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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 431, 644 |
| AclWI | GGATC | 1 cut(s) | 552 |
| AcsI | RAATTY | 1 cut(s) | 753 |
| AcyI | GRCGYC | 1 cut(s) | 119 |
| AfaI | GTAC | 1 cut(s) | 673 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 256 |
| AgeI | ACCGGT | 1 cut(s) | 467 |
| AgsI | TTSAA | 2 cut(s) | 152, 765 |
| AluBI | AGCT | 2 cut(s) | 200, 408 |
| AluI | AGCT | 2 cut(s) | 200, 408 |
| Alw21I | GWGCWC | 1 cut(s) | 519 |
| Alw26I | GTCTC | 3 cut(s) | 126, 173, 717 |
| AlwI | GGATC | 1 cut(s) | 552 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 35 |
| AoxI | GGCC | 1 cut(s) | 39 |
| ApeKI | GCWGC | 1 cut(s) | 29 |
| ApoI | RAATTY | 1 cut(s) | 753 |
| AsiGI | ACCGGT | 1 cut(s) | 467 |
| AspA2I | CCTAGG | 1 cut(s) | 590 |
| AspS9I | GGNCC | 2 cut(s) | 443, 535 |
| AsuHPI | GGTGA | 2 cut(s) | 92, 403 |
| AsuNHI | GCTAGC | 1 cut(s) | 404 |
| AvaII | GGWCC | 2 cut(s) | 443, 535 |
| AvrII | CCTAGG | 1 cut(s) | 590 |
| AxyI | CCTNAGG | 1 cut(s) | 539 |
| BaeGI | GKGCMC | 2 cut(s) | 168, 300 |
| BbsI | GAAGAC | 3 cut(s) | 7, 480, 590 |
| Bbv12I | GWGCWC | 1 cut(s) | 519 |
| BbvI | GCAGC | 1 cut(s) | 41 |
| BccI | CCATC | 5 cut(s) | 50, 98, 477, 725, 741 |
| BcoDI | GTCTC | 3 cut(s) | 126, 173, 717 |
| BfaI | CTAG | 2 cut(s) | 405, 591 |
| BfmI | CTRYAG | 2 cut(s) | 75, 195 |
| BisI | GCNGC | 1 cut(s) | 30 |
| BlnI | CCTAGG | 1 cut(s) | 590 |
| BlsI | GCNGC | 1 cut(s) | 31 |
| Bme18I | GGWCC | 2 cut(s) | 443, 535 |
| BmgT120I | GGNCC | 2 cut(s) | 443, 535 |
| BmiI | GGNNCC | 1 cut(s) | 243 |
| BmsI | GCATC | 1 cut(s) | 674 |
| BmtI | GCTAGC | 1 cut(s) | 408 |
| BpiI | GAAGAC | 3 cut(s) | 7, 480, 590 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 47, 79 |
| Bpu10I | CCTNAGC | 1 cut(s) | 267 |
| BsaHI | GRCGYC | 1 cut(s) | 119 |
| BsaI | GGTCTC | 1 cut(s) | 717 |
| BsaJI | CCNNGG | 2 cut(s) | 590, 681 |
| BsaWI | WCCGGW | 1 cut(s) | 467 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 256 |
| Bse118I | RCCGGY | 1 cut(s) | 467 |
| Bse1I | ACTGG | 1 cut(s) | 432 |
| Bse21I | CCTNAGG | 1 cut(s) | 539 |
| BseDI | CCNNGG | 2 cut(s) | 590, 681 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 256 |
| BseMII | CTCAG | 5 cut(s) | 27, 108, 174, 251, 553 |
| BseNI | ACTGG | 1 cut(s) | 432 |
| BseSI | GKGCMC | 2 cut(s) | 168, 300 |
| BseXI | GCAGC | 1 cut(s) | 41 |
| BsgI | GTGCAG | 1 cut(s) | 617 |
| BshFI | GGCC | 1 cut(s) | 41 |
| BshTI | ACCGGT | 1 cut(s) | 467 |
| BsiHKAI | GWGCWC | 1 cut(s) | 519 |
| BsiSI | CCGG | 1 cut(s) | 468 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 256 |
| BsmAI | GTCTC | 3 cut(s) | 126, 173, 717 |
| BsmBI | CGTCTC | 1 cut(s) | 126 |
| BsnI | GGCC | 1 cut(s) | 41 |
| Bso31I | GGTCTC | 1 cut(s) | 717 |
| Bsp1286I | GDGCHC | 3 cut(s) | 168, 300, 519 |
| Bsp143I | GATC | 2 cut(s) | 451, 544 |
| BspACI | CCGC | 2 cut(s) | 431, 644 |
| BspANI | GGCC | 1 cut(s) | 41 |
| BspCNI | CTCAG | 5 cut(s) | 28, 107, 175, 250, 552 |
| BspHI | TCATGA | 1 cut(s) | 282 |
| BspLI | GGNNCC | 1 cut(s) | 243 |
| BspOI | GCTAGC | 1 cut(s) | 408 |
| BspPI | GGATC | 1 cut(s) | 552 |
| BspTNI | GGTCTC | 1 cut(s) | 717 |
| BsrFI | RCCGGY | 1 cut(s) | 467 |
| BsrI | ACTGG | 1 cut(s) | 432 |
| BssAI | RCCGGY | 1 cut(s) | 467 |
| BssECI | CCNNGG | 2 cut(s) | 590, 681 |
| BssMI | GATC | 2 cut(s) | 451, 544 |
| BssNI | GRCGYC | 1 cut(s) | 119 |
| BssT1I | CCWWGG | 2 cut(s) | 590, 681 |
| Bst4CI | ACNGT | 2 cut(s) | 214, 259 |
| BstACI | GRCGYC | 1 cut(s) | 119 |
| BstC8I | GCNNGC | 2 cut(s) | 327, 406 |
| BstDEI | CTNAG | 6 cut(s) | 36, 94, 183, 237, 267, 539 |
| BstKTI | GATC | 2 cut(s) | 454, 547 |
| BstMAI | GTCTC | 3 cut(s) | 126, 173, 717 |
| BstMBI | GATC | 2 cut(s) | 451, 544 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 38, 604, 694 |
| BstSFI | CTRYAG | 2 cut(s) | 75, 195 |
| BstSLI | GKGCMC | 2 cut(s) | 168, 300 |
| BstV1I | GCAGC | 1 cut(s) | 41 |
| BstV2I | GAAGAC | 3 cut(s) | 7, 480, 590 |
| BstX2I | RGATCY | 1 cut(s) | 544 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 421 |
| BstYI | RGATCY | 1 cut(s) | 544 |
| Bsu36I | CCTNAGG | 1 cut(s) | 539 |
| BsuRI | GGCC | 1 cut(s) | 41 |
| BtsIMutI | CAGTG | 2 cut(s) | 425, 491 |
| Cac8I | GCNNGC | 2 cut(s) | 327, 406 |
| CaiI | CAGNNNCTG | 1 cut(s) | 35 |
| CciI | TCATGA | 1 cut(s) | 282 |
| Cfr10I | RCCGGY | 1 cut(s) | 467 |
| Cfr13I | GGNCC | 2 cut(s) | 443, 535 |
| CseI | GACGC | 2 cut(s) | 21, 108 |
| Csp6I | GTAC | 1 cut(s) | 672 |
| CspAI | ACCGGT | 1 cut(s) | 467 |
| CviAII | CATG | 3 cut(s) | 262, 283, 520 |
| CviQI | GTAC | 1 cut(s) | 672 |
| DdeI | CTNAG | 6 cut(s) | 36, 94, 183, 237, 267, 539 |
| DpnI | GATC | 2 cut(s) | 453, 546 |
| DpnII | GATC | 2 cut(s) | 451, 544 |
| EciI | GGCGGA | 1 cut(s) | 446 |
| Eco130I | CCWWGG | 2 cut(s) | 590, 681 |
| Eco31I | GGTCTC | 1 cut(s) | 717 |
| Eco32I | GATATC | 1 cut(s) | 397 |
| Eco47I | GGWCC | 2 cut(s) | 443, 535 |
| Eco81I | CCTNAGG | 1 cut(s) | 539 |
| EcoRI | GAATTC | 1 cut(s) | 753 |
| EcoRV | GATATC | 1 cut(s) | 397 |
| EcoT14I | CCWWGG | 2 cut(s) | 590, 681 |
| EcoT22I | ATGCAT | 1 cut(s) | 525 |
| ErhI | CCWWGG | 2 cut(s) | 590, 681 |
| Esp3I | CGTCTC | 1 cut(s) | 126 |
| FaeI | CATG | 3 cut(s) | 265, 286, 523 |
| FatI | CATG | 3 cut(s) | 261, 282, 519 |
| Fnu4HI | GCNGC | 1 cut(s) | 30 |
| Fsp4HI | GCNGC | 1 cut(s) | 30 |
| FspBI | CTAG | 2 cut(s) | 405, 591 |
| GluI | GCNGC | 1 cut(s) | 30 |
| HaeIII | GGCC | 1 cut(s) | 41 |
| HapII | CCGG | 1 cut(s) | 468 |
| HgaI | GACGC | 2 cut(s) | 21, 108 |
| Hin1I | GRCGYC | 1 cut(s) | 119 |
| Hin1II | CATG | 3 cut(s) | 265, 286, 523 |
| HincII | GTYRAC | 1 cut(s) | 7 |
| HindII | GTYRAC | 1 cut(s) | 7 |
| HinfI | GANTC | 2 cut(s) | 235, 353 |
| HpaII | CCGG | 1 cut(s) | 468 |
| HphI | GGTGA | 2 cut(s) | 92, 403 |
| Hpy166II | GTNNAC | 4 cut(s) | 7, 255, 446, 535 |
| Hpy188I | TCNGA | 5 cut(s) | 37, 97, 378, 563, 746 |
| Hpy188III | TCNNGA | 3 cut(s) | 245, 283, 368 |
| Hpy8I | GTNNAC | 4 cut(s) | 7, 255, 446, 535 |
| Hpy99I | CGWCG | 1 cut(s) | 567 |
| HpyAV | CCTTC | 4 cut(s) | 134, 697, 727, 771 |
| HpyCH4III | ACNGT | 2 cut(s) | 214, 259 |
| HpyCH4IV | ACGT | 2 cut(s) | 108, 565 |
| HpyCH4V | TGCA | 4 cut(s) | 479, 523, 598, 688 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 38, 604, 694 |
| HpyF3I | CTNAG | 6 cut(s) | 36, 94, 183, 237, 267, 539 |
| HpySE526I | ACGT | 2 cut(s) | 108, 565 |
| Hsp92I | GRCGYC | 1 cut(s) | 119 |
| Hsp92II | CATG | 3 cut(s) | 265, 286, 523 |
| Kzo9I | GATC | 2 cut(s) | 451, 544 |
| LmnI | GCTCC | 1 cut(s) | 363 |
| Lsp1109I | GCAGC | 1 cut(s) | 41 |
| LweI | GCATC | 1 cut(s) | 674 |
| MaeI | CTAG | 2 cut(s) | 405, 591 |
| MaeII | ACGT | 2 cut(s) | 108, 565 |
| MalI | GATC | 2 cut(s) | 453, 546 |
| MboI | GATC | 2 cut(s) | 451, 544 |
| MboII | GAAGA | 6 cut(s) | 7, 37, 149, 480, 595, 712 |
| MflI | RGATCY | 1 cut(s) | 544 |
| MhlI | GDGCHC | 3 cut(s) | 168, 300, 519 |
| MluCI | AATT | 1 cut(s) | 753 |
| MlyI | GAGTC | 2 cut(s) | 229, 347 |
| MmeI | TCCRAC | 1 cut(s) | 586 |
| MnlI | CCTC | 9 cut(s) | 31, 73, 385, 394, 523, 548, 569, 618, 768 |
| Mph1103I | ATGCAT | 1 cut(s) | 525 |
| MseI | TTAA | 3 cut(s) | 86, 224, 336 |
| MslI | CAYNNNNRTG | 1 cut(s) | 260 |
| MspI | CCGG | 1 cut(s) | 468 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 38, 604, 694 |
| NdeII | GATC | 2 cut(s) | 451, 544 |
| NheI | GCTAGC | 1 cut(s) | 404 |
| NlaIII | CATG | 3 cut(s) | 265, 286, 523 |
| NlaIV | GGNNCC | 1 cut(s) | 243 |
| NsiI | ATGCAT | 1 cut(s) | 525 |
| PagI | TCATGA | 1 cut(s) | 282 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PinAI | ACCGGT | 1 cut(s) | 467 |
| PkrI | GCNGC | 1 cut(s) | 31 |
| PleI | GAGTC | 2 cut(s) | 229, 347 |
| PpsI | GAGTC | 2 cut(s) | 229, 347 |
| PspN4I | GGNNCC | 1 cut(s) | 243 |
| PspPI | GGNCC | 2 cut(s) | 443, 535 |
| PstNI | CAGNNNCTG | 1 cut(s) | 35 |
| PsuI | RGATCY | 1 cut(s) | 544 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| RsaI | GTAC | 1 cut(s) | 673 |
| RsaNI | GTAC | 1 cut(s) | 672 |
| RseI | CAYNNNNRTG | 1 cut(s) | 260 |
| SaqAI | TTAA | 3 cut(s) | 86, 224, 336 |
| SatI | GCNGC | 1 cut(s) | 30 |
| Sau3AI | GATC | 2 cut(s) | 451, 544 |
| Sau96I | GGNCC | 2 cut(s) | 443, 535 |
| SchI | GAGTC | 2 cut(s) | 229, 347 |
| SduI | GDGCHC | 3 cut(s) | 168, 300, 519 |
| SfaNI | GCATC | 1 cut(s) | 674 |
| SfcI | CTRYAG | 2 cut(s) | 75, 195 |
| SinI | GGWCC | 2 cut(s) | 443, 535 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 260 |
| Sse9I | AATT | 1 cut(s) | 753 |
| SsiI | CCGC | 2 cut(s) | 431, 644 |
| SspI | AATATT | 1 cut(s) | 388 |
| SspMI | CTAG | 2 cut(s) | 405, 591 |
| StyI | CCWWGG | 2 cut(s) | 590, 681 |
| TaaI | ACNGT | 2 cut(s) | 214, 259 |
| TaiI | ACGT | 2 cut(s) | 111, 568 |
| TaqI | TCGA | 3 cut(s) | 48, 367, 399 |
| TasI | AATT | 1 cut(s) | 753 |
| Tru1I | TTAA | 3 cut(s) | 86, 224, 336 |
| Tru9I | TTAA | 3 cut(s) | 86, 224, 336 |
| TscAI | CASTG | 2 cut(s) | 432, 498 |
| TseI | GCWGC | 1 cut(s) | 29 |
| TspDTI | ATGAA | 3 cut(s) | 206, 271, 618 |
| TspRI | CASTG | 2 cut(s) | 432, 498 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VpaK11BI | GGWCC | 2 cut(s) | 443, 535 |
| XapI | RAATTY | 1 cut(s) | 753 |
| XmaJI | CCTAGG | 1 cut(s) | 590 |
| XspI | CTAG | 2 cut(s) | 405, 591 |
| Zsp2I | ATGCAT | 1 cut(s) | 525 |