Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 481 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 473 |
| AciI | CCGC | 2 cut(s) | 33, 48 |
| AclWI | GGATC | 1 cut(s) | 516 |
| AcuI | CTGAAG | 1 cut(s) | 331 |
| AfaI | GTAC | 1 cut(s) | 333 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 230, 473 |
| AflII | CTTAAG | 1 cut(s) | 59 |
| AgsI | TTSAA | 1 cut(s) | 442 |
| AluBI | AGCT | 2 cut(s) | 168, 365 |
| AluI | AGCT | 2 cut(s) | 168, 365 |
| Alw21I | GWGCWC | 1 cut(s) | 238 |
| Alw26I | GTCTC | 1 cut(s) | 126 |
| AlwI | GGATC | 1 cut(s) | 516 |
| AoxI | GGCC | 1 cut(s) | 269 |
| ApeKI | GCWGC | 1 cut(s) | 296 |
| AseI | ATTAAT | 1 cut(s) | 419 |
| AspS9I | GGNCC | 1 cut(s) | 428 |
| AsuHPI | GGTGA | 2 cut(s) | 151, 523 |
| AvaII | GGWCC | 1 cut(s) | 428 |
| BanI | GGYRCC | 1 cut(s) | 481 |
| Bbv12I | GWGCWC | 1 cut(s) | 238 |
| BbvI | GCAGC | 1 cut(s) | 283 |
| BccI | CCATC | 3 cut(s) | 221, 467, 496 |
| BceAI | ACGGC | 2 cut(s) | 73, 84 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 302, 336 |
| BcoDI | GTCTC | 1 cut(s) | 126 |
| BfmI | CTRYAG | 1 cut(s) | 495 |
| BfrI | CTTAAG | 1 cut(s) | 59 |
| BisI | GCNGC | 3 cut(s) | 34, 48, 297 |
| BlsI | GCNGC | 3 cut(s) | 35, 49, 298 |
| Bme18I | GGWCC | 1 cut(s) | 428 |
| BmgT120I | GGNCC | 1 cut(s) | 428 |
| BmiI | GGNNCC | 1 cut(s) | 483 |
| BmsI | GCATC | 2 cut(s) | 125, 537 |
| Bpu10I | CCTNAGC | 1 cut(s) | 29 |
| BsaAI | YACGTR | 1 cut(s) | 308 |
| BsaJI | CCNNGG | 1 cut(s) | 431 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 230, 473 |
| Bse1I | ACTGG | 1 cut(s) | 186 |
| BseDI | CCNNGG | 1 cut(s) | 431 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 230, 473 |
| BseNI | ACTGG | 1 cut(s) | 186 |
| BseRI | GAGGAG | 1 cut(s) | 414 |
| BseXI | GCAGC | 1 cut(s) | 283 |
| BshFI | GGCC | 1 cut(s) | 271 |
| BshNI | GGYRCC | 1 cut(s) | 481 |
| BsiHKAI | GWGCWC | 1 cut(s) | 238 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 230, 473 |
| BsmAI | GTCTC | 1 cut(s) | 126 |
| BsmBI | CGTCTC | 1 cut(s) | 126 |
| BsnI | GGCC | 1 cut(s) | 271 |
| Bsp1286I | GDGCHC | 1 cut(s) | 238 |
| Bsp143I | GATC | 3 cut(s) | 301, 391, 508 |
| BspACI | CCGC | 2 cut(s) | 33, 48 |
| BspANI | GGCC | 1 cut(s) | 271 |
| BspLI | GGNNCC | 1 cut(s) | 483 |
| BspPI | GGATC | 1 cut(s) | 516 |
| BspT107I | GGYRCC | 1 cut(s) | 481 |
| BspTI | CTTAAG | 1 cut(s) | 59 |
| BsrI | ACTGG | 1 cut(s) | 186 |
| BssECI | CCNNGG | 1 cut(s) | 431 |
| BssMI | GATC | 3 cut(s) | 301, 391, 508 |
| Bst4CI | ACNGT | 1 cut(s) | 432 |
| Bst6I | CTCTTC | 2 cut(s) | 140, 220 |
| BstAFI | CTTAAG | 1 cut(s) | 59 |
| BstBAI | YACGTR | 1 cut(s) | 308 |
| BstDEI | CTNAG | 1 cut(s) | 29 |
| BstDSI | CCRYGG | 1 cut(s) | 431 |
| BstKTI | GATC | 3 cut(s) | 304, 394, 511 |
| BstMAI | GTCTC | 1 cut(s) | 126 |
| BstMBI | GATC | 3 cut(s) | 301, 391, 508 |
| BstSFI | CTRYAG | 1 cut(s) | 495 |
| BstSNI | TACGTA | 1 cut(s) | 308 |
| BstV1I | GCAGC | 1 cut(s) | 283 |
| BstX2I | RGATCY | 1 cut(s) | 508 |
| BstYI | RGATCY | 1 cut(s) | 508 |
| BsuRI | GGCC | 1 cut(s) | 271 |
| BtgI | CCRYGG | 1 cut(s) | 431 |
| BtsIMutI | CAGTG | 1 cut(s) | 179 |
| Cfr13I | GGNCC | 1 cut(s) | 428 |
| Csp6I | GTAC | 1 cut(s) | 332 |
| CviAII | CATG | 2 cut(s) | 139, 220 |
| CviJI | RGCY | 8 cut(s) | 36, 168, 185, 271, 345, 365, 449, 466 |
| CviKI_1 | RGCY | 8 cut(s) | 36, 168, 185, 271, 345, 365, 449, 466 |
| CviQI | GTAC | 1 cut(s) | 332 |
| DdeI | CTNAG | 1 cut(s) | 29 |
| DpnI | GATC | 3 cut(s) | 303, 393, 510 |
| DpnII | GATC | 3 cut(s) | 301, 391, 508 |
| Eam1104I | CTCTTC | 2 cut(s) | 140, 220 |
| EarI | CTCTTC | 2 cut(s) | 140, 220 |
| Eco105I | TACGTA | 1 cut(s) | 308 |
| Eco147I | AGGCCT | 1 cut(s) | 271 |
| Eco32I | GATATC | 1 cut(s) | 211 |
| Eco47I | GGWCC | 1 cut(s) | 428 |
| Eco57I | CTGAAG | 1 cut(s) | 331 |
| EcoRV | GATATC | 1 cut(s) | 211 |
| Esp3I | CGTCTC | 1 cut(s) | 126 |
| FaeI | CATG | 2 cut(s) | 142, 223 |
| FaiI | YATR | 7 cut(s) | 140, 221, 256, 469, 471, 539, 550 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 157, 189 |
| FatI | CATG | 2 cut(s) | 138, 219 |
| FauNDI | CATATG | 1 cut(s) | 469 |
| Fnu4HI | GCNGC | 3 cut(s) | 34, 48, 297 |
| Fsp4HI | GCNGC | 3 cut(s) | 34, 48, 297 |
| GluI | GCNGC | 3 cut(s) | 34, 48, 297 |
| HaeIII | GGCC | 1 cut(s) | 271 |
| Hin1II | CATG | 2 cut(s) | 142, 223 |
| HindIII | AAGCTT | 1 cut(s) | 166 |
| HinfI | GANTC | 2 cut(s) | 152, 277 |
| HphI | GGTGA | 2 cut(s) | 151, 523 |
| Hpy166II | GTNNAC | 1 cut(s) | 428 |
| Hpy188I | TCNGA | 2 cut(s) | 276, 369 |
| Hpy8I | GTNNAC | 1 cut(s) | 428 |
| Hpy99I | CGWCG | 1 cut(s) | 324 |
| HpyAV | CCTTC | 1 cut(s) | 85 |
| HpyCH4III | ACNGT | 1 cut(s) | 432 |
| HpyCH4IV | ACGT | 1 cut(s) | 307 |
| HpyCH4V | TGCA | 2 cut(s) | 116, 490 |
| HpyF3I | CTNAG | 1 cut(s) | 29 |
| HpySE526I | ACGT | 1 cut(s) | 307 |
| Hsp92II | CATG | 2 cut(s) | 142, 223 |
| Kzo9I | GATC | 3 cut(s) | 301, 391, 508 |
| LmnI | GCTCC | 2 cut(s) | 233, 370 |
| LpnPI | CCDG | 2 cut(s) | 167, 237 |
| Lsp1109I | GCAGC | 1 cut(s) | 283 |
| LweI | GCATC | 2 cut(s) | 125, 537 |
| MaeII | ACGT | 1 cut(s) | 307 |
| MaeIII | GTNAC | 1 cut(s) | 157 |
| MalI | GATC | 3 cut(s) | 303, 393, 510 |
| MboI | GATC | 3 cut(s) | 301, 391, 508 |
| MboII | GAAGA | 3 cut(s) | 127, 207, 386 |
| MflI | RGATCY | 1 cut(s) | 508 |
| MhlI | GDGCHC | 1 cut(s) | 238 |
| MlyI | GAGTC | 1 cut(s) | 271 |
| MnlI | CCTC | 6 cut(s) | 187, 193, 282, 392, 495, 499 |
| MseI | TTAA | 2 cut(s) | 60, 419 |
| MslI | CAYNNNNRTG | 1 cut(s) | 536 |
| MspCI | CTTAAG | 1 cut(s) | 59 |
| NdeI | CATATG | 1 cut(s) | 469 |
| NdeII | GATC | 3 cut(s) | 301, 391, 508 |
| NlaIII | CATG | 2 cut(s) | 142, 223 |
| NlaIV | GGNNCC | 1 cut(s) | 483 |
| NmuCI | GTSAC | 1 cut(s) | 157 |
| PceI | AGGCCT | 1 cut(s) | 271 |
| PfeI | GAWTC | 1 cut(s) | 152 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 473 |
| PkrI | GCNGC | 3 cut(s) | 35, 49, 298 |
| PleI | GAGTC | 1 cut(s) | 271 |
| PpsI | GAGTC | 1 cut(s) | 271 |
| Ppu21I | YACGTR | 1 cut(s) | 308 |
| PshBI | ATTAAT | 1 cut(s) | 419 |
| PspN4I | GGNNCC | 1 cut(s) | 483 |
| PspPI | GGNCC | 1 cut(s) | 428 |
| PsuI | RGATCY | 1 cut(s) | 508 |
| RsaI | GTAC | 1 cut(s) | 333 |
| RsaNI | GTAC | 1 cut(s) | 332 |
| RseI | CAYNNNNRTG | 1 cut(s) | 536 |
| SaqAI | TTAA | 2 cut(s) | 60, 419 |
| SatI | GCNGC | 3 cut(s) | 34, 48, 297 |
| Sau3AI | GATC | 3 cut(s) | 301, 391, 508 |
| Sau96I | GGNCC | 1 cut(s) | 428 |
| SchI | GAGTC | 1 cut(s) | 271 |
| SduI | GDGCHC | 1 cut(s) | 238 |
| SetI | ASST | 9 cut(s) | 60, 77, 102, 170, 204, 310, 367, 417, 487 |
| SfaNI | GCATC | 2 cut(s) | 125, 537 |
| SfcI | CTRYAG | 1 cut(s) | 495 |
| SinI | GGWCC | 1 cut(s) | 428 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 536 |
| SmlI | CTYRAG | 1 cut(s) | 59 |
| SmoI | CTYRAG | 1 cut(s) | 59 |
| SnaBI | TACGTA | 1 cut(s) | 308 |
| SseBI | AGGCCT | 1 cut(s) | 271 |
| SsiI | CCGC | 2 cut(s) | 33, 48 |
| SspI | AATATT | 1 cut(s) | 373 |
| StuI | AGGCCT | 1 cut(s) | 271 |
| TaaI | ACNGT | 1 cut(s) | 432 |
| TaiI | ACGT | 1 cut(s) | 310 |
| TaqI | TCGA | 2 cut(s) | 143, 397 |
| TauI | GCSGC | 2 cut(s) | 36, 50 |
| TfiI | GAWTC | 1 cut(s) | 152 |
| Tru1I | TTAA | 2 cut(s) | 60, 419 |
| Tru9I | TTAA | 2 cut(s) | 60, 419 |
| TscAI | CASTG | 1 cut(s) | 186 |
| TseFI | GTSAC | 1 cut(s) | 157 |
| TseI | GCWGC | 1 cut(s) | 296 |
| Tsp45I | GTSAC | 1 cut(s) | 157 |
| TspDTI | ATGAA | 1 cut(s) | 127 |
| TspRI | CASTG | 1 cut(s) | 186 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 473 |
| Vha464I | CTTAAG | 1 cut(s) | 59 |
| VpaK11BI | GGWCC | 1 cut(s) | 428 |
| VspI | ATTAAT | 1 cut(s) | 419 |