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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 375, 659 |
| AccB1I | GGYRCC | 1 cut(s) | 157 |
| AciI | CCGC | 2 cut(s) | 103, 693 |
| AclWI | GGATC | 1 cut(s) | 617 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcyI | GRCGYC | 1 cut(s) | 158 |
| AdeI | CACNNNGTG | 1 cut(s) | 646 |
| AfaI | GTAC | 1 cut(s) | 303 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 266, 490, 747 |
| AgsI | TTSAA | 4 cut(s) | 215, 288, 415, 530 |
| AjiI | CACGTC | 1 cut(s) | 131 |
| AjnI | CCWGG | 1 cut(s) | 334 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 522, 554 |
| AluBI | AGCT | 5 cut(s) | 82, 172, 409, 554, 768 |
| AluI | AGCT | 5 cut(s) | 82, 172, 409, 554, 768 |
| Alw21I | GWGCWC | 1 cut(s) | 317 |
| Alw44I | GTGCAC | 1 cut(s) | 313 |
| AlwI | GGATC | 1 cut(s) | 617 |
| AoxI | GGCC | 2 cut(s) | 3, 417 |
| ApaLI | GTGCAC | 1 cut(s) | 313 |
| ApeKI | GCWGC | 3 cut(s) | 23, 320, 558 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 149, 464 |
| AspLEI | GCGC | 5 cut(s) | 92, 160, 186, 448, 764 |
| AspS9I | GGNCC | 2 cut(s) | 193, 294 |
| AsuHPI | GGTGA | 2 cut(s) | 437, 682 |
| AsuNHI | GCTAGC | 1 cut(s) | 764 |
| AvaII | GGWCC | 2 cut(s) | 193, 294 |
| BaeGI | GKGCMC | 1 cut(s) | 317 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanI | GGYRCC | 1 cut(s) | 157 |
| BanII | GRGCYC | 1 cut(s) | 110 |
| BbsI | GAAGAC | 1 cut(s) | 239 |
| Bbv12I | GWGCWC | 1 cut(s) | 317 |
| BbvI | GCAGC | 3 cut(s) | 10, 307, 570 |
| BccI | CCATC | 2 cut(s) | 365, 572 |
| BceAI | ACGGC | 2 cut(s) | 171, 333 |
| BcgI | CGANNNNNNTGC | 3 cut(s) | 5, 36, 39 |
| BciT130I | CCWGG | 1 cut(s) | 336 |
| BfaI | CTAG | 3 cut(s) | 27, 765, 769 |
| BfmI | CTRYAG | 1 cut(s) | 167 |
| BfoI | RGCGCY | 3 cut(s) | 93, 161, 765 |
| BfuAI | ACCTGC | 2 cut(s) | 375, 659 |
| BglII | AGATCT | 1 cut(s) | 163 |
| BisI | GCNGC | 3 cut(s) | 24, 321, 559 |
| BlsI | GCNGC | 3 cut(s) | 25, 322, 560 |
| Bme1390I | CCNGG | 1 cut(s) | 336 |
| Bme18I | GGWCC | 2 cut(s) | 193, 294 |
| BmgBI | CACGTC | 1 cut(s) | 131 |
| BmgT120I | GGNCC | 2 cut(s) | 193, 294 |
| BmiI | GGNNCC | 6 cut(s) | 159, 194, 209, 271, 700, 752 |
| BmrFI | CCNGG | 1 cut(s) | 336 |
| BmrI | ACTGGG | 1 cut(s) | 506 |
| BmtI | GCTAGC | 1 cut(s) | 768 |
| BmuI | ACTGGG | 1 cut(s) | 506 |
| BpiI | GAAGAC | 1 cut(s) | 239 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 500, 532 |
| BpuEI | CTTGAG | 2 cut(s) | 96, 362 |
| BsaHI | GRCGYC | 1 cut(s) | 158 |
| BsaJI | CCNNGG | 3 cut(s) | 297, 335, 712 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 266, 490, 747 |
| Bse1I | ACTGG | 3 cut(s) | 48, 495, 501 |
| Bse3DI | GCAATG | 2 cut(s) | 686, 741 |
| BseBI | CCWGG | 1 cut(s) | 336 |
| BseDI | CCNNGG | 3 cut(s) | 297, 335, 712 |
| BseGI | GGATG | 2 cut(s) | 406, 583 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 266, 490, 747 |
| BseMI | GCAATG | 2 cut(s) | 686, 741 |
| BseNI | ACTGG | 3 cut(s) | 48, 495, 501 |
| BseSI | GKGCMC | 1 cut(s) | 317 |
| BseXI | GCAGC | 3 cut(s) | 10, 307, 570 |
| BsgI | GTGCAG | 1 cut(s) | 740 |
| Bsh1285I | CGRYCG | 2 cut(s) | 12, 378 |
| BshFI | GGCC | 2 cut(s) | 5, 419 |
| BshNI | GGYRCC | 1 cut(s) | 157 |
| BsiEI | CGRYCG | 2 cut(s) | 12, 378 |
| BsiHKAI | GWGCWC | 1 cut(s) | 317 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 266, 490, 747 |
| BsmI | GAATGC | 1 cut(s) | 205 |
| BsnI | GGCC | 2 cut(s) | 5, 419 |
| Bsp1286I | GDGCHC | 2 cut(s) | 110, 317 |
| Bsp143I | GATC | 4 cut(s) | 9, 163, 375, 622 |
| BspACI | CCGC | 2 cut(s) | 103, 693 |
| BspANI | GGCC | 2 cut(s) | 5, 419 |
| BspLI | GGNNCC | 6 cut(s) | 159, 194, 209, 271, 700, 752 |
| BspMI | ACCTGC | 2 cut(s) | 375, 659 |
| BspOI | GCTAGC | 1 cut(s) | 768 |
| BspPI | GGATC | 1 cut(s) | 617 |
| BspT107I | GGYRCC | 1 cut(s) | 157 |
| BsrDI | GCAATG | 2 cut(s) | 686, 741 |
| BsrI | ACTGG | 3 cut(s) | 48, 495, 501 |
| BssECI | CCNNGG | 3 cut(s) | 297, 335, 712 |
| BssMI | GATC | 4 cut(s) | 9, 163, 375, 622 |
| BssNI | GRCGYC | 1 cut(s) | 158 |
| BssT1I | CCWWGG | 1 cut(s) | 712 |
| Bst2UI | CCWGG | 1 cut(s) | 336 |
| Bst4CI | ACNGT | 2 cut(s) | 298, 644 |
| BstACI | GRCGYC | 1 cut(s) | 158 |
| BstC8I | GCNNGC | 5 cut(s) | 313, 382, 556, 719, 766 |
| BstDEI | CTNAG | 1 cut(s) | 78 |
| BstDSI | CCRYGG | 1 cut(s) | 297 |
| BstF5I | GGATG | 2 cut(s) | 406, 583 |
| BstH2I | RGCGCY | 3 cut(s) | 93, 161, 765 |
| BstHHI | GCGC | 5 cut(s) | 92, 160, 186, 448, 764 |
| BstKTI | GATC | 4 cut(s) | 12, 166, 378, 625 |
| BstMBI | GATC | 4 cut(s) | 9, 163, 375, 622 |
| BstMCI | CGRYCG | 2 cut(s) | 12, 378 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 32, 317, 377, 555, 714 |
| BstNI | CCWGG | 1 cut(s) | 336 |
| BstSCI | CCNGG | 1 cut(s) | 334 |
| BstSFI | CTRYAG | 1 cut(s) | 167 |
| BstSLI | GKGCMC | 1 cut(s) | 317 |
| BstV1I | GCAGC | 3 cut(s) | 10, 307, 570 |
| BstV2I | GAAGAC | 1 cut(s) | 239 |
| BstX2I | RGATCY | 1 cut(s) | 163 |
| BstYI | RGATCY | 1 cut(s) | 163 |
| BsuRI | GGCC | 2 cut(s) | 5, 419 |
| BtgI | CCRYGG | 1 cut(s) | 297 |
| BtgZI | GCGATG | 2 cut(s) | 72, 352 |
| BtrI | CACGTC | 1 cut(s) | 131 |
| BtsCI | GGATG | 2 cut(s) | 406, 583 |
| BtsI | GCAGTG | 1 cut(s) | 544 |
| BtsIMutI | CAGTG | 4 cut(s) | 41, 494, 544, 618 |
| BveI | ACCTGC | 2 cut(s) | 375, 659 |
| Cac8I | GCNNGC | 5 cut(s) | 313, 382, 556, 719, 766 |
| CfoI | GCGC | 5 cut(s) | 92, 160, 186, 448, 764 |
| Cfr13I | GGNCC | 2 cut(s) | 193, 294 |
| Csp6I | GTAC | 1 cut(s) | 302 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 615, 650 |
| CviAII | CATG | 4 cut(s) | 204, 440, 519, 572 |
| CviQI | GTAC | 1 cut(s) | 302 |
| DdeI | CTNAG | 1 cut(s) | 78 |
| DinI | GGCGCC | 1 cut(s) | 159 |
| DpnI | GATC | 4 cut(s) | 11, 165, 377, 624 |
| DpnII | GATC | 4 cut(s) | 9, 163, 375, 622 |
| DraIII | CACNNNGTG | 1 cut(s) | 646 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eco130I | CCWWGG | 1 cut(s) | 712 |
| Eco24I | GRGCYC | 1 cut(s) | 110 |
| Eco47I | GGWCC | 2 cut(s) | 193, 294 |
| EcoO109I | RGGNCCY | 1 cut(s) | 193 |
| EcoRII | CCWGG | 1 cut(s) | 334 |
| EcoT14I | CCWWGG | 1 cut(s) | 712 |
| EcoT22I | ATGCAT | 1 cut(s) | 205 |
| EcoT38I | GRGCYC | 1 cut(s) | 110 |
| EgeI | GGCGCC | 1 cut(s) | 159 |
| EheI | GGCGCC | 1 cut(s) | 159 |
| ErhI | CCWWGG | 1 cut(s) | 712 |
| FaeI | CATG | 4 cut(s) | 207, 443, 522, 575 |
| FaiI | YATR | 8 cut(s) | 205, 329, 422, 441, 450, 520, 573, 586 |
| FatI | CATG | 4 cut(s) | 203, 439, 518, 571 |
| FauI | CCCGC | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 3 cut(s) | 24, 321, 559 |
| FokI | GGATG | 2 cut(s) | 413, 590 |
| FriOI | GRGCYC | 1 cut(s) | 110 |
| Fsp4HI | GCNGC | 3 cut(s) | 24, 321, 559 |
| FspBI | CTAG | 3 cut(s) | 27, 765, 769 |
| GlaI | GCGC | 5 cut(s) | 91, 159, 185, 447, 763 |
| GluI | GCNGC | 3 cut(s) | 24, 321, 559 |
| HaeII | RGCGCY | 3 cut(s) | 93, 161, 765 |
| HaeIII | GGCC | 2 cut(s) | 5, 419 |
| HhaI | GCGC | 5 cut(s) | 92, 160, 186, 448, 764 |
| Hin1I | GRCGYC | 1 cut(s) | 158 |
| Hin1II | CATG | 4 cut(s) | 207, 443, 522, 575 |
| Hin6I | GCGC | 5 cut(s) | 90, 158, 184, 446, 762 |
| HinP1I | GCGC | 5 cut(s) | 90, 158, 184, 446, 762 |
| HincII | GTYRAC | 1 cut(s) | 474 |
| HindII | GTYRAC | 1 cut(s) | 474 |
| HindIII | AAGCTT | 2 cut(s) | 407, 552 |
| HinfI | GANTC | 2 cut(s) | 139, 501 |
| HphI | GGTGA | 2 cut(s) | 437, 682 |
| Hpy166II | GTNNAC | 4 cut(s) | 70, 294, 315, 474 |
| Hpy188I | TCNGA | 3 cut(s) | 138, 144, 537 |
| Hpy188III | TCNNGA | 3 cut(s) | 196, 222, 349 |
| Hpy8I | GTNNAC | 4 cut(s) | 70, 294, 315, 474 |
| Hpy99I | CGWCG | 1 cut(s) | 17 |
| HpyAV | CCTTC | 4 cut(s) | 221, 263, 294, 475 |
| HpyCH4III | ACNGT | 2 cut(s) | 298, 644 |
| HpyCH4IV | ACGT | 1 cut(s) | 130 |
| HpyCH4V | TGCA | 8 cut(s) | 203, 315, 558, 668, 679, 684, 721, 746 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 32, 317, 377, 555, 714 |
| HpyF3I | CTNAG | 1 cut(s) | 78 |
| HpySE526I | ACGT | 1 cut(s) | 130 |
| Hsp92I | GRCGYC | 1 cut(s) | 158 |
| Hsp92II | CATG | 4 cut(s) | 207, 443, 522, 575 |
| HspAI | GCGC | 5 cut(s) | 90, 158, 184, 446, 762 |
| KasI | GGCGCC | 1 cut(s) | 157 |
| Kzo9I | GATC | 4 cut(s) | 9, 163, 375, 622 |
| LmnI | GCTCC | 2 cut(s) | 177, 698 |
| Lsp1109I | GCAGC | 3 cut(s) | 10, 307, 570 |
| MaeI | CTAG | 3 cut(s) | 27, 765, 769 |
| MaeII | ACGT | 1 cut(s) | 130 |
| MalI | GATC | 4 cut(s) | 11, 165, 377, 624 |
| MboI | GATC | 4 cut(s) | 9, 163, 375, 622 |
| MboII | GAAGA | 1 cut(s) | 239 |
| MflI | RGATCY | 1 cut(s) | 163 |
| MhlI | GDGCHC | 2 cut(s) | 110, 317 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| Mly113I | GGCGCC | 1 cut(s) | 158 |
| MlyI | GAGTC | 1 cut(s) | 133 |
| MnlI | CCTC | 6 cut(s) | 46, 144, 229, 337, 502, 600 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| Mph1103I | ATGCAT | 1 cut(s) | 205 |
| MroXI | GAANNNNTTC | 2 cut(s) | 149, 464 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 1 cut(s) | 180 |
| MslI | CAYNNNNRTG | 1 cut(s) | 576 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspA1I | CMGCKG | 1 cut(s) | 103 |
| MspR9I | CCNGG | 1 cut(s) | 336 |
| Mva1269I | GAATGC | 1 cut(s) | 205 |
| MvaI | CCWGG | 1 cut(s) | 336 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 32, 317, 377, 555, 714 |
| NarI | GGCGCC | 1 cut(s) | 158 |
| NdeII | GATC | 4 cut(s) | 9, 163, 375, 622 |
| NheI | GCTAGC | 1 cut(s) | 764 |
| NlaIII | CATG | 4 cut(s) | 207, 443, 522, 575 |
| NlaIV | GGNNCC | 6 cut(s) | 159, 194, 209, 271, 700, 752 |
| NsiI | ATGCAT | 1 cut(s) | 205 |
| PctI | GAATGC | 1 cut(s) | 205 |
| PdmI | GAANNNNTTC | 2 cut(s) | 149, 464 |
| PfeI | GAWTC | 1 cut(s) | 501 |
| PkrI | GCNGC | 3 cut(s) | 25, 322, 560 |
| Ple19I | CGATCG | 2 cut(s) | 12, 378 |
| PleI | GAGTC | 1 cut(s) | 133 |
| PluTI | GGCGCC | 1 cut(s) | 161 |
| PpsI | GAGTC | 1 cut(s) | 133 |
| PpuMI | RGGWCCY | 1 cut(s) | 193 |
| Psp5II | RGGWCCY | 1 cut(s) | 193 |
| Psp6I | CCWGG | 1 cut(s) | 334 |
| PspGI | CCWGG | 1 cut(s) | 334 |
| PspN4I | GGNNCC | 6 cut(s) | 159, 194, 209, 271, 700, 752 |
| PspPI | GGNCC | 2 cut(s) | 193, 294 |
| PspPPI | RGGWCCY | 1 cut(s) | 193 |
| PsuI | RGATCY | 1 cut(s) | 163 |
| PvuI | CGATCG | 2 cut(s) | 12, 378 |
| RsaI | GTAC | 1 cut(s) | 303 |
| RsaNI | GTAC | 1 cut(s) | 302 |
| RseI | CAYNNNNRTG | 1 cut(s) | 576 |
| SaqAI | TTAA | 1 cut(s) | 180 |
| SatI | GCNGC | 3 cut(s) | 24, 321, 559 |
| Sau3AI | GATC | 4 cut(s) | 9, 163, 375, 622 |
| Sau96I | GGNCC | 2 cut(s) | 193, 294 |
| SchI | GAGTC | 1 cut(s) | 133 |
| ScrFI | CCNGG | 1 cut(s) | 336 |
| SduI | GDGCHC | 2 cut(s) | 110, 317 |
| SfcI | CTRYAG | 1 cut(s) | 167 |
| SfoI | GGCGCC | 1 cut(s) | 159 |
| SinI | GGWCC | 2 cut(s) | 193, 294 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 576 |
| SmlI | CTYRAG | 2 cut(s) | 111, 341 |
| SmoI | CTYRAG | 2 cut(s) | 111, 341 |
| SsiI | CCGC | 2 cut(s) | 103, 693 |
| SspDI | GGCGCC | 1 cut(s) | 157 |
| SspI | AATATT | 1 cut(s) | 454 |
| SspMI | CTAG | 3 cut(s) | 27, 765, 769 |
| StyD4I | CCNGG | 1 cut(s) | 334 |
| StyI | CCWWGG | 1 cut(s) | 712 |
| TaaI | ACNGT | 2 cut(s) | 298, 644 |
| TaiI | ACGT | 1 cut(s) | 133 |
| TaqI | TCGA | 4 cut(s) | 12, 256, 374, 601 |
| TfiI | GAWTC | 1 cut(s) | 501 |
| Tru1I | TTAA | 1 cut(s) | 180 |
| Tru9I | TTAA | 1 cut(s) | 180 |
| TscAI | CASTG | 4 cut(s) | 48, 501, 551, 625 |
| TseI | GCWGC | 3 cut(s) | 23, 320, 558 |
| TspDTI | ATGAA | 3 cut(s) | 428, 560, 624 |
| TspRI | CASTG | 4 cut(s) | 48, 501, 551, 625 |
| VneI | GTGCAC | 1 cut(s) | 313 |
| VpaK11BI | GGWCC | 2 cut(s) | 193, 294 |
| XmnI | GAANNNNTTC | 2 cut(s) | 149, 464 |
| XspI | CTAG | 3 cut(s) | 27, 765, 769 |
| Zsp2I | ATGCAT | 1 cut(s) | 205 |