Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 610 |
| AccB1I | GGYRCC | 1 cut(s) | 610 |
| AciI | CCGC | 3 cut(s) | 87, 131, 374 |
| AclWI | GGATC | 2 cut(s) | 498, 511 |
| AcsI | RAATTY | 1 cut(s) | 400 |
| AfaI | GTAC | 3 cut(s) | 66, 586, 612 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 607 |
| AflIII | ACRYGT | 1 cut(s) | 426 |
| AgsI | TTSAA | 2 cut(s) | 448, 640 |
| AhlI | ACTAGT | 2 cut(s) | 173, 587 |
| AjiI | CACGTC | 1 cut(s) | 228 |
| AjnI | CCWGG | 2 cut(s) | 247, 453 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 463, 495 |
| AluBI | AGCT | 4 cut(s) | 160, 563, 605, 688 |
| AluI | AGCT | 4 cut(s) | 160, 563, 605, 688 |
| Alw26I | GTCTC | 2 cut(s) | 352, 392 |
| AlwI | GGATC | 2 cut(s) | 498, 511 |
| AoxI | GGCC | 3 cut(s) | 120, 524, 650 |
| ApeKI | GCWGC | 2 cut(s) | 23, 563 |
| ApoI | RAATTY | 1 cut(s) | 400 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 260, 292 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 404 |
| Asp718I | GGTACC | 1 cut(s) | 610 |
| AspLEI | GCGC | 2 cut(s) | 28, 645 |
| AspS9I | GGNCC | 1 cut(s) | 376 |
| AsuHPI | GGTGA | 4 cut(s) | 342, 521, 634, 710 |
| AsuII | TTCGAA | 1 cut(s) | 279 |
| AvaII | GGWCC | 1 cut(s) | 376 |
| BamHI | GGATCC | 1 cut(s) | 503 |
| BanI | GGYRCC | 1 cut(s) | 610 |
| BbvI | GCAGC | 2 cut(s) | 10, 550 |
| BccI | CCATC | 2 cut(s) | 8, 116 |
| BciT130I | CCWGG | 2 cut(s) | 249, 455 |
| BcoDI | GTCTC | 2 cut(s) | 352, 392 |
| BcuI | ACTAGT | 2 cut(s) | 173, 587 |
| BfaI | CTAG | 4 cut(s) | 174, 519, 588, 602 |
| BfmI | CTRYAG | 1 cut(s) | 483 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 128 |
| BisI | GCNGC | 2 cut(s) | 24, 564 |
| BlsI | GCNGC | 2 cut(s) | 25, 565 |
| Bme1390I | CCNGG | 2 cut(s) | 249, 455 |
| Bme18I | GGWCC | 1 cut(s) | 376 |
| BmgBI | CACGTC | 1 cut(s) | 228 |
| BmgT120I | GGNCC | 1 cut(s) | 376 |
| BmiI | GGNNCC | 2 cut(s) | 505, 612 |
| BmrFI | CCNGG | 2 cut(s) | 249, 455 |
| BmrI | ACTGGG | 1 cut(s) | 727 |
| BmsI | GCATC | 4 cut(s) | 78, 372, 586, 713 |
| BmuI | ACTGGG | 1 cut(s) | 727 |
| BpmI | CTGGAG | 1 cut(s) | 270 |
| Bpu14I | TTCGAA | 1 cut(s) | 279 |
| BpuEI | CTTGAG | 1 cut(s) | 668 |
| BsaI | GGTCTC | 1 cut(s) | 352 |
| BsaJI | CCNNGG | 1 cut(s) | 454 |
| BsaWI | WCCGGW | 1 cut(s) | 506 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 607 |
| Bse1I | ACTGG | 1 cut(s) | 722 |
| Bse3DI | GCAATG | 1 cut(s) | 547 |
| BseBI | CCWGG | 2 cut(s) | 249, 455 |
| BseDI | CCNNGG | 1 cut(s) | 454 |
| BseGI | GGATG | 2 cut(s) | 78, 601 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 607 |
| BseMI | GCAATG | 1 cut(s) | 547 |
| BseMII | CTCAG | 4 cut(s) | 252, 357, 411, 570 |
| BseNI | ACTGG | 1 cut(s) | 722 |
| BseRI | GAGGAG | 1 cut(s) | 658 |
| BseXI | GCAGC | 2 cut(s) | 10, 550 |
| BshFI | GGCC | 3 cut(s) | 122, 526, 652 |
| BshNI | GGYRCC | 1 cut(s) | 610 |
| BsiSI | CCGG | 1 cut(s) | 507 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 607 |
| BsmAI | GTCTC | 2 cut(s) | 352, 392 |
| BsmBI | CGTCTC | 1 cut(s) | 392 |
| BsmI | GAATGC | 1 cut(s) | 443 |
| BsnI | GGCC | 3 cut(s) | 122, 526, 652 |
| Bso31I | GGTCTC | 1 cut(s) | 352 |
| Bsp119I | TTCGAA | 1 cut(s) | 279 |
| Bsp143I | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| BspACI | CCGC | 3 cut(s) | 87, 131, 374 |
| BspANI | GGCC | 3 cut(s) | 122, 526, 652 |
| BspCNI | CTCAG | 4 cut(s) | 253, 358, 412, 571 |
| BspHI | TCATGA | 1 cut(s) | 111 |
| BspLI | GGNNCC | 2 cut(s) | 505, 612 |
| BspPI | GGATC | 2 cut(s) | 498, 511 |
| BspT104I | TTCGAA | 1 cut(s) | 279 |
| BspT107I | GGYRCC | 1 cut(s) | 610 |
| BspTNI | GGTCTC | 1 cut(s) | 352 |
| BsrDI | GCAATG | 1 cut(s) | 547 |
| BsrI | ACTGG | 1 cut(s) | 722 |
| BssECI | CCNNGG | 1 cut(s) | 454 |
| BssMI | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| Bst2UI | CCWGG | 2 cut(s) | 249, 455 |
| Bst4CI | ACNGT | 1 cut(s) | 354 |
| Bst6I | CTCTTC | 1 cut(s) | 90 |
| BstBI | TTCGAA | 1 cut(s) | 279 |
| BstC8I | GCNNGC | 3 cut(s) | 359, 490, 630 |
| BstDEI | CTNAG | 4 cut(s) | 261, 366, 420, 579 |
| BstF5I | GGATG | 2 cut(s) | 78, 601 |
| BstHHI | GCGC | 2 cut(s) | 28, 645 |
| BstKTI | GATC | 5 cut(s) | 154, 183, 291, 506, 578 |
| BstMAI | GTCTC | 2 cut(s) | 352, 392 |
| BstMBI | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 128, 649 |
| BstNI | CCWGG | 2 cut(s) | 249, 455 |
| BstNSI | RCATGY | 1 cut(s) | 430 |
| BstSCI | CCNGG | 2 cut(s) | 247, 453 |
| BstSFI | CTRYAG | 1 cut(s) | 483 |
| BstV1I | GCAGC | 2 cut(s) | 10, 550 |
| BstX2I | RGATCY | 1 cut(s) | 503 |
| BstYI | RGATCY | 1 cut(s) | 503 |
| BsuRI | GGCC | 3 cut(s) | 122, 526, 652 |
| BtrI | CACGTC | 1 cut(s) | 228 |
| BtsCI | GGATG | 2 cut(s) | 78, 601 |
| Cac8I | GCNNGC | 3 cut(s) | 359, 490, 630 |
| CciI | TCATGA | 1 cut(s) | 111 |
| CfoI | GCGC | 2 cut(s) | 28, 645 |
| Cfr13I | GGNCC | 1 cut(s) | 376 |
| CseI | GACGC | 2 cut(s) | 374, 634 |
| Csp6I | GTAC | 3 cut(s) | 65, 585, 611 |
| CviAII | CATG | 5 cut(s) | 112, 200, 323, 409, 427 |
| CviJI | RGCY | 9 cut(s) | 122, 160, 260, 414, 526, 563, 605, 652, 688 |
| CviKI_1 | RGCY | 9 cut(s) | 122, 160, 260, 414, 526, 563, 605, 652, 688 |
| CviQI | GTAC | 3 cut(s) | 65, 585, 611 |
| DdeI | CTNAG | 4 cut(s) | 261, 366, 420, 579 |
| DpnI | GATC | 5 cut(s) | 153, 182, 290, 505, 577 |
| DpnII | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| Eam1104I | CTCTTC | 1 cut(s) | 90 |
| EarI | CTCTTC | 1 cut(s) | 90 |
| Eco31I | GGTCTC | 1 cut(s) | 352 |
| Eco32I | GATATC | 1 cut(s) | 205 |
| Eco47I | GGWCC | 1 cut(s) | 376 |
| EcoRI | GAATTC | 1 cut(s) | 400 |
| EcoRII | CCWGG | 2 cut(s) | 247, 453 |
| EcoRV | GATATC | 1 cut(s) | 205 |
| Esp3I | CGTCTC | 1 cut(s) | 392 |
| FaeI | CATG | 5 cut(s) | 115, 203, 326, 412, 430 |
| FatI | CATG | 5 cut(s) | 111, 199, 322, 408, 426 |
| FauI | CCCGC | 2 cut(s) | 94, 124 |
| Fnu4HI | GCNGC | 2 cut(s) | 24, 564 |
| FokI | GGATG | 2 cut(s) | 65, 608 |
| Fsp4HI | GCNGC | 2 cut(s) | 24, 564 |
| FspBI | CTAG | 4 cut(s) | 174, 519, 588, 602 |
| GlaI | GCGC | 2 cut(s) | 27, 644 |
| GluI | GCNGC | 2 cut(s) | 24, 564 |
| GsuI | CTGGAG | 1 cut(s) | 270 |
| HaeIII | GGCC | 3 cut(s) | 122, 526, 652 |
| HapII | CCGG | 1 cut(s) | 507 |
| HgaI | GACGC | 2 cut(s) | 374, 634 |
| HhaI | GCGC | 2 cut(s) | 28, 645 |
| Hin1II | CATG | 5 cut(s) | 115, 203, 326, 412, 430 |
| Hin6I | GCGC | 2 cut(s) | 26, 643 |
| HinP1I | GCGC | 2 cut(s) | 26, 643 |
| HindIII | AAGCTT | 1 cut(s) | 686 |
| HinfI | GANTC | 2 cut(s) | 232, 722 |
| HpaII | CCGG | 1 cut(s) | 507 |
| HphI | GGTGA | 4 cut(s) | 342, 521, 634, 710 |
| Hpy166II | GTNNAC | 1 cut(s) | 231 |
| Hpy188I | TCNGA | 4 cut(s) | 237, 367, 580, 709 |
| Hpy188III | TCNNGA | 3 cut(s) | 112, 223, 519 |
| Hpy8I | GTNNAC | 1 cut(s) | 231 |
| HpyAV | CCTTC | 3 cut(s) | 489, 516, 673 |
| HpyCH4III | ACNGT | 1 cut(s) | 354 |
| HpyCH4IV | ACGT | 1 cut(s) | 227 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 357, 443, 463, 540, 566 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 128, 649 |
| HpyF3I | CTNAG | 4 cut(s) | 261, 366, 420, 579 |
| HpySE526I | ACGT | 1 cut(s) | 227 |
| Hsp92II | CATG | 5 cut(s) | 115, 203, 326, 412, 430 |
| HspAI | GCGC | 2 cut(s) | 26, 643 |
| KpnI | GGTACC | 1 cut(s) | 614 |
| Kzo9I | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| Lsp1109I | GCAGC | 2 cut(s) | 10, 550 |
| LweI | GCATC | 4 cut(s) | 78, 372, 586, 713 |
| MaeI | CTAG | 4 cut(s) | 174, 519, 588, 602 |
| MaeII | ACGT | 1 cut(s) | 227 |
| MaeIII | GTNAC | 3 cut(s) | 195, 308, 622 |
| MalI | GATC | 5 cut(s) | 153, 182, 290, 505, 577 |
| MboI | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| MboII | GAAGA | 3 cut(s) | 107, 265, 268 |
| MflI | RGATCY | 1 cut(s) | 503 |
| MluCI | AATT | 2 cut(s) | 315, 400 |
| MlyI | GAGTC | 1 cut(s) | 226 |
| MnlI | CCTC | 8 cut(s) | 39, 62, 133, 361, 402, 574, 613, 679 |
| MroXI | GAANNNNTTC | 1 cut(s) | 404 |
| MseI | TTAA | 1 cut(s) | 435 |
| MslI | CAYNNNNRTG | 1 cut(s) | 116 |
| MspI | CCGG | 1 cut(s) | 507 |
| MspR9I | CCNGG | 2 cut(s) | 249, 455 |
| Mva1269I | GAATGC | 1 cut(s) | 443 |
| MvaI | CCWGG | 2 cut(s) | 249, 455 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 128, 649 |
| NdeII | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| NlaIII | CATG | 5 cut(s) | 115, 203, 326, 412, 430 |
| NlaIV | GGNNCC | 2 cut(s) | 505, 612 |
| NmuCI | GTSAC | 2 cut(s) | 195, 622 |
| NspI | RCATGY | 1 cut(s) | 430 |
| NspV | TTCGAA | 1 cut(s) | 279 |
| PagI | TCATGA | 1 cut(s) | 111 |
| PciI | ACATGT | 1 cut(s) | 426 |
| PctI | GAATGC | 1 cut(s) | 443 |
| PdmI | GAANNNNTTC | 1 cut(s) | 404 |
| PfeI | GAWTC | 1 cut(s) | 722 |
| PfoI | TCCNGGA | 1 cut(s) | 247 |
| PkrI | GCNGC | 2 cut(s) | 25, 565 |
| PleI | GAGTC | 1 cut(s) | 226 |
| PpsI | GAGTC | 1 cut(s) | 226 |
| PscI | ACATGT | 1 cut(s) | 426 |
| Psp6I | CCWGG | 2 cut(s) | 247, 453 |
| PspGI | CCWGG | 2 cut(s) | 247, 453 |
| PspN4I | GGNNCC | 2 cut(s) | 505, 612 |
| PspPI | GGNCC | 1 cut(s) | 376 |
| PsuI | RGATCY | 1 cut(s) | 503 |
| RsaI | GTAC | 3 cut(s) | 66, 586, 612 |
| RsaNI | GTAC | 3 cut(s) | 65, 585, 611 |
| RseI | CAYNNNNRTG | 1 cut(s) | 116 |
| SaqAI | TTAA | 1 cut(s) | 435 |
| SatI | GCNGC | 2 cut(s) | 24, 564 |
| Sau3AI | GATC | 5 cut(s) | 151, 180, 288, 503, 575 |
| Sau96I | GGNCC | 1 cut(s) | 376 |
| SchI | GAGTC | 1 cut(s) | 226 |
| ScrFI | CCNGG | 2 cut(s) | 249, 455 |
| SfaNI | GCATC | 4 cut(s) | 78, 372, 586, 713 |
| SfcI | CTRYAG | 1 cut(s) | 483 |
| SfuI | TTCGAA | 1 cut(s) | 279 |
| SinI | GGWCC | 1 cut(s) | 376 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 116 |
| SmlI | CTYRAG | 1 cut(s) | 683 |
| SmoI | CTYRAG | 1 cut(s) | 683 |
| SpeI | ACTAGT | 2 cut(s) | 173, 587 |
| Sse9I | AATT | 2 cut(s) | 315, 400 |
| SsiI | CCGC | 3 cut(s) | 87, 131, 374 |
| SspMI | CTAG | 4 cut(s) | 174, 519, 588, 602 |
| StyD4I | CCNGG | 2 cut(s) | 247, 453 |
| TaaI | ACNGT | 1 cut(s) | 354 |
| TaiI | ACGT | 1 cut(s) | 230 |
| TaqI | TCGA | 2 cut(s) | 279, 574 |
| TasI | AATT | 2 cut(s) | 315, 400 |
| TfiI | GAWTC | 1 cut(s) | 722 |
| Tru1I | TTAA | 1 cut(s) | 435 |
| Tru9I | TTAA | 1 cut(s) | 435 |
| TseFI | GTSAC | 2 cut(s) | 195, 622 |
| TseI | GCWGC | 2 cut(s) | 23, 563 |
| Tsp45I | GTSAC | 2 cut(s) | 195, 622 |
| TspDTI | ATGAA | 3 cut(s) | 128, 393, 397 |
| VpaK11BI | GGWCC | 1 cut(s) | 376 |
| XapI | RAATTY | 1 cut(s) | 400 |
| XbaI | TCTAGA | 1 cut(s) | 518 |
| XceI | RCATGY | 1 cut(s) | 430 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 115 |
| XmnI | GAANNNNTTC | 1 cut(s) | 404 |
| XspI | CTAG | 4 cut(s) | 174, 519, 588, 602 |