Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 413 |
| Acc36I | ACCTGC | 1 cut(s) | 385 |
| Acc65I | GGTACC | 1 cut(s) | 392 |
| AccB1I | GGYRCC | 1 cut(s) | 392 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 154, 259 |
| AciI | CCGC | 2 cut(s) | 137, 640 |
| AclWI | GGATC | 1 cut(s) | 181 |
| AcsI | RAATTY | 4 cut(s) | 143, 270, 505, 677 |
| AdeI | CACNNNGTG | 1 cut(s) | 259 |
| AfaI | GTAC | 2 cut(s) | 394, 564 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 154, 247, 259, 383, 501, 821 |
| AgsI | TTSAA | 3 cut(s) | 11, 445, 579 |
| AjnI | CCWGG | 2 cut(s) | 147, 153 |
| AluBI | AGCT | 3 cut(s) | 166, 247, 455 |
| AluI | AGCT | 3 cut(s) | 166, 247, 455 |
| Alw26I | GTCTC | 1 cut(s) | 767 |
| AlwI | GGATC | 1 cut(s) | 181 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 154 |
| AoxI | GGCC | 4 cut(s) | 22, 108, 126, 249 |
| ApeKI | GCWGC | 3 cut(s) | 370, 707, 877 |
| ApoI | RAATTY | 4 cut(s) | 143, 270, 505, 677 |
| Asp718I | GGTACC | 1 cut(s) | 392 |
| AspLEI | GCGC | 2 cut(s) | 414, 540 |
| AspS9I | GGNCC | 2 cut(s) | 151, 250 |
| AsuC2I | CCSGG | 1 cut(s) | 191 |
| AsuHPI | GGTGA | 3 cut(s) | 205, 642, 654 |
| AvaII | GGWCC | 1 cut(s) | 151 |
| BanI | GGYRCC | 1 cut(s) | 392 |
| BbsI | GAAGAC | 1 cut(s) | 433 |
| BbvI | GCAGC | 3 cut(s) | 382, 694, 864 |
| BccI | CCATC | 3 cut(s) | 14, 329, 815 |
| BciT130I | CCWGG | 2 cut(s) | 149, 155 |
| BclI | TGATCA | 2 cut(s) | 3, 589 |
| BcnI | CCSGG | 1 cut(s) | 191 |
| BcoDI | GTCTC | 1 cut(s) | 767 |
| BfaI | CTAG | 5 cut(s) | 26, 98, 293, 611, 780 |
| BfuAI | ACCTGC | 1 cut(s) | 385 |
| BisI | GCNGC | 4 cut(s) | 371, 640, 708, 878 |
| BlsI | GCNGC | 4 cut(s) | 372, 641, 709, 879 |
| Bme1390I | CCNGG | 3 cut(s) | 149, 155, 191 |
| Bme18I | GGWCC | 1 cut(s) | 151 |
| BmgT120I | GGNCC | 2 cut(s) | 151, 250 |
| BmiI | GGNNCC | 2 cut(s) | 394, 638 |
| BmrFI | CCNGG | 3 cut(s) | 149, 155, 191 |
| BmsI | GCATC | 2 cut(s) | 611, 801 |
| BpiI | GAAGAC | 1 cut(s) | 433 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 628, 660 |
| Bpu10I | CCTNAGC | 1 cut(s) | 598 |
| BpuMI | CCSGG | 1 cut(s) | 191 |
| BsaJI | CCNNGG | 1 cut(s) | 816 |
| BsaWI | WCCGGW | 1 cut(s) | 361 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 816, 846 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 154, 247, 259, 383, 501, 821 |
| Bse118I | RCCGGY | 1 cut(s) | 571 |
| Bse1I | ACTGG | 3 cut(s) | 184, 394, 545 |
| Bse3DI | GCAATG | 1 cut(s) | 495 |
| BseBI | CCWGG | 2 cut(s) | 149, 155 |
| BseDI | CCNNGG | 1 cut(s) | 816 |
| BseGI | GGATG | 2 cut(s) | 340, 826 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 154, 247, 259, 383, 501, 821 |
| BseMI | GCAATG | 1 cut(s) | 495 |
| BseNI | ACTGG | 3 cut(s) | 184, 394, 545 |
| BseRI | GAGGAG | 2 cut(s) | 632, 840 |
| BseXI | GCAGC | 3 cut(s) | 382, 694, 864 |
| BseYI | CCCAGC | 1 cut(s) | 35 |
| BsgI | GTGCAG | 4 cut(s) | 78, 494, 693, 863 |
| BshFI | GGCC | 4 cut(s) | 24, 110, 128, 251 |
| BshNI | GGYRCC | 1 cut(s) | 392 |
| BsiSI | CCGG | 3 cut(s) | 190, 362, 572 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 154, 247, 259, 383, 501, 821 |
| BsmAI | GTCTC | 1 cut(s) | 767 |
| BsnI | GGCC | 4 cut(s) | 24, 110, 128, 251 |
| Bsp1407I | TGTACA | 1 cut(s) | 562 |
| Bsp143I | GATC | 4 cut(s) | 3, 75, 186, 589 |
| BspACI | CCGC | 2 cut(s) | 137, 640 |
| BspANI | GGCC | 4 cut(s) | 24, 110, 128, 251 |
| BspHI | TCATGA | 2 cut(s) | 205, 228 |
| BspLI | GGNNCC | 2 cut(s) | 394, 638 |
| BspMI | ACCTGC | 1 cut(s) | 385 |
| BspPI | GGATC | 1 cut(s) | 181 |
| BspQI | GCTCTTC | 1 cut(s) | 173 |
| BspT107I | GGYRCC | 1 cut(s) | 392 |
| BsrDI | GCAATG | 1 cut(s) | 495 |
| BsrFI | RCCGGY | 1 cut(s) | 571 |
| BsrGI | TGTACA | 1 cut(s) | 562 |
| BsrI | ACTGG | 3 cut(s) | 184, 394, 545 |
| BssAI | RCCGGY | 1 cut(s) | 571 |
| BssECI | CCNNGG | 1 cut(s) | 816 |
| BssMI | GATC | 4 cut(s) | 3, 75, 186, 589 |
| BssT1I | CCWWGG | 1 cut(s) | 816 |
| Bst2UI | CCWGG | 2 cut(s) | 149, 155 |
| Bst4CI | ACNGT | 2 cut(s) | 43, 854 |
| Bst6I | CTCTTC | 1 cut(s) | 173 |
| BstAUI | TGTACA | 1 cut(s) | 562 |
| BstC8I | GCNNGC | 3 cut(s) | 139, 249, 542 |
| BstDEI | CTNAG | 3 cut(s) | 216, 598, 666 |
| BstF5I | GGATG | 2 cut(s) | 340, 826 |
| BstHHI | GCGC | 2 cut(s) | 414, 540 |
| BstKTI | GATC | 4 cut(s) | 6, 78, 189, 592 |
| BstMAI | GTCTC | 1 cut(s) | 767 |
| BstMBI | GATC | 4 cut(s) | 3, 75, 186, 589 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 134, 707, 877 |
| BstNI | CCWGG | 2 cut(s) | 149, 155 |
| BstNSI | RCATGY | 1 cut(s) | 563 |
| BstSCI | CCNGG | 3 cut(s) | 147, 153, 189 |
| BstV1I | GCAGC | 3 cut(s) | 382, 694, 864 |
| BstV2I | GAAGAC | 1 cut(s) | 433 |
| BstX2I | RGATCY | 1 cut(s) | 186 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 335 |
| BstYI | RGATCY | 1 cut(s) | 186 |
| BsuRI | GGCC | 4 cut(s) | 24, 110, 128, 251 |
| BtsCI | GGATG | 2 cut(s) | 340, 826 |
| BtsI | GCAGTG | 2 cut(s) | 696, 866 |
| BtsIMutI | CAGTG | 6 cut(s) | 39, 585, 626, 696, 804, 866 |
| BveI | ACCTGC | 1 cut(s) | 385 |
| Cac8I | GCNNGC | 3 cut(s) | 139, 249, 542 |
| CaiI | CAGNNNCTG | 1 cut(s) | 154 |
| CciI | TCATGA | 2 cut(s) | 205, 228 |
| CfoI | GCGC | 2 cut(s) | 414, 540 |
| Cfr10I | RCCGGY | 1 cut(s) | 571 |
| Cfr13I | GGNCC | 2 cut(s) | 151, 250 |
| Csp6I | GTAC | 2 cut(s) | 393, 563 |
| CviAII | CATG | 4 cut(s) | 206, 229, 329, 560 |
| CviQI | GTAC | 2 cut(s) | 393, 563 |
| DdeI | CTNAG | 3 cut(s) | 216, 598, 666 |
| DpnI | GATC | 4 cut(s) | 5, 77, 188, 591 |
| DpnII | GATC | 4 cut(s) | 3, 75, 186, 589 |
| DraI | TTTAAA | 1 cut(s) | 466 |
| DraIII | CACNNNGTG | 1 cut(s) | 259 |
| Eam1104I | CTCTTC | 1 cut(s) | 173 |
| EarI | CTCTTC | 1 cut(s) | 173 |
| Eco130I | CCWWGG | 1 cut(s) | 816 |
| Eco32I | GATATC | 1 cut(s) | 211 |
| Eco47I | GGWCC | 1 cut(s) | 151 |
| EcoO109I | RGGNCCY | 1 cut(s) | 151 |
| EcoRII | CCWGG | 2 cut(s) | 147, 153 |
| EcoRV | GATATC | 1 cut(s) | 211 |
| EcoT14I | CCWWGG | 1 cut(s) | 816 |
| ErhI | CCWWGG | 1 cut(s) | 816 |
| FaeI | CATG | 4 cut(s) | 209, 232, 332, 563 |
| FatI | CATG | 4 cut(s) | 205, 228, 328, 559 |
| FauI | CCCGC | 1 cut(s) | 130 |
| FauNDI | CATATG | 2 cut(s) | 16, 311 |
| FbaI | TGATCA | 2 cut(s) | 3, 589 |
| Fnu4HI | GCNGC | 4 cut(s) | 371, 640, 708, 878 |
| FokI | GGATG | 2 cut(s) | 347, 833 |
| Fsp4HI | GCNGC | 4 cut(s) | 371, 640, 708, 878 |
| FspBI | CTAG | 5 cut(s) | 26, 98, 293, 611, 780 |
| FspI | TGCGCA | 1 cut(s) | 413 |
| GlaI | GCGC | 2 cut(s) | 413, 539 |
| GluI | GCNGC | 4 cut(s) | 371, 640, 708, 878 |
| GsaI | CCCAGC | 1 cut(s) | 39 |
| HaeIII | GGCC | 4 cut(s) | 24, 110, 128, 251 |
| HapII | CCGG | 3 cut(s) | 190, 362, 572 |
| HhaI | GCGC | 2 cut(s) | 414, 540 |
| Hin1II | CATG | 4 cut(s) | 209, 232, 332, 563 |
| Hin6I | GCGC | 2 cut(s) | 412, 538 |
| HinP1I | GCGC | 2 cut(s) | 412, 538 |
| HinfI | GANTC | 5 cut(s) | 31, 53, 478, 607, 775 |
| HpaII | CCGG | 3 cut(s) | 190, 362, 572 |
| HphI | GGTGA | 3 cut(s) | 205, 642, 654 |
| Hpy188I | TCNGA | 1 cut(s) | 504 |
| Hpy188III | TCNNGA | 3 cut(s) | 73, 206, 229 |
| HpyAV | CCTTC | 1 cut(s) | 657 |
| HpyCH4III | ACNGT | 2 cut(s) | 43, 854 |
| HpyCH4IV | ACGT | 2 cut(s) | 123, 341 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 134, 707, 877 |
| HpyF3I | CTNAG | 3 cut(s) | 216, 598, 666 |
| HpySE526I | ACGT | 2 cut(s) | 123, 341 |
| Hsp92II | CATG | 4 cut(s) | 209, 232, 332, 563 |
| HspAI | GCGC | 2 cut(s) | 412, 538 |
| KpnI | GGTACC | 1 cut(s) | 396 |
| Ksp22I | TGATCA | 2 cut(s) | 3, 589 |
| Kzo9I | GATC | 4 cut(s) | 3, 75, 186, 589 |
| LguI | GCTCTTC | 1 cut(s) | 173 |
| LmnI | GCTCC | 2 cut(s) | 452, 636 |
| Lsp1109I | GCAGC | 3 cut(s) | 382, 694, 864 |
| LweI | GCATC | 2 cut(s) | 611, 801 |
| MaeI | CTAG | 5 cut(s) | 26, 98, 293, 611, 780 |
| MaeII | ACGT | 2 cut(s) | 123, 341 |
| MaeIII | GTNAC | 2 cut(s) | 581, 848 |
| MalI | GATC | 4 cut(s) | 5, 77, 188, 591 |
| MboI | GATC | 4 cut(s) | 3, 75, 186, 589 |
| MboII | GAAGA | 8 cut(s) | 55, 80, 160, 271, 278, 433, 701, 871 |
| MflI | RGATCY | 1 cut(s) | 186 |
| MluCI | AATT | 9 cut(s) | 143, 270, 316, 402, 415, 427, 483, 505, 677 |
| MlyI | GAGTC | 4 cut(s) | 47, 472, 601, 769 |
| MmeI | TCCRAC | 2 cut(s) | 666, 836 |
| MnlI | CCTC | 7 cut(s) | 139, 325, 502, 610, 654, 796, 818 |
| MseI | TTAA | 4 cut(s) | 105, 426, 465, 674 |
| MslI | CAYNNNNRTG | 1 cut(s) | 333 |
| MspI | CCGG | 3 cut(s) | 190, 362, 572 |
| MspR9I | CCNGG | 3 cut(s) | 149, 155, 191 |
| MvaI | CCWGG | 2 cut(s) | 149, 155 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 134, 707, 877 |
| NciI | CCSGG | 1 cut(s) | 191 |
| NdeI | CATATG | 2 cut(s) | 16, 311 |
| NdeII | GATC | 4 cut(s) | 3, 75, 186, 589 |
| NlaIII | CATG | 4 cut(s) | 209, 232, 332, 563 |
| NlaIV | GGNNCC | 2 cut(s) | 394, 638 |
| NmuCI | GTSAC | 1 cut(s) | 581 |
| NsbI | TGCGCA | 1 cut(s) | 413 |
| NspI | RCATGY | 1 cut(s) | 563 |
| PagI | TCATGA | 2 cut(s) | 205, 228 |
| PciSI | GCTCTTC | 1 cut(s) | 173 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 154, 259 |
| PfoI | TCCNGGA | 1 cut(s) | 147 |
| PkrI | GCNGC | 4 cut(s) | 372, 641, 709, 879 |
| PleI | GAGTC | 4 cut(s) | 47, 472, 601, 769 |
| PpsI | GAGTC | 4 cut(s) | 47, 472, 601, 769 |
| PpuMI | RGGWCCY | 1 cut(s) | 151 |
| Psp5II | RGGWCCY | 1 cut(s) | 151 |
| Psp6I | CCWGG | 2 cut(s) | 147, 153 |
| PspFI | CCCAGC | 1 cut(s) | 35 |
| PspGI | CCWGG | 2 cut(s) | 147, 153 |
| PspN4I | GGNNCC | 2 cut(s) | 394, 638 |
| PspPI | GGNCC | 2 cut(s) | 151, 250 |
| PspPPI | RGGWCCY | 1 cut(s) | 151 |
| PstNI | CAGNNNCTG | 1 cut(s) | 154 |
| PsuI | RGATCY | 1 cut(s) | 186 |
| RsaI | GTAC | 2 cut(s) | 394, 564 |
| RsaNI | GTAC | 2 cut(s) | 393, 563 |
| RseI | CAYNNNNRTG | 1 cut(s) | 333 |
| SapI | GCTCTTC | 1 cut(s) | 173 |
| SaqAI | TTAA | 4 cut(s) | 105, 426, 465, 674 |
| SatI | GCNGC | 4 cut(s) | 371, 640, 708, 878 |
| Sau3AI | GATC | 4 cut(s) | 3, 75, 186, 589 |
| Sau96I | GGNCC | 2 cut(s) | 151, 250 |
| SchI | GAGTC | 4 cut(s) | 47, 472, 601, 769 |
| ScrFI | CCNGG | 3 cut(s) | 149, 155, 191 |
| SfaNI | GCATC | 2 cut(s) | 611, 801 |
| SinI | GGWCC | 1 cut(s) | 151 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 333 |
| Sse9I | AATT | 9 cut(s) | 143, 270, 316, 402, 415, 427, 483, 505, 677 |
| SsiI | CCGC | 2 cut(s) | 137, 640 |
| SspI | AATATT | 1 cut(s) | 85 |
| SspMI | CTAG | 5 cut(s) | 26, 98, 293, 611, 780 |
| StyD4I | CCNGG | 3 cut(s) | 147, 153, 189 |
| StyI | CCWWGG | 1 cut(s) | 816 |
| TaaI | ACNGT | 2 cut(s) | 43, 854 |
| TaiI | ACGT | 2 cut(s) | 126, 344 |
| TaqI | TCGA | 4 cut(s) | 78, 171, 400, 605 |
| TasI | AATT | 9 cut(s) | 143, 270, 316, 402, 415, 427, 483, 505, 677 |
| TatI | WGTACW | 1 cut(s) | 562 |
| TauI | GCSGC | 1 cut(s) | 642 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| Tru1I | TTAA | 4 cut(s) | 105, 426, 465, 674 |
| Tru9I | TTAA | 4 cut(s) | 105, 426, 465, 674 |
| TscAI | CASTG | 6 cut(s) | 46, 592, 633, 703, 804, 873 |
| TseFI | GTSAC | 1 cut(s) | 581 |
| TseI | GCWGC | 3 cut(s) | 370, 707, 877 |
| Tsp45I | GTSAC | 1 cut(s) | 581 |
| TspDTI | ATGAA | 5 cut(s) | 217, 245, 408, 449, 731 |
| TspGWI | ACGGA | 1 cut(s) | 649 |
| TspRI | CASTG | 6 cut(s) | 46, 592, 633, 703, 804, 873 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 154, 259 |
| VpaK11BI | GGWCC | 1 cut(s) | 151 |
| XapI | RAATTY | 4 cut(s) | 143, 270, 505, 677 |
| XceI | RCATGY | 1 cut(s) | 563 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 121 |
| XspI | CTAG | 5 cut(s) | 26, 98, 293, 611, 780 |