Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 8 |
| AccB1I | GGYRCC | 1 cut(s) | 1045 |
| AccII | CGCG | 1 cut(s) | 820 |
| AciI | CCGC | 2 cut(s) | 404, 856 |
| AclI | AACGTT | 1 cut(s) | 773 |
| AclWI | GGATC | 2 cut(s) | 97, 341 |
| AcoI | YGGCCR | 1 cut(s) | 126 |
| AcsI | RAATTY | 2 cut(s) | 1070, 1083 |
| AcuI | CTGAAG | 2 cut(s) | 566, 1275 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AfaI | GTAC | 2 cut(s) | 226, 1065 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1142 |
| AflII | CTTAAG | 1 cut(s) | 607 |
| AgsI | TTSAA | 7 cut(s) | 85, 181, 304, 932, 1060, 1112, 1236 |
| AjiI | CACGTC | 1 cut(s) | 514 |
| AjnI | CCWGG | 3 cut(s) | 568, 1098, 1279 |
| AluBI | AGCT | 5 cut(s) | 311, 853, 1035, 1115, 1253 |
| AluI | AGCT | 5 cut(s) | 311, 853, 1035, 1115, 1253 |
| Alw26I | GTCTC | 3 cut(s) | 1016, 1109, 1310 |
| AlwI | GGATC | 2 cut(s) | 97, 341 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 59, 627 |
| AoxI | GGCC | 5 cut(s) | 126, 290, 958, 962, 1205 |
| ApeKI | GCWGC | 1 cut(s) | 695 |
| ApoI | RAATTY | 2 cut(s) | 1070, 1083 |
| AspLEI | GCGC | 3 cut(s) | 102, 820, 822 |
| AspS9I | GGNCC | 2 cut(s) | 290, 922 |
| AsuC2I | CCSGG | 2 cut(s) | 741, 1119 |
| AsuHPI | GGTGA | 4 cut(s) | 37, 961, 963, 1031 |
| AsuII | TTCGAA | 1 cut(s) | 704 |
| AsuNHI | GCTAGC | 1 cut(s) | 1031 |
| AvaII | GGWCC | 1 cut(s) | 922 |
| AxyI | CCTNAGG | 1 cut(s) | 1137 |
| BaeGI | GKGCMC | 1 cut(s) | 1050 |
| BalI | TGGCCA | 1 cut(s) | 128 |
| BanI | GGYRCC | 1 cut(s) | 1045 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 452, 484 |
| BbsI | GAAGAC | 2 cut(s) | 713, 888 |
| BbvI | GCAGC | 1 cut(s) | 682 |
| BccI | CCATC | 3 cut(s) | 194, 329, 639 |
| BceAI | ACGGC | 1 cut(s) | 277 |
| BciT130I | CCWGG | 3 cut(s) | 570, 1100, 1281 |
| BciVI | GTATCC | 1 cut(s) | 410 |
| BclI | TGATCA | 1 cut(s) | 1173 |
| BcnI | CCSGG | 2 cut(s) | 741, 1119 |
| BcoDI | GTCTC | 3 cut(s) | 1016, 1109, 1310 |
| BfaI | CTAG | 4 cut(s) | 413, 1032, 1293, 1299 |
| BfoI | RGCGCY | 1 cut(s) | 103 |
| BfrI | CTTAAG | 1 cut(s) | 607 |
| BfuI | GTATCC | 1 cut(s) | 410 |
| BglII | AGATCT | 1 cut(s) | 163 |
| BisI | GCNGC | 1 cut(s) | 696 |
| BlpI | GCTNAGC | 1 cut(s) | 1265 |
| BlsI | GCNGC | 1 cut(s) | 697 |
| Bme1390I | CCNGG | 5 cut(s) | 570, 741, 1100, 1119, 1281 |
| Bme18I | GGWCC | 1 cut(s) | 922 |
| BmgBI | CACGTC | 1 cut(s) | 514 |
| BmgT120I | GGNCC | 2 cut(s) | 290, 922 |
| BmiI | GGNNCC | 3 cut(s) | 205, 924, 1047 |
| BmrFI | CCNGG | 5 cut(s) | 570, 741, 1100, 1119, 1281 |
| BmrI | ACTGGG | 1 cut(s) | 806 |
| BmsI | GCATC | 3 cut(s) | 195, 661, 989 |
| BmtI | GCTAGC | 1 cut(s) | 1035 |
| BmuI | ACTGGG | 1 cut(s) | 806 |
| BpiI | GAAGAC | 2 cut(s) | 713, 888 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 86, 118 |
| BpmI | CTGGAG | 1 cut(s) | 591 |
| Bpu10I | CCTNAGC | 1 cut(s) | 49 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1265 |
| Bpu14I | TTCGAA | 1 cut(s) | 704 |
| BpuEI | CTTGAG | 2 cut(s) | 144, 907 |
| BpuMI | CCSGG | 2 cut(s) | 741, 1119 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaI | GGTCTC | 1 cut(s) | 1109 |
| BsaJI | CCNNGG | 7 cut(s) | 293, 649, 918, 945, 1098, 1099, 1279 |
| BsaWI | WCCGGW | 1 cut(s) | 313 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 443, 473 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1142 |
| Bse118I | RCCGGY | 1 cut(s) | 960 |
| Bse1I | ACTGG | 2 cut(s) | 699, 812 |
| Bse21I | CCTNAGG | 1 cut(s) | 1137 |
| BseBI | CCWGG | 3 cut(s) | 570, 1100, 1281 |
| BseDI | CCNNGG | 7 cut(s) | 293, 649, 918, 945, 1098, 1099, 1279 |
| BseGI | GGATG | 3 cut(s) | 38, 728, 980 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1142 |
| BseMII | CTCAG | 2 cut(s) | 125, 367 |
| BseNI | ACTGG | 2 cut(s) | 699, 812 |
| BsePI | GCGCGC | 1 cut(s) | 818 |
| BseSI | GKGCMC | 1 cut(s) | 1050 |
| BseXI | GCAGC | 1 cut(s) | 682 |
| Bsh1236I | CGCG | 1 cut(s) | 820 |
| BshFI | GGCC | 5 cut(s) | 128, 292, 960, 964, 1207 |
| BshNI | GGYRCC | 1 cut(s) | 1045 |
| BsiSI | CCGG | 4 cut(s) | 314, 741, 961, 1118 |
| BslFI | GGGAC | 2 cut(s) | 638, 908 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1142 |
| BsmAI | GTCTC | 3 cut(s) | 1016, 1109, 1310 |
| BsmFI | GGGAC | 2 cut(s) | 638, 908 |
| BsmI | GAATGC | 1 cut(s) | 357 |
| BsnI | GGCC | 5 cut(s) | 128, 292, 960, 964, 1207 |
| Bso31I | GGTCTC | 1 cut(s) | 1109 |
| Bsp119I | TTCGAA | 1 cut(s) | 704 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1050 |
| Bsp143I | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1265 |
| BspACI | CCGC | 2 cut(s) | 404, 856 |
| BspANI | GGCC | 5 cut(s) | 128, 292, 960, 964, 1207 |
| BspCNI | CTCAG | 2 cut(s) | 124, 366 |
| BspFNI | CGCG | 1 cut(s) | 820 |
| BspHI | TCATGA | 3 cut(s) | 432, 643, 843 |
| BspLI | GGNNCC | 3 cut(s) | 205, 924, 1047 |
| BspOI | GCTAGC | 1 cut(s) | 1035 |
| BspPI | GGATC | 2 cut(s) | 97, 341 |
| BspQI | GCTCTTC | 2 cut(s) | 380, 759 |
| BspT104I | TTCGAA | 1 cut(s) | 704 |
| BspT107I | GGYRCC | 1 cut(s) | 1045 |
| BspTI | CTTAAG | 1 cut(s) | 607 |
| BspTNI | GGTCTC | 1 cut(s) | 1109 |
| BsrFI | RCCGGY | 1 cut(s) | 960 |
| BsrI | ACTGG | 2 cut(s) | 699, 812 |
| BssAI | RCCGGY | 1 cut(s) | 960 |
| BssECI | CCNNGG | 7 cut(s) | 293, 649, 918, 945, 1098, 1099, 1279 |
| BssHII | GCGCGC | 1 cut(s) | 818 |
| BssMI | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 3 cut(s) | 649, 918, 945 |
| Bst2UI | CCWGG | 3 cut(s) | 570, 1100, 1281 |
| Bst4CI | ACNGT | 2 cut(s) | 150, 682 |
| Bst6I | CTCTTC | 4 cut(s) | 380, 542, 759, 1251 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstAFI | CTTAAG | 1 cut(s) | 607 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 627 |
| BstBI | TTCGAA | 1 cut(s) | 704 |
| BstC8I | GCNNGC | 6 cut(s) | 55, 408, 820, 860, 962, 1033 |
| BstDEI | CTNAG | 5 cut(s) | 49, 111, 353, 1137, 1265 |
| BstDSI | CCRYGG | 1 cut(s) | 293 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1140 |
| BstF5I | GGATG | 3 cut(s) | 38, 728, 980 |
| BstFNI | CGCG | 1 cut(s) | 820 |
| BstH2I | RGCGCY | 1 cut(s) | 103 |
| BstHHI | GCGC | 3 cut(s) | 102, 820, 822 |
| BstKTI | GATC | 6 cut(s) | 92, 166, 235, 256, 349, 1176 |
| BstMAI | GTCTC | 3 cut(s) | 1016, 1109, 1310 |
| BstMBI | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 32, 627, 859, 1275 |
| BstNI | CCWGG | 3 cut(s) | 570, 1100, 1281 |
| BstSCI | CCNGG | 5 cut(s) | 568, 739, 1098, 1117, 1279 |
| BstSLI | GKGCMC | 1 cut(s) | 1050 |
| BstUI | CGCG | 1 cut(s) | 820 |
| BstV1I | GCAGC | 1 cut(s) | 682 |
| BstV2I | GAAGAC | 2 cut(s) | 713, 888 |
| BstX2I | RGATCY | 2 cut(s) | 163, 346 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 86 |
| BstYI | RGATCY | 2 cut(s) | 163, 346 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1137 |
| BsuI | GTATCC | 1 cut(s) | 410 |
| BsuRI | GGCC | 5 cut(s) | 128, 292, 960, 964, 1207 |
| BtgI | CCRYGG | 1 cut(s) | 293 |
| BtrI | CACGTC | 1 cut(s) | 514 |
| BtsCI | GGATG | 3 cut(s) | 38, 728, 980 |
| Cac8I | GCNNGC | 6 cut(s) | 55, 408, 820, 860, 962, 1033 |
| CaiI | CAGNNNCTG | 2 cut(s) | 59, 627 |
| CciI | TCATGA | 3 cut(s) | 432, 643, 843 |
| CfoI | GCGC | 3 cut(s) | 102, 820, 822 |
| Cfr10I | RCCGGY | 1 cut(s) | 960 |
| Cfr13I | GGNCC | 2 cut(s) | 290, 922 |
| Csp6I | GTAC | 2 cut(s) | 225, 1064 |
| CviAII | CATG | 6 cut(s) | 364, 433, 521, 644, 686, 844 |
| CviQI | GTAC | 2 cut(s) | 225, 1064 |
| DdeI | CTNAG | 5 cut(s) | 49, 111, 353, 1137, 1265 |
| DpnI | GATC | 6 cut(s) | 91, 165, 234, 255, 348, 1175 |
| DpnII | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| DraI | TTTAAA | 1 cut(s) | 282 |
| EaeI | YGGCCR | 1 cut(s) | 126 |
| Eam1104I | CTCTTC | 4 cut(s) | 380, 542, 759, 1251 |
| EarI | CTCTTC | 4 cut(s) | 380, 542, 759, 1251 |
| Eco130I | CCWWGG | 3 cut(s) | 649, 918, 945 |
| Eco31I | GGTCTC | 1 cut(s) | 1109 |
| Eco47I | GGWCC | 1 cut(s) | 922 |
| Eco57I | CTGAAG | 2 cut(s) | 566, 1275 |
| Eco81I | CCTNAGG | 1 cut(s) | 1137 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1140 |
| EcoRI | GAATTC | 1 cut(s) | 1070 |
| EcoRII | CCWGG | 3 cut(s) | 568, 1098, 1279 |
| EcoT14I | CCWWGG | 3 cut(s) | 649, 918, 945 |
| EcoT22I | ATGCAT | 1 cut(s) | 982 |
| ErhI | CCWWGG | 3 cut(s) | 649, 918, 945 |
| FaeI | CATG | 6 cut(s) | 367, 436, 524, 647, 689, 847 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 153, 185, 1248, 1280 |
| FaqI | GGGAC | 2 cut(s) | 638, 908 |
| FatI | CATG | 6 cut(s) | 363, 432, 520, 643, 685, 843 |
| FauI | CCCGC | 1 cut(s) | 411 |
| FauNDI | CATATG | 1 cut(s) | 976 |
| FbaI | TGATCA | 1 cut(s) | 1173 |
| Fnu4HI | GCNGC | 1 cut(s) | 696 |
| FokI | GGATG | 3 cut(s) | 25, 715, 967 |
| FseI | GGCCGGCC | 1 cut(s) | 964 |
| Fsp4HI | GCNGC | 1 cut(s) | 696 |
| FspBI | CTAG | 4 cut(s) | 413, 1032, 1293, 1299 |
| GlaI | GCGC | 3 cut(s) | 101, 819, 821 |
| GluI | GCNGC | 1 cut(s) | 696 |
| GsuI | CTGGAG | 1 cut(s) | 591 |
| HaeII | RGCGCY | 1 cut(s) | 103 |
| HaeIII | GGCC | 5 cut(s) | 128, 292, 960, 964, 1207 |
| HapII | CCGG | 4 cut(s) | 314, 741, 961, 1118 |
| HhaI | GCGC | 3 cut(s) | 102, 820, 822 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 6 cut(s) | 367, 436, 524, 647, 689, 847 |
| Hin6I | GCGC | 3 cut(s) | 100, 818, 820 |
| HinP1I | GCGC | 3 cut(s) | 100, 818, 820 |
| HincII | GTYRAC | 1 cut(s) | 70 |
| HindII | GTYRAC | 1 cut(s) | 70 |
| HinfI | GANTC | 4 cut(s) | 367, 553, 938, 1160 |
| HpaII | CCGG | 4 cut(s) | 314, 741, 961, 1118 |
| HphI | GGTGA | 4 cut(s) | 37, 961, 963, 1031 |
| Hpy166II | GTNNAC | 2 cut(s) | 70, 511 |
| Hpy188I | TCNGA | 5 cut(s) | 61, 94, 258, 546, 726 |
| Hpy188III | TCNNGA | 5 cut(s) | 161, 218, 433, 644, 844 |
| Hpy8I | GTNNAC | 2 cut(s) | 70, 511 |
| Hpy99I | CGWCG | 2 cut(s) | 20, 518 |
| HpyAV | CCTTC | 4 cut(s) | 253, 1010, 1136, 1242 |
| HpyCH4III | ACNGT | 2 cut(s) | 150, 682 |
| HpyCH4IV | ACGT | 5 cut(s) | 5, 18, 262, 513, 773 |
| HpyCH4V | TGCA | 6 cut(s) | 343, 621, 685, 874, 980, 1168 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 32, 627, 859, 1275 |
| HpyF3I | CTNAG | 5 cut(s) | 49, 111, 353, 1137, 1265 |
| HpySE526I | ACGT | 5 cut(s) | 5, 18, 262, 513, 773 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 6 cut(s) | 367, 436, 524, 647, 689, 847 |
| HspAI | GCGC | 3 cut(s) | 100, 818, 820 |
| KroI | GCCGGC | 1 cut(s) | 960 |
| KroNI | GCCGGC | 1 cut(s) | 962 |
| Ksp22I | TGATCA | 1 cut(s) | 1173 |
| Kzo9I | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| LguI | GCTCTTC | 2 cut(s) | 380, 759 |
| LmnI | GCTCC | 4 cut(s) | 203, 316, 858, 1120 |
| Lsp1109I | GCAGC | 1 cut(s) | 682 |
| LweI | GCATC | 3 cut(s) | 195, 661, 989 |
| MaeI | CTAG | 4 cut(s) | 413, 1032, 1293, 1299 |
| MaeII | ACGT | 5 cut(s) | 5, 18, 262, 513, 773 |
| MaeIII | GTNAC | 2 cut(s) | 19, 263 |
| MalI | GATC | 6 cut(s) | 91, 165, 234, 255, 348, 1175 |
| MboI | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| MboII | GAAGA | 8 cut(s) | 367, 370, 559, 718, 746, 893, 1252, 1268 |
| MflI | RGATCY | 2 cut(s) | 163, 346 |
| MhlI | GDGCHC | 1 cut(s) | 1050 |
| MlsI | TGGCCA | 1 cut(s) | 128 |
| MluCI | AATT | 7 cut(s) | 501, 585, 734, 1055, 1070, 1083, 1131 |
| MluNI | TGGCCA | 1 cut(s) | 128 |
| MlyI | GAGTC | 2 cut(s) | 547, 947 |
| Mox20I | TGGCCA | 1 cut(s) | 128 |
| Mph1103I | ATGCAT | 1 cut(s) | 982 |
| MroNI | GCCGGC | 1 cut(s) | 960 |
| MscI | TGGCCA | 1 cut(s) | 128 |
| MseI | TTAA | 6 cut(s) | 210, 281, 608, 906, 1011, 1305 |
| MslI | CAYNNNNRTG | 3 cut(s) | 84, 362, 897 |
| Msp20I | TGGCCA | 1 cut(s) | 128 |
| MspCI | CTTAAG | 1 cut(s) | 607 |
| MspI | CCGG | 4 cut(s) | 314, 741, 961, 1118 |
| MspR9I | CCNGG | 5 cut(s) | 570, 741, 1100, 1119, 1281 |
| Mva1269I | GAATGC | 1 cut(s) | 357 |
| MvaI | CCWGG | 3 cut(s) | 570, 1100, 1281 |
| MvnI | CGCG | 1 cut(s) | 820 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 32, 627, 859, 1275 |
| NaeI | GCCGGC | 1 cut(s) | 962 |
| NciI | CCSGG | 2 cut(s) | 741, 1119 |
| NdeI | CATATG | 1 cut(s) | 976 |
| NdeII | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| NgoMIV | GCCGGC | 1 cut(s) | 960 |
| NheI | GCTAGC | 1 cut(s) | 1031 |
| NlaIII | CATG | 6 cut(s) | 367, 436, 524, 647, 689, 847 |
| NlaIV | GGNNCC | 3 cut(s) | 205, 924, 1047 |
| NmuCI | GTSAC | 1 cut(s) | 263 |
| NsiI | ATGCAT | 1 cut(s) | 982 |
| NspV | TTCGAA | 1 cut(s) | 704 |
| PagI | TCATGA | 3 cut(s) | 432, 643, 843 |
| PasI | CCCWGGG | 1 cut(s) | 1099 |
| PauI | GCGCGC | 1 cut(s) | 818 |
| PciSI | GCTCTTC | 2 cut(s) | 380, 759 |
| PctI | GAATGC | 1 cut(s) | 357 |
| PdiI | GCCGGC | 1 cut(s) | 962 |
| PfeI | GAWTC | 2 cut(s) | 367, 1160 |
| PfoI | TCCNGGA | 2 cut(s) | 568, 1117 |
| PkrI | GCNGC | 1 cut(s) | 697 |
| PleI | GAGTC | 2 cut(s) | 547, 946 |
| PpsI | GAGTC | 2 cut(s) | 547, 946 |
| Psp1406I | AACGTT | 1 cut(s) | 773 |
| Psp6I | CCWGG | 3 cut(s) | 568, 1098, 1279 |
| PspGI | CCWGG | 3 cut(s) | 568, 1098, 1279 |
| PspN4I | GGNNCC | 3 cut(s) | 205, 924, 1047 |
| PspPI | GGNCC | 2 cut(s) | 290, 922 |
| PstNI | CAGNNNCTG | 2 cut(s) | 59, 627 |
| PsuI | RGATCY | 2 cut(s) | 163, 346 |
| PteI | GCGCGC | 1 cut(s) | 818 |
| RigI | GGCCGGCC | 1 cut(s) | 964 |
| RsaI | GTAC | 2 cut(s) | 226, 1065 |
| RsaNI | GTAC | 2 cut(s) | 225, 1064 |
| RseI | CAYNNNNRTG | 3 cut(s) | 84, 362, 897 |
| SapI | GCTCTTC | 2 cut(s) | 380, 759 |
| SaqAI | TTAA | 6 cut(s) | 210, 281, 608, 906, 1011, 1305 |
| SatI | GCNGC | 1 cut(s) | 696 |
| Sau3AI | GATC | 6 cut(s) | 89, 163, 232, 253, 346, 1173 |
| Sau96I | GGNCC | 2 cut(s) | 290, 922 |
| SchI | GAGTC | 2 cut(s) | 547, 947 |
| ScrFI | CCNGG | 5 cut(s) | 570, 741, 1100, 1119, 1281 |
| SduI | GDGCHC | 1 cut(s) | 1050 |
| SfaNI | GCATC | 3 cut(s) | 195, 661, 989 |
| SfuI | TTCGAA | 1 cut(s) | 704 |
| SinI | GGWCC | 1 cut(s) | 922 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 84, 362, 897 |
| SmlI | CTYRAG | 3 cut(s) | 159, 607, 886 |
| SmoI | CTYRAG | 3 cut(s) | 159, 607, 886 |
| Sse9I | AATT | 7 cut(s) | 501, 585, 734, 1055, 1070, 1083, 1131 |
| SsiI | CCGC | 2 cut(s) | 404, 856 |
| SspMI | CTAG | 4 cut(s) | 413, 1032, 1293, 1299 |
| StyD4I | CCNGG | 5 cut(s) | 568, 739, 1098, 1117, 1279 |
| StyI | CCWWGG | 3 cut(s) | 649, 918, 945 |
| TaaI | ACNGT | 2 cut(s) | 150, 682 |
| TaiI | ACGT | 5 cut(s) | 8, 21, 265, 516, 776 |
| TaqI | TCGA | 3 cut(s) | 471, 704, 1217 |
| TaqII | GACCGA | 1 cut(s) | 282 |
| TasI | AATT | 7 cut(s) | 501, 585, 734, 1055, 1070, 1083, 1131 |
| TfiI | GAWTC | 2 cut(s) | 367, 1160 |
| Tru1I | TTAA | 6 cut(s) | 210, 281, 608, 906, 1011, 1305 |
| Tru9I | TTAA | 6 cut(s) | 210, 281, 608, 906, 1011, 1305 |
| TseFI | GTSAC | 1 cut(s) | 263 |
| TseI | GCWGC | 1 cut(s) | 695 |
| Tsp45I | GTSAC | 1 cut(s) | 263 |
| TspDTI | ATGAA | 7 cut(s) | 380, 449, 632, 702, 746, 860, 1029 |
| Vha464I | CTTAAG | 1 cut(s) | 607 |
| VpaK11BI | GGWCC | 1 cut(s) | 922 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1140 |
| XapI | RAATTY | 2 cut(s) | 1070, 1083 |
| XspI | CTAG | 4 cut(s) | 413, 1032, 1293, 1299 |
| ZraI | GACGTC | 1 cut(s) | 6 |
| Zsp2I | ATGCAT | 1 cut(s) | 982 |