MD10G1126900.v1.1

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000182
Physical Location & Seq
Forward (+)
20675724 .. 20676640
917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1126900.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGTTTTGGCTTGTTGGCTTGTGCCGTTGGGCTGGTGTTTTTGGAGTTTTTATGCCTCAGGTTAGAGTGTGCCACCTTTACTCCTAGTAATTGGCTGAGTTTTGGTTTTATGACCTATGGTTGTGCTTTGATGGCTGGTTTCCCCTTAAGTGCCTTTATATATTCCGGTTTTGGTGGTGTCTTCTCAACACATCAAAATTCTTGTGTTTCCTCTCCCTTGTTTTCTAGAATTCTCAAAATGACTAACCTTCAAATTACTACTTCATCCTCTAACAATGCTCGTAGGCCTAAAATCTGTTCTAAGGGTTATATCCCAAGCTGGGAGAATCCTTTCCCTATTCCCCAGCCTGGTCAAGACCTGGTTGTTGTTGAAAAGGCTATGAGCACGTTCACTATGGAATGTGGAGTGACTAGGATGAATACCCATTTATGCTCTTCTATTAATGTTGGGCACATGCTATCTAGGGTTCTTATAGGGAAGATGTTTGGCCAACCTCTTGATGCTCGTTTGATTAAGTGGAAGTTAGGGTTTCTATGGAAGAAAGAGGTGAAGAACACCTTTTATTTGGATCACTTTGGAAGGAAGTGGTTTGCACTCGAATTCACTGATGAGGATGATTTGAAATTCGTCCTGAAGAATCGACCATGGTACATGCGTGGTCAAAATTTTCACCTTGAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

229

Amino Acids

25.96

Weight (kDa)

9.27

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 154 - 228 4.8e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000182)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21471 FvH4_1g27953 FvH4_1g28801 FvH4_2g14522 FvH4_3g04280 FvH4_3g28251 FvH4_4g07491 FvH4_4g30262
malus_domestica MD10G1046400.v1.1 MD10G1126900.v1.1 MD11G1236100.v1.1
prunus_persica Prupe.1G148300_v2.0.a1 Prupe.2G036800_v2.0.a1 Prupe.8G054600_v2.0.a1 Prupe.8G054700_v2.0.a1
pyrus_communis pycom05g05520 pycom08g11160 pycom13g29480
rosa_chinensis RchiOBHm_Chr7g0182921 RchiOBHm_Chr7g0230111
rosa_laevigata RLG00000000536 RLG00000001385 RLG00000001492 RLG00000002640 RLG00000003082 RLG00000005042 RLG00000007254 RLG00000007773 RLG00000008594 RLG00000009508 RLG00000010355 RLG00000013369 RLG00000014370 RLG00000014371 RLG00000018745 RLG00000020268 RLG00000020832 RLG00000022569 RLG00000028073 RLG00000028388 RLG00000028677 RLG00000035645
rosa_multiflora Rmu_co8277499.1_g000001 Rmu_co8500999.1_g000001 Rmu_sc0000014.1_g000025 Rmu_sc0000103.1_g000003 Rmu_sc0000168.1_g000019 Rmu_sc0000283.1_g000006 Rmu_sc0000693.1_g000080 Rmu_sc0000997.1_g000005 Rmu_sc0001228.1_g000005 Rmu_sc0001231.1_g000023 Rmu_sc0002833.1_g000037 Rmu_sc0002869.1_g000015 Rmu_sc0003545.1_g000003 Rmu_sc0003693.1_g000028 Rmu_sc0004000.1_g000022 Rmu_sc0004454.1_g000007 Rmu_sc0004976.1_g000036 Rmu_sc0005014.1_g000013 Rmu_sc0005046.1_g000013 Rmu_sc0005063.1_g000009 Rmu_sc0005652.1_g000002 Rmu_sc0005697.1_g000008 Rmu_sc0006989.1_g000014 Rmu_sc0008957.1_g000003 Rmu_sc0011659.1_g000001 Rmu_sc0027477.1_g000001 Rmu_ssc0000387.1_g000021
rosa_roxburghii Rroxscaffold_1G00020860 Rroxscaffold_1G00035780 Rroxscaffold_1G00051370 Rroxscaffold_2G00101730 Rroxscaffold_3G00230130 Rroxscaffold_4G00280070 Rroxscaffold_4G00281160 Rroxscaffold_4G00318780 Rroxscaffold_5G00341570 Rroxscaffold_5G00341580 Rroxscaffold_7G00191230 Rroxscaffold_7G00195880 Rroxscaffold_7G00195890 Rroxscaffold_7G00209080 Rroxscaffold_7G00209090
rosa_rugosa Rorug02G0170400 Rorug03G0234500 Rorug04G0045200 Rorug07G0191000
rosa_samantha Rh1AG012200 Rh1AG012300 Rh1AG021700 Rh1AG033800 Rh1AG033900 Rh1AG048100 Rh1AG087000 Rh1AG168600 Rh1CG004600 Rh2BG192700 Rh2BG200800 Rh2BG310200 Rh2BG356900 Rh2BG377900 Rh2BG378000 Rh2CG356300 Rh2DG394800 Rh3AG199000 Rh3AG258700 Rh3AG258800 Rh3AG324400 Rh3BG202300 Rh3BG202400 Rh3BG228800 Rh3BG318800 Rh3BG360400 Rh3BG368300 Rh3BG373700 Rh4AG071400 Rh4AG153700 Rh4AG224300 Rh4AG224400 Rh4CG044000 Rh4CG077200 Rh4CG077300 Rh4CG238700 Rh4CG238800 Rh4CG238900 Rh5BG367100 Rh5BG379500 Rh5BG379600 Rh5BG396700 Rh5BG412900 Rh5DG427200 Rh6AG015200 Rh6AG015300 Rh6AG122800 Rh6AG175600 Rh6AG187700 Rh6AG187800 Rh6AG260800 Rh6AG355000 Rh6BG074900 Rh6BG102300 Rh7AG127400 Rh7AG157500 Rh7AG157600 Rh7AG212200 Rh7AG331400 Rh7AG412000 Rh7CG055400 Rh7CG131400 Rh7CG131500 Rh7CG224300 Rh7CG267100 Rh7CG348800
rosa_wichuraiana Rw0G009820 Rw1G000520 Rw1G008820 Rw4G014110 Rw4G019370 Rw4G022440 Rw6G010550 Rw7G001520 Rw7G018400 Rw7G025210 Rw7G027910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 579
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 5 cut(s) 199, 231, 602, 626, 667
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 1 cut(s) 653
AfiI CCNNNNNNNGG 2 cut(s) 322, 350
AflII CTTAAG 1 cut(s) 148
AgsI TTSAA 4 cut(s) 254, 374, 625, 680
AjnI CCWGG 2 cut(s) 349, 360
AluBI AGCT 1 cut(s) 321
AluI AGCT 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 389
AlwI GGATC 1 cut(s) 579
AoxI GGCC 2 cut(s) 287, 490
ApoI RAATTY 5 cut(s) 199, 231, 602, 626, 667
AseI ATTAAT 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 562, 665
AxyI CCTNAGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 643, 676
BalI TGGCCA 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 389
BccI CCATC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 2 cut(s) 351, 362
BfaI CTAG 4 cut(s) 87, 228, 414, 465
BfrI CTTAAG 1 cut(s) 148
Bme1390I CCNGG 2 cut(s) 351, 362
BmrFI CCNGG 2 cut(s) 351, 362
BmsI GCATC 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 647
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 350
Bse21I CCTNAGG 1 cut(s) 59
BseBI CCWGG 2 cut(s) 351, 362
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 3 cut(s) 266, 423, 622
BseLI CCNNNNNNNGG 2 cut(s) 322, 350
BseMII CTCAG 2 cut(s) 73, 89
BseSI GKGCMC 1 cut(s) 456
BseYI CCCAGC 2 cut(s) 321, 345
BshFI GGCC 2 cut(s) 289, 492
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 322, 350
BsnI GGCC 2 cut(s) 289, 492
Bsp1286I GDGCHC 2 cut(s) 389, 456
Bsp143I GATC 1 cut(s) 571
Bsp19I CCATGG 1 cut(s) 647
BspANI GGCC 2 cut(s) 289, 492
BspCNI CTCAG 2 cut(s) 72, 90
BspPI GGATC 1 cut(s) 579
BspQI GCTCTTC 1 cut(s) 442
BspTI CTTAAG 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 1 cut(s) 571
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 2 cut(s) 351, 362
Bst4CI ACNGT 1 cut(s) 684
Bst6I CTCTTC 1 cut(s) 442
BstAFI CTTAAG 1 cut(s) 148
BstDEI CTNAG 3 cut(s) 59, 98, 303
BstDSI CCRYGG 1 cut(s) 647
BstF5I GGATG 3 cut(s) 266, 423, 622
BstKTI GATC 1 cut(s) 574
BstMBI GATC 1 cut(s) 571
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 2 cut(s) 351, 362
BstNSI RCATGY 2 cut(s) 460, 658
BstSCI CCNGG 2 cut(s) 349, 360
BstSLI GKGCMC 1 cut(s) 456
BstV2I GAAGAC 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 59
BsuRI GGCC 2 cut(s) 289, 492
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 3 cut(s) 266, 423, 622
BtsIMutI CAGTG 1 cut(s) 606
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 652
CviAII CATG 3 cut(s) 457, 648, 655
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 3 cut(s) 59, 98, 303
DpnI GATC 1 cut(s) 573
DpnII GATC 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 59
EcoRI GAATTC 2 cut(s) 231, 602
EcoRII CCWGG 2 cut(s) 349, 360
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 3 cut(s) 460, 651, 658
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FatI CATG 3 cut(s) 456, 647, 654
FokI GGATG 3 cut(s) 253, 430, 629
FspBI CTAG 4 cut(s) 87, 228, 414, 465
GsaI CCCAGC 2 cut(s) 325, 349
HaeIII GGCC 2 cut(s) 289, 492
HapII CCGG 1 cut(s) 168
Hin1II CATG 3 cut(s) 460, 651, 658
HinfI GANTC 2 cut(s) 328, 640
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 562, 665
Hpy166II GTNNAC 1 cut(s) 393
Hpy188III TCNNGA 4 cut(s) 228, 356, 500, 634
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 260, 576
HpyCH4III ACNGT 1 cut(s) 684
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 59, 98, 303
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 3 cut(s) 460, 651, 658
Kzo9I GATC 1 cut(s) 571
LguI GCTCTTC 1 cut(s) 442
LweI GCATC 1 cut(s) 493
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 4 cut(s) 87, 228, 414, 465
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 409
MalI GATC 1 cut(s) 573
MboI GATC 1 cut(s) 571
MboII GAAGA 6 cut(s) 175, 429, 493, 553, 565, 649
MhlI GDGCHC 2 cut(s) 389, 456
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 7 cut(s) 91, 199, 231, 255, 602, 626, 667
MluNI TGGCCA 1 cut(s) 492
MnlI CCTC 6 cut(s) 68, 223, 280, 507, 541, 607
Mox20I TGGCCA 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 492
MseI TTAA 3 cut(s) 149, 444, 516
Msp20I TGGCCA 1 cut(s) 492
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 2 cut(s) 351, 362
MvaI CCWGG 2 cut(s) 351, 362
MwoI GCNNNNNNNGC 1 cut(s) 134
NcoI CCATGG 1 cut(s) 647
NdeII GATC 1 cut(s) 571
NlaIII CATG 3 cut(s) 460, 651, 658
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 2 cut(s) 460, 658
PceI AGGCCT 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 328, 640
PshBI ATTAAT 1 cut(s) 444
Psp6I CCWGG 2 cut(s) 349, 360
PspFI CCCAGC 2 cut(s) 321, 345
PspGI CCWGG 2 cut(s) 349, 360
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 3 cut(s) 149, 444, 516
Sau3AI GATC 1 cut(s) 571
ScrFI CCNGG 2 cut(s) 351, 362
SduI GDGCHC 2 cut(s) 389, 456
SexAI ACCWGGT 1 cut(s) 360
SfaNI GCATC 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 7 cut(s) 91, 199, 231, 255, 602, 626, 667
SseBI AGGCCT 1 cut(s) 289
SspMI CTAG 4 cut(s) 87, 228, 414, 465
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 2 cut(s) 349, 360
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 1 cut(s) 684
TaiI ACGT 1 cut(s) 392
TaqI TCGA 2 cut(s) 600, 643
TasI AATT 7 cut(s) 91, 199, 231, 255, 602, 626, 667
TfiI GAWTC 2 cut(s) 328, 640
Tru1I TTAA 3 cut(s) 149, 444, 516
Tru9I TTAA 3 cut(s) 149, 444, 516
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 255, 434
TspRI CASTG 1 cut(s) 613
Vha464I CTTAAG 1 cut(s) 148
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 5 cut(s) 199, 231, 602, 626, 667
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 2 cut(s) 460, 658
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 4 cut(s) 87, 228, 414, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.