No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 767 |
| Acc36I | ACCTGC | 2 cut(s) | 376, 780 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 274 |
| AciI | CCGC | 2 cut(s) | 452, 706 |
| AclI | AACGTT | 1 cut(s) | 784 |
| AclWI | GGATC | 1 cut(s) | 848 |
| AcsI | RAATTY | 2 cut(s) | 122, 582 |
| AfaI | GTAC | 1 cut(s) | 639 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 274 |
| AgsI | TTSAA | 4 cut(s) | 113, 122, 587, 644 |
| AluBI | AGCT | 5 cut(s) | 9, 94, 309, 337, 652 |
| AluI | AGCT | 5 cut(s) | 9, 94, 309, 337, 652 |
| Alw26I | GTCTC | 2 cut(s) | 798, 816 |
| AlwI | GGATC | 1 cut(s) | 848 |
| Ama87I | CYCGRG | 1 cut(s) | 771 |
| AoxI | GGCC | 1 cut(s) | 17 |
| ApoI | RAATTY | 2 cut(s) | 122, 582 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 583, 615 |
| AspS9I | GGNCC | 2 cut(s) | 356, 608 |
| AsuHPI | GGTGA | 2 cut(s) | 37, 641 |
| AvaI | CYCGRG | 1 cut(s) | 771 |
| AvaII | GGWCC | 2 cut(s) | 356, 608 |
| BbsI | GAAGAC | 1 cut(s) | 521 |
| BccI | CCATC | 4 cut(s) | 401, 599, 703, 805 |
| BcoDI | GTCTC | 2 cut(s) | 798, 816 |
| BfaI | CTAG | 1 cut(s) | 438 |
| BfuAI | ACCTGC | 2 cut(s) | 376, 780 |
| BisI | GCNGC | 1 cut(s) | 452 |
| BlpI | GCTNAGC | 1 cut(s) | 702 |
| BlsI | GCNGC | 1 cut(s) | 453 |
| Bme18I | GGWCC | 2 cut(s) | 356, 608 |
| BmeT110I | CYCGRG | 1 cut(s) | 771 |
| BmgT120I | GGNCC | 2 cut(s) | 356, 608 |
| BmiI | GGNNCC | 1 cut(s) | 358 |
| BmsI | GCATC | 2 cut(s) | 226, 438 |
| BpiI | GAAGAC | 1 cut(s) | 521 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 702 |
| BpuEI | CTTGAG | 1 cut(s) | 504 |
| BsaI | GGTCTC | 1 cut(s) | 798 |
| BsaJI | CCNNGG | 1 cut(s) | 662 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 230, 260 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 274 |
| Bse1I | ACTGG | 4 cut(s) | 27, 106, 508, 631 |
| BseDI | CCNNGG | 1 cut(s) | 662 |
| BseGI | GGATG | 3 cut(s) | 610, 714, 760 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 274 |
| BseMII | CTCAG | 2 cut(s) | 68, 639 |
| BseNI | ACTGG | 4 cut(s) | 27, 106, 508, 631 |
| BseRI | GAGGAG | 1 cut(s) | 572 |
| BshFI | GGCC | 1 cut(s) | 19 |
| BsiHKCI | CYCGRG | 1 cut(s) | 771 |
| BslFI | GGGAC | 3 cut(s) | 70, 342, 745 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 274 |
| BsmAI | GTCTC | 2 cut(s) | 798, 816 |
| BsmFI | GGGAC | 3 cut(s) | 70, 342, 745 |
| BsnI | GGCC | 1 cut(s) | 19 |
| Bso31I | GGTCTC | 1 cut(s) | 798 |
| BsoBI | CYCGRG | 1 cut(s) | 771 |
| Bsp143I | GATC | 1 cut(s) | 840 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 702 |
| BspACI | CCGC | 2 cut(s) | 452, 706 |
| BspANI | GGCC | 1 cut(s) | 19 |
| BspCNI | CTCAG | 2 cut(s) | 69, 640 |
| BspLI | GGNNCC | 1 cut(s) | 358 |
| BspMI | ACCTGC | 2 cut(s) | 376, 780 |
| BspPI | GGATC | 1 cut(s) | 848 |
| BspTNI | GGTCTC | 1 cut(s) | 798 |
| BsrI | ACTGG | 4 cut(s) | 27, 106, 508, 631 |
| BssECI | CCNNGG | 1 cut(s) | 662 |
| BssMI | GATC | 1 cut(s) | 840 |
| BssT1I | CCWWGG | 1 cut(s) | 662 |
| Bst4CI | ACNGT | 3 cut(s) | 214, 409, 810 |
| BstC8I | GCNNGC | 2 cut(s) | 96, 654 |
| BstDEI | CTNAG | 3 cut(s) | 77, 648, 702 |
| BstF5I | GGATG | 3 cut(s) | 610, 714, 760 |
| BstKTI | GATC | 1 cut(s) | 843 |
| BstMAI | GTCTC | 2 cut(s) | 798, 816 |
| BstMBI | GATC | 1 cut(s) | 840 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 214 |
| BstV2I | GAAGAC | 1 cut(s) | 521 |
| BstX2I | RGATCY | 1 cut(s) | 840 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 553 |
| BstYI | RGATCY | 1 cut(s) | 840 |
| BsuRI | GGCC | 1 cut(s) | 19 |
| BtsCI | GGATG | 3 cut(s) | 610, 714, 760 |
| BtsIMutI | CAGTG | 2 cut(s) | 20, 219 |
| BveI | ACCTGC | 2 cut(s) | 376, 780 |
| Cac8I | GCNNGC | 2 cut(s) | 96, 654 |
| Cfr13I | GGNCC | 2 cut(s) | 356, 608 |
| Csp6I | GTAC | 1 cut(s) | 638 |
| CviAII | CATG | 6 cut(s) | 49, 193, 314, 574, 697, 837 |
| CviQI | GTAC | 1 cut(s) | 638 |
| DdeI | CTNAG | 3 cut(s) | 77, 648, 702 |
| DpnI | GATC | 1 cut(s) | 842 |
| DpnII | GATC | 1 cut(s) | 840 |
| Eco130I | CCWWGG | 1 cut(s) | 662 |
| Eco31I | GGTCTC | 1 cut(s) | 798 |
| Eco47I | GGWCC | 2 cut(s) | 356, 608 |
| Eco88I | CYCGRG | 1 cut(s) | 771 |
| EcoRI | GAATTC | 1 cut(s) | 122 |
| EcoT14I | CCWWGG | 1 cut(s) | 662 |
| EcoT22I | ATGCAT | 1 cut(s) | 194 |
| ErhI | CCWWGG | 1 cut(s) | 662 |
| FaeI | CATG | 6 cut(s) | 52, 196, 317, 577, 700, 840 |
| FaqI | GGGAC | 3 cut(s) | 70, 342, 745 |
| FatI | CATG | 6 cut(s) | 48, 192, 313, 573, 696, 836 |
| FauNDI | CATATG | 1 cut(s) | 564 |
| Fnu4HI | GCNGC | 1 cut(s) | 452 |
| FokI | GGATG | 3 cut(s) | 617, 721, 767 |
| Fsp4HI | GCNGC | 1 cut(s) | 452 |
| FspBI | CTAG | 1 cut(s) | 438 |
| GluI | GCNGC | 1 cut(s) | 452 |
| HaeIII | GGCC | 1 cut(s) | 19 |
| Hin1II | CATG | 6 cut(s) | 52, 196, 317, 577, 700, 840 |
| HincII | GTYRAC | 1 cut(s) | 57 |
| HindII | GTYRAC | 1 cut(s) | 57 |
| HindIII | AAGCTT | 1 cut(s) | 92 |
| HinfI | GANTC | 3 cut(s) | 136, 517, 644 |
| HphI | GGTGA | 2 cut(s) | 37, 641 |
| Hpy166II | GTNNAC | 2 cut(s) | 57, 638 |
| Hpy188I | TCNGA | 4 cut(s) | 78, 612, 649, 804 |
| Hpy188III | TCNNGA | 2 cut(s) | 268, 397 |
| Hpy8I | GTNNAC | 2 cut(s) | 57, 638 |
| HpyAV | CCTTC | 4 cut(s) | 229, 394, 415, 823 |
| HpyCH4III | ACNGT | 3 cut(s) | 214, 409, 810 |
| HpyCH4IV | ACGT | 1 cut(s) | 784 |
| HpyCH4V | TGCA | 8 cut(s) | 52, 162, 192, 217, 293, 573, 656, 789 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 214 |
| HpyF3I | CTNAG | 3 cut(s) | 77, 648, 702 |
| HpySE526I | ACGT | 1 cut(s) | 784 |
| Hsp92II | CATG | 6 cut(s) | 52, 196, 317, 577, 700, 840 |
| Kzo9I | GATC | 1 cut(s) | 840 |
| LmnI | GCTCC | 2 cut(s) | 342, 559 |
| LweI | GCATC | 2 cut(s) | 226, 438 |
| MaeI | CTAG | 1 cut(s) | 438 |
| MaeII | ACGT | 1 cut(s) | 784 |
| MaeIII | GTNAC | 2 cut(s) | 388, 589 |
| MalI | GATC | 1 cut(s) | 842 |
| MboI | GATC | 1 cut(s) | 840 |
| MboII | GAAGA | 2 cut(s) | 125, 526 |
| MflI | RGATCY | 1 cut(s) | 840 |
| MluCI | AATT | 5 cut(s) | 105, 117, 122, 157, 582 |
| MlyI | GAGTC | 1 cut(s) | 511 |
| MmeI | TCCRAC | 2 cut(s) | 228, 570 |
| MnlI | CCTC | 9 cut(s) | 72, 175, 351, 387, 418, 523, 550, 571, 745 |
| Mph1103I | ATGCAT | 1 cut(s) | 194 |
| MseI | TTAA | 2 cut(s) | 14, 279 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 214 |
| NdeI | CATATG | 1 cut(s) | 564 |
| NdeII | GATC | 1 cut(s) | 840 |
| NlaIII | CATG | 6 cut(s) | 52, 196, 317, 577, 700, 840 |
| NlaIV | GGNNCC | 1 cut(s) | 358 |
| NmuCI | GTSAC | 2 cut(s) | 388, 589 |
| NsiI | ATGCAT | 1 cut(s) | 194 |
| PaeR7I | CTCGAG | 1 cut(s) | 771 |
| PfeI | GAWTC | 2 cut(s) | 136, 644 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 274 |
| PkrI | GCNGC | 1 cut(s) | 453 |
| PleI | GAGTC | 1 cut(s) | 511 |
| PpsI | GAGTC | 1 cut(s) | 511 |
| PsiI | TTATAA | 1 cut(s) | 767 |
| Psp1406I | AACGTT | 1 cut(s) | 784 |
| PspN4I | GGNNCC | 1 cut(s) | 358 |
| PspPI | GGNCC | 2 cut(s) | 356, 608 |
| PspXI | VCTCGAGB | 1 cut(s) | 771 |
| PsuI | RGATCY | 1 cut(s) | 840 |
| RsaI | GTAC | 1 cut(s) | 639 |
| RsaNI | GTAC | 1 cut(s) | 638 |
| SaqAI | TTAA | 2 cut(s) | 14, 279 |
| SatI | GCNGC | 1 cut(s) | 452 |
| Sau3AI | GATC | 1 cut(s) | 840 |
| Sau96I | GGNCC | 2 cut(s) | 356, 608 |
| SchI | GAGTC | 1 cut(s) | 511 |
| SfaNI | GCATC | 2 cut(s) | 226, 438 |
| Sfr274I | CTCGAG | 1 cut(s) | 771 |
| SinI | GGWCC | 2 cut(s) | 356, 608 |
| SlaI | CTCGAG | 1 cut(s) | 771 |
| SmlI | CTYRAG | 2 cut(s) | 519, 771 |
| SmoI | CTYRAG | 2 cut(s) | 519, 771 |
| Sse9I | AATT | 5 cut(s) | 105, 117, 122, 157, 582 |
| SsiI | CCGC | 2 cut(s) | 452, 706 |
| SspMI | CTAG | 1 cut(s) | 438 |
| StyI | CCWWGG | 1 cut(s) | 662 |
| TaaI | ACNGT | 3 cut(s) | 214, 409, 810 |
| TaiI | ACGT | 1 cut(s) | 787 |
| TaqI | TCGA | 3 cut(s) | 379, 419, 772 |
| TaqII | GACCGA | 1 cut(s) | 608 |
| TasI | AATT | 5 cut(s) | 105, 117, 122, 157, 582 |
| TatI | WGTACW | 1 cut(s) | 637 |
| TauI | GCSGC | 1 cut(s) | 454 |
| TfiI | GAWTC | 2 cut(s) | 136, 644 |
| Tru1I | TTAA | 2 cut(s) | 14, 279 |
| Tru9I | TTAA | 2 cut(s) | 14, 279 |
| TscAI | CASTG | 2 cut(s) | 27, 219 |
| TseFI | GTSAC | 2 cut(s) | 388, 589 |
| Tsp45I | GTSAC | 2 cut(s) | 388, 589 |
| TspDTI | ATGAA | 3 cut(s) | 164, 302, 842 |
| TspGWI | ACGGA | 1 cut(s) | 194 |
| TspRI | CASTG | 2 cut(s) | 27, 219 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 274 |
| VpaK11BI | GGWCC | 2 cut(s) | 356, 608 |
| XapI | RAATTY | 2 cut(s) | 122, 582 |
| XhoI | CTCGAG | 1 cut(s) | 771 |
| XspI | CTAG | 1 cut(s) | 438 |
| Zsp2I | ATGCAT | 1 cut(s) | 194 |