG 3 cut(s) 223, 2187, 2839
HapII CCGG 9 cut(s) 733, 1259, 1355, 1984, 2258, 2885, 2894, 3005, 3425
HgaI GACGC 4 cut(s) 134, 701, 1091, 2006
HhaI GCGC 2 cut(s) 602, 2482
Hin6I GCGC 2 cut(s) 600, 2480
HinP1I GCGC 2 cut(s) 600, 2480
HincII GTYRAC 7 cut(s) 149, 1155, 1255, 1876, 2215, 2911, 4000
HindII GTYRAC 7 cut(s) 149, 1155, 1255, 1876, 2215, 2911, 4000
HindIII AAGCTT 1 cut(s) 1518
HpaI GTTAAC 3 cut(s) 1155, 2215, 4000
HpaII CCGG 9 cut(s) 733, 1259, 1355, 1984, 2258, 2885, 2894, 3005, 3425
HphI GGTGA 5 cut(s) 644, 1682, 1837, 2715, 3626
Hpy99I CGWCG 3 cut(s) 66, 1107, 1110
HpyAV CCTTC 9 cut(s) 467, 665, 1390, 1489, 2460, 2618, 2707, 3286, 3571
HpyCH4III ACNGT 6 cut(s) 256, 376, 1240, 2097, 2847, 3483
HpyCH4IV ACGT 5 cut(s) 64, 76, 1230, 1430, 3341
HpyF3I CTNAG 9 cut(s) 462, 771, 813, 1124, 1512, 1620, 1916, 2959, 3756
HpySE526I ACGT 5 cut(s) 64, 76, 1230, 1430, 3341
HspAI GCGC 2 cut(s) 600, 2480
Kpn2I TCCGGA 2 cut(s) 1983, 3004
KpnI GGTACC 2 cut(s) 1380, 3741
Ksp22I TGATCA 3 cut(s) 673, 1177, 2394
KspAI GTTAAC 3 cut(s) 1155, 2215, 4000
LmnI GCTCC 3 cut(s) 1786, 1880, 1888
Lsp1109I GCAGC 4 cut(s) 423, 3824, 4021, 4145
MabI ACCWGGT 1 cut(s) 548
MaeI CTAG 3 cut(s) 273, 765, 1052
MaeII ACGT 5 cut(s) 64, 76, 1230, 1430, 3341
MaeIII GTNAC 9 cut(s) 1199, 1264, 1701, 1846, 1889, 1994, 2678, 3172, 3385
MfeI CAATTG 4 cut(s) 2101, 2827, 3300, 3508
MflI RGATCY 7 cut(s) 520, 1563, 1927, 2717, 2795, 2832, 3623
MhlI GDGCHC 8 cut(s) 132, 1345, 1361, 1885, 2030, 2331, 3208, 4076
MlsI TGGCCA 3 cut(s) 915, 1146, 3984
MluNI TGGCCA 3 cut(s) 915, 1146, 3984
MmeI TCCRAC 8 cut(s) 85, 573, 586, 785, 1244, 2606, 2773, 3861
Mox20I TGGCCA 3 cut(s) 915, 1146, 3984
MroI TCCGGA 2 cut(s) 1983, 3004
MroXI GAANNNNTTC 3 cut(s) 1466, 1686, 3309
MscI TGGCCA 3 cut(s) 915, 1146, 3984
MslI CAYNNNNRTG 4 cut(s) 306, 977, 1670, 2225
Msp20I TGGCCA 3 cut(s) 915, 1146, 3984
MspA1I CMGCKG 1 cut(s) 4136
MspCI CTTAAG 1 cut(s) 3167
MspI CCGG 9 cut(s) 733, 1259, 1355, 1984, 2258, 2885, 2894, 3005, 3425
MunI CAATTG 4 cut(s) 2101, 2827, 3300, 3508
Mva1269I GAATGC 1 cut(s) 3715
MvaI CCWGG 5 cut(s) 550, 644, 1374, 1887, 2758
MvnI CGCG 2 cut(s) 600, 3413
NciI CCSGG 6 cut(s) 1259, 1356, 2259, 2885, 2894, 3425
NcoI CCATGG 3 cut(s) 1057, 3573, 3822
NheI GCTAGC 1 cut(s) 1051
NmuCI GTSAC 4 cut(s) 1199, 1889, 1994, 3385
NspI RCATGY 4 cut(s) 1207, 3311, 3797, 4025
NspV TTCGAA 1 cut(s) 738
OliI CACNNNNGTG 1 cut(s) 977
PaeR7I CTCGAG 1 cut(s) 3555
PagI TCATGA 1 cut(s) 2391
PceI AGGCCT 1 cut(s) 1757
PciI ACATGT 2 cut(s) 1203, 3307
PctI GAATGC 1 cut(s) 3715
PdmI GAANNNNTTC 3 cut(s) 1466, 1686, 3309
PflMI CCANNNNNTGG 2 cut(s) 517, 1910
PkrI GCNGC 8 cut(s) 40, 438, 3033, 3703, 3814, 4036, 4051, 4135
Ple19I CGATCG 1 cut(s) 3994
PmaCI CACGTG 1 cut(s) 1231
PmlI CACGTG 1 cut(s) 1231
Ppu21I YACGTR 1 cut(s) 1231
PpuMI RGGWCCY 1 cut(s) 4139
PscI ACATGT 2 cut(s) 1203, 3307
PshAI GACNNNNGTC 1 cut(s) 2845
PshBI ATTAAT 3 cut(s) 267, 480, 2160
Psp124BI GAGCTC 3 cut(s) 1885, 2030, 3208
Psp5II RGGWCCY 1 cut(s) 4139
Psp6I CCWGG 5 cut(s) 548, 642, 1372, 1885, 2756
PspCI CACGTG 1 cut(s) 1231
PspEI GGTNACC 1 cut(s) 1264
PspGI CCWGG 5 cut(s) 548, 642, 1372, 1885, 2756
PspOMI GGGCCC 1 cut(s) 1357
PspPPI RGGWCCY 1 cut(s) 4139
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 7 cut(s) 520, 1563, 1927, 2717, 2795, 2832, 3623
PvuI CGATCG 1 cut(s) 3994
RsaI GTAC 6 cut(s) 371, 554, 1378, 2099, 2112, 3739
RsaNI GTAC 6 cut(s) 370, 553, 1377, 2098, 2111, 3738
RseI CAYNNNNRTG 4 cut(s) 306, 977, 1670, 2225
SacI GAGCTC 3 cut(s) 1885, 2030, 3208
SalI GTCGAC 1 cut(s) 2909
SatI GCNGC 8 cut(s) 39, 437, 3032, 3702, 3813, 4035, 4050, 4134
ScaI AGTACT 1 cut(s) 2112
SduI GDGCHC 8 cut(s) 132, 1345, 1361, 1885, 2030, 2331, 3208, 4076
SexAI ACCWGGT 1 cut(s) 548
Sfr274I CTCGAG 1 cut(s) 3555
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 7 cut(s) 229, 595, 750, 2036, 2659, 4011, 4139
SlaI CTCGAG 1 cut(s) 3555
SmiMI CAYNNNNRTG 4 cut(s) 306, 977, 1670, 2225
SmlI CTYRAG 6 cut(s) 131, 1697, 1706, 3139, 3167, 3555
SmoI CTYRAG 6 cut(s) 131, 1697, 1706, 3139, 3167, 3555
SpeI ACTAGT 1 cut(s) 272
SseBI AGGCCT 1 cut(s) 1757
SspMI CTAG 3 cut(s) 273, 765, 1052
SstI GAGCTC 3 cut(s) 1885, 2030, 3208
StuI AGGCCT 1 cut(s) 1757
StyI CCWWGG 7 cut(s) 1008, 1057, 3326, 3573, 3822, 3835, 3894
TaaI ACNGT 6 cut(s) 256, 376, 1240, 2097, 2847, 3483
TaiI ACGT 5 cut(s) 67, 79, 1233, 1433, 3344
TaqII GACCGA 2 cut(s) 217, 340
TatI WGTACW 3 cut(s) 369, 2097, 2110
TauI GCSGC 4 cut(s) 41, 3034, 3704, 4052
TscAI CASTG 9 cut(s) 132, 379, 979, 1154, 2100, 2409, 2531, 2967, 3700
TseFI GTSAC 4 cut(s) 1199, 1889, 1994, 3385
TseI GCWGC 4 cut(s) 436, 3812, 4034, 4133
Tsp45I GTSAC 4 cut(s) 1199, 1889, 1994, 3385
TspDTI ATGAA 9 cut(s) 456, 1002, 1292, 1471, 1695, 1763, 3027, 3087, 3101
TspGWI ACGGA 4 cut(s) 1893, 2651, 3046, 4091
TspRI CASTG 9 cut(s) 132, 379, 979, 1154, 2100, 2409, 2531, 2967, 3700
Van91I CCANNNNNTGG 2 cut(s) 517, 1910
Vha464I CTTAAG 1 cut(s) 3167
VneI GTGCAC 2 cut(s) 1341, 4072
VpaK11BI GGWCC 7 cut(s) 229, 595, 750, 2036, 2659, 4011, 4139
VspI ATTAAT 3 cut(s) 267, 480, 2160
XapI RAATTY 8 cut(s) 295, 1727, 1895, 2343, 2444, 2488, 3578, 4116
XceI RCATGY 4 cut(s) 1207, 3311, 3797, 4025
XcmI CCANNNNNNNNNTGG 3 cut(s) 546, 1139, 2733
XhoI CTCGAG 1 cut(s) 3555
XmiI GTMKAC 4 cut(s) 1648, 2521, 2910, 4018
XmnI GAANNNNTTC 3 cut(s) 1466, 1686, 3309
XspI CTAG 3 cut(s) 273, 765, 1052
ZrmI AGTACT 1 cut(s) 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.