Rroxscaffold_1G00058460

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000194
Physical Location & Seq
Reverse (-)
80353754 .. 80360113
6360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058460.1

Sequence Viewer

Length: 1632 bp
ATGAATGCTTCCTTGTTGTCTGATGTTACTTTGGTGGAGGTCAAAACAGCTATCTTTGATTTGGGACCTCTTAAATCCCCAGGTCCTGATGGTTTTTCGGGCATCTTCTATCACTCGTATTGGGAAACAGTTCAACAAGTAGTTTTTGTAGTGGCTGGTTCTTTTCTTCATACTGGTACTCTGCTCCCGGAAATGAATAGAACATATATTACTTTAATTCCTAAGATAAAGGCGCAGGAACATGCTTCTCAATTTCGTCCTATTGGTCTTTGTAATTTCGCTTATAAGGTTCTTTCAAAGGTTCTTGCGAATCGGTTAGAACCTTTTATGCCAGAGCTTATTTCAGAGAACCAAGCTGCCTTTGTAACTGGTCGTCAAATTCTGGATAATTTGGTTGTGGCCCATGAATTTTTTCACCATCTTAAACTTCTTCGCAGCATTGGTGGTGAAGCTTTTGGTTTGAAATTGGATATTTCAAAGGCCTATGATTGTGTTGAGTGGGGTTTCCTACAAGCTGTACTGCTCAAACTGGGCTTTGCAGATCACTGGGTTATGCTGATTATGAATTGTATTCGCTCGGTTTCCCTCTCCATTCTGATCAATGGAAAACCGTCTGCTTTCTTCACTCCCACTCGAGGTATCCGCCAGGGGGATCCTTTATCTCCATACCTCTTTCTCTTTGTCACTGATGTGCTTTCCACTATGGTTCGTAAGGCTTGTGGAATGATGTGGTTGGTTCCTATGCAGTTATCTGTCTCCGGACCTCCCATTAGTCATTTGTTTTTCGCAGATGACTCTTTGTTTTTCTTCCAGGCCTCTGAGAAAAATGCTTCCACTTTGGTGTTTCTGTTGAATTCTTATAGCAAAGCCTCTAGTCAGCAGATCAACTATGCCAAGTCTCTACTTTATTTCAGCCCTAATACTTCTGAGGCACCCCAGCAGGTCATCAAAACCACTCTTAAGGTACATTCAATGGACAATCCTGGTTCCTACTTGGGTTTACCGACTATTTGGGGCCGCTCAAAACGTTCTGCTTTAGGCTATGTTCGTGATGCTGTGCTTTGCAAGATTCAAGGCTGGAAACAAGCTTCTTTGTCACCGGCGGGGAAGGAAATTATGATCAAGTCGGTTGCTTTGGCGATTCCAGCTTACCCCATGAATGTTTTCAAATTTCCAAAATCTCTTTGCACTGACATCAATAACGCTCTTGCAAAATTTTGGTGGGGTTCCAATGGTGATAAAGGCATTCACTGGAAGAAGTGGGAGCAGTTATGTTTATCGAAGTCCGTAGGTGGCCTTGGCTTTCGAAATTTGGAAGATTTCAACCTTTCTCTTTTGGCAAAACAGGGCTGGCGATTGCTTCACAACCCTTCTGCTATTTGGGCTCAAGTTCTCAAGCACAAGTATTTTCCCCACACCACCTTCTGGGAAGCTAAACAAAGCCATTCCTCTTCCTGGGTTTGGGCTAGTTTGTTAGCAGGCAAAGCCGTTTTGTCAAAAAAGGATCTTTATGGCGTGTTGGCAATGGTCTTCTTATTGATGCATGGAAGGATCAGTGGATTCCGAATCACTCGAGTTTTCGTCACCGGTTCCCTCATCTTCCTTCTCCAAATCTGCGGGTTCACAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

543

Amino Acids

60.7

Weight (kDa)

9.53

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 79 - 325 4.3e-29 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 931
AccB1I GGYRCC 1 cut(s) 931
AccB7I CCANNNNNTGG 1 cut(s) 1425
AccBSI CCGCTC 1 cut(s) 1020
AccIII TCCGGA 1 cut(s) 758
AciI CCGC 4 cut(s) 643, 1018, 1103, 1617
AclI AACGTT 1 cut(s) 1027
AclWI GGATC 4 cut(s) 647, 660, 1512, 1559
AcsI RAATTY 6 cut(s) 378, 407, 853, 1169, 1214, 1309
AfaI GTAC 3 cut(s) 178, 519, 966
AfiI CCNNNNNNNGG 5 cut(s) 635, 649, 1425, 1455, 1461
AflII CTTAAG 1 cut(s) 959
AgeI ACCGGT 1 cut(s) 1586
AgsI TTSAA 9 cut(s) 134, 297, 463, 477, 853, 972, 1073, 1168, 1324
AjnI CCWGG 5 cut(s) 79, 645, 810, 982, 1454
AleI CACNNNNGTG 2 cut(s) 689, 839
AluBI AGCT 8 cut(s) 50, 337, 356, 452, 515, 1088, 1148, 1433
AluI AGCT 8 cut(s) 50, 337, 356, 452, 515, 1088, 1148, 1433
Alw26I GTCTC 2 cut(s) 760, 903
AlwI GGATC 4 cut(s) 647, 660, 1512, 1559
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 2 cut(s) 633, 1572
Aor13HI TCCGGA 1 cut(s) 758
AoxI GGCC 5 cut(s) 399, 480, 813, 1015, 1294
ApeKI GCWGC 2 cut(s) 356, 435
ApoI RAATTY 6 cut(s) 378, 407, 853, 1169, 1214, 1309
AsiGI ACCGGT 1 cut(s) 1586
Asp700I GAANNNNTTC 3 cut(s) 129, 411, 1163
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 5 cut(s) 65, 83, 400, 761, 1015
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 5 cut(s) 407, 458, 1089, 1247, 1576
AsuII TTCGAA 1 cut(s) 1306
AvaI CYCGRG 2 cut(s) 633, 1572
AvaII GGWCC 3 cut(s) 65, 83, 761
BamHI GGATCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 931
BanII GRGCYC 1 cut(s) 1387
BbsI GAAGAC 1 cut(s) 1522
BbvI GCAGC 2 cut(s) 343, 447
BccI CCATC 2 cut(s) 83, 426
BceAI ACGGC 1 cut(s) 1472
BciT130I CCWGG 5 cut(s) 81, 647, 812, 984, 1456
BciVI GTATCC 1 cut(s) 650
BclI TGATCA 2 cut(s) 597, 1119
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 760, 903
BfaI CTAG 2 cut(s) 873, 1467
BfrI CTTAAG 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 931
BfuI GTATCC 1 cut(s) 650
BisI GCNGC 3 cut(s) 357, 436, 1018
BlsI GCNGC 3 cut(s) 358, 437, 1019
Bme1390I CCNGG 6 cut(s) 81, 188, 647, 812, 984, 1456
Bme18I GGWCC 3 cut(s) 65, 83, 761
BmeT110I CYCGRG 2 cut(s) 633, 1572
BmgT120I GGNCC 5 cut(s) 65, 83, 400, 761, 1015
BmiI GGNNCC 8 cut(s) 66, 654, 738, 933, 988, 1016, 1228, 1591
BmrFI CCNGG 6 cut(s) 81, 188, 647, 812, 984, 1456
BmrI ACTGGG 2 cut(s) 539, 556
BmsI GCATC 3 cut(s) 111, 1042, 1530
BmuI ACTGGG 2 cut(s) 539, 556
BpiI GAAGAC 1 cut(s) 1522
Bpu14I TTCGAA 1 cut(s) 1306
BpuEI CTTGAG 2 cut(s) 1371, 1379
BpuMI CCSGG 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 79, 646, 1297, 1455
BsaWI WCCGGW 2 cut(s) 758, 1586
Bsc4I CCNNNNNNNGG 5 cut(s) 635, 649, 1425, 1455, 1461
Bse118I RCCGGY 2 cut(s) 1099, 1586
Bse1I ACTGG 5 cut(s) 178, 373, 534, 551, 1256
Bse3DI GCAATG 1 cut(s) 1530
BseAI TCCGGA 1 cut(s) 758
BseBI CCWGG 5 cut(s) 81, 647, 812, 984, 1456
BseDI CCNNGG 4 cut(s) 79, 646, 1297, 1455
BseLI CCNNNNNNNGG 5 cut(s) 635, 649, 1425, 1455, 1461
BseMI GCAATG 1 cut(s) 1530
BseMII CTCAG 2 cut(s) 810, 918
BseNI ACTGG 5 cut(s) 178, 373, 534, 551, 1256
BseXI GCAGC 2 cut(s) 343, 447
BseYI CCCAGC 1 cut(s) 936
BshFI GGCC 5 cut(s) 401, 482, 815, 1017, 1296
BshNI GGYRCC 1 cut(s) 931
BshTI ACCGGT 1 cut(s) 1586
BsiHKCI CYCGRG 2 cut(s) 633, 1572
BsiSI CCGG 4 cut(s) 188, 759, 1100, 1587
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 5 cut(s) 635, 649, 1425, 1455, 1461
BsmAI GTCTC 2 cut(s) 760, 903
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 2 cut(s) 10, 1245
BsnI GGCC 5 cut(s) 401, 482, 815, 1017, 1296
BsoBI CYCGRG 2 cut(s) 633, 1572
Bsp119I TTCGAA 1 cut(s) 1306
Bsp1286I GDGCHC 1 cut(s) 1387
Bsp13I TCCGGA 1 cut(s) 758
Bsp143I GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
BspACI CCGC 4 cut(s) 643, 1018, 1103, 1617
BspANI GGCC 5 cut(s) 401, 482, 815, 1017, 1296
BspCNI CTCAG 2 cut(s) 811, 919
BspEI TCCGGA 1 cut(s) 758
BspLI GGNNCC 8 cut(s) 66, 654, 738, 933, 988, 1016, 1228, 1591
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 4 cut(s) 647, 660, 1512, 1559
BspT104I TTCGAA 1 cut(s) 1306
BspT107I GGYRCC 1 cut(s) 931
BspTI CTTAAG 1 cut(s) 959
BsrBI CCGCTC 1 cut(s) 1020
BsrDI GCAATG 1 cut(s) 1530
BsrFI RCCGGY 2 cut(s) 1099, 1586
BsrI ACTGG 5 cut(s) 178, 373, 534, 551, 1256
BssAI RCCGGY 2 cut(s) 1099, 1586
BssECI CCNNGG 4 cut(s) 79, 646, 1297, 1455
BssMI GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
BssT1I CCWWGG 1 cut(s) 1297
Bst2UI CCWGG 5 cut(s) 81, 647, 812, 984, 1456
Bst4CI ACNGT 2 cut(s) 130, 612
Bst6I CTCTTC 1 cut(s) 1456
BstAFI CTTAAG 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 1306
BstC8I GCNNGC 2 cut(s) 1352, 1480
BstDEI CTNAG 3 cut(s) 222, 819, 927
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 7 cut(s) 544, 600, 655, 885, 1122, 1507, 1554
BstMAI GTCTC 2 cut(s) 760, 903
BstMBI GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
BstMWI GCNNNNNNNGC 3 cut(s) 1145, 1382, 1484
BstNI CCWGG 5 cut(s) 81, 647, 812, 984, 1456
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 6 cut(s) 79, 186, 645, 810, 982, 1454
BstV1I GCAGC 2 cut(s) 343, 447
BstV2I GAAGAC 1 cut(s) 1522
BstX2I RGATCY 2 cut(s) 652, 1504
BstYI RGATCY 2 cut(s) 652, 1504
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 5 cut(s) 401, 482, 815, 1017, 1296
BtsIMutI CAGTG 5 cut(s) 544, 684, 1188, 1249, 1561
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 2 cut(s) 1352, 1480
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 235
Cfr10I RCCGGY 2 cut(s) 1099, 1586
Cfr13I GGNCC 5 cut(s) 65, 83, 400, 761, 1015
Csp6I GTAC 3 cut(s) 177, 518, 965
CspAI ACCGGT 1 cut(s) 1586
CviAII CATG 4 cut(s) 242, 404, 1156, 1544
CviQI GTAC 3 cut(s) 177, 518, 965
DdeI CTNAG 3 cut(s) 222, 819, 927
DpnI GATC 7 cut(s) 543, 599, 654, 884, 1121, 1506, 1553
DpnII GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
Eam1104I CTCTTC 1 cut(s) 1456
EarI CTCTTC 1 cut(s) 1456
EciI GGCGGA 1 cut(s) 632
Eco130I CCWWGG 1 cut(s) 1297
Eco147I AGGCCT 2 cut(s) 482, 815
Eco24I GRGCYC 1 cut(s) 1387
Eco47I GGWCC 3 cut(s) 65, 83, 761
Eco88I CYCGRG 2 cut(s) 633, 1572
EcoO109I RGGNCCY 2 cut(s) 65, 83
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 5 cut(s) 79, 645, 810, 982, 1454
EcoT14I CCWWGG 1 cut(s) 1297
EcoT22I ATGCAT 1 cut(s) 1545
EcoT38I GRGCYC 1 cut(s) 1387
ErhI CCWWGG 1 cut(s) 1297
FaeI CATG 4 cut(s) 245, 407, 1159, 1547
FaqI GGGAC 1 cut(s) 78
FatI CATG 4 cut(s) 241, 403, 1155, 1543
FauI CCCGC 2 cut(s) 1096, 1610
FbaI TGATCA 2 cut(s) 597, 1119
Fnu4HI GCNGC 3 cut(s) 357, 436, 1018
FriOI GRGCYC 1 cut(s) 1387
Fsp4HI GCNGC 3 cut(s) 357, 436, 1018
FspBI CTAG 2 cut(s) 873, 1467
GlaI GCGC 1 cut(s) 234
GluI GCNGC 3 cut(s) 357, 436, 1018
GsaI CCCAGC 1 cut(s) 940
HaeIII GGCC 5 cut(s) 401, 482, 815, 1017, 1296
HapII CCGG 4 cut(s) 188, 759, 1100, 1587
HhaI GCGC 1 cut(s) 235
Hin1II CATG 4 cut(s) 245, 407, 1159, 1547
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 450, 1086
HinfI GANTC 6 cut(s) 310, 794, 1069, 1141, 1560, 1566
HpaII CCGG 4 cut(s) 188, 759, 1100, 1587
HphI GGTGA 5 cut(s) 407, 458, 1089, 1247, 1576
Hpy166II GTNNAC 2 cut(s) 1001, 1623
Hpy188I TCNGA 6 cut(s) 22, 346, 597, 820, 928, 1565
Hpy188III TCNNGA 4 cut(s) 86, 383, 759, 1049
Hpy8I GTNNAC 2 cut(s) 1001, 1623
HpyAV CCTTC 5 cut(s) 1102, 1380, 1432, 1542, 1613
HpyCH4III ACNGT 2 cut(s) 130, 612
HpyCH4IV ACGT 1 cut(s) 1027
HpyCH4V TGCA 6 cut(s) 539, 745, 1065, 1188, 1211, 1543
HpyF10VI GCNNNNNNNGC 3 cut(s) 1145, 1382, 1484
HpyF3I CTNAG 3 cut(s) 222, 819, 927
HpySE526I ACGT 1 cut(s) 1027
Hsp92II CATG 4 cut(s) 245, 407, 1159, 1547
HspAI GCGC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 758
Ksp22I TGATCA 2 cut(s) 597, 1119
Kzo9I GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
LmnI GCTCC 2 cut(s) 189, 1264
Lsp1109I GCAGC 2 cut(s) 343, 447
LweI GCATC 3 cut(s) 111, 1042, 1530
MaeI CTAG 2 cut(s) 873, 1467
MaeII ACGT 1 cut(s) 1027
MaeIII GTNAC 5 cut(s) 25, 364, 682, 1095, 1582
MalI GATC 7 cut(s) 543, 599, 654, 884, 1121, 1506, 1553
MbiI CCGCTC 1 cut(s) 1020
MboI GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
MflI RGATCY 2 cut(s) 652, 1504
MhlI GDGCHC 1 cut(s) 1387
MlyI GAGTC 1 cut(s) 788
Mph1103I ATGCAT 1 cut(s) 1545
MroI TCCGGA 1 cut(s) 758
MroXI GAANNNNTTC 3 cut(s) 129, 411, 1163
MseI TTAA 5 cut(s) 72, 215, 423, 960, 1630
MslI CAYNNNNRTG 2 cut(s) 689, 839
MspCI CTTAAG 1 cut(s) 959
MspI CCGG 4 cut(s) 188, 759, 1100, 1587
MspR9I CCNGG 6 cut(s) 81, 188, 647, 812, 984, 1456
Mva1269I GAATGC 2 cut(s) 10, 1245
MvaI CCWGG 5 cut(s) 81, 647, 812, 984, 1456
MwoI GCNNNNNNNGC 3 cut(s) 1145, 1382, 1484
NciI CCSGG 1 cut(s) 188
NdeII GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
NlaIII CATG 4 cut(s) 245, 407, 1159, 1547
NlaIV GGNNCC 8 cut(s) 66, 654, 738, 933, 988, 1016, 1228, 1591
NmuCI GTSAC 3 cut(s) 682, 1095, 1582
NsiI ATGCAT 1 cut(s) 1545
NspI RCATGY 1 cut(s) 245
NspV TTCGAA 1 cut(s) 1306
OliI CACNNNNGTG 2 cut(s) 689, 839
PaeR7I CTCGAG 2 cut(s) 633, 1572
PceI AGGCCT 2 cut(s) 482, 815
PctI GAATGC 2 cut(s) 10, 1245
PdmI GAANNNNTTC 3 cut(s) 129, 411, 1163
PfeI GAWTC 5 cut(s) 310, 1069, 1141, 1560, 1566
PflMI CCANNNNNTGG 1 cut(s) 1425
PfoI TCCNGGA 1 cut(s) 186
PinAI ACCGGT 1 cut(s) 1586
PkrI GCNGC 3 cut(s) 358, 437, 1019
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
PpuMI RGGWCCY 2 cut(s) 65, 83
PsiI TTATAA 1 cut(s) 285
Psp1406I AACGTT 1 cut(s) 1027
Psp5II RGGWCCY 2 cut(s) 65, 83
Psp6I CCWGG 5 cut(s) 79, 645, 810, 982, 1454
PspFI CCCAGC 1 cut(s) 936
PspGI CCWGG 5 cut(s) 79, 645, 810, 982, 1454
PspN4I GGNNCC 8 cut(s) 66, 654, 738, 933, 988, 1016, 1228, 1591
PspPI GGNCC 5 cut(s) 65, 83, 400, 761, 1015
PspPPI RGGWCCY 2 cut(s) 65, 83
PspXI VCTCGAGB 2 cut(s) 633, 1572
PsrI GAACNNNNNNTAC 2 cut(s) 193, 225
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 652, 1504
RsaI GTAC 3 cut(s) 178, 519, 966
RsaNI GTAC 3 cut(s) 177, 518, 965
RseI CAYNNNNRTG 2 cut(s) 689, 839
SaqAI TTAA 5 cut(s) 72, 215, 423, 960, 1630
SatI GCNGC 3 cut(s) 357, 436, 1018
Sau3AI GATC 7 cut(s) 541, 597, 652, 882, 1119, 1504, 1551
Sau96I GGNCC 5 cut(s) 65, 83, 400, 761, 1015
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 6 cut(s) 81, 188, 647, 812, 984, 1456
SduI GDGCHC 1 cut(s) 1387
SfaNI GCATC 3 cut(s) 111, 1042, 1530
Sfr274I CTCGAG 2 cut(s) 633, 1572
SfuI TTCGAA 1 cut(s) 1306
SgrAI CRCCGGYG 1 cut(s) 1099
SinI GGWCC 3 cut(s) 65, 83, 761
SlaI CTCGAG 2 cut(s) 633, 1572
SmiMI CAYNNNNRTG 2 cut(s) 689, 839
SmlI CTYRAG 5 cut(s) 633, 959, 1386, 1394, 1572
SmoI CTYRAG 5 cut(s) 633, 959, 1386, 1394, 1572
SseBI AGGCCT 2 cut(s) 482, 815
SsiI CCGC 4 cut(s) 643, 1018, 1103, 1617
SspMI CTAG 2 cut(s) 873, 1467
StuI AGGCCT 2 cut(s) 482, 815
StyD4I CCNGG 6 cut(s) 79, 186, 645, 810, 982, 1454
StyI CCWWGG 1 cut(s) 1297
TaaI ACNGT 2 cut(s) 130, 612
TaiI ACGT 1 cut(s) 1030
TaqI TCGA 4 cut(s) 634, 1280, 1306, 1573
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 1020
TfiI GAWTC 5 cut(s) 310, 1069, 1141, 1560, 1566
Tru1I TTAA 5 cut(s) 72, 215, 423, 960, 1630
Tru9I TTAA 5 cut(s) 72, 215, 423, 960, 1630
TscAI CASTG 5 cut(s) 551, 691, 1195, 1256, 1561
TseFI GTSAC 3 cut(s) 682, 1095, 1582
TseI GCWGC 2 cut(s) 356, 435
Tsp45I GTSAC 3 cut(s) 682, 1095, 1582
TspDTI ATGAA 6 cut(s) 17, 158, 209, 420, 578, 1172
TspGWI ACGGA 1 cut(s) 1276
TspRI CASTG 5 cut(s) 551, 691, 1195, 1256, 1561
Van91I CCANNNNNTGG 1 cut(s) 1425
Vha464I CTTAAG 1 cut(s) 959
VpaK11BI GGWCC 3 cut(s) 65, 83, 761
XapI RAATTY 6 cut(s) 378, 407, 853, 1169, 1214, 1309
XceI RCATGY 1 cut(s) 245
XhoI CTCGAG 2 cut(s) 633, 1572
XmnI GAANNNNTTC 3 cut(s) 129, 411, 1163
XspI CTAG 2 cut(s) 873, 1467
Zsp2I ATGCAT 1 cut(s) 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.