FvH4_3g28251

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000182
Physical Location & Seq
Forward (+)
21233082 .. 21235523
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28251.t1

Sequence Viewer

Length: 2217 bp
ATGTTTACGAAAGTTAAAAGTTTCGAGGTTTACATGACACACGCCTGCTTCTCGAGCCTCTGGGCTCTGATAGGGTTTTTGTTCCTGTTCTTTGCTCTTGATTGGGGCTATTCTCGGGGGCCTCCTTGGCCTGATCCACCTCCCTTCTCCTCCTTGGGAATTAACCTTTCAGATCATTATCATGTCGTGACCCAAAAGCCCATAAAACCTTTTCGTTCCTTCACGTTTTGTGGTTTTGGAGATTGGGTTGCATATCACAATCAAATTTCCTGCAAGCTTAATCTGATTGGGAAGAGCGGCGGACGTCATTCGCAGCAGCAATTTGATGATTATTGCTTTCAATTTGTGATTTGGGAGAGTAATCAGATCAGAAGTAAGATTCATATCTCCTTGCGCAGAGATGGTAATGATAGCTCTATCCCTTCTTTTTTCCTCGATTCGGGAGCACAGGAATTTGTTTCGTTTCTCCCAAGTGGGGAAATTGCGACCACTATTTATACGTGCTTAGATGTTCGTTCTAAGCATCATCTTCTCTTTCTCTGGCTTATTCTAGTTCCCCAATTCCCCTCAAACATGGCCTCTCCTAACTCTCAAGCTTTCTCTTGTGCCCCTGGCCAATCCTTTAGTGTTTCTCGTAACCTTTTTCTTCGCCCAAGGGCAAATGAGTCTCATATCTCTGTCAATGTGGGTGATACCCGAATTACTGTTGATCAGAGAGACACTCATGTCACCAATAAGAAGCCGTCATCTTCCTATGTGGACCACTACACTCGATTCTGTCTTGTGGGGAGAATTTTTGGGAAGAAGCTTGATGCCGACACAGTGTTAGAGAAGGCGGCATCGATGTGGAATGATGTGCAGGGTACTCTTACACTCAACAAGCTGGCAAACAACTGGTTTCACTTGGAGTTTTCTCTAGAAGAAGATATCTCGTATGTGCTTGATGAGAGACCATGGTTTATCAAAGGGAGGATCTTCCATGTCCAGCGGTGGATCCCAGGTTTCAATCCCCTCTACTATCGTATTCAATCTCTTATTGCCTGGGTGAGATTGCATAACGTCCCAATGCACTATTGGGACCCTGAGTGTCTTGAAAGTGTTGTTATTCTCATTGGTAAATTTATTAAACTTGATAAGGCAACTGTTATGGGTTCCCAATGCTTGTTTGCTAGAGTCTGTGTGGAGCTTGACCTAAGCATTCCACTTAAAAGGTCCTTAGTTCTCCATGTTGAGGATGAGATGGAGGAGGCAGTCCACACTTTTGTCTCTTATGAAGGCTTGTTTACTGTTTGCTTTCAATGTGGCAATCACCGCCACAAAATCAATACATGCCCTATGCATGTAAGAGATGAAGGCTTCCTGTTAGTGGATAGAAACTCTGATGATGAAGTAGAACCTGCTGAGAAAGAGATTGGTCCAGAGATTCAAACTCTTATTTCTGAGCAAGTAATGGTGGTTTTCCCACTCCCTAATGCTGGAGCAGGTCGTGGACGTGCTCCGGCTAACTCCCAGGCAAAGGCTGAGGGCTTTAGTGACCTGAATAACAACTCTCCTAAATGGAATCAAGTTCCAGTCAAGAAGAGGTCTGGAGCCAAAGGCTACACTAGTGGAACGGGTATAGCTCCTACTGGGGTAAGGTCTATTAGGCCTAATTCGGGTAAGATCAAAGAGGGAGTCTCTTTTAAGGATGCAGCTAGAGGCGGTATAACAATTGGGCAGCGTAATGAGAATGTTGCTGGAGCCGGCCGTTTTGACCCTGAAACTCACTCTCAAGATTTGGTGAGTGGCTTTTCTGTGTTGGGAGAGCCAAGTTCTGTCCATTCTTTTTCTTCTTTAACTGATATTGTTCATCTTATGCATTCTTCCTGTCTGAATAATCTCTTCCCCCTTCCTATGGACCCAAATTCTATCTATTCTCCTTCCTCCACGGTTGACTTTTTTAATCATTTTTATGGACTTATTCATCACTCTCAAGAGAACTTTGAGGATGTCTATGAGGAGGACCAGATGATAAATAACAATGTTATGGATGGTGATTATGATGCTAATGGTATGTTTGGTGTTGTGAATGAGAATGATGAAATGGATGTGAGTGCTAGGTTTCGCAGCTCATTAAAGAGAACCAGAGAGATGATCGAAGATGAAGTGGATTCTCATCAGGCTAGGACCATGGAGGTTGGCCCCTCCAAGATGACAAACTTCTCTACTCCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

739

Amino Acids

83.38

Weight (kDa)

6.06

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 258 - 337 8.1e-13 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000182)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21471 FvH4_1g27953 FvH4_1g28801 FvH4_2g14522 FvH4_3g04280 FvH4_3g28251 FvH4_4g07491 FvH4_4g30262
malus_domestica MD10G1046400.v1.1 MD10G1126900.v1.1 MD11G1236100.v1.1
prunus_persica Prupe.1G148300_v2.0.a1 Prupe.2G036800_v2.0.a1 Prupe.8G054600_v2.0.a1 Prupe.8G054700_v2.0.a1
pyrus_communis pycom05g05520 pycom08g11160 pycom13g29480
rosa_chinensis RchiOBHm_Chr7g0182921 RchiOBHm_Chr7g0230111
rosa_laevigata RLG00000000536 RLG00000001385 RLG00000001492 RLG00000002640 RLG00000003082 RLG00000005042 RLG00000007254 RLG00000007773 RLG00000008594 RLG00000009508 RLG00000010355 RLG00000013369 RLG00000014370 RLG00000014371 RLG00000018745 RLG00000020268 RLG00000020832 RLG00000022569 RLG00000028073 RLG00000028388 RLG00000028677 RLG00000035645
rosa_multiflora Rmu_co8277499.1_g000001 Rmu_co8500999.1_g000001 Rmu_sc0000014.1_g000025 Rmu_sc0000103.1_g000003 Rmu_sc0000168.1_g000019 Rmu_sc0000283.1_g000006 Rmu_sc0000693.1_g000080 Rmu_sc0000997.1_g000005 Rmu_sc0001228.1_g000005 Rmu_sc0001231.1_g000023 Rmu_sc0002833.1_g000037 Rmu_sc0002869.1_g000015 Rmu_sc0003545.1_g000003 Rmu_sc0003693.1_g000028 Rmu_sc0004000.1_g000022 Rmu_sc0004454.1_g000007 Rmu_sc0004976.1_g000036 Rmu_sc0005014.1_g000013 Rmu_sc0005046.1_g000013 Rmu_sc0005063.1_g000009 Rmu_sc0005652.1_g000002 Rmu_sc0005697.1_g000008 Rmu_sc0006989.1_g000014 Rmu_sc0008957.1_g000003 Rmu_sc0011659.1_g000001 Rmu_sc0027477.1_g000001 Rmu_ssc0000387.1_g000021
rosa_roxburghii Rroxscaffold_1G00020860 Rroxscaffold_1G00035780 Rroxscaffold_1G00051370 Rroxscaffold_2G00101730 Rroxscaffold_3G00230130 Rroxscaffold_4G00280070 Rroxscaffold_4G00281160 Rroxscaffold_4G00318780 Rroxscaffold_5G00341570 Rroxscaffold_5G00341580 Rroxscaffold_7G00191230 Rroxscaffold_7G00195880 Rroxscaffold_7G00195890 Rroxscaffold_7G00209080 Rroxscaffold_7G00209090
rosa_rugosa Rorug02G0170400 Rorug03G0234500 Rorug04G0045200 Rorug07G0191000
rosa_samantha Rh1AG012200 Rh1AG012300 Rh1AG021700 Rh1AG033800 Rh1AG033900 Rh1AG048100 Rh1AG087000 Rh1AG168600 Rh1CG004600 Rh2BG192700 Rh2BG200800 Rh2BG310200 Rh2BG356900 Rh2BG377900 Rh2BG378000 Rh2CG356300 Rh2DG394800 Rh3AG199000 Rh3AG258700 Rh3AG258800 Rh3AG324400 Rh3BG202300 Rh3BG202400 Rh3BG228800 Rh3BG318800 Rh3BG360400 Rh3BG368300 Rh3BG373700 Rh4AG071400 Rh4AG153700 Rh4AG224300 Rh4AG224400 Rh4CG044000 Rh4CG077200 Rh4CG077300 Rh4CG238700 Rh4CG238800 Rh4CG238900 Rh5BG367100 Rh5BG379500 Rh5BG379600 Rh5BG396700 Rh5BG412900 Rh5DG427200 Rh6AG015200 Rh6AG015300 Rh6AG122800 Rh6AG175600 Rh6AG187700 Rh6AG187800 Rh6AG260800 Rh6AG355000 Rh6BG074900 Rh6BG102300 Rh7AG127400 Rh7AG157500 Rh7AG157600 Rh7AG212200 Rh7AG331400 Rh7AG412000 Rh7CG055400 Rh7CG131400 Rh7CG131500 Rh7CG224300 Rh7CG267100 Rh7CG348800
rosa_wichuraiana Rw0G009820 Rw1G000520 Rw1G008820 Rw4G014110 Rw4G019370 Rw4G022440 Rw6G010550 Rw7G001520 Rw7G018400 Rw7G025210 Rw7G027910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 725
AatII GACGTC 1 cut(s) 307
Acc16I TGCGCA 1 cut(s) 395
Acc36I ACCTGC 2 cut(s) 1405, 1472
AccBSI CCGCTC 1 cut(s) 297
AciI CCGC 6 cut(s) 297, 300, 836, 988, 1312, 1701
AclWI GGATC 4 cut(s) 128, 980, 988, 1001
AcoI YGGCCR 2 cut(s) 613, 1744
AcsI RAATTY 5 cut(s) 264, 452, 792, 1118, 1903
AcyI GRCGYC 1 cut(s) 304
AfaI GTAC 1 cut(s) 865
AfiI CCNNNNNNNGG 8 cut(s) 439, 475, 1231, 1366, 1475, 1516, 1655, 1894
AgsI TTSAA 6 cut(s) 341, 1006, 1028, 1094, 1298, 1427
AhlI ACTAGT 1 cut(s) 1604
AjiI CACGTC 1 cut(s) 1493
AjnI CCWGG 4 cut(s) 610, 997, 1040, 1509
AluBI AGCT 9 cut(s) 277, 414, 596, 808, 883, 1186, 1622, 1694, 2109
AluI AGCT 9 cut(s) 277, 414, 596, 808, 883, 1186, 1622, 1694, 2109
Alw21I GWGCWC 2 cut(s) 448, 1498
Alw26I GTCTC 5 cut(s) 672, 711, 943, 1270, 1681
AlwI GGATC 4 cut(s) 128, 980, 988, 1001
Ama87I CYCGRG 2 cut(s) 52, 114
AoxI GGCC 7 cut(s) 119, 128, 576, 613, 1646, 1744, 2179
ApeKI GCWGC 5 cut(s) 313, 316, 1691, 1717, 2106
ApoI RAATTY 5 cut(s) 264, 452, 792, 1118, 1903
ArsI GACNNNNNNTTYG 2 cut(s) 1659, 1691
AspLEI GCGC 1 cut(s) 396
AspS9I GGNCC 9 cut(s) 119, 760, 1078, 1212, 1415, 1897, 2002, 2166, 2180
AsuHPI GGTGA 6 cut(s) 701, 721, 1057, 1301, 1792, 2045
AvaI CYCGRG 2 cut(s) 52, 114
AvaII GGWCC 7 cut(s) 760, 1078, 1212, 1415, 1897, 2002, 2166
BaeGI GKGCMC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 615
BamHI GGATCC 1 cut(s) 993
BanII GRGCYC 1 cut(s) 67
Bbv12I GWGCWC 2 cut(s) 448, 1498
BbvCI CCTCAGC 1 cut(s) 1521
BbvI GCAGC 5 cut(s) 325, 328, 1703, 1729, 2118
BccI CCATC 3 cut(s) 395, 1234, 2024
BceAI ACGGC 2 cut(s) 727, 1731
BciT130I CCWGG 4 cut(s) 612, 999, 1042, 1511
BclI TGATCA 1 cut(s) 709
BcoDI GTCTC 5 cut(s) 672, 711, 943, 1270, 1681
BcuI ACTAGT 1 cut(s) 1604
BfaI CTAG 7 cut(s) 551, 917, 1170, 1605, 1695, 2097, 2163
BfuAI ACCTGC 2 cut(s) 1405, 1472
BglI GCCNNNNNGGC 1 cut(s) 127
BisI GCNGC 7 cut(s) 298, 314, 317, 837, 1692, 1718, 2107
BlsI GCNGC 7 cut(s) 299, 315, 318, 838, 1693, 1719, 2108
Bme1390I CCNGG 4 cut(s) 612, 999, 1042, 1511
Bme18I GGWCC 7 cut(s) 760, 1078, 1212, 1415, 1897, 2002, 2166
BmeT110I CYCGRG 2 cut(s) 52, 114
BmgBI CACGTC 1 cut(s) 1493
BmgT120I GGNCC 9 cut(s) 119, 760, 1078, 1212, 1415, 1897, 2002, 2166, 2180
BmiI GGNNCC 9 cut(s) 120, 995, 1079, 1080, 1153, 1591, 1741, 1899, 2182
BmrFI CCNGG 4 cut(s) 612, 999, 1042, 1511
BmrI ACTGGG 1 cut(s) 1638
BmsI GCATC 5 cut(s) 532, 802, 848, 1678, 2032
BmuI ACTGGG 1 cut(s) 1638
BplI GAGNNNNNCTC 4 cut(s) 706, 738, 1661, 1693
BpmI CTGGAG 3 cut(s) 1497, 1608, 1758
Bpu10I CCTNAGC 2 cut(s) 1193, 1521
BpuEI CTTGAG 3 cut(s) 576, 1755, 1956
Bsa29I ATCGAT 1 cut(s) 842
BsaAI YACGTR 1 cut(s) 501
BsaBI GATNNNNATC 3 cut(s) 177, 383, 2154
BsaHI GRCGYC 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 943
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 8 cut(s) 439, 475, 1231, 1366, 1475, 1516, 1655, 1894
Bse118I RCCGGY 1 cut(s) 1742
Bse1I ACTGG 3 cut(s) 899, 1571, 1633
Bse8I GATNNNNATC 3 cut(s) 177, 383, 2154
BseBI CCWGG 4 cut(s) 612, 999, 1042, 1511
BseCI ATCGAT 1 cut(s) 842
BseGI GGATG 5 cut(s) 1240, 1693, 1993, 2035, 2092
BseJI GATNNNNATC 3 cut(s) 177, 383, 2154
BseLI CCNNNNNNNGG 8 cut(s) 439, 475, 1231, 1366, 1475, 1516, 1655, 1894
BseMII CTCAG 4 cut(s) 1074, 1392, 1431, 1512
BseNI ACTGG 3 cut(s) 899, 1571, 1633
BseRI GAGGAG 3 cut(s) 139, 1259, 2012
BseSI GKGCMC 1 cut(s) 610
BseX3I CGGCCG 1 cut(s) 1744
BseXI GCAGC 5 cut(s) 325, 328, 1703, 1729, 2118
BsgI GTGCAG 1 cut(s) 878
Bsh1285I CGRYCG 1 cut(s) 1747
BshFI GGCC 7 cut(s) 121, 130, 578, 615, 1648, 1746, 2181
BshVI ATCGAT 1 cut(s) 842
BsiEI CGRYCG 1 cut(s) 1747
BsiHKAI GWGCWC 2 cut(s) 448, 1498
BsiHKCI CYCGRG 2 cut(s) 52, 114
BsiSI CCGG 2 cut(s) 1499, 1743
BslFI GGGAC 2 cut(s) 1046, 1091
BslI CCNNNNNNNGG 8 cut(s) 439, 475, 1231, 1366, 1475, 1516, 1655, 1894
BsmAI GTCTC 5 cut(s) 672, 711, 943, 1270, 1681
BsmFI GGGAC 2 cut(s) 1046, 1091
BsmI GAATGC 2 cut(s) 1197, 1858
BsnI GGCC 7 cut(s) 121, 130, 578, 615, 1648, 1746, 2181
Bso31I GGTCTC 1 cut(s) 943
BsoBI CYCGRG 2 cut(s) 52, 114
Bsp1286I GDGCHC 4 cut(s) 67, 448, 610, 1498
Bsp143I GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
Bsp19I CCATGG 2 cut(s) 953, 2169
BspACI CCGC 6 cut(s) 297, 300, 836, 988, 1312, 1701
BspANI GGCC 7 cut(s) 121, 130, 578, 615, 1648, 1746, 2181
BspCNI CTCAG 4 cut(s) 1075, 1393, 1432, 1513
BspDI ATCGAT 1 cut(s) 842
BspLI GGNNCC 9 cut(s) 120, 995, 1079, 1080, 1153, 1591, 1741, 1899, 2182
BspMI ACCTGC 2 cut(s) 1405, 1472
BspPI GGATC 4 cut(s) 128, 980, 988, 1001
BspQI GCTCTTC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 943
BsrBI CCGCTC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 1742
BsrI ACTGG 3 cut(s) 899, 1571, 1633
BssAI RCCGGY 1 cut(s) 1742
BssMI GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
BssNI GRCGYC 1 cut(s) 304
BssT1I CCWWGG 5 cut(s) 125, 153, 653, 953, 2169
Bst2UI CCWGG 4 cut(s) 612, 999, 1042, 1511
Bst4CI ACNGT 5 cut(s) 706, 823, 1144, 1288, 1930
Bst6I CTCTTC 3 cut(s) 287, 1574, 1886
BstACI GRCGYC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 501
BstC8I GCNNGC 4 cut(s) 46, 275, 885, 1744
BstDEI CTNAG 8 cut(s) 505, 519, 1083, 1193, 1216, 1401, 1440, 1521
BstDSI CCRYGG 3 cut(s) 953, 1926, 2169
BstF5I GGATG 5 cut(s) 1240, 1693, 1993, 2035, 2092
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 8 cut(s) 136, 175, 369, 712, 975, 996, 1665, 2136
BstMAI GTCTC 5 cut(s) 672, 711, 943, 1270, 1681
BstMBI GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
BstMCI CGRYCG 1 cut(s) 1747
BstMWI GCNNNNNNNGC 3 cut(s) 54, 127, 1311
BstNI CCWGG 4 cut(s) 612, 999, 1042, 1511
BstNSI RCATGY 2 cut(s) 1332, 1343
BstSCI CCNGG 4 cut(s) 610, 997, 1040, 1509
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 5 cut(s) 325, 328, 1703, 1729, 2118
BstX2I RGATCY 2 cut(s) 972, 993
BstYI RGATCY 2 cut(s) 972, 993
BstZI CGGCCG 1 cut(s) 1744
Bsu15I ATCGAT 1 cut(s) 842
BsuRI GGCC 7 cut(s) 121, 130, 578, 615, 1648, 1746, 2181
BsuTUI ATCGAT 1 cut(s) 842
BtgI CCRYGG 3 cut(s) 953, 1926, 2169
BtrI CACGTC 1 cut(s) 1493
BtsCI GGATG 5 cut(s) 1240, 1693, 1993, 2035, 2092
BtsIMutI CAGTG 1 cut(s) 828
BveI ACCTGC 2 cut(s) 1405, 1472
Cac8I GCNNGC 4 cut(s) 46, 275, 885, 1744
CfoI GCGC 1 cut(s) 396
Cfr10I RCCGGY 1 cut(s) 1742
Cfr13I GGNCC 9 cut(s) 119, 760, 1078, 1212, 1415, 1897, 2002, 2166, 2180
ClaI ATCGAT 1 cut(s) 842
Csp6I GTAC 1 cut(s) 864
CviQI GTAC 1 cut(s) 864
DdeI CTNAG 8 cut(s) 505, 519, 1083, 1193, 1216, 1401, 1440, 1521
DpnI GATC 8 cut(s) 135, 174, 368, 711, 974, 995, 1664, 2135
DpnII GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
DrdI GACNNNNNNGTC 1 cut(s) 725
DseDI GACNNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 2 cut(s) 613, 1744
EagI CGGCCG 1 cut(s) 1744
Eam1104I CTCTTC 3 cut(s) 287, 1574, 1886
EarI CTCTTC 3 cut(s) 287, 1574, 1886
EciI GGCGGA 1 cut(s) 315
EclXI CGGCCG 1 cut(s) 1744
Eco130I CCWWGG 5 cut(s) 125, 153, 653, 953, 2169
Eco147I AGGCCT 1 cut(s) 1648
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 943
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 7 cut(s) 760, 1078, 1212, 1415, 1897, 2002, 2166
Eco52I CGGCCG 1 cut(s) 1744
Eco88I CYCGRG 2 cut(s) 52, 114
EcoO109I RGGNCCY 3 cut(s) 119, 1078, 1212
EcoRII CCWGG 4 cut(s) 610, 997, 1040, 1509
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 5 cut(s) 125, 153, 653, 953, 2169
EcoT22I ATGCAT 2 cut(s) 1341, 1860
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 5 cut(s) 125, 153, 653, 953, 2169
FaqI GGGAC 2 cut(s) 1046, 1091
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 7 cut(s) 298, 314, 317, 837, 1692, 1718, 2107
FokI GGATG 5 cut(s) 1247, 1700, 2000, 2042, 2099
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 7 cut(s) 298, 314, 317, 837, 1692, 1718, 2107
FspBI CTAG 7 cut(s) 551, 917, 1170, 1605, 1695, 2097, 2163
FspI TGCGCA 1 cut(s) 395
GlaI GCGC 1 cut(s) 395
GluI GCNGC 7 cut(s) 298, 314, 317, 837, 1692, 1718, 2107
GsuI CTGGAG 3 cut(s) 1497, 1608, 1758
HaeIII GGCC 7 cut(s) 121, 130, 578, 615, 1648, 1746, 2181
HapII CCGG 2 cut(s) 1499, 1743
HhaI GCGC 1 cut(s) 396
Hin1I GRCGYC 1 cut(s) 304
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HincII GTYRAC 1 cut(s) 1933
HindII GTYRAC 1 cut(s) 1933
HindIII AAGCTT 3 cut(s) 275, 594, 806
HinfI GANTC 9 cut(s) 379, 437, 665, 774, 1173, 1423, 1561, 1674, 2150
HpaII CCGG 2 cut(s) 1499, 1743
HphI GGTGA 6 cut(s) 701, 721, 1057, 1301, 1792, 2045
Hpy166II GTNNAC 7 cut(s) 6, 31, 760, 1255, 1284, 1490, 1933
Hpy188I TCNGA 9 cut(s) 69, 172, 285, 366, 371, 714, 1381, 1441, 1872
Hpy8I GTNNAC 7 cut(s) 6, 31, 760, 1255, 1284, 1490, 1933
HpyAV CCTTC 8 cut(s) 154, 229, 432, 826, 1268, 1346, 1898, 1929
HpyCH4III ACNGT 5 cut(s) 706, 823, 1144, 1288, 1930
HpyCH4IV ACGT 5 cut(s) 224, 304, 500, 1059, 1492
HpyCH4V TGCA 8 cut(s) 251, 273, 859, 1054, 1069, 1339, 1691, 1858
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 127, 1311
HpyF3I CTNAG 8 cut(s) 505, 519, 1083, 1193, 1216, 1401, 1440, 1521
HpySE526I ACGT 5 cut(s) 224, 304, 500, 1059, 1492
Hsp92I GRCGYC 1 cut(s) 304
HspAI GCGC 1 cut(s) 394
KflI GGGWCCC 1 cut(s) 1078
KroI GCCGGC 1 cut(s) 1742
KroNI GCCGGC 1 cut(s) 1744
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 7 cut(s) 443, 1183, 1478, 1501, 1589, 1627, 1739
Lsp1109I GCAGC 5 cut(s) 325, 328, 1703, 1729, 2118
LweI GCATC 5 cut(s) 532, 802, 848, 1678, 2032
MaeI CTAG 7 cut(s) 551, 917, 1170, 1605, 1695, 2097, 2163
MaeII ACGT 5 cut(s) 224, 304, 500, 1059, 1492
MaeIII GTNAC 4 cut(s) 187, 635, 727, 1532
MalI GATC 8 cut(s) 135, 174, 368, 711, 974, 995, 1664, 2135
MbiI CCGCTC 1 cut(s) 297
MboI GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
MfeI CAATTG 1 cut(s) 1710
MflI RGATCY 2 cut(s) 972, 993
MhlI GDGCHC 4 cut(s) 67, 448, 610, 1498
MlsI TGGCCA 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 3 cut(s) 674, 1182, 1683
Mox20I TGGCCA 1 cut(s) 615
Mph1103I ATGCAT 2 cut(s) 1341, 1860
MroNI GCCGGC 1 cut(s) 1742
MscI TGGCCA 1 cut(s) 615
MseI TTAA 9 cut(s) 15, 162, 279, 1125, 1206, 1683, 1835, 1941, 2114
MslI CAYNNNNRTG 3 cut(s) 180, 844, 1950
Msp20I TGGCCA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 988
MspI CCGG 2 cut(s) 1499, 1743
MspR9I CCNGG 4 cut(s) 612, 999, 1042, 1511
MunI CAATTG 1 cut(s) 1710
Mva1269I GAATGC 2 cut(s) 1197, 1858
MvaI CCWGG 4 cut(s) 612, 999, 1042, 1511
MwoI GCNNNNNNNGC 3 cut(s) 54, 127, 1311
NaeI GCCGGC 1 cut(s) 1744
NcoI CCATGG 2 cut(s) 953, 2169
NdeII GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
NgoMIV GCCGGC 1 cut(s) 1742
NlaIV GGNNCC 9 cut(s) 120, 995, 1079, 1080, 1153, 1591, 1741, 1899, 2182
NmuCI GTSAC 3 cut(s) 187, 727, 1532
NsbI TGCGCA 1 cut(s) 395
NsiI ATGCAT 2 cut(s) 1341, 1860
NspI RCATGY 2 cut(s) 1332, 1343
PaeR7I CTCGAG 1 cut(s) 52
PceI AGGCCT 1 cut(s) 1648
PciSI GCTCTTC 1 cut(s) 287
PctI GAATGC 2 cut(s) 1197, 1858
PdiI GCCGGC 1 cut(s) 1744
PfeI GAWTC 6 cut(s) 379, 437, 774, 1423, 1561, 2150
PkrI GCNGC 7 cut(s) 299, 315, 318, 838, 1693, 1719, 2108
PleI GAGTC 3 cut(s) 673, 1181, 1682
PpsI GAGTC 3 cut(s) 673, 1181, 1682
Ppu21I YACGTR 1 cut(s) 501
PpuMI RGGWCCY 2 cut(s) 1078, 1212
Psp5II RGGWCCY 2 cut(s) 1078, 1212
Psp6I CCWGG 4 cut(s) 610, 997, 1040, 1509
PspGI CCWGG 4 cut(s) 610, 997, 1040, 1509
PspN4I GGNNCC 9 cut(s) 120, 995, 1079, 1080, 1153, 1591, 1741, 1899, 2182
PspPI GGNCC 9 cut(s) 119, 760, 1078, 1212, 1415, 1897, 2002, 2166, 2180
PspPPI RGGWCCY 2 cut(s) 1078, 1212
PsuI RGATCY 2 cut(s) 972, 993
RsaI GTAC 1 cut(s) 865
RsaNI GTAC 1 cut(s) 864
RseI CAYNNNNRTG 3 cut(s) 180, 844, 1950
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 9 cut(s) 15, 162, 279, 1125, 1206, 1683, 1835, 1941, 2114
SatI GCNGC 7 cut(s) 298, 314, 317, 837, 1692, 1718, 2107
Sau3AI GATC 8 cut(s) 133, 172, 366, 709, 972, 993, 1662, 2133
Sau96I GGNCC 9 cut(s) 119, 760, 1078, 1212, 1415, 1897, 2002, 2166, 2180
SchI GAGTC 3 cut(s) 674, 1182, 1683
ScrFI CCNGG 4 cut(s) 612, 999, 1042, 1511
SduI GDGCHC 4 cut(s) 67, 448, 610, 1498
SfaNI GCATC 5 cut(s) 532, 802, 848, 1678, 2032
SfiI GGCCNNNNNGGCC 1 cut(s) 127
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 7 cut(s) 760, 1078, 1212, 1415, 1897, 2002, 2166
SlaI CTCGAG 1 cut(s) 52
SmiMI CAYNNNNRTG 3 cut(s) 180, 844, 1950
SmlI CTYRAG 4 cut(s) 52, 591, 1770, 1971
SmoI CTYRAG 4 cut(s) 52, 591, 1770, 1971
SpeI ACTAGT 1 cut(s) 1604
SseBI AGGCCT 1 cut(s) 1648
SsiI CCGC 6 cut(s) 297, 300, 836, 988, 1312, 1701
SspMI CTAG 7 cut(s) 551, 917, 1170, 1605, 1695, 2097, 2163
StuI AGGCCT 1 cut(s) 1648
StyD4I CCNGG 4 cut(s) 610, 997, 1040, 1509
StyI CCWWGG 5 cut(s) 125, 153, 653, 953, 2169
TaaI ACNGT 5 cut(s) 706, 823, 1144, 1288, 1930
TaiI ACGT 5 cut(s) 227, 307, 503, 1062, 1495
TaqI TCGA 6 cut(s) 24, 53, 435, 772, 842, 2136
TauI GCSGC 2 cut(s) 300, 839
TfiI GAWTC 6 cut(s) 379, 437, 774, 1423, 1561, 2150
Tru1I TTAA 9 cut(s) 15, 162, 279, 1125, 1206, 1683, 1835, 1941, 2114
Tru9I TTAA 9 cut(s) 15, 162, 279, 1125, 1206, 1683, 1835, 1941, 2114
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 3 cut(s) 187, 727, 1532
TseI GCWGC 5 cut(s) 313, 316, 1691, 1717, 2106
Tsp45I GTSAC 3 cut(s) 187, 727, 1532
TspDTI ATGAA 8 cut(s) 371, 1287, 1365, 1401, 1838, 1952, 2094, 2157
TspRI CASTG 1 cut(s) 828
VpaK11BI GGWCC 7 cut(s) 760, 1078, 1212, 1415, 1897, 2002, 2166
XapI RAATTY 5 cut(s) 264, 452, 792, 1118, 1903
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 2 cut(s) 1332, 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 1071
XhoI CTCGAG 1 cut(s) 52
XspI CTAG 7 cut(s) 551, 917, 1170, 1605, 1695, 2097, 2163
ZraI GACGTC 1 cut(s) 305
Zsp2I ATGCAT 2 cut(s) 1341, 1860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.