pycom07g26560

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000194
Physical Location & Seq
Forward (+)
26545351 .. 26550847
5497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g26560.2

Sequence Viewer

Length: 5289 bp
ATGGCGACTAGTGGGGTGGAGGAACTGGTGGTTCAACTGAACCAAACACTGGAGTTATCCACTATGGAGCAGGGAGTTAAATTGGTTGGGAAGGTCTTTACGCTGAAACCAGTGAACAAATGGGGAGTCAGGAATATTCTTAGAGCGGCTTGGAAAGAATTGGGTGAGGTGGAAATAAAATGGGTAAGGGAGAATGTCTTTATTATATCGGTCAAAGATGAACATGTGGCTTCAAAGATCATTGAGCAGGTCCCCTGGGCAGTGATGAAGAAGGTATTCTCAGTCGTGAAATGGCCTCCGGAGCTCGCCTTAGAGGAGCTGGAGTTGAATGCTGTTCCATTTTGGATTCAAATAAGGGGAATTCCGCTAGGCTTAGCATCTGTTGAGAATGTTCGGCGTGTAATCAAAGAAGCTGGGGAATTTATAGCCATGGAGGATCCCGGACACGCTCGTGGTTTTGTCCGAGTAAGAATCTCAGTTGATACGGAGAAGCCTTTATTTAAGGGATGCTGGATTCGAAGGGAGTCAAATAAGGAAACCTGGGTGGAATTTCGGTATGAACGGCTTCAAGATTTTTGCTACAAGTGTGGACGGATTGGGCATATCAATACAGAATGCTCCGCTGAGATGTCTGGAGAAGGGGAGGTAGCATATGGAGAGTGGATTAAGGCGCCGCCGATAAGAGAGGTGGTGATACCAAAGAGAGTGGAGTGTGTGGGCAGGGGGGAACGCCGGCAGGCCGGGGCGGTGCGCGGACCGGCCTTCACGTCTGCACAGAATCAAGATAGATTAGGGATGTCAAACTTACAAAGAAGGGAGCGCAATCAAACCATTGAGACTGGGGGAGCATCTAAGAGTCGTGGCACAGATAAAAAAAAATGGCGTAGAAGAATGCGGGGGGAGGGAGAGGATTCGTCTTCCTGCTCTGAAATGGAAAGCATCAAAACAGTTGGTGAAGGACAATCGGGCGATGTTCAGGAGGGTAATGATACTCTGTCCGTCCAGGGTAATGACCCACTCTGGGGCAGTAAAAGATGTGGGGATGCACAGGATTTGATGCTAACCCAAGCTCCACAGAAGAAACTCAAGGGACCGGTTACGGAGGAGAACTGCCAAGGTAGGGCGGAAGAGATAGGAAGGAAGTTAAACAGCAAGGAGGGAGGGATAACGATGGAAAGCGTGAACCGTACGGCGGAGGAAAATAAGGAAGAAATAAGTGAGAATACTGGAAGACAGGATGTAATGGAAGGGGGGTCGGATTTAGATAAAGGTAGGATGGTGACACGGAAGGCGGGCAAGTGGGAGTTATATCTTCTGGAACTGTCGTGGGCTTGGGTCGGACACGACAGTCAGGGCTCTCCATGGGCTTATTCGAAAAAAGAGACCCTCTATGATCGATCCTCGGGCTCATCGGAGACTAAGATGAAAGATCATAGAATTCTAGGGGTGAGAAGGAGATTGGGTTATGTTCATGGCTTTGATGTTTCGCCAGTTGGAGTTTCTGGAGGATTGAGCTTGTGGTGGGAGGACAATCTGGAGGTTAATATTATCTTTTCGTCCAAGAATATTATTGATGCTGTGATGAAAATTAAAGGGCAAACGCATTGGAGTCGGATTACGGGGGTGTACGGAACGCCGTATAGAGTTGAAAAAAATCTGTTTTGGGAATGGATGGTTAACCACTTTACCCCAACGGACATTCCTTGGATTTGTGGCGGGGATTTCAACGAATTCCTGTGGGATCATGAGAAGTCTGGTGGTGTTGAGGTTCTGTACAACAGGCCAAGGTTCTTAGAAGAGTTTCTGTCTTCCTCCCAGCTGATGGATTTGGGTTTCAATGGGCCGGCCTTTACGTGGAGAGGATTGAGAAAAAGGGATTGGGTGGAGGAGAGGTTAGACAGGGTTATGGCGAATGAAAAGTGGCAGCAACTCTGGCCGAACGCCCAAGTTATGCATGAAACGGTCTTAGCATCAGACCACTGCCCAGTTATTCTTATTTCTAATATTGAAGGGCAGAAAGGAAGGAAGATATTTAGGTTTGAGGCGTATTGGGTCTCGGAGGAAGAGTGTAAGAATTTGGTGGAACAGTGCTGGGATAGGCGGCATAATGGCAGCCCTGTGAATAGGTGGGTGAGATCTTTAAACGATTGCCGTTATCGTTTAAGCAGGTGGAATCGCAATAAGTTTATGGGGCGAGGGAATAGGATCCATGATCTTCTTTCCCAGTTAGATTTGCTCCAGCGGGACTGGGGTCCAAACTATGATGAGATAAGGGAGATTTCTCGGCGGATTGATGAATTAAGACTCCAAGAGGAGAGTTATTGGTGCCAGCGATCGAGGGTAAAATGGTTGAGGGAGGGGGATGCCAACACTCAGTTTTTTCACTCTTCAACCCTTCAGAGGCGAAGAAGAAATAAGATAGTGAAGCTTAGGGATGAGAATGGGAACTGGGTGGAGAATCCGGCTAAGGTGCGCCAATTGGTGGATAATCACTTCATTTCAGTTTTTAGCTCGGCAGGGGATCGTAACTGGGGGTCGCTGCTAGATTGCATGAATCCCTCGGTCTCGCCAGAAATGAATGAGGCGCTAATTGCACCAGTCACGGAGGAGGAGATAAAGGCGGCGGCTGGGAATATGGGAGGGCTTAAGGCTCCTGGCCCCGACGGTTTCCAGGGGATCTTTTATCAGACATATTGGGAGATTGTGAGGGAGGGTGTTTCGGCTTTGGTTAGAGATTTGATTCAGGATGCTGCGGGTTCGAGTTTGATAAATCAGACTCATGTGGTGCTGATTCCCAAAGTCCCGAACCCGGAAGTTGTGTCACAATTCAGGCCGATTAGCCTGTGCAACTACTCGTACAAAATTCTGTCAAAGATTCTTGCTAACCGGTTGAAGGTATTGTTACCCAAAATCATCTCTCCTTCTCAGAATGCTTTTGTGGCGGGTAGACAAATTCAGGATTGTATTGGCATAGCCCATGAGATGTTTCATTATCTGAAGGGAAGGAAAGCTCGGAATAGATTTGAAATGGGGATTAAACTTGATATGCAGAAGGCGTATGATAGGGTTGAATGGGACTTTTTGGATGCGGTTATGGAAAGAATGGGTTTTTGCAGTAGCTGGAGATCGCTAATTAATGGGTGTATTTCATCTGTGAAGTTCGCTGTTCTCCTAAATGGGCAGGCTGGGAAGTCTTTTGCTCCCTCGCGGGGTCTCCGACAGGGGGATCCTCTTTCTCCTTATCTATTTATTTTGATGGGGGAAGTTCTATCCAAGCTGATTCAGGGTGCGGTTGACCAAGGTAGATTGGAAGGTGTTAAGATGGGTGGTTCCGGACCAGTGATTTCTCACCTCTTCTTTGCGGATGATACCCTTCTGTTTTTGCGGGCTGATATGAAAAACTGCCGGAATTTGAGACATCTGCTGGACAATTTCTGTGTCGCGTCGGGGCAGAAGGTCAATTTGGAGAAATCCAGTGTATTTTTTGGGGCTAATGTTCCGAAGGTGAATGCTGATCAGATGGGGAACGCCCTTGGTATGAAGGTTGTAATCAATCCGGGAACATATTTGGGGGTCCCTACCATATGGGCCGCTGTTTGTAAGGAATTGGATGCGCTGGTGACTGGGTTTTGGTGGGGATGCAAGGAGGGAGCCCACAAGATTCACTGGGTTTCAAAGGAGGTTTTGGGTTTGCCAAAAGACATGGGTGGTTTAGGCTTCAGAAATTTTCAGGATTTTAATGATGCTCTGCTTGCTAAACAGTGCTGGCGCTTAATCACGGAACCGGATTCGCTGTGGGCTAAGGTGATTAAGGCTCGGTATTTTCCACATAGCTCAATCTGGGAGGCGAAGAAGGGTGGGAGGGCCTCTTGGGCGTGGAGCAGCCTCATCTGTGGCAGGGACTTGATTAGGGAGGGCTCCCATTGGCAGATTTTGGGTGGTCAGGAGGTGCGGGTGTGGCAGGATAGGTGGCTTCCATCCTTGCCCCTTGGACACCCGGTGCCGGTTGGCCAGGTTACGGTTACTCCCAGTTTAAGGGTCAGTGCACTTATCTGTCCGGAGTCGGGGCGGTGGAATATTAACTTTTTACAGCCTTTCATATCGGGGGAGGCTATGCAGGCTATTGAGGCGACGCCTCTTGGAGATTTAAGTCGTAAAGATAGGCTTATCTGGGATATGAGTAAGAACGGCTCTTATTCGGTCAAGTCGGGATACAGATGGATGCAAGGAAGATCGCTGGCGAGAAGGGATTTGCGCCGGCCTTCTGTTCGTGGGGTTCCCAAAGCGTTTTGGAAGGGGATTTGGAAGCTGGAGGTGCCCCCCAAGTTGCGGCATTTCTTGTGGCTTTCTGTTCACAATTGTCTTCCTACTCGGGATGCACTGTTCCGACGAAGGTCCTCTCAAACCTCTAATTGTCCTATTTGCTGCTGCCATGATGAAACTATTGAGCACATTTTCCTTTCCTGCTCTTGGGTTGAGCCAATCTGGTTTGGAGGGGCTTTGGGTTATAAAATGGATCGTCTTTCTCTTCCTTCTTGGTCGGACTGGCTTCAGGGGGTTTTCTCTCCGAATTTTTGCAACTCTGGAGATATAATATGGAGACAATCTTATATTGTTTTTACCTGTTGGTGCATATGGAAGGCTCGCTGTGATTTTATTTTCAAAGGGGTGCCCATTAACCCGTTAAAGGTCCTGGCTGATGTCTCTGCGGCGGTGAGCTCGTTTTTTGGTGCAATGGCAAAGGCTGGGGATAGTGGAAGGGGTGACGGCAGGAGGAACTCTCAGGTTTCGAGATGGTGTGCTCCGGCCTACCCTTTTGTGAAGATTAATGTGGATGCTAGCTGGTCCAAGGCTTCAAAGATGGGTTTTGCTGGATTAATTGTGAGGGACATGGAGAATAAGTTCGTGGCTGCTGCGCGGCATCCTATTACTGCTCCTTCTGCTGCAGCAGCGGAGGCTTATGCGTTGTTGCACGGGTGCAGACTAGGTGCAGAGCTGGGTGTAAGGTATGTAATTCTGGAATCCGACTCTCTGGATGCGATTAAATGTCTGTCTACCTCGCTGTCAATGGGTAGTTGGGAGGCTTACCCGGTGCTGGCTAGGGTTAAACAGTTGGGGGGGGATTTCATTGATTGTCGATGGTCTTGGGTGCCACGGTCTGCTAATGGTGTGGCTCACAAGATTGCATCGATTGGTTTTACGGAGATGAGTGATGTCGTTTGGGTCGTTAGGCCCCCATCTTCGTTGGTCTATGTATTGAACAATGACGGATTACCTTGTCCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1763

Amino Acids

199.54

Weight (kDa)

9.01

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4509
AarI CACCTGC 1 cut(s) 2157
AasI GACNNNNNNGTC 1 cut(s) 1347
Acc36I ACCTGC 2 cut(s) 238, 2157
AccB1I GGYRCC 6 cut(s) 670, 2325, 4000, 4315, 4669, 5149
AccB7I CCANNNNNTGG 3 cut(s) 49, 1822, 2482
AccBSI CCGCTC 1 cut(s) 146
AccI GTMKAC 2 cut(s) 2947, 5054
AccII CGCG 4 cut(s) 753, 3208, 3443, 4918
AccIII TCCGGA 3 cut(s) 298, 3332, 4057
AcoI YGGCCR 2 cut(s) 1934, 4009
AcuI CTGAAG 4 cut(s) 2381, 3017, 3703, 4535
AcyI GRCGYC 2 cut(s) 671, 4133
AdeI CACNNNGTG 1 cut(s) 4000
AfaI GTAC 4 cut(s) 1189, 1628, 1775, 2858
AflII CTTAAG 1 cut(s) 2645
AflIII ACRYGT 1 cut(s) 223
AgeI ACCGGT 2 cut(s) 1093, 2886
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 768
AjnI CCWGG 7 cut(s) 254, 539, 1002, 2653, 2670, 4011, 4692
AjuI GAANNNNNNNTTGG 4 cut(s) 1861, 1893, 3446, 3478
AleI CACNNNNGTG 1 cut(s) 450
AloI GAACNNNNNNTCC 4 cut(s) 4367, 4399, 4886, 4918
Alw21I GWGCWC 5 cut(s) 306, 4048, 4452, 4721, 4804
Alw26I GTCTC 9 cut(s) 830, 1376, 1409, 2059, 2569, 3218, 3409, 4594, 4708
Alw44I GTGCAC 1 cut(s) 4044
AlwNI CAGNNNCTG 1 cut(s) 3120
Ama87I CYCGRG 2 cut(s) 1402, 4371
Aor13HI TCCGGA 3 cut(s) 298, 3332, 4057
ApaLI GTGCAC 1 cut(s) 4044
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AseI ATTAAT 3 cut(s) 3135, 4827, 4877
AsiGI ACCGGT 2 cut(s) 1093, 2886
Asp700I GAANNNNTTC 4 cut(s) 275, 564, 1800, 4901
AspLEI GCGC 9 cut(s) 673, 753, 822, 2475, 2587, 3616, 3771, 4257, 4918
AsuC2I CCSGG 6 cut(s) 441, 742, 2810, 3558, 3998, 5090
AsuII TTCGAA 2 cut(s) 517, 1373
AsuNHI GCTAGC 1 cut(s) 4838
AvaI CYCGRG 2 cut(s) 1402, 4371
AvaII GGWCC 9 cut(s) 250, 755, 1091, 2252, 3335, 3574, 4395, 4690, 4845
BaeGI GKGCMC 3 cut(s) 4048, 4320, 4674
BalI TGGCCA 1 cut(s) 4011
BamHI GGATCC 3 cut(s) 436, 2205, 3226
BanI GGYRCC 6 cut(s) 670, 2325, 4000, 4315, 4669, 5149
BanII GRGCYC 6 cut(s) 306, 1358, 1409, 3655, 3920, 4721
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 4 cut(s) 909, 1237, 1800, 4355
Bbv12I GWGCWC 5 cut(s) 306, 4048, 4452, 4721, 4804
BceAI ACGGC 6 cut(s) 578, 1206, 1621, 2136, 4204, 4783
BcgI CGANNNNNNTGC 2 cut(s) 1592, 1626
BciT130I CCWGG 7 cut(s) 256, 541, 1004, 2655, 2672, 4013, 4694
BciVI GTATCC 1 cut(s) 4205
BclI TGATCA 1 cut(s) 3514
BcnI CCSGG 6 cut(s) 441, 742, 2810, 3558, 3998, 5090
BcoDI GTCTC 9 cut(s) 830, 1376, 1409, 2059, 2569, 3218, 3409, 4594, 4708
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 7 cut(s) 9, 368, 1442, 2543, 4839, 4985, 5100
BfmI CTRYAG 1 cut(s) 4944
BfoI RGCGCY 3 cut(s) 674, 2588, 3772
BfrI CTTAAG 1 cut(s) 2645
BfuAI ACCTGC 2 cut(s) 238, 2157
BfuI GTATCC 1 cut(s) 4205
BglI GCCNNNNNGGC 1 cut(s) 3872
BglII AGATCT 1 cut(s) 2135
BlpI GCTNAGC 1 cut(s) 373
Bme18I GGWCC 9 cut(s) 250, 755, 1091, 2252, 3335, 3574, 4395, 4690, 4845
BmeT110I CYCGRG 2 cut(s) 1402, 4371
BmgBI CACGTC 1 cut(s) 768
BmrI ACTGGG 9 cut(s) 849, 1979, 2218, 2257, 2458, 2539, 3633, 3676, 4023
BmtI GCTAGC 1 cut(s) 4842
BmuI ACTGGG 9 cut(s) 849, 1979, 2218, 2257, 2458, 2539, 3633, 3676, 4023
BoxI GACNNNNGTC 2 cut(s) 2250, 4393
BpiI GAAGAC 4 cut(s) 909, 1237, 1800, 4355
BplI GAGNNNNNCTC 2 cut(s) 4765, 4797
BpmI CTGGAG 9 cut(s) 71, 341, 654, 1524, 1556, 2222, 3142, 4331, 4605
Bpu10I CCTNAGC 3 cut(s) 2429, 2466, 3801
Bpu1102I GCTNAGC 1 cut(s) 373
Bpu14I TTCGAA 2 cut(s) 517, 1373
BpuEI CTTGAG 1 cut(s) 1070
BpuMI CCSGG 6 cut(s) 441, 742, 2810, 3558, 3998, 5090
Bsa29I ATCGAT 2 cut(s) 1396, 5189
BsaAI YACGTR 1 cut(s) 1854
BsaHI GRCGYC 2 cut(s) 671, 4133
BsaI GGTCTC 4 cut(s) 1376, 2059, 2569, 3218
BsaWI WCCGGW 6 cut(s) 298, 1093, 2886, 3332, 3784, 4057
Bse118I RCCGGY 7 cut(s) 732, 757, 1093, 1843, 2886, 4003, 4257
Bse3DI GCAATG 1 cut(s) 4740
BseAI TCCGGA 3 cut(s) 298, 3332, 4057
BseBI CCWGG 7 cut(s) 256, 541, 1004, 2655, 2672, 4013, 4694
BseCI ATCGAT 2 cut(s) 1396, 5189
BseMI GCAATG 1 cut(s) 4740
BseMII CTCAG 6 cut(s) 294, 489, 615, 2387, 2939, 4796
BseRI GAGGAG 6 cut(s) 329, 1118, 1901, 2327, 2621, 2624
BseSI GKGCMC 3 cut(s) 4048, 4320, 4674
BseYI CCCAGC 7 cut(s) 413, 1815, 2091, 2627, 3185, 4745, 4996
BsgI GTGCAG 3 cut(s) 756, 4999, 5010
Bsh1236I CGCG 4 cut(s) 753, 3208, 3443, 4918
Bsh1285I CGRYCG 1 cut(s) 2336
BshNI GGYRCC 6 cut(s) 670, 2325, 4000, 4315, 4669, 5149
BshTI ACCGGT 2 cut(s) 1093, 2886
BshVI ATCGAT 2 cut(s) 1396, 5189
BsiEI CGRYCG 1 cut(s) 2336
BsiHKAI GWGCWC 5 cut(s) 306, 4048, 4452, 4721, 4804
BsiHKCI CYCGRG 2 cut(s) 1402, 4371
BsiWI CGTACG 1 cut(s) 1187
BslFI GGGAC 8 cut(s) 236, 1104, 2258, 2786, 3089, 3560, 3914, 4901
BsmAI GTCTC 9 cut(s) 830, 1376, 1409, 2059, 2569, 3218, 3409, 4594, 4708
BsmFI GGGAC 8 cut(s) 236, 1104, 2258, 2786, 3089, 3560, 3914, 4901
BsmI GAATGC 5 cut(s) 334, 620, 897, 2935, 3514
Bso31I GGTCTC 4 cut(s) 1376, 2059, 2569, 3218
BsoBI CYCGRG 2 cut(s) 1402, 4371
Bsp119I TTCGAA 2 cut(s) 517, 1373
Bsp13I TCCGGA 3 cut(s) 298, 3332, 4057
Bsp1407I TGTACA 1 cut(s) 1773
Bsp1720I GCTNAGC 1 cut(s) 373
Bsp19I CCATGG 2 cut(s) 429, 1361
BspCNI CTCAG 6 cut(s) 293, 488, 616, 2386, 2938, 4795
BspDI ATCGAT 2 cut(s) 1396, 5189
BspEI TCCGGA 3 cut(s) 298, 3332, 4057
BspFNI CGCG 4 cut(s) 753, 3208, 3443, 4918
BspHI TCATGA 1 cut(s) 1744
BspMAI CTGCAG 1 cut(s) 4948
BspMI ACCTGC 2 cut(s) 238, 2157
BspOI GCTAGC 1 cut(s) 4842
BspT104I TTCGAA 2 cut(s) 517, 1373
BspT107I GGYRCC 6 cut(s) 670, 2325, 4000, 4315, 4669, 5149
BspTI CTTAAG 1 cut(s) 2645
BspTNI GGTCTC 4 cut(s) 1376, 2059, 2569, 3218
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 4740
BsrFI RCCGGY 7 cut(s) 732, 757, 1093, 1843, 2886, 4003, 4257
BsrGI TGTACA 1 cut(s) 1773
BssAI RCCGGY 7 cut(s) 732, 757, 1093, 1843, 2886, 4003, 4257
BssNI GRCGYC 2 cut(s) 671, 4133
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 9 cut(s) 429, 1114, 1361, 1703, 1784, 3298, 3532, 3988, 4848
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 7 cut(s) 256, 541, 1004, 2655, 2672, 4013, 4694
Bst6I CTCTTC 6 cut(s) 1122, 1791, 2058, 2392, 3359, 4533
BstACI GRCGYC 2 cut(s) 671, 4133
BstAFI CTTAAG 1 cut(s) 2645
BstAUI TGTACA 1 cut(s) 1773
BstBAI YACGTR 1 cut(s) 1854
BstBI TTCGAA 2 cut(s) 517, 1373
BstDSI CCRYGG 3 cut(s) 429, 1361, 5153
BstFNI CGCG 4 cut(s) 753, 3208, 3443, 4918
BstH2I RGCGCY 3 cut(s) 674, 2588, 3772
BstHHI GCGC 9 cut(s) 673, 753, 822, 2475, 2587, 3616, 3771, 4257, 4918
BstMAI GTCTC 9 cut(s) 830, 1376, 1409, 2059, 2569, 3218, 3409, 4594, 4708
BstMCI CGRYCG 1 cut(s) 2336
BstNI CCWGG 7 cut(s) 256, 541, 1004, 2655, 2672, 4013, 4694
BstNSI RCATGY 1 cut(s) 227
BstPAI GACNNNNGTC 2 cut(s) 2250, 4393
BstSFI CTRYAG 1 cut(s) 4944
BstSLI GKGCMC 3 cut(s) 4048, 4320, 4674
BstUI CGCG 4 cut(s) 753, 3208, 3443, 4918
BstV2I GAAGAC 4 cut(s) 909, 1237, 1800, 4355
BstX2I RGATCY 5 cut(s) 436, 2135, 2205, 2676, 3226
BstYI RGATCY 5 cut(s) 436, 2135, 2205, 2676, 3226
Bsu15I ATCGAT 2 cut(s) 1396, 5189
BsuI GTATCC 1 cut(s) 4205
BsuTUI ATCGAT 2 cut(s) 1396, 5189
BtgI CCRYGG 3 cut(s) 429, 1361, 5153
BtgZI GCGATG 1 cut(s) 984
BtrI CACGTC 1 cut(s) 768
BtsI GCAGTG 2 cut(s) 267, 1978
BveI ACCTGC 2 cut(s) 238, 2157
CaiI CAGNNNCTG 1 cut(s) 3120
CciI TCATGA 1 cut(s) 1744
CfoI GCGC 9 cut(s) 673, 753, 822, 2475, 2587, 3616, 3771, 4257, 4918
Cfr10I RCCGGY 7 cut(s) 732, 757, 1093, 1843, 2886, 4003, 4257
ClaI ATCGAT 2 cut(s) 1396, 5189
CpoI CGGWCCG 1 cut(s) 755
CseI GACGC 2 cut(s) 3432, 4141
Csp6I GTAC 4 cut(s) 1188, 1627, 1774, 2857
CspAI ACCGGT 2 cut(s) 1093, 2886
CspCI CAANNNNNGTGG 2 cut(s) 687, 722
CspI CGGWCCG 1 cut(s) 755
CviQI GTAC 4 cut(s) 1188, 1627, 1774, 2857
DinI GGCGCC 1 cut(s) 672
DraI TTTAAA 1 cut(s) 2142
DraIII CACNNNGTG 1 cut(s) 4000
DrdI GACNNNNNNGTC 1 cut(s) 1347
DseDI GACNNNNNNGTC 1 cut(s) 1347
EaeI YGGCCR 2 cut(s) 1934, 4009
Eam1104I CTCTTC 6 cut(s) 1122, 1791, 2058, 2392, 3359, 4533
EarI CTCTTC 6 cut(s) 1122, 1791, 2058, 2392, 3359, 4533
EciI GGCGGA 3 cut(s) 1139, 1208, 2302
Ecl136II GAGCTC 2 cut(s) 304, 4719
Eco130I CCWWGG 9 cut(s) 429, 1114, 1361, 1703, 1784, 3298, 3532, 3988, 4848
Eco24I GRGCYC 6 cut(s) 306, 1358, 1409, 3655, 3920, 4721
Eco31I GGTCTC 4 cut(s) 1376, 2059, 2569, 3218
Eco47I GGWCC 9 cut(s) 250, 755, 1091, 2252, 3335, 3574, 4395, 4690, 4845
Eco53kI GAGCTC 2 cut(s) 304, 4719
Eco57I CTGAAG 4 cut(s) 2381, 3017, 3703, 4535
Eco88I CYCGRG 2 cut(s) 1402, 4371
EcoICRI GAGCTC 2 cut(s) 304, 4719
EcoO109I RGGNCCY 6 cut(s) 250, 3574, 3864, 4395, 4690, 5232
EcoRI GAATTC 3 cut(s) 360, 1437, 1730
EcoRII CCWGG 7 cut(s) 254, 539, 1002, 2653, 2670, 4011, 4692
EcoT14I CCWWGG 9 cut(s) 429, 1114, 1361, 1703, 1784, 3298, 3532, 3988, 4848
EcoT22I ATGCAT 1 cut(s) 1956
EcoT38I GRGCYC 6 cut(s) 306, 1358, 1409, 3655, 3920, 4721
EgeI GGCGCC 1 cut(s) 672
EheI GGCGCC 1 cut(s) 672
ErhI CCWWGG 9 cut(s) 429, 1114, 1361, 1703, 1784, 3298, 3532, 3988, 4848
FalI AAGNNNNNCTT 2 cut(s) 4149, 4181
FaqI GGGAC 8 cut(s) 236, 1104, 2258, 2786, 3089, 3560, 3914, 4901
FauI CCCGC 9 cut(s) 888, 1285, 1709, 2235, 2746, 2935, 3201, 3378, 3945
FauNDI CATATG 3 cut(s) 652, 3584, 4634
FbaI TGATCA 1 cut(s) 3514
FblI GTMKAC 2 cut(s) 2947, 5054
FriOI GRGCYC 6 cut(s) 306, 1358, 1409, 3655, 3920, 4721
FseI GGCCGGCC 1 cut(s) 1847
FspBI CTAG 7 cut(s) 9, 368, 1442, 2543, 4839, 4985, 5100
GlaI GCGC 9 cut(s) 672, 752, 821, 2474, 2586, 3615, 3770, 4256, 4917
GsaI CCCAGC 7 cut(s) 417, 1819, 2095, 2631, 3189, 4749, 5000
GsuI CTGGAG 9 cut(s) 71, 341, 654, 1524, 1556, 2222, 3142, 4331, 4605
HaeII RGCGCY 3 cut(s) 674, 2588, 3772
HgaI GACGC 2 cut(s) 3432, 4141
HhaI GCGC 9 cut(s) 673, 753, 822, 2475, 2587, 3616, 3771, 4257, 4918
Hin1I GRCGYC 2 cut(s) 671, 4133
Hin6I GCGC 9 cut(s) 671, 751, 820, 2473, 2585, 3614, 3769, 4255, 4916
HinP1I GCGC 9 cut(s) 671, 751, 820, 2473, 2585, 3614, 3769, 4255, 4916
HincII GTYRAC 2 cut(s) 1678, 3295
HindII GTYRAC 2 cut(s) 1678, 3295
HindIII AAGCTT 1 cut(s) 2426
HpaI GTTAAC 1 cut(s) 1678
Hpy99I CGWCG 4 cut(s) 2666, 3448, 4135, 4392
HpyCH4IV ACGT 2 cut(s) 767, 1853
HpySE526I ACGT 2 cut(s) 767, 1853
Hsp92I GRCGYC 2 cut(s) 671, 4133
HspAI GCGC 9 cut(s) 671, 751, 820, 2473, 2585, 3614, 3769, 4255, 4916
KasI GGCGCC 1 cut(s) 670
KflI GGGWCCC 1 cut(s) 3574
Kpn2I TCCGGA 3 cut(s) 298, 3332, 4057
KroI GCCGGC 3 cut(s) 732, 1843, 4257
KroNI GCCGGC 3 cut(s) 734, 1845, 4259
Ksp22I TGATCA 1 cut(s) 3514
KspAI GTTAAC 1 cut(s) 1678
MaeI CTAG 7 cut(s) 9, 368, 1442, 2543, 4839, 4985, 5100
MaeII ACGT 2 cut(s) 767, 1853
MbiI CCGCTC 1 cut(s) 146
MfeI CAATTG 2 cut(s) 2477, 4357
MflI RGATCY 5 cut(s) 436, 2135, 2205, 2676, 3226
MlsI TGGCCA 1 cut(s) 4011
MluNI TGGCCA 1 cut(s) 4011
Mly113I GGCGCC 1 cut(s) 671
MlyI GAGTC 8 cut(s) 135, 533, 865, 1618, 2298, 2770, 4070, 5021
MmeI TCCRAC 8 cut(s) 1236, 1318, 1474, 1592, 3241, 4411, 4521, 5049
Mox20I TGGCCA 1 cut(s) 4011
Mph1103I ATGCAT 1 cut(s) 1956
MroI TCCGGA 3 cut(s) 298, 3332, 4057
MroNI GCCGGC 3 cut(s) 732, 1843, 4257
MroXI GAANNNNTTC 4 cut(s) 275, 564, 1800, 4901
MscI TGGCCA 1 cut(s) 4011
MslI CAYNNNNRTG 1 cut(s) 450
Msp20I TGGCCA 1 cut(s) 4011
MspA1I CMGCKG 5 cut(s) 623, 1819, 2242, 3593, 4952
MspCI CTTAAG 1 cut(s) 2645
MunI CAATTG 2 cut(s) 2477, 4357
Mva1269I GAATGC 5 cut(s) 334, 620, 897, 2935, 3514
MvaI CCWGG 7 cut(s) 256, 541, 1004, 2655, 2672, 4013, 4694
MvnI CGCG 4 cut(s) 753, 3208, 3443, 4918
NaeI GCCGGC 3 cut(s) 734, 1845, 4259
NarI GGCGCC 1 cut(s) 671
NciI CCSGG 6 cut(s) 441, 742, 2810, 3558, 3998, 5090
NcoI CCATGG 2 cut(s) 429, 1361
NdeI CATATG 3 cut(s) 652, 3584, 4634
NgoMIV GCCGGC 3 cut(s) 732, 1843, 4257
NheI GCTAGC 1 cut(s) 4838
NmeAIII GCCGAG 2 cut(s) 2263, 2492
NmuCI GTSAC 5 cut(s) 1279, 2599, 2820, 3619, 4763
NsiI ATGCAT 1 cut(s) 1956
NspI RCATGY 1 cut(s) 227
NspV TTCGAA 2 cut(s) 517, 1373
OliI CACNNNNGTG 1 cut(s) 450
PagI TCATGA 1 cut(s) 1744
PaqCI CACCTGC 1 cut(s) 2157
PasI CCCWGGG 1 cut(s) 255
PciI ACATGT 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 5223
PctI GAATGC 5 cut(s) 334, 620, 897, 2935, 3514
PdiI GCCGGC 3 cut(s) 734, 1845, 4259
PdmI GAANNNNTTC 4 cut(s) 275, 564, 1800, 4901
Pfl23II CGTACG 1 cut(s) 1187
PflMI CCANNNNNTGG 3 cut(s) 49, 1822, 2482
PfoI TCCNGGA 2 cut(s) 439, 3556
PinAI ACCGGT 2 cut(s) 1093, 2886
Ple19I CGATCG 1 cut(s) 2336
PleI GAGTC 8 cut(s) 134, 532, 864, 1617, 2298, 2770, 4069, 5021
PluTI GGCGCC 1 cut(s) 674
PpsI GAGTC 8 cut(s) 134, 532, 864, 1617, 2298, 2770, 4069, 5021
Ppu21I YACGTR 1 cut(s) 1854
PpuMI RGGWCCY 4 cut(s) 250, 3574, 4395, 4690
PscI ACATGT 1 cut(s) 223
PshAI GACNNNNGTC 2 cut(s) 2250, 4393
PshBI ATTAAT 3 cut(s) 3135, 4827, 4877
PsiI TTATAA 1 cut(s) 4509
Psp124BI GAGCTC 2 cut(s) 306, 4721
Psp5II RGGWCCY 4 cut(s) 250, 3574, 4395, 4690
Psp6I CCWGG 7 cut(s) 254, 539, 1002, 2653, 2670, 4011, 4692
PspFI CCCAGC 7 cut(s) 413, 1815, 2091, 2627, 3185, 4745, 4996
PspGI CCWGG 7 cut(s) 254, 539, 1002, 2653, 2670, 4011, 4692
PspLI CGTACG 1 cut(s) 1187
PspPPI RGGWCCY 4 cut(s) 250, 3574, 4395, 4690
PstI CTGCAG 1 cut(s) 4948
PstNI CAGNNNCTG 1 cut(s) 3120
PsuI RGATCY 5 cut(s) 436, 2135, 2205, 2676, 3226
PvuI CGATCG 1 cut(s) 2336
PvuII CAGCTG 1 cut(s) 1819
RigI GGCCGGCC 1 cut(s) 1847
RsaI GTAC 4 cut(s) 1189, 1628, 1775, 2858
RsaNI GTAC 4 cut(s) 1188, 1627, 1774, 2857
RseI CAYNNNNRTG 1 cut(s) 450
Rsr2I CGGWCCG 1 cut(s) 755
RsrII CGGWCCG 1 cut(s) 755
SacI GAGCTC 2 cut(s) 306, 4721
SchI GAGTC 8 cut(s) 135, 533, 865, 1618, 2298, 2770, 4070, 5021
SfcI CTRYAG 1 cut(s) 4944
SfoI GGCGCC 1 cut(s) 672
SfuI TTCGAA 2 cut(s) 517, 1373
SinI GGWCC 9 cut(s) 250, 755, 1091, 2252, 3335, 3574, 4395, 4690, 4845
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 1085, 2645
SmoI CTYRAG 2 cut(s) 1085, 2645
SpeI ACTAGT 1 cut(s) 8
SspDI GGCGCC 1 cut(s) 670
SspI AATATT 5 cut(s) 136, 1546, 1567, 2005, 4078
SspMI CTAG 7 cut(s) 9, 368, 1442, 2543, 4839, 4985, 5100
SstI GAGCTC 2 cut(s) 306, 4721
StyI CCWWGG 9 cut(s) 429, 1114, 1361, 1703, 1784, 3298, 3532, 3988, 4848
TaiI ACGT 2 cut(s) 770, 1856
TaqI TCGA 8 cut(s) 517, 1373, 1396, 2336, 2759, 4790, 5137, 5189
TaqII GACCGA 3 cut(s) 199, 2551, 4189
TatI WGTACW 1 cut(s) 1773
TauI GCSGC 9 cut(s) 149, 676, 2104, 2624, 2627, 3593, 4333, 4712, 4921
TseFI GTSAC 5 cut(s) 1279, 2599, 2820, 3619, 4763
Tsp45I GTSAC 5 cut(s) 1279, 2599, 2820, 3619, 4763
Van91I CCANNNNNTGG 3 cut(s) 49, 1822, 2482
Vha464I CTTAAG 1 cut(s) 2645
VneI GTGCAC 1 cut(s) 4044
VpaK11BI GGWCC 9 cut(s) 250, 755, 1091, 2252, 3335, 3574, 4395, 4690, 4845
VspI ATTAAT 3 cut(s) 3135, 4827, 4877
XceI RCATGY 1 cut(s) 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 117
XmiI GTMKAC 2 cut(s) 2947, 5054
XmnI GAANNNNTTC 4 cut(s) 275, 564, 1800, 4901
XspI CTAG 7 cut(s) 9, 368, 1442, 2543, 4839, 4985, 5100
Zsp2I ATGCAT 1 cut(s) 1956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.