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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 368 |
| AccB1I | GGYRCC | 6 cut(s) | 103, 288, 476, 547, 1045, 1069 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 114 |
| AccII | CGCG | 2 cut(s) | 218, 242 |
| AciI | CCGC | 3 cut(s) | 98, 157, 227 |
| AclI | AACGTT | 1 cut(s) | 426 |
| AclWI | GGATC | 2 cut(s) | 316, 329 |
| AcsI | RAATTY | 2 cut(s) | 586, 1076 |
| AcuI | CTGAAG | 3 cut(s) | 560, 739, 906 |
| AcyI | GRCGYC | 1 cut(s) | 1046 |
| AdeI | CACNNNGTG | 1 cut(s) | 114 |
| AfaI | GTAC | 4 cut(s) | 257, 277, 702, 942 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 114, 152, 163, 236, 887 |
| AgsI | TTSAA | 3 cut(s) | 282, 304, 563 |
| AjnI | CCWGG | 2 cut(s) | 346, 966 |
| AluBI | AGCT | 9 cut(s) | 30, 212, 422, 460, 544, 753, 759, 874, 1103 |
| AluI | AGCT | 9 cut(s) | 30, 212, 422, 460, 544, 753, 759, 874, 1103 |
| Alw21I | GWGCWC | 1 cut(s) | 214 |
| AlwI | GGATC | 2 cut(s) | 316, 329 |
| AoxI | GGCC | 3 cut(s) | 154, 170, 344 |
| ApeKI | GCWGC | 3 cut(s) | 35, 203, 1096 |
| ApoI | RAATTY | 2 cut(s) | 586, 1076 |
| AseI | ATTAAT | 1 cut(s) | 1062 |
| AspLEI | GCGC | 5 cut(s) | 49, 220, 242, 369, 1048 |
| AspS9I | GGNCC | 4 cut(s) | 154, 166, 177, 268 |
| AsuC2I | CCSGG | 1 cut(s) | 286 |
| AsuHPI | GGTGA | 1 cut(s) | 779 |
| AvaII | GGWCC | 3 cut(s) | 166, 177, 268 |
| AxyI | CCTNAGG | 2 cut(s) | 162, 858 |
| BaeGI | GKGCMC | 2 cut(s) | 106, 291 |
| BamHI | GGATCC | 1 cut(s) | 321 |
| BanI | GGYRCC | 6 cut(s) | 103, 288, 476, 547, 1045, 1069 |
| BanII | GRGCYC | 2 cut(s) | 214, 917 |
| Bbv12I | GWGCWC | 1 cut(s) | 214 |
| BbvI | GCAGC | 3 cut(s) | 47, 190, 1108 |
| BccI | CCATC | 3 cut(s) | 288, 889, 1025 |
| BceAI | ACGGC | 2 cut(s) | 111, 214 |
| BciT130I | CCWGG | 2 cut(s) | 348, 968 |
| BcnI | CCSGG | 1 cut(s) | 286 |
| BfaI | CTAG | 3 cut(s) | 506, 944, 1114 |
| BfmI | CTRYAG | 2 cut(s) | 149, 918 |
| BfoI | RGCGCY | 2 cut(s) | 50, 1049 |
| BisI | GCNGC | 4 cut(s) | 36, 157, 204, 1097 |
| BlpI | GCTNAGC | 1 cut(s) | 666 |
| BlsI | GCNGC | 4 cut(s) | 37, 158, 205, 1098 |
| BmcAI | AGTACT | 2 cut(s) | 257, 942 |
| Bme1390I | CCNGG | 3 cut(s) | 286, 348, 968 |
| Bme18I | GGWCC | 3 cut(s) | 166, 177, 268 |
| BmgT120I | GGNCC | 4 cut(s) | 154, 166, 177, 268 |
| BmiI | GGNNCC | 8 cut(s) | 105, 290, 323, 478, 549, 834, 1047, 1071 |
| BmrFI | CCNGG | 3 cut(s) | 286, 348, 968 |
| BmsI | GCATC | 3 cut(s) | 378, 937, 1091 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 666 |
| BpuMI | CCSGG | 1 cut(s) | 286 |
| BsaHI | GRCGYC | 1 cut(s) | 1046 |
| BsaJI | CCNNGG | 3 cut(s) | 42, 173, 271 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 114, 152, 163, 236, 887 |
| Bse1I | ACTGG | 1 cut(s) | 221 |
| Bse21I | CCTNAGG | 2 cut(s) | 162, 858 |
| BseBI | CCWGG | 2 cut(s) | 348, 968 |
| BseDI | CCNNGG | 3 cut(s) | 42, 173, 271 |
| BseGI | GGATG | 3 cut(s) | 378, 457, 1036 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 114, 152, 163, 236, 887 |
| BseMII | CTCAG | 3 cut(s) | 125, 176, 849 |
| BseNI | ACTGG | 1 cut(s) | 221 |
| BseRI | GAGGAG | 2 cut(s) | 642, 875 |
| BseSI | GKGCMC | 2 cut(s) | 106, 291 |
| BseXI | GCAGC | 3 cut(s) | 47, 190, 1108 |
| BseYI | CCCAGC | 1 cut(s) | 431 |
| BsgI | GTGCAG | 1 cut(s) | 1115 |
| Bsh1236I | CGCG | 2 cut(s) | 218, 242 |
| Bsh1285I | CGRYCG | 1 cut(s) | 246 |
| BshFI | GGCC | 3 cut(s) | 156, 172, 346 |
| BshNI | GGYRCC | 6 cut(s) | 103, 288, 476, 547, 1045, 1069 |
| BsiEI | CGRYCG | 1 cut(s) | 246 |
| BsiHKAI | GWGCWC | 1 cut(s) | 214 |
| BsiSI | CCGG | 4 cut(s) | 169, 285, 1049, 1073 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 114, 152, 163, 236, 887 |
| BsmI | GAATGC | 1 cut(s) | 934 |
| BsnI | GGCC | 3 cut(s) | 156, 172, 346 |
| Bsp1286I | GDGCHC | 4 cut(s) | 106, 214, 291, 917 |
| Bsp143I | GATC | 3 cut(s) | 243, 321, 769 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 666 |
| Bsp19I | CCATGG | 1 cut(s) | 42 |
| BspACI | CCGC | 3 cut(s) | 98, 157, 227 |
| BspANI | GGCC | 3 cut(s) | 156, 172, 346 |
| BspCNI | CTCAG | 3 cut(s) | 124, 175, 850 |
| BspFNI | CGCG | 2 cut(s) | 218, 242 |
| BspLI | GGNNCC | 8 cut(s) | 105, 290, 323, 478, 549, 834, 1047, 1071 |
| BspMAI | CTGCAG | 1 cut(s) | 153 |
| BspPI | GGATC | 2 cut(s) | 316, 329 |
| BspQI | GCTCTTC | 1 cut(s) | 390 |
| BspT107I | GGYRCC | 6 cut(s) | 103, 288, 476, 547, 1045, 1069 |
| BsrI | ACTGG | 1 cut(s) | 221 |
| BssECI | CCNNGG | 3 cut(s) | 42, 173, 271 |
| BssMI | GATC | 3 cut(s) | 243, 321, 769 |
| BssNI | GRCGYC | 1 cut(s) | 1046 |
| BssT1I | CCWWGG | 3 cut(s) | 42, 173, 271 |
| Bst2UI | CCWGG | 2 cut(s) | 348, 968 |
| Bst4CI | ACNGT | 5 cut(s) | 336, 724, 955, 1069, 1093 |
| Bst6I | CTCTTC | 1 cut(s) | 390 |
| BstACI | GRCGYC | 1 cut(s) | 1046 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 32 |
| BstC8I | GCNNGC | 5 cut(s) | 28, 51, 546, 1037, 1101 |
| BstDEI | CTNAG | 5 cut(s) | 6, 111, 162, 666, 858 |
| BstDSI | CCRYGG | 1 cut(s) | 42 |
| BstF5I | GGATG | 3 cut(s) | 378, 457, 1036 |
| BstFNI | CGCG | 2 cut(s) | 218, 242 |
| BstH2I | RGCGCY | 2 cut(s) | 50, 1049 |
| BstHHI | GCGC | 5 cut(s) | 49, 220, 242, 369, 1048 |
| BstKTI | GATC | 3 cut(s) | 246, 324, 772 |
| BstMBI | GATC | 3 cut(s) | 243, 321, 769 |
| BstMCI | CGRYCG | 1 cut(s) | 246 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 32, 44, 209, 226, 428, 1045 |
| BstNI | CCWGG | 2 cut(s) | 348, 968 |
| BstNSI | RCATGY | 1 cut(s) | 1039 |
| BstSCI | CCNGG | 3 cut(s) | 284, 346, 966 |
| BstSFI | CTRYAG | 2 cut(s) | 149, 918 |
| BstSLI | GKGCMC | 2 cut(s) | 106, 291 |
| BstUI | CGCG | 2 cut(s) | 218, 242 |
| BstV1I | GCAGC | 3 cut(s) | 47, 190, 1108 |
| BstX2I | RGATCY | 1 cut(s) | 321 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 632 |
| BstYI | RGATCY | 1 cut(s) | 321 |
| Bsu36I | CCTNAGG | 2 cut(s) | 162, 858 |
| BsuRI | GGCC | 3 cut(s) | 156, 172, 346 |
| BtgI | CCRYGG | 1 cut(s) | 42 |
| BtsCI | GGATG | 3 cut(s) | 378, 457, 1036 |
| BtsI | GCAGTG | 1 cut(s) | 1017 |
| BtsIMutI | CAGTG | 3 cut(s) | 119, 1017, 1098 |
| Cac8I | GCNNGC | 5 cut(s) | 28, 51, 546, 1037, 1101 |
| CfoI | GCGC | 5 cut(s) | 49, 220, 242, 369, 1048 |
| Cfr13I | GGNCC | 4 cut(s) | 154, 166, 177, 268 |
| CseI | GACGC | 2 cut(s) | 27, 194 |
| Csp6I | GTAC | 4 cut(s) | 256, 276, 701, 941 |
| CviAII | CATG | 7 cut(s) | 43, 342, 500, 626, 643, 746, 1036 |
| CviQI | GTAC | 4 cut(s) | 256, 276, 701, 941 |
| DdeI | CTNAG | 5 cut(s) | 6, 111, 162, 666, 858 |
| DinI | GGCGCC | 1 cut(s) | 1047 |
| DpnI | GATC | 3 cut(s) | 245, 323, 771 |
| DpnII | GATC | 3 cut(s) | 243, 321, 769 |
| DraI | TTTAAA | 1 cut(s) | 763 |
| DraIII | CACNNNGTG | 1 cut(s) | 114 |
| Eam1104I | CTCTTC | 1 cut(s) | 390 |
| EarI | CTCTTC | 1 cut(s) | 390 |
| EciI | GGCGGA | 1 cut(s) | 216 |
| Ecl136II | GAGCTC | 1 cut(s) | 212 |
| Eco130I | CCWWGG | 3 cut(s) | 42, 173, 271 |
| Eco24I | GRGCYC | 2 cut(s) | 214, 917 |
| Eco32I | GATATC | 1 cut(s) | 64 |
| Eco47I | GGWCC | 3 cut(s) | 166, 177, 268 |
| Eco53kI | GAGCTC | 1 cut(s) | 212 |
| Eco57I | CTGAAG | 3 cut(s) | 560, 739, 906 |
| Eco81I | CCTNAGG | 2 cut(s) | 162, 858 |
| EcoICRI | GAGCTC | 1 cut(s) | 212 |
| EcoO109I | RGGNCCY | 1 cut(s) | 268 |
| EcoRII | CCWGG | 2 cut(s) | 346, 966 |
| EcoRV | GATATC | 1 cut(s) | 64 |
| EcoT14I | CCWWGG | 3 cut(s) | 42, 173, 271 |
| EcoT22I | ATGCAT | 1 cut(s) | 644 |
| EcoT38I | GRGCYC | 2 cut(s) | 214, 917 |
| EgeI | GGCGCC | 1 cut(s) | 1047 |
| EheI | GGCGCC | 1 cut(s) | 1047 |
| ErhI | CCWWGG | 3 cut(s) | 42, 173, 271 |
| FaeI | CATG | 7 cut(s) | 46, 345, 503, 629, 646, 749, 1039 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 51, 83 |
| FatI | CATG | 7 cut(s) | 42, 341, 499, 625, 642, 745, 1035 |
| FauI | CCCGC | 1 cut(s) | 91 |
| Fnu4HI | GCNGC | 4 cut(s) | 36, 157, 204, 1097 |
| FokI | GGATG | 3 cut(s) | 365, 464, 1043 |
| FriOI | GRGCYC | 2 cut(s) | 214, 917 |
| Fsp4HI | GCNGC | 4 cut(s) | 36, 157, 204, 1097 |
| FspAI | RTGCGCAY | 1 cut(s) | 368 |
| FspBI | CTAG | 3 cut(s) | 506, 944, 1114 |
| FspI | TGCGCA | 1 cut(s) | 368 |
| GlaI | GCGC | 5 cut(s) | 48, 219, 241, 368, 1047 |
| GluI | GCNGC | 4 cut(s) | 36, 157, 204, 1097 |
| GsaI | CCCAGC | 1 cut(s) | 435 |
| HaeII | RGCGCY | 2 cut(s) | 50, 1049 |
| HaeIII | GGCC | 3 cut(s) | 156, 172, 346 |
| HapII | CCGG | 4 cut(s) | 169, 285, 1049, 1073 |
| HgaI | GACGC | 2 cut(s) | 27, 194 |
| HhaI | GCGC | 5 cut(s) | 49, 220, 242, 369, 1048 |
| Hin1I | GRCGYC | 1 cut(s) | 1046 |
| Hin1II | CATG | 7 cut(s) | 46, 345, 503, 629, 646, 749, 1039 |
| Hin6I | GCGC | 5 cut(s) | 47, 218, 240, 367, 1046 |
| HinP1I | GCGC | 5 cut(s) | 47, 218, 240, 367, 1046 |
| HincII | GTYRAC | 1 cut(s) | 339 |
| HindII | GTYRAC | 1 cut(s) | 339 |
| HindIII | AAGCTT | 2 cut(s) | 28, 458 |
| HinfI | GANTC | 1 cut(s) | 327 |
| HpaII | CCGG | 4 cut(s) | 169, 285, 1049, 1073 |
| HphI | GGTGA | 1 cut(s) | 779 |
| Hpy166II | GTNNAC | 3 cut(s) | 339, 821, 964 |
| Hpy188I | TCNGA | 8 cut(s) | 7, 184, 248, 326, 540, 805, 837, 886 |
| Hpy188III | TCNNGA | 2 cut(s) | 304, 718 |
| Hpy8I | GTNNAC | 3 cut(s) | 339, 821, 964 |
| Hpy99I | CGWCG | 1 cut(s) | 97 |
| HpyAV | CCTTC | 4 cut(s) | 666, 802, 881, 1103 |
| HpyCH4III | ACNGT | 5 cut(s) | 336, 724, 955, 1069, 1093 |
| HpyCH4IV | ACGT | 1 cut(s) | 426 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 32, 44, 209, 226, 428, 1045 |
| HpyF3I | CTNAG | 5 cut(s) | 6, 111, 162, 666, 858 |
| HpySE526I | ACGT | 1 cut(s) | 426 |
| Hsp92I | GRCGYC | 1 cut(s) | 1046 |
| Hsp92II | CATG | 7 cut(s) | 46, 345, 503, 629, 646, 749, 1039 |
| HspAI | GCGC | 5 cut(s) | 47, 218, 240, 367, 1046 |
| KasI | GGCGCC | 1 cut(s) | 1045 |
| Kzo9I | GATC | 3 cut(s) | 243, 321, 769 |
| LguI | GCTCTTC | 1 cut(s) | 390 |
| LmnI | GCTCC | 1 cut(s) | 750 |
| Lsp1109I | GCAGC | 3 cut(s) | 47, 190, 1108 |
| LweI | GCATC | 3 cut(s) | 378, 937, 1091 |
| MaeI | CTAG | 3 cut(s) | 506, 944, 1114 |
| MaeII | ACGT | 1 cut(s) | 426 |
| MaeIII | GTNAC | 2 cut(s) | 19, 197 |
| MalI | GATC | 3 cut(s) | 245, 323, 771 |
| MboI | GATC | 3 cut(s) | 243, 321, 769 |
| MboII | GAAGA | 3 cut(s) | 71, 407, 575 |
| MfeI | CAATTG | 1 cut(s) | 84 |
| MflI | RGATCY | 1 cut(s) | 321 |
| MhlI | GDGCHC | 4 cut(s) | 106, 214, 291, 917 |
| MluCI | AATT | 8 cut(s) | 84, 558, 586, 709, 975, 996, 1059, 1076 |
| Mly113I | GGCGCC | 1 cut(s) | 1046 |
| MmeI | TCCRAC | 2 cut(s) | 372, 399 |
| MnlI | CCTC | 9 cut(s) | 156, 171, 190, 259, 393, 484, 663, 853, 856 |
| Mph1103I | ATGCAT | 1 cut(s) | 644 |
| MseI | TTAA | 3 cut(s) | 465, 762, 1062 |
| MslI | CAYNNNNRTG | 2 cut(s) | 630, 1040 |
| MspI | CCGG | 4 cut(s) | 169, 285, 1049, 1073 |
| MspR9I | CCNGG | 3 cut(s) | 286, 348, 968 |
| MunI | CAATTG | 1 cut(s) | 84 |
| Mva1269I | GAATGC | 1 cut(s) | 934 |
| MvaI | CCWGG | 2 cut(s) | 348, 968 |
| MvnI | CGCG | 2 cut(s) | 218, 242 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 32, 44, 209, 226, 428, 1045 |
| NarI | GGCGCC | 1 cut(s) | 1046 |
| NciI | CCSGG | 1 cut(s) | 286 |
| NcoI | CCATGG | 1 cut(s) | 42 |
| NdeII | GATC | 3 cut(s) | 243, 321, 769 |
| NlaIII | CATG | 7 cut(s) | 46, 345, 503, 629, 646, 749, 1039 |
| NlaIV | GGNNCC | 8 cut(s) | 105, 290, 323, 478, 549, 834, 1047, 1071 |
| NsbI | TGCGCA | 1 cut(s) | 368 |
| NsiI | ATGCAT | 1 cut(s) | 644 |
| NspI | RCATGY | 1 cut(s) | 1039 |
| PaeI | GCATGC | 1 cut(s) | 1039 |
| PciSI | GCTCTTC | 1 cut(s) | 390 |
| PctI | GAATGC | 1 cut(s) | 934 |
| PfeI | GAWTC | 1 cut(s) | 327 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 114 |
| PkrI | GCNGC | 4 cut(s) | 37, 158, 205, 1098 |
| Ple19I | CGATCG | 1 cut(s) | 246 |
| PluTI | GGCGCC | 1 cut(s) | 1049 |
| PpuMI | RGGWCCY | 1 cut(s) | 268 |
| PshBI | ATTAAT | 1 cut(s) | 1062 |
| Psp124BI | GAGCTC | 1 cut(s) | 214 |
| Psp1406I | AACGTT | 1 cut(s) | 426 |
| Psp5II | RGGWCCY | 1 cut(s) | 268 |
| Psp6I | CCWGG | 2 cut(s) | 346, 966 |
| PspFI | CCCAGC | 1 cut(s) | 431 |
| PspGI | CCWGG | 2 cut(s) | 346, 966 |
| PspN4I | GGNNCC | 8 cut(s) | 105, 290, 323, 478, 549, 834, 1047, 1071 |
| PspPI | GGNCC | 4 cut(s) | 154, 166, 177, 268 |
| PspPPI | RGGWCCY | 1 cut(s) | 268 |
| PstI | CTGCAG | 1 cut(s) | 153 |
| PsuI | RGATCY | 1 cut(s) | 321 |
| PvuI | CGATCG | 1 cut(s) | 246 |
| RsaI | GTAC | 4 cut(s) | 257, 277, 702, 942 |
| RsaNI | GTAC | 4 cut(s) | 256, 276, 701, 941 |
| RseI | CAYNNNNRTG | 2 cut(s) | 630, 1040 |
| SacI | GAGCTC | 1 cut(s) | 214 |
| SapI | GCTCTTC | 1 cut(s) | 390 |
| SaqAI | TTAA | 3 cut(s) | 465, 762, 1062 |
| SatI | GCNGC | 4 cut(s) | 36, 157, 204, 1097 |
| Sau3AI | GATC | 3 cut(s) | 243, 321, 769 |
| Sau96I | GGNCC | 4 cut(s) | 154, 166, 177, 268 |
| ScaI | AGTACT | 2 cut(s) | 257, 942 |
| ScrFI | CCNGG | 3 cut(s) | 286, 348, 968 |
| SduI | GDGCHC | 4 cut(s) | 106, 214, 291, 917 |
| SfaNI | GCATC | 3 cut(s) | 378, 937, 1091 |
| SfcI | CTRYAG | 2 cut(s) | 149, 918 |
| SfoI | GGCGCC | 1 cut(s) | 1047 |
| SinI | GGWCC | 3 cut(s) | 166, 177, 268 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 630, 1040 |
| SphI | GCATGC | 1 cut(s) | 1039 |
| Sse9I | AATT | 8 cut(s) | 84, 558, 586, 709, 975, 996, 1059, 1076 |
| SsiI | CCGC | 3 cut(s) | 98, 157, 227 |
| SspDI | GGCGCC | 1 cut(s) | 1045 |
| SspI | AATATT | 1 cut(s) | 850 |
| SspMI | CTAG | 3 cut(s) | 506, 944, 1114 |
| SstI | GAGCTC | 1 cut(s) | 214 |
| StyD4I | CCNGG | 3 cut(s) | 284, 346, 966 |
| StyI | CCWWGG | 3 cut(s) | 42, 173, 271 |
| TaaI | ACNGT | 5 cut(s) | 336, 724, 955, 1069, 1093 |
| TaiI | ACGT | 1 cut(s) | 429 |
| TaqI | TCGA | 1 cut(s) | 659 |
| TasI | AATT | 8 cut(s) | 84, 558, 586, 709, 975, 996, 1059, 1076 |
| TatI | WGTACW | 3 cut(s) | 255, 700, 940 |
| TauI | GCSGC | 1 cut(s) | 159 |
| TfiI | GAWTC | 1 cut(s) | 327 |
| Tru1I | TTAA | 3 cut(s) | 465, 762, 1062 |
| Tru9I | TTAA | 3 cut(s) | 465, 762, 1062 |
| TscAI | CASTG | 3 cut(s) | 119, 1024, 1098 |
| TseI | GCWGC | 3 cut(s) | 35, 203, 1096 |
| TspDTI | ATGAA | 3 cut(s) | 30, 319, 488 |
| TspRI | CASTG | 3 cut(s) | 119, 1024, 1098 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 114 |
| VpaK11BI | GGWCC | 3 cut(s) | 166, 177, 268 |
| VspI | ATTAAT | 1 cut(s) | 1062 |
| XapI | RAATTY | 2 cut(s) | 586, 1076 |
| XceI | RCATGY | 1 cut(s) | 1039 |
| XspI | CTAG | 3 cut(s) | 506, 944, 1114 |
| ZrmI | AGTACT | 2 cut(s) | 257, 942 |
| Zsp2I | ATGCAT | 1 cut(s) | 644 |