Rmu_sc0010099.1_g000002

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010099.1
OG0000194
Physical Location & Seq
Reverse (-)
2264 .. 3214
951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010099.1_g000002.1.cds

Sequence Viewer

Length: 951 bp
atggcaatccctgcctatccaatgtccgtcttcaagttcccaactactctttgcaaccaaatcaactctgcgcttgcaaacttctggtggggaaatgatacttctacgggtatacattggaagaattggtcttctcttggcttccctaaatcttgtggaggtatgggttttcgtactatcgagtctttcaatttagcccttcttgccaaacaaggatggagaatcctggccaatcccgacgctctctgggtccctgttctgaagaatcattactttcctcgctgctccttctttgaagcaactcggggctcctctccgtcttgggcttgggctagtattttggatgccaggcacattctctcggggggatgtatatggcaggttggatctggtcaatcgattgatttctggctggacaattggatccctagattaccttccttcaacttgaaagactttatgcctcagaatgctctggatactcctgttagtttattcgttgactccctgaaccatatctggaatttggatgtggtctctcctcttctccctgctgctgcaataagagctatcagagacacccctttcggaaacccggaggatattgactctattctttgggcggcaacttatgtgggtatctattctgttaagtcgggttatgaagcaaaaatcaaggtttccaggcatccatttgttgtacaccctaacaggtcgcactcaattcattctaatacttggaattggctttggaaacttaaggtcacaccacggatcaaacacttcctctggcgagctctctctggtgctttggcaacgaaccacaacagatatcaaaggcaccttgcttcctccccaatttgctccctctgtgaggcttatcctgaaacaattgagcatgctctactattatgcccatgggctagaagaacatggttctgtaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

316

Amino Acids

35.98

Weight (kDa)

9.4

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 840
AccI GTMKAC 1 cut(s) 112
AciI CCGC 1 cut(s) 623
AclWI GGATC 4 cut(s) 394, 418, 431, 782
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 523
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 2 cut(s) 175, 702
AfiI CCNNNNNNNGG 1 cut(s) 874
AflII CTTAAG 1 cut(s) 758
AgsI TTSAA 5 cut(s) 34, 190, 296, 445, 451
AjnI CCWGG 3 cut(s) 225, 347, 683
AluBI AGCT 2 cut(s) 569, 797
AluI AGCT 2 cut(s) 569, 797
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 2 cut(s) 541, 570
AlwI GGATC 4 cut(s) 394, 418, 431, 782
Ama87I CYCGRG 2 cut(s) 303, 361
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 3 cut(s) 282, 554, 557
ApoI RAATTY 1 cut(s) 523
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 596
AvaI CYCGRG 2 cut(s) 303, 361
AvaII GGWCC 1 cut(s) 250
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 423
BanI GGYRCC 1 cut(s) 840
BanII GRGCYC 2 cut(s) 311, 799
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BbsI GAAGAC 2 cut(s) 22, 123
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 3 cut(s) 269, 541, 544
BccI CCATC 1 cut(s) 210
BciT130I CCWGG 3 cut(s) 227, 349, 685
BciVI GTATCC 1 cut(s) 472
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 2 cut(s) 541, 570
BfaI CTAG 3 cut(s) 333, 429, 924
BfrI CTTAAG 1 cut(s) 758
BfuAI ACCTGC 1 cut(s) 370
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 4 cut(s) 283, 555, 558, 624
BlsI GCNGC 4 cut(s) 284, 556, 559, 625
Bme1390I CCNGG 4 cut(s) 227, 349, 596, 685
Bme18I GGWCC 1 cut(s) 250
BmeT110I CYCGRG 2 cut(s) 303, 361
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 5 cut(s) 251, 252, 310, 425, 842
BmrFI CCNGG 4 cut(s) 227, 349, 596, 685
BmsI GCATC 2 cut(s) 334, 697
BpiI GAAGAC 2 cut(s) 22, 123
BpuMI CCSGG 1 cut(s) 596
Bsa29I ATCGAT 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 541
BsaJI CCNNGG 2 cut(s) 770, 917
Bsc4I CCNNNNNNNGG 1 cut(s) 874
BseBI CCWGG 3 cut(s) 227, 349, 685
BseCI ATCGAT 1 cut(s) 398
BseDI CCNNGG 2 cut(s) 770, 917
BseGI GGATG 5 cut(s) 221, 349, 374, 535, 688
BseLI CCNNNNNNNGG 1 cut(s) 874
BseMII CTCAG 1 cut(s) 479
BseRI GAGGAG 2 cut(s) 301, 531
BseXI GCAGC 3 cut(s) 269, 541, 544
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 840
BshVI ATCGAT 1 cut(s) 398
BsiHKAI GWGCWC 1 cut(s) 799
BsiHKCI CYCGRG 2 cut(s) 303, 361
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 874
BsmAI GTCTC 2 cut(s) 541, 570
BsmFI GGGAC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 541
BsoBI CYCGRG 2 cut(s) 303, 361
Bsp1286I GDGCHC 2 cut(s) 311, 799
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 3 cut(s) 386, 423, 774
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 1 cut(s) 623
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 478
BspDI ATCGAT 1 cut(s) 398
BspLI GGNNCC 5 cut(s) 251, 252, 310, 425, 842
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 4 cut(s) 394, 418, 431, 782
BspT107I GGYRCC 1 cut(s) 840
BspTI CTTAAG 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 541
BsrGI TGTACA 1 cut(s) 700
BssECI CCNNGG 2 cut(s) 770, 917
BssMI GATC 3 cut(s) 386, 423, 774
BssNAI GTATAC 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 917
Bst1107I GTATAC 1 cut(s) 113
Bst2UI CCWGG 3 cut(s) 227, 349, 685
Bst6I CTCTTC 1 cut(s) 549
BstAFI CTTAAG 1 cut(s) 758
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 700
BstC8I GCNNGC 3 cut(s) 75, 795, 900
BstDEI CTNAG 1 cut(s) 465
BstDSI CCRYGG 2 cut(s) 770, 917
BstENI CCTNNNNNAGG 1 cut(s) 872
BstF5I GGATG 5 cut(s) 221, 349, 374, 535, 688
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 3 cut(s) 389, 426, 777
BstMAI GTCTC 2 cut(s) 541, 570
BstMBI GATC 3 cut(s) 386, 423, 774
BstMWI GCNNNNNNNGC 3 cut(s) 11, 203, 566
BstNI CCWGG 3 cut(s) 227, 349, 685
BstNSI RCATGY 1 cut(s) 902
BstSCI CCNGG 4 cut(s) 225, 347, 594, 683
BstV1I GCAGC 3 cut(s) 269, 541, 544
BstV2I GAAGAC 2 cut(s) 22, 123
BstX2I RGATCY 2 cut(s) 386, 423
BstYI RGATCY 2 cut(s) 386, 423
BstZ17I GTATAC 1 cut(s) 113
Bsu15I ATCGAT 1 cut(s) 398
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 1 cut(s) 230
BsuTUI ATCGAT 1 cut(s) 398
BtgI CCRYGG 2 cut(s) 770, 917
BtsCI GGATG 5 cut(s) 221, 349, 374, 535, 688
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 3 cut(s) 75, 795, 900
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 398
CseI GACGC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 174, 701
CspCI CAANNNNNGTGG 2 cut(s) 615, 650
CviAII CATG 3 cut(s) 899, 918, 933
CviQI GTAC 2 cut(s) 174, 701
DdeI CTNAG 1 cut(s) 465
DpnI GATC 3 cut(s) 388, 425, 776
DpnII GATC 3 cut(s) 386, 423, 774
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Ecl136II GAGCTC 1 cut(s) 797
Eco130I CCWWGG 1 cut(s) 917
Eco24I GRGCYC 2 cut(s) 311, 799
Eco31I GGTCTC 1 cut(s) 541
Eco32I GATATC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 797
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 303, 361
EcoICRI GAGCTC 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 872
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 3 cut(s) 225, 347, 683
EcoRV GATATC 1 cut(s) 833
EcoT14I CCWWGG 1 cut(s) 917
EcoT38I GRGCYC 2 cut(s) 311, 799
ErhI CCWWGG 1 cut(s) 917
FaeI CATG 3 cut(s) 902, 921, 936
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 898, 917, 932
FblI GTMKAC 1 cut(s) 112
Fnu4HI GCNGC 4 cut(s) 283, 555, 558, 624
FokI GGATG 5 cut(s) 228, 356, 381, 542, 675
FriOI GRGCYC 2 cut(s) 311, 799
Fsp4HI GCNGC 4 cut(s) 283, 555, 558, 624
FspBI CTAG 3 cut(s) 333, 429, 924
GlaI GCGC 1 cut(s) 72
GluI GCNGC 4 cut(s) 283, 555, 558, 624
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 596
HgaI GACGC 1 cut(s) 248
HhaI GCGC 1 cut(s) 73
Hin1II CATG 3 cut(s) 902, 921, 936
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 5 cut(s) 182, 222, 265, 503, 608
HpaII CCGG 1 cut(s) 596
Hpy166II GTNNAC 3 cut(s) 113, 502, 703
Hpy188I TCNGA 4 cut(s) 261, 468, 575, 590
Hpy188III TCNNGA 4 cut(s) 236, 476, 520, 884
Hpy8I GTNNAC 3 cut(s) 113, 502, 703
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 209, 298, 447, 451
HpyCH4V TGCA 3 cut(s) 54, 77, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 203, 566
HpyF3I CTNAG 1 cut(s) 465
Hsp92II CATG 3 cut(s) 902, 921, 936
HspAI GCGC 1 cut(s) 71
KflI GGGWCCC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 386, 423, 774
LmnI GCTCC 3 cut(s) 290, 314, 869
Lsp1109I GCAGC 3 cut(s) 269, 541, 544
LweI GCATC 2 cut(s) 334, 697
MaeI CTAG 3 cut(s) 333, 429, 924
MaeIII GTNAC 1 cut(s) 763
MalI GATC 3 cut(s) 388, 425, 776
MboI GATC 3 cut(s) 386, 423, 774
MboII GAAGA 6 cut(s) 22, 123, 133, 274, 536, 939
MfeI CAATTG 2 cut(s) 418, 891
MflI RGATCY 2 cut(s) 386, 423
MhlI GDGCHC 2 cut(s) 311, 799
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 8 cut(s) 124, 190, 418, 523, 723, 742, 858, 891
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 3 cut(s) 191, 497, 602
MmeI TCCRAC 1 cut(s) 364
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 651, 759
Msp20I TGGCCA 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 4 cut(s) 227, 349, 596, 685
MunI CAATTG 2 cut(s) 418, 891
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 3 cut(s) 227, 349, 685
MwoI GCNNNNNNNGC 3 cut(s) 11, 203, 566
NciI CCSGG 1 cut(s) 596
NcoI CCATGG 1 cut(s) 917
NdeII GATC 3 cut(s) 386, 423, 774
NlaIII CATG 3 cut(s) 902, 921, 936
NlaIV GGNNCC 5 cut(s) 251, 252, 310, 425, 842
NmuCI GTSAC 1 cut(s) 763
NspI RCATGY 1 cut(s) 902
PaeI GCATGC 1 cut(s) 902
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 2 cut(s) 222, 265
PkrI GCNGC 4 cut(s) 284, 556, 559, 625
PleI GAGTC 3 cut(s) 190, 497, 602
PpsI GAGTC 3 cut(s) 190, 497, 602
PpuMI RGGWCCY 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 799
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 3 cut(s) 225, 347, 683
PspGI CCWGG 3 cut(s) 225, 347, 683
PspN4I GGNNCC 5 cut(s) 251, 252, 310, 425, 842
PspPI GGNCC 1 cut(s) 250
PspPPI RGGWCCY 1 cut(s) 250
PsuI RGATCY 2 cut(s) 386, 423
RsaI GTAC 2 cut(s) 175, 702
RsaNI GTAC 2 cut(s) 174, 701
SacI GAGCTC 1 cut(s) 799
SaqAI TTAA 2 cut(s) 651, 759
SatI GCNGC 4 cut(s) 283, 555, 558, 624
Sau3AI GATC 3 cut(s) 386, 423, 774
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 3 cut(s) 191, 497, 602
ScrFI CCNGG 4 cut(s) 227, 349, 596, 685
SduI GDGCHC 2 cut(s) 311, 799
SetI ASST 9 cut(s) 163, 384, 439, 571, 681, 716, 765, 799, 846
SfaNI GCATC 2 cut(s) 334, 697
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SphI GCATGC 1 cut(s) 902
Sse9I AATT 8 cut(s) 124, 190, 418, 523, 723, 742, 858, 891
SsiI CCGC 1 cut(s) 623
SspMI CTAG 3 cut(s) 333, 429, 924
SstI GAGCTC 1 cut(s) 799
StyD4I CCNGG 4 cut(s) 225, 347, 594, 683
StyI CCWWGG 1 cut(s) 917
TaqI TCGA 2 cut(s) 180, 398
TasI AATT 8 cut(s) 124, 190, 418, 523, 723, 742, 858, 891
TatI WGTACW 1 cut(s) 700
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 2 cut(s) 222, 265
Tru1I TTAA 2 cut(s) 651, 759
Tru9I TTAA 2 cut(s) 651, 759
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 3 cut(s) 282, 554, 557
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 2 cut(s) 678, 716
TspGWI ACGGA 3 cut(s) 16, 306, 787
Vha464I CTTAAG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 250
XagI CCTNNNNNAGG 1 cut(s) 872
XapI RAATTY 1 cut(s) 523
XceI RCATGY 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 112
XspI CTAG 3 cut(s) 333, 429, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.