RchiOBHm_Chr5g0040851

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000194
Physical Location & Seq
Reverse (-)
35465639 .. 35468350
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31932

Sequence Viewer

Length: 1965 bp
ATGGTGACCTTGAGGTTACTGATTCTCCTCGTCCTCCCAAGAGGCATAGGCGTACCAAGCTTCAGATGGCGGAGGCACGGGCTGAAAAGGAAGCCAGGTTTGCCTACTGTTTGGGGCAGATCGAAGCGTGCAGCTTTGGGTTATGTTCGAGATGCTGTTCTTAGGAAAATTCAAGGTTGGAAACAAGCTTCATTATCTCCGGCAGGAAAGGAAATCATGATAAAGGCGGTGGCAATGGCACTTCCTGCCTATCCAATGTCAATTTTCAAGTTTCCAAAATCCCTTTGTGTTCAGATTAATAATGCTTTGGCAAAGTTTTGGTGGGGTACAAATGGTGACAAGGGTATTCACTGGAAGCATTGGGATCTTTTGTGCAATCCGAAATCTGTGGGAGGTCTTGGTTTTCGTAGCTTACAGGACTTCAATTTAGCTTTGTTAGCTAAGCAGGGTTGGCGTCTGCTTCAAAATCCTTCTGCAAAATGGGCTCAGGTCCTGAAATATAGGTATTTCCCAAATACCTCCTTCATGGAGGCCAAACAGGGTCCTTCATCTTCCTGGGTGTGGGCGAGTCTGTTGGCTGGTAGAGATGTGTTATTTAAAGGCTCATTATGGAGAGTGGGTAATGGTCACTCTATACATTTATGGAATGACAAATGGATCCCAAACACTCCCAGCTTCAGTACTAGGTTCCCTTCTCTCCTGCCTGTCTCACAATCTGTAAACACCCTTTTAGATTTCTCTACCCTCACCTGGAATTTGGAGTCCCTTCAACATGTGCTTACTCCGGGTGATTCTTCTGTGATTCAGGCCATTCCTATTTTCAGTGAGTTGGTTGATGATAAACTCGTGTGGCCTCATGTTATGAATGGTGAGTATTCGGTGAGGTCGGGCTATCATGTCTCTTTTAACTCAAATATTATTGCTTCAGCTCCATCTCCACATTCCTCTCATGTAGTCCCTCGATCAGTTTGGACTTTGGTTTGGACTATTCGGAGTCTTCCAAAAATAAGGATTTTCCTATGGAGAGTTGTGTCTAATGTGTTAGCAACCAAACAGAATTTGTTTAGAAGACACCTGGGGCGCTCACCTCTCTGTCCTTTTTGCTCAGAATTCCCAGAATCAGTGGAACATCTTCTTCTCTCTTGTCCTTGGGTTGTGCAAACCTGGTTTTTGCACCCACTGGGATATCGGATTCCCTTGCAATCCCTTACTTCTTTTGATCAGTGGTTAAGTGCTCTACTTATTCCTCTTCATTCTGAGTTGTCAAGATCTGTGGTGCTTACTCATATCAGCTTTCTTCTTTGGCACATTTGGAAGCATCGGTGTGATTGCATTTTCAATTACAAGGTTCCTAATCCGACTTTGGTGGCTACGAGGGCTTTTGTGGCTTCTTCAGAGTTTTTAACATCTCGTACTCTGTCTCTTACTTGCAATAGGCCTCCCCCTCCTCCCCCTCCTCCTCCTCCACCTTGGACTCCTCCTTGTTTCGATTTTGTTAAGATAAATACAGATGCTTCTTGGGATAAACAATCTCTGCAGGCTGGTTTGGCAGTGATAGTTCGAAATAGTTCTGGTGAGCTCCTTCATGGCAAGACGAGGATTGTTAGGGCTTCTTCGATCCTTCTTGCTGAAACTTTGGCCCTTTATCAAGCCGTGTTGCTGGCCTTGTGTTTAAATTTTCAAAAAGTTATTTTTGAAATGGATTCCTTTCATCTTGTGCATCTCCTGAATGATTCCCACTTGCTACCGGATTGGATCATATCTCCGCTGGTGCATTATATCCGTGAGATCACAGTTGTTATTCCTTACGTTAGCTGGGTTTGGACCAGCAGGAACGCCAATCAAGTTGCTGATCATATGGCTTCTTTGGCTAGCAGGAGGATGGGCCCTTCTGATTGGGTTAATCATCCTCCTTCTACCTTCACCCGTATTCTCCTTTATGATGCTGGACATGCTCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

654

Amino Acids

74.12

Weight (kDa)

10.02

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 292 - 389 5.8e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 502 - 623 4e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 70, 227, 1766
AclWI GGATC 5 cut(s) 372, 652, 665, 1612, 1763
AcsI RAATTY 5 cut(s) 168, 754, 1057, 1109, 1675
AcuI CTGAAG 4 cut(s) 46, 661, 909, 1377
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 4 cut(s) 54, 328, 682, 1414
AfiI CCNNNNNNNGG 2 cut(s) 561, 750
AflIII ACRYGT 1 cut(s) 772
AgsI TTSAA 8 cut(s) 173, 268, 424, 464, 770, 1339, 1682, 1697
AjnI CCWGG 5 cut(s) 94, 554, 749, 1074, 1163
Alw21I GWGCWC 2 cut(s) 1237, 1581
Alw26I GTCTC 3 cut(s) 712, 904, 1425
AlwI GGATC 5 cut(s) 372, 652, 665, 1612, 1763
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 7 cut(s) 531, 807, 851, 1436, 1638, 1662, 1885
ApaI GGGCCC 1 cut(s) 1889
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 5 cut(s) 168, 754, 1057, 1109, 1675
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 2 cut(s) 1131, 1567
AspLEI GCGC 1 cut(s) 1083
AspS9I GGNCC 6 cut(s) 490, 542, 1639, 1824, 1885, 1886
AsuC2I CCSGG 1 cut(s) 786
AsuHPI GGTGA 9 cut(s) 16, 347, 739, 800, 881, 892, 1077, 1586, 1915
AsuII TTCGAA 1 cut(s) 1561
AsuNHI GCTAGC 1 cut(s) 1871
AvaII GGWCC 3 cut(s) 490, 542, 1824
BaeGI GKGCMC 1 cut(s) 1889
BamHI GGATCC 1 cut(s) 657
BanII GRGCYC 3 cut(s) 487, 1581, 1889
BauI CACGAG 1 cut(s) 845
BbsI GAAGAC 2 cut(s) 989, 1075
Bbv12I GWGCWC 2 cut(s) 1237, 1581
BbvI GCAGC 1 cut(s) 143
BccI CCATC 3 cut(s) 60, 940, 1876
BceAI ACGGC 1 cut(s) 1637
BciT130I CCWGG 5 cut(s) 96, 556, 751, 1076, 1165
BclI TGATCA 2 cut(s) 1219, 1852
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 3 cut(s) 712, 904, 1425
BfaI CTAG 2 cut(s) 684, 1872
BfmI CTRYAG 1 cut(s) 1535
BfoI RGCGCY 1 cut(s) 1084
BglII AGATCT 1 cut(s) 1268
BisI GCNGC 1 cut(s) 132
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 133
BmcAI AGTACT 1 cut(s) 682
Bme1390I CCNGG 6 cut(s) 96, 556, 751, 786, 1076, 1165
Bme18I GGWCC 3 cut(s) 490, 542, 1824
BmgT120I GGNCC 6 cut(s) 490, 542, 1639, 1824, 1885, 1886
BmiI GGNNCC 5 cut(s) 543, 659, 689, 1350, 1887
BmrFI CCNGG 6 cut(s) 96, 556, 751, 786, 1076, 1165
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 5 cut(s) 142, 1327, 1501, 1729, 1933
BmtI GCTAGC 1 cut(s) 1875
BmuI ACTGGG 1 cut(s) 1190
BpiI GAAGAC 2 cut(s) 989, 1075
Bpu10I CCTNAGC 1 cut(s) 486
Bpu1102I GCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 1561
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 4 cut(s) 555, 1075, 1148, 1469
BsaWI WCCGGW 1 cut(s) 1747
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 604, 634
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 750
Bse1I ACTGG 2 cut(s) 356, 1185
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 5 cut(s) 96, 556, 751, 1076, 1165
BseDI CCNNGG 4 cut(s) 555, 1075, 1148, 1469
BseGI GGATG 2 cut(s) 1887, 1906
BseLI CCNNNNNNNGG 2 cut(s) 561, 750
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 3 cut(s) 500, 1119, 1248
BseNI ACTGG 2 cut(s) 356, 1185
BseRI GAGGAG 6 cut(s) 17, 1437, 1446, 1449, 1452, 1467
BseSI GKGCMC 1 cut(s) 1889
BseXI GCAGC 1 cut(s) 143
BseYI CCCAGC 2 cut(s) 671, 1815
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 7 cut(s) 533, 809, 853, 1438, 1640, 1664, 1887
BsiHKAI GWGCWC 2 cut(s) 1237, 1581
BsiSI CCGG 3 cut(s) 200, 785, 1748
BslFI GGGAC 2 cut(s) 748, 941
BslI CCNNNNNNNGG 2 cut(s) 561, 750
BsmAI GTCTC 3 cut(s) 712, 904, 1425
BsmFI GGGAC 2 cut(s) 748, 941
BsnI GGCC 7 cut(s) 533, 809, 853, 1438, 1640, 1664, 1887
Bsp119I TTCGAA 1 cut(s) 1561
Bsp120I GGGCCC 1 cut(s) 1885
Bsp1286I GDGCHC 4 cut(s) 487, 1237, 1581, 1889
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 3 cut(s) 70, 227, 1766
BspANI GGCC 7 cut(s) 533, 809, 853, 1438, 1640, 1664, 1887
BspCNI CTCAG 3 cut(s) 499, 1118, 1249
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 5 cut(s) 543, 659, 689, 1350, 1887
BspMAI CTGCAG 1 cut(s) 1539
BspOI GCTAGC 1 cut(s) 1875
BspPI GGATC 5 cut(s) 372, 652, 665, 1612, 1763
BspT104I TTCGAA 1 cut(s) 1561
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 2 cut(s) 356, 1185
BssECI CCNNGG 4 cut(s) 555, 1075, 1148, 1469
BssNI GRCGYC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 845
BssT1I CCWWGG 2 cut(s) 1148, 1469
Bst2BI CACGAG 1 cut(s) 845
Bst2UI CCWGG 5 cut(s) 96, 556, 751, 1076, 1165
Bst4CI ACNGT 2 cut(s) 109, 1795
Bst6I CTCTTC 1 cut(s) 1254
BstACI GRCGYC 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 245
BstBI TTCGAA 1 cut(s) 1561
BstC8I GCNNGC 4 cut(s) 129, 1539, 1662, 1873
BstDEI CTNAG 5 cut(s) 161, 441, 486, 1105, 1257
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 2 cut(s) 1887, 1906
BstH2I RGCGCY 1 cut(s) 1084
BstHHI GCGC 1 cut(s) 1083
BstMAI GTCTC 3 cut(s) 712, 904, 1425
BstNI CCWGG 5 cut(s) 96, 556, 751, 1076, 1165
BstNSI RCATGY 2 cut(s) 776, 1955
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 6 cut(s) 94, 554, 749, 784, 1074, 1163
BstSFI CTRYAG 1 cut(s) 1535
BstSLI GKGCMC 1 cut(s) 1889
BstV1I GCAGC 1 cut(s) 143
BstV2I GAAGAC 2 cut(s) 989, 1075
BstX2I RGATCY 3 cut(s) 364, 657, 1268
BstYI RGATCY 3 cut(s) 364, 657, 1268
BsuRI GGCC 7 cut(s) 533, 809, 853, 1438, 1640, 1664, 1887
BtsCI GGATG 2 cut(s) 1887, 1906
BtsI GCAGTG 1 cut(s) 1557
BtsIMutI CAGTG 6 cut(s) 349, 829, 1128, 1178, 1229, 1557
Cac8I GCNNGC 4 cut(s) 129, 1539, 1662, 1873
CaiI CAGNNNCTG 1 cut(s) 493
CciI TCATGA 1 cut(s) 216
CfoI GCGC 1 cut(s) 1083
Cfr13I GGNCC 6 cut(s) 490, 542, 1639, 1824, 1885, 1886
CseI GACGC 1 cut(s) 443
CsiI ACCWGGT 1 cut(s) 1163
Csp6I GTAC 4 cut(s) 53, 327, 681, 1413
CspCI CAANNNNNGTGG 2 cut(s) 1254, 1289
CviAII CATG 8 cut(s) 217, 526, 773, 857, 896, 950, 1586, 1952
CviQI GTAC 4 cut(s) 53, 327, 681, 1413
DdeI CTNAG 5 cut(s) 161, 441, 486, 1105, 1257
DraI TTTAAA 2 cut(s) 598, 1674
Eam1104I CTCTTC 1 cut(s) 1254
EarI CTCTTC 1 cut(s) 1254
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 1579
Eco130I CCWWGG 2 cut(s) 1148, 1469
Eco147I AGGCCT 1 cut(s) 1438
Eco24I GRGCYC 3 cut(s) 487, 1581, 1889
Eco32I GATATC 1 cut(s) 1187
Eco47I GGWCC 3 cut(s) 490, 542, 1824
Eco53kI GAGCTC 1 cut(s) 1579
Eco57I CTGAAG 4 cut(s) 46, 661, 909, 1377
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 1579
EcoO109I RGGNCCY 3 cut(s) 490, 542, 1886
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 1109
EcoRII CCWGG 5 cut(s) 94, 554, 749, 1074, 1163
EcoRV GATATC 1 cut(s) 1187
EcoT14I CCWWGG 2 cut(s) 1148, 1469
EcoT38I GRGCYC 3 cut(s) 487, 1581, 1889
ErhI CCWWGG 2 cut(s) 1148, 1469
FaeI CATG 8 cut(s) 220, 529, 776, 860, 899, 953, 1589, 1955
FaqI GGGAC 2 cut(s) 748, 941
FatI CATG 8 cut(s) 216, 525, 772, 856, 895, 949, 1585, 1951
FauNDI CATATG 1 cut(s) 1857
FbaI TGATCA 2 cut(s) 1219, 1852
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 2 cut(s) 1893, 1894
FriOI GRGCYC 3 cut(s) 487, 1581, 1889
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 2 cut(s) 684, 1872
GlaI GCGC 1 cut(s) 1082
GluI GCNGC 1 cut(s) 132
GsaI CCCAGC 2 cut(s) 675, 1819
HaeII RGCGCY 1 cut(s) 1084
HaeIII GGCC 7 cut(s) 533, 809, 853, 1438, 1640, 1664, 1887
HapII CCGG 3 cut(s) 200, 785, 1748
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 1083
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 8 cut(s) 220, 529, 776, 860, 899, 953, 1589, 1955
Hin6I GCGC 1 cut(s) 1081
HinP1I GCGC 1 cut(s) 1081
HindIII AAGCTT 2 cut(s) 58, 186
HpaII CCGG 3 cut(s) 200, 785, 1748
HphI GGTGA 9 cut(s) 16, 347, 739, 800, 881, 892, 1077, 1586, 1915
Hpy166II GTNNAC 1 cut(s) 721
Hpy188III TCNNGA 5 cut(s) 149, 217, 493, 1266, 1726
Hpy8I GTNNAC 1 cut(s) 721
HpyCH4III ACNGT 2 cut(s) 109, 1795
HpyCH4IV ACGT 1 cut(s) 1809
HpyF3I CTNAG 5 cut(s) 161, 441, 486, 1105, 1257
HpySE526I ACGT 1 cut(s) 1809
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 8 cut(s) 220, 529, 776, 860, 899, 953, 1589, 1955
HspAI GCGC 1 cut(s) 1081
Ksp22I TGATCA 2 cut(s) 1219, 1852
LmnI GCTCC 3 cut(s) 934, 1584, 1960
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 5 cut(s) 142, 1327, 1501, 1729, 1933
MabI ACCWGGT 1 cut(s) 1163
MaeI CTAG 2 cut(s) 684, 1872
MaeII ACGT 1 cut(s) 1809
MaeIII GTNAC 4 cut(s) 4, 15, 335, 626
MflI RGATCY 3 cut(s) 364, 657, 1268
MhlI GDGCHC 4 cut(s) 487, 1237, 1581, 1889
MluCI AATT 8 cut(s) 168, 261, 424, 754, 1057, 1109, 1339, 1675
MlyI GAGTC 4 cut(s) 577, 770, 1003, 1468
MmeI TCCRAC 2 cut(s) 158, 1382
MroXI GAANNNNTTC 2 cut(s) 1131, 1567
MseI TTAA 9 cut(s) 297, 597, 906, 1229, 1403, 1497, 1673, 1902, 1963
MslI CAYNNNNRTG 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 1768
MspI CCGG 3 cut(s) 200, 785, 1748
MspR9I CCNGG 6 cut(s) 96, 556, 751, 786, 1076, 1165
MvaI CCWGG 5 cut(s) 96, 556, 751, 1076, 1165
NciI CCSGG 1 cut(s) 786
NdeI CATATG 1 cut(s) 1857
NheI GCTAGC 1 cut(s) 1871
NlaIII CATG 8 cut(s) 220, 529, 776, 860, 899, 953, 1589, 1955
NlaIV GGNNCC 5 cut(s) 543, 659, 689, 1350, 1887
NmuCI GTSAC 3 cut(s) 4, 335, 626
NspI RCATGY 2 cut(s) 776, 1955
NspV TTCGAA 1 cut(s) 1561
PagI TCATGA 1 cut(s) 216
PceI AGGCCT 1 cut(s) 1438
PciI ACATGT 1 cut(s) 772
PdmI GAANNNNTTC 2 cut(s) 1131, 1567
PfeI GAWTC 7 cut(s) 22, 791, 802, 1118, 1192, 1703, 1733
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 4 cut(s) 576, 769, 1002, 1468
PpsI GAGTC 4 cut(s) 576, 769, 1002, 1468
PpuMI RGGWCCY 2 cut(s) 490, 542
PscI ACATGT 1 cut(s) 772
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 1581
Psp5II RGGWCCY 2 cut(s) 490, 542
Psp6I CCWGG 5 cut(s) 94, 554, 749, 1074, 1163
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 2 cut(s) 671, 1815
PspGI CCWGG 5 cut(s) 94, 554, 749, 1074, 1163
PspN4I GGNNCC 5 cut(s) 543, 659, 689, 1350, 1887
PspOMI GGGCCC 1 cut(s) 1885
PspPI GGNCC 6 cut(s) 490, 542, 1639, 1824, 1885, 1886
PspPPI RGGWCCY 2 cut(s) 490, 542
PstI CTGCAG 1 cut(s) 1539
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 3 cut(s) 364, 657, 1268
RsaI GTAC 4 cut(s) 54, 328, 682, 1414
RsaNI GTAC 4 cut(s) 53, 327, 681, 1413
RseI CAYNNNNRTG 1 cut(s) 1323
SacI GAGCTC 1 cut(s) 1581
SaqAI TTAA 9 cut(s) 297, 597, 906, 1229, 1403, 1497, 1673, 1902, 1963
SatI GCNGC 1 cut(s) 132
Sau96I GGNCC 6 cut(s) 490, 542, 1639, 1824, 1885, 1886
ScaI AGTACT 1 cut(s) 682
SchI GAGTC 4 cut(s) 577, 770, 1003, 1468
ScrFI CCNGG 6 cut(s) 96, 556, 751, 786, 1076, 1165
SduI GDGCHC 4 cut(s) 487, 1237, 1581, 1889
SexAI ACCWGGT 1 cut(s) 1163
SfaNI GCATC 5 cut(s) 142, 1327, 1501, 1729, 1933
SfcI CTRYAG 1 cut(s) 1535
SfuI TTCGAA 1 cut(s) 1561
SinI GGWCC 3 cut(s) 490, 542, 1824
SmiMI CAYNNNNRTG 1 cut(s) 1323
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 8 cut(s) 168, 261, 424, 754, 1057, 1109, 1339, 1675
SseBI AGGCCT 1 cut(s) 1438
SsiI CCGC 3 cut(s) 70, 227, 1766
SspI AATATT 1 cut(s) 916
SspMI CTAG 2 cut(s) 684, 1872
SstI GAGCTC 1 cut(s) 1581
StuI AGGCCT 1 cut(s) 1438
StyD4I CCNGG 6 cut(s) 94, 554, 749, 784, 1074, 1163
StyI CCWWGG 2 cut(s) 1148, 1469
TaaI ACNGT 2 cut(s) 109, 1795
TaiI ACGT 1 cut(s) 1812
TaqI TCGA 6 cut(s) 122, 148, 961, 1488, 1561, 1616
TasI AATT 8 cut(s) 168, 261, 424, 754, 1057, 1109, 1339, 1675
TatI WGTACW 1 cut(s) 680
TfiI GAWTC 7 cut(s) 22, 791, 802, 1118, 1192, 1703, 1733
Tru1I TTAA 9 cut(s) 297, 597, 906, 1229, 1403, 1497, 1673, 1902, 1963
Tru9I TTAA 9 cut(s) 297, 597, 906, 1229, 1403, 1497, 1673, 1902, 1963
TscAI CASTG 6 cut(s) 356, 829, 1128, 1185, 1229, 1557
TseFI GTSAC 3 cut(s) 4, 335, 626
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 3 cut(s) 4, 335, 626
TspDTI ATGAA 7 cut(s) 180, 514, 537, 878, 1241, 1574, 1700
TspGWI ACGGA 1 cut(s) 1772
TspRI CASTG 6 cut(s) 356, 829, 1128, 1185, 1229, 1557
VpaK11BI GGWCC 3 cut(s) 490, 542, 1824
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 5 cut(s) 168, 754, 1057, 1109, 1675
XceI RCATGY 2 cut(s) 776, 1955
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmnI GAANNNNTTC 2 cut(s) 1131, 1567
XspI CTAG 2 cut(s) 684, 1872
ZrmI AGTACT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.