Rh5DG399000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000032
Physical Location & Seq
Reverse (-)
62899805 .. 62900256
452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG399000.1

Sequence Viewer

Length: 279 bp
ATGGGGGCTTTGTGCATGGGAGCTTCAGCTTTAAAGGAGAGAAGTTCACAACCTTTGGTTCCGATTGATACAAATATTGAGCCTGAGGAGGTTGCAGAGCTTATACCATCTGAAGGGGGCTCTATAGATGTGAATGCACATCAAGTACTAGATGAGACTGTTTGTGTAAACCAGGAGAAAATTACTGATAAACACGGGGAAATTGAGAAACAGGTTGATGACACTGCTGATGTGGATGGCATGCCTGTGGCGCCGGAACTTCAAGCTAAAGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

9.78

Weight (kDa)

4.12

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000032)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20531 FvH4_1g22101 FvH4_1g26161 FvH4_2g06802 FvH4_2g07851 FvH4_2g07920 FvH4_2g11481 FvH4_2g14491 FvH4_2g14510 FvH4_3g02671 FvH4_3g10870 FvH4_3g16811 FvH4_3g18871 FvH4_3g20011 FvH4_3g29181 FvH4_3g30101 FvH4_3g33941 FvH4_4g08031 FvH4_4g10192 FvH4_4g20791 FvH4_5g02440 FvH4_5g03261 FvH4_5g15791 FvH4_5g22472 FvH4_5g23680 FvH4_5g26530 FvH4_5g26540 FvH4_5g30241 FvH4_5g32311 FvH4_5g38943 FvH4_6g05152 FvH4_6g10481 FvH4_6g14353 FvH4_6g17850 FvH4_6g19460 FvH4_6g23861 FvH4_6g30881 FvH4_6g31722 FvH4_6g40031 FvH4_6g46031 FvH4_6g49021 FvH4_7g12530 FvH4_7g15960
pyrus_communis pycom09g18450
rosa_chinensis RchiOBHm_Chr1g0341771 RchiOBHm_Chr1g0354791 RchiOBHm_Chr2g0095751 RchiOBHm_Chr5g0030691 RchiOBHm_Chr7g0181741 RchiOBHm_Chr7g0222721 RchiOBHm_Chr7g0243191
rosa_laevigata RLG00000001405 RLG00000001878 RLG00000002053 RLG00000002361 RLG00000003846 RLG00000012639 RLG00000013326 RLG00000014331 RLG00000017340 RLG00000017548 RLG00000018177 RLG00000018372 RLG00000019006 RLG00000019406 RLG00000019522 RLG00000019922 RLG00000020489 RLG00000022399 RLG00000023433 RLG00000027372 RLG00000028576 RLG00000028590 RLG00000028947 RLG00000032552 RLG00000033183 RLG00000033245 RLG00000033730 RLG00000034443 RLG00000034772 RLG00000036008
rosa_multiflora Rmu_co8029972.1_g000001 Rmu_co8044978.1_g000001 Rmu_co8338363.1_g000001 Rmu_co8426431.1_g000002 Rmu_co8457575.1_g000001 Rmu_sc0000010.1_g000025 Rmu_sc0000197.1_g000018 Rmu_sc0000220.1_g000009 Rmu_sc0000221.1_g000012 Rmu_sc0000258.1_g000002 Rmu_sc0000284.1_g000015 Rmu_sc0000303.1_g000021 Rmu_sc0000309.1_g000015 Rmu_sc0000317.1_g000017 Rmu_sc0000355.1_g000003 Rmu_sc0000376.1_g000035 Rmu_sc0000420.1_g000009 Rmu_sc0000493.1_g000019 Rmu_sc0000498.1_g000044 Rmu_sc0000540.1_g000015 Rmu_sc0000554.1_g000031 Rmu_sc0000577.1_g000004 Rmu_sc0000647.1_g000007 Rmu_sc0000703.1_g000002 Rmu_sc0000776.1_g000025 Rmu_sc0000924.1_g000044 Rmu_sc0000940.1_g000034 Rmu_sc0000940.1_g000036 Rmu_sc0000990.1_g000014 Rmu_sc0001004.1_g000018 Rmu_sc0001027.1_g000014 Rmu_sc0001059.1_g000004 Rmu_sc0001089.1_g000021 Rmu_sc0001118.1_g000013 Rmu_sc0001141.1_g000024 Rmu_sc0001154.1_g000002 Rmu_sc0001179.1_g000023 Rmu_sc0001230.1_g000014 Rmu_sc0001298.1_g000042 Rmu_sc0001306.1_g000030 Rmu_sc0001306.1_g000036 Rmu_sc0001341.1_g000007 Rmu_sc0001386.1_g000013 Rmu_sc0001461.1_g000019 Rmu_sc0001461.1_g000047 Rmu_sc0001476.1_g000013 Rmu_sc0001516.1_g000032 Rmu_sc0001780.1_g000007 Rmu_sc0001793.1_g000005 Rmu_sc0001911.1_g000013 Rmu_sc0001962.1_g000011 Rmu_sc0001986.1_g000004 Rmu_sc0002061.1_g000021 Rmu_sc0002119.1_g000024 Rmu_sc0002194.1_g000021 Rmu_sc0002284.1_g000019 Rmu_sc0002316.1_g000014 Rmu_sc0002322.1_g000006 Rmu_sc0002351.1_g000107 Rmu_sc0002387.1_g000008 Rmu_sc0002389.1_g000029 Rmu_sc0002507.1_g000001 Rmu_sc0002589.1_g000038 Rmu_sc0002638.1_g000041 Rmu_sc0002656.1_g000007 Rmu_sc0002882.1_g000030 Rmu_sc0002927.1_g000034 Rmu_sc0003024.1_g000020 Rmu_sc0003032.1_g000027 Rmu_sc0003069.1_g000025 Rmu_sc0003268.1_g000002 Rmu_sc0003296.1_g000007 Rmu_sc0003363.1_g000073 Rmu_sc0003527.1_g000017 Rmu_sc0003638.1_g000007 Rmu_sc0003669.1_g000007 Rmu_sc0003882.1_g000021 Rmu_sc0003941.1_g000004 Rmu_sc0003979.1_g000007 Rmu_sc0003983.1_g000007 Rmu_sc0003989.1_g000017 Rmu_sc0004016.1_g000003 Rmu_sc0004020.1_g000025 Rmu_sc0004094.1_g000016 Rmu_sc0004135.1_g000002 Rmu_sc0004154.1_g000014 Rmu_sc0004316.1_g000002 Rmu_sc0004316.1_g000015 Rmu_sc0004321.1_g000013 Rmu_sc0004467.1_g000005 Rmu_sc0004517.1_g000007 Rmu_sc0004642.1_g000012 Rmu_sc0004729.1_g000024 Rmu_sc0004757.1_g000018 Rmu_sc0004788.1_g000003 Rmu_sc0004835.1_g000026 Rmu_sc0004889.1_g000019 Rmu_sc0004991.1_g000009 Rmu_sc0005045.1_g000004 Rmu_sc0005079.1_g000004 Rmu_sc0005083.1_g000008 Rmu_sc0005170.1_g000002 Rmu_sc0005190.1_g000009 Rmu_sc0005217.1_g000010 Rmu_sc0005234.1_g000011 Rmu_sc0005608.1_g000016 Rmu_sc0005802.1_g000029 Rmu_sc0005810.1_g000006 Rmu_sc0005904.1_g000006 Rmu_sc0005967.1_g000012 Rmu_sc0006028.1_g000027 Rmu_sc0006059.1_g000040 Rmu_sc0006423.1_g000002 Rmu_sc0006467.1_g000001 Rmu_sc0006470.1_g000004 Rmu_sc0006598.1_g000003 Rmu_sc0006599.1_g000004 Rmu_sc0006632.1_g000021 Rmu_sc0006707.1_g000008 Rmu_sc0006791.1_g000008 Rmu_sc0006835.1_g000016 Rmu_sc0007100.1_g000002 Rmu_sc0007115.1_g000009 Rmu_sc0007362.1_g000012 Rmu_sc0007461.1_g000004 Rmu_sc0007474.1_g000001 Rmu_sc0007681.1_g000011 Rmu_sc0007697.1_g000003 Rmu_sc0007704.1_g000001 Rmu_sc0007722.1_g000016 Rmu_sc0007843.1_g000001 Rmu_sc0007856.1_g000009 Rmu_sc0008328.1_g000007 Rmu_sc0008780.1_g000005 Rmu_sc0009185.1_g000011 Rmu_sc0009599.1_g000021 Rmu_sc0009881.1_g000002 Rmu_sc0009891.1_g000002 Rmu_sc0010357.1_g000003 Rmu_sc0010781.1_g000005 Rmu_sc0011133.1_g000005 Rmu_sc0012334.1_g000001 Rmu_sc0012569.1_g000015 Rmu_sc0012984.1_g000001 Rmu_sc0013508.1_g000001 Rmu_sc0013550.1_g000005 Rmu_sc0013730.1_g000001 Rmu_sc0013945.1_g000001 Rmu_sc0013945.1_g000003 Rmu_sc0014005.1_g000005 Rmu_sc0014111.1_g000009 Rmu_sc0014111.1_g000010 Rmu_sc0014203.1_g000025 Rmu_sc0014839.1_g000002 Rmu_sc0015410.1_g000001 Rmu_sc0015513.1_g000003 Rmu_sc0015791.1_g000008 Rmu_sc0016657.1_g000004 Rmu_sc0017187.1_g000002 Rmu_sc0017400.1_g000001 Rmu_sc0017895.1_g000006 Rmu_sc0018221.1_g000008 Rmu_sc0018825.1_g000002 Rmu_sc0019047.1_g000001 Rmu_sc0019077.1_g000001 Rmu_sc0019171.1_g000001 Rmu_sc0019299.1_g000002 Rmu_sc0020342.1_g000008 Rmu_sc0020688.1_g000002 Rmu_sc0020688.1_g000003 Rmu_sc0021871.1_g000012 Rmu_sc0022925.1_g000001 Rmu_sc0023965.1_g000005 Rmu_sc0024968.1_g000001 Rmu_sc0024969.1_g000001 Rmu_sc0025035.1_g000002 Rmu_sc0025167.1_g000005 Rmu_sc0025567.1_g000001 Rmu_sc0026096.1_g000001 Rmu_sc0026586.1_g000001 Rmu_sc0028358.1_g000002 Rmu_sc0032171.1_g000001 Rmu_sc0034632.1_g000001 Rmu_sc0034633.1_g000001 Rmu_sc0035481.1_g000001 Rmu_sc0036303.1_g000001 Rmu_sc0039774.1_g000001 Rmu_sc0040023.1_g000002 Rmu_sc0040790.1_g000001 Rmu_sc0041574.1_g000001 Rmu_sc0042768.1_g000003 Rmu_ssc0000038.1_g000016 Rmu_ssc0000062.1_g000035 Rmu_ssc0000083.1_g000009 Rmu_ssc0000093.1_g000006 Rmu_ssc0000112.1_g000023 Rmu_ssc0000155.1_g000007 Rmu_ssc0000171.1_g000017 Rmu_ssc0000196.1_g000017 Rmu_ssc0000257.1_g000027 Rmu_ssc0000323.1_g000023 Rmu_ssc0000440.1_g000011 Rmu_ssc0000454.1_g000021 Rmu_ssc0000473.1_g000011
rosa_roxburghii Rroxscaffold_1G00014010 Rroxscaffold_1G00039470 Rroxscaffold_1G00045350 Rroxscaffold_2G00082440 Rroxscaffold_2G00096630 Rroxscaffold_2G00100560 Rroxscaffold_2G00102600 Rroxscaffold_2G00117720 Rroxscaffold_2G00120390 Rroxscaffold_2G00126010 Rroxscaffold_3G00220680 Rroxscaffold_3G00241760 Rroxscaffold_4G00309670 Rroxscaffold_4G00312380 Rroxscaffold_5G00339950 Rroxscaffold_5G00361980 Rroxscaffold_5G00370500 Rroxscaffold_5G00376480 Rroxscaffold_5G00384070 Rroxscaffold_6G00393690 Rroxscaffold_6G00396520 Rroxscaffold_6G00396850 Rroxscaffold_6G00404790 Rroxscaffold_7G00177690 Rroxscaffold_7G00178170 Rroxscaffold_7G00194330 Rroxscaffold_7G00197090 Rroxscaffold_9G00437910
rosa_rugosa Rorug01G0010100 Rorug01G0035300 Rorug01G0038600 Rorug01G0038700 Rorug01G0050300 Rorug01G0143800.1 Rorug01G0211700 Rorug01G0266600 Rorug01G0268800 Rorug01G0301200 Rorug02G0139800 Rorug02G0247100 Rorug02G0247100 Rorug02G0276800 Rorug02G0283300 Rorug02G0289800 Rorug02G0326700 Rorug02G0368100 Rorug02G0427000 Rorug02G0439100 Rorug02G0611200 Rorug02G0626600 Rorug03G0049100 Rorug03G0108000 Rorug03G0118600 Rorug03G0174300.1 Rorug03G0182300 Rorug03G0189800 Rorug03G0207000 Rorug03G0220700 Rorug03G0229400 Rorug03G0253700 Rorug03G0276500 Rorug03G0283300.1 Rorug03G0283500 Rorug03G0283600 Rorug03G0299600 Rorug04G0052200 Rorug04G0052300 Rorug04G0115800 Rorug04G0159700 Rorug04G0170900 Rorug04G0178100 Rorug04G0250800 Rorug04G0317200 Rorug04G0409500 Rorug05G0022500 Rorug05G0134900 Rorug05G0144800 Rorug05G0158200 Rorug05G0168300 Rorug05G0168400 Rorug05G0168600 Rorug05G0229800 Rorug05G0259100 Rorug05G0285700 Rorug05G0294600 Rorug05G0306400 Rorug05G0335300 Rorug05G0390100 Rorug05G0392900 Rorug05G0407100 Rorug05G0416600 Rorug05G0536900 Rorug05G0550300 Rorug06G0040900 Rorug06G0042800 Rorug06G0093000 Rorug06G0110700 Rorug06G0115600 Rorug06G0205700 Rorug07G0016900.1 Rorug07G0017000 Rorug07G0095200 Rorug07G0106400 Rorug07G0116400 Rorug07G0127300 Rorug07G0151100 Rorug07G0151200 Rorug07G0178200 Rorug07G0224300 Rorug07G0224400 Rorug07G0226400 Rorug07G0258200 Rorug07G0328000 RorugPtG0002700.1
rosa_samantha Rh1BG231600 Rh1BG235500 Rh1DG259300 Rh2AG044200 Rh2AG095600 Rh2AG164800 Rh2AG361400 Rh2AG370600 Rh2AG487800 Rh2AG620400 Rh2BG171900 Rh2BG244600 Rh2BG500300 Rh2CG189500 Rh2CG317600 Rh2CG374400 Rh2CG474300 Rh2DG170300 Rh2DG374800 Rh2DG511500 Rh3AG273400 Rh3AG331300 Rh3CG203900 Rh3CG222200 Rh3CG253800 Rh3CG269900 Rh3DG073800 Rh3DG208100 Rh3DG224000 Rh3DG265900 Rh4AG217200 Rh4BG076000 Rh4BG113200 Rh4BG240700 Rh4BG414300 Rh4CG266600 Rh4CG307600 Rh5BG088500 Rh5BG469000 Rh5CG284400 Rh5CG491200 Rh5CG557400 Rh5DG399000 Rh6AG457400 Rh6BG339800 Rh6BG443700 Rh6CG115400 Rh6CG471500 Rh6DG390100 Rh6DG457900 Rh7AG372100 Rh7AG429800 Rh7AG484600 Rh7BG176400 Rh7BG233900 Rh7BG244400 Rh7BG279800 Rh7BG285100 Rh7CG172200 Rh7CG390500 Rh7CG502600 Rh7CG518200 Rh7DG252500 Rh7DG294200 Rh7DG376400 Rh7DG390100
rosa_wichuraiana Rw0G001650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AcuI CTGAAG 2 cut(s) 9, 132
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 113
AgsI TTSAA 1 cut(s) 263
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 4 cut(s) 23, 29, 100, 266
AluI AGCT 4 cut(s) 23, 29, 100, 266
Alw26I GTCTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 253
AxyI CCTNAGG 1 cut(s) 84
BanI GGYRCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 122
BccI CCATC 2 cut(s) 115, 230
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 2 cut(s) 149, 277
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 254
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 60, 252
BmrFI CCNGG 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse21I CCTNAGG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 173
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 60, 252
BspT107I GGYRCC 1 cut(s) 250
BssNI GRCGYC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 160
BstACI GRCGYC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstMAI GTCTC 1 cut(s) 149
BstMWI GCNNNNNNNGC 1 cut(s) 250
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 123
Bsu36I CCTNAGG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 242
CfoI GCGC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 146
CviAII CATG 2 cut(s) 16, 241
CviJI RGCY 8 cut(s) 8, 23, 29, 82, 100, 120, 266, 276
CviKI_1 RGCY 8 cut(s) 8, 23, 29, 82, 100, 120, 266, 276
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 1 cut(s) 84
DinI GGCGCC 1 cut(s) 252
DraI TTTAAA 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 122
Eco57I CTGAAG 2 cut(s) 9, 132
Eco81I CCTNAGG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 122
EgeI GGCGCC 1 cut(s) 252
EheI GGCGCC 1 cut(s) 252
FaeI CATG 2 cut(s) 19, 244
FaiI YATR 4 cut(s) 17, 104, 125, 242
FatI CATG 2 cut(s) 15, 240
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 122
FspBI CTAG 2 cut(s) 149, 277
GlaI GCGC 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 254
HapII CCGG 1 cut(s) 254
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 2 cut(s) 19, 244
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HpaII CCGG 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 47, 169
Hpy188I TCNGA 2 cut(s) 63, 112
Hpy8I GTNNAC 2 cut(s) 47, 169
HpyAV CCTTC 2 cut(s) 107, 266
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 15, 95, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 250
HpyF3I CTNAG 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 2 cut(s) 19, 244
HspAI GCGC 1 cut(s) 251
KasI GGCGCC 1 cut(s) 250
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 6 cut(s) 96, 158, 185, 197, 258, 267
MaeI CTAG 2 cut(s) 149, 277
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 2 cut(s) 180, 201
Mly113I GGCGCC 1 cut(s) 251
MnlI CCTC 2 cut(s) 79, 82
MseI TTAA 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 139
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 251
NlaIII CATG 2 cut(s) 19, 244
NlaIV GGNNCC 2 cut(s) 60, 252
NspI RCATGY 1 cut(s) 244
PaeI GCATGC 1 cut(s) 244
PctI GAATGC 1 cut(s) 139
PluTI GGCGCC 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 60, 252
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 32
ScaI AGTACT 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 1 cut(s) 122
SetI ASST 7 cut(s) 25, 31, 55, 93, 102, 216, 268
SfcI CTRYAG 1 cut(s) 123
SfoI GGCGCC 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 245
SphI GCATGC 1 cut(s) 244
Sse9I AATT 2 cut(s) 180, 201
SspDI GGCGCC 1 cut(s) 250
SspI AATATT 1 cut(s) 76
SspMI CTAG 2 cut(s) 149, 277
StyD4I CCNGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 160
TasI AATT 2 cut(s) 180, 201
TatI WGTACW 1 cut(s) 145
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 1 cut(s) 229
TspRI CASTG 1 cut(s) 229
XceI RCATGY 1 cut(s) 244
XspI CTAG 2 cut(s) 149, 277
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.