pycom05g05520

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000182
Physical Location & Seq
Reverse (-)
6688361 .. 6689665
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g05520.1

Sequence Viewer

Length: 711 bp
ATGGAAGGTCTAGGGTTTCTGCATGCAAGGTGCTGCCGGCGTCCTAGCGCTGGTCTCTTGTGCGTGTCCTCTAGGTTTTTCAGTTGGGTTAGAGATAATTTCATTAGGGGTAAGCAGATCTCTGTTGATTACATACTAGGTGAGTGCTTGCTTACCCTAATTAGGTTTTCGATAATTGGAAATAGGTTAATCATGAGTTCGAATAGGTTGGGGTGGAGTGCTAATTGGTTGCTAGATAAGTGGGTGGTGAGCTCCGAGGAGTCGTTTGAACTGATAGTTCTCAAAATGACTAACCCTCAGCTTGCTGCTTCCTCCTCTAGTAATGCCCGTAGGCATAGAATTTGTTTTGAGGGTTATATCTCGAACTGGGAGAATCCTTTCCTTGTTCCACAACCTGGTCATGACCCAGTTGTTGTTGAAAAGGCTATGAGCACGTTCACTGTGGAACGTGGAGTGACCAGGTTGAATACCCATTTATTCTTTTCTATTAATGTTGAGCACACGTTATCTAGGGTTCTTATGGGGAAGATGTTCGGCCAACCTTTTAATGCTCGTTTGATTAAGTGGAAGCTGAGGCTTCTGTGGAAGAATGAGGTGAATAACACCTTTTATTTGGATCACTTCGGAAGGAAATGGTTTGCACTCGAATTCACTGATGAGGATGATTTGAAATTCGTCTTGAAGAATCGACCATGGTACGTCCGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

237

Amino Acids

27.56

Weight (kDa)

9.67

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000182)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21471 FvH4_1g27953 FvH4_1g28801 FvH4_2g14522 FvH4_3g04280 FvH4_3g28251 FvH4_4g07491 FvH4_4g30262
malus_domestica MD10G1046400.v1.1 MD10G1126900.v1.1 MD11G1236100.v1.1
prunus_persica Prupe.1G148300_v2.0.a1 Prupe.2G036800_v2.0.a1 Prupe.8G054600_v2.0.a1 Prupe.8G054700_v2.0.a1
pyrus_communis pycom05g05520 pycom08g11160 pycom13g29480
rosa_chinensis RchiOBHm_Chr7g0182921 RchiOBHm_Chr7g0230111
rosa_laevigata RLG00000000536 RLG00000001385 RLG00000001492 RLG00000002640 RLG00000003082 RLG00000005042 RLG00000007254 RLG00000007773 RLG00000008594 RLG00000009508 RLG00000010355 RLG00000013369 RLG00000014370 RLG00000014371 RLG00000018745 RLG00000020268 RLG00000020832 RLG00000022569 RLG00000028073 RLG00000028388 RLG00000028677 RLG00000035645
rosa_multiflora Rmu_co8277499.1_g000001 Rmu_co8500999.1_g000001 Rmu_sc0000014.1_g000025 Rmu_sc0000103.1_g000003 Rmu_sc0000168.1_g000019 Rmu_sc0000283.1_g000006 Rmu_sc0000693.1_g000080 Rmu_sc0000997.1_g000005 Rmu_sc0001228.1_g000005 Rmu_sc0001231.1_g000023 Rmu_sc0002833.1_g000037 Rmu_sc0002869.1_g000015 Rmu_sc0003545.1_g000003 Rmu_sc0003693.1_g000028 Rmu_sc0004000.1_g000022 Rmu_sc0004454.1_g000007 Rmu_sc0004976.1_g000036 Rmu_sc0005014.1_g000013 Rmu_sc0005046.1_g000013 Rmu_sc0005063.1_g000009 Rmu_sc0005652.1_g000002 Rmu_sc0005697.1_g000008 Rmu_sc0006989.1_g000014 Rmu_sc0008957.1_g000003 Rmu_sc0011659.1_g000001 Rmu_sc0027477.1_g000001 Rmu_ssc0000387.1_g000021
rosa_roxburghii Rroxscaffold_1G00020860 Rroxscaffold_1G00035780 Rroxscaffold_1G00051370 Rroxscaffold_2G00101730 Rroxscaffold_3G00230130 Rroxscaffold_4G00280070 Rroxscaffold_4G00281160 Rroxscaffold_4G00318780 Rroxscaffold_5G00341570 Rroxscaffold_5G00341580 Rroxscaffold_7G00191230 Rroxscaffold_7G00195880 Rroxscaffold_7G00195890 Rroxscaffold_7G00209080 Rroxscaffold_7G00209090
rosa_rugosa Rorug02G0170400 Rorug03G0234500 Rorug04G0045200 Rorug07G0191000
rosa_samantha Rh1AG012200 Rh1AG012300 Rh1AG021700 Rh1AG033800 Rh1AG033900 Rh1AG048100 Rh1AG087000 Rh1AG168600 Rh1CG004600 Rh2BG192700 Rh2BG200800 Rh2BG310200 Rh2BG356900 Rh2BG377900 Rh2BG378000 Rh2CG356300 Rh2DG394800 Rh3AG199000 Rh3AG258700 Rh3AG258800 Rh3AG324400 Rh3BG202300 Rh3BG202400 Rh3BG228800 Rh3BG318800 Rh3BG360400 Rh3BG368300 Rh3BG373700 Rh4AG071400 Rh4AG153700 Rh4AG224300 Rh4AG224400 Rh4CG044000 Rh4CG077200 Rh4CG077300 Rh4CG238700 Rh4CG238800 Rh4CG238900 Rh5BG367100 Rh5BG379500 Rh5BG379600 Rh5BG396700 Rh5BG412900 Rh5DG427200 Rh6AG015200 Rh6AG015300 Rh6AG122800 Rh6AG175600 Rh6AG187700 Rh6AG187800 Rh6AG260800 Rh6AG355000 Rh6BG074900 Rh6BG102300 Rh7AG127400 Rh7AG157500 Rh7AG157600 Rh7AG212200 Rh7AG331400 Rh7AG412000 Rh7CG055400 Rh7CG131400 Rh7CG131500 Rh7CG224300 Rh7CG267100 Rh7CG348800
rosa_wichuraiana Rw0G009820 Rw1G000520 Rw1G008820 Rw4G014110 Rw4G019370 Rw4G022440 Rw6G010550 Rw7G001520 Rw7G018400 Rw7G025210 Rw7G027910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 395
AclWI GGATC 1 cut(s) 624
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 3 cut(s) 339, 647, 671
AcyI GRCGYC 1 cut(s) 40
AfaI GTAC 1 cut(s) 698
AfeI AGCGCT 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 50, 162, 395
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 5 cut(s) 269, 419, 466, 670, 682
AjnI CCWGG 2 cut(s) 394, 458
AluBI AGCT 3 cut(s) 252, 301, 571
AluI AGCT 3 cut(s) 252, 301, 571
Alw21I GWGCWC 3 cut(s) 254, 434, 501
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 1 cut(s) 624
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 2 cut(s) 33, 305
ApoI RAATTY 3 cut(s) 339, 647, 671
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 2 cut(s) 377, 530
AspLEI GCGC 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 152, 259, 607
AsuII TTCGAA 1 cut(s) 200
BaeI ACNNNNGTAYC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 254
Bbv12I GWGCWC 3 cut(s) 254, 434, 501
BbvCI CCTCAGC 2 cut(s) 297, 572
BbvI GCAGC 2 cut(s) 20, 292
BciT130I CCWGG 2 cut(s) 396, 460
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 7 cut(s) 11, 45, 72, 137, 233, 318, 510
BfoI RGCGCY 1 cut(s) 51
BglII AGATCT 1 cut(s) 117
BisI GCNGC 2 cut(s) 34, 306
BlsI GCNGC 2 cut(s) 35, 307
Bme1390I CCNGG 2 cut(s) 396, 460
BmrFI CCNGG 2 cut(s) 396, 460
BmrI ACTGGG 2 cut(s) 376, 401
BmuI ACTGGG 2 cut(s) 376, 401
Bpu10I CCTNAGC 2 cut(s) 297, 572
Bpu14I TTCGAA 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 40
BsaI GGTCTC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 255, 692, 703
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 162, 395
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 2 cut(s) 371, 407
BseBI CCWGG 2 cut(s) 396, 460
BseDI CCNNGG 3 cut(s) 255, 692, 703
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 3 cut(s) 50, 162, 395
BseMII CTCAG 2 cut(s) 311, 563
BseNI ACTGG 2 cut(s) 371, 407
BseRI GAGGAG 2 cut(s) 272, 304
BseXI GCAGC 2 cut(s) 20, 292
BshFI GGCC 1 cut(s) 537
BsiHKAI GWGCWC 3 cut(s) 254, 434, 501
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 50, 162, 395
BsmAI GTCTC 1 cut(s) 59
BsnI GGCC 1 cut(s) 537
Bso31I GGTCTC 1 cut(s) 59
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 3 cut(s) 254, 434, 501
Bsp143I GATC 2 cut(s) 117, 616
Bsp19I CCATGG 1 cut(s) 692
BspANI GGCC 1 cut(s) 537
BspCNI CTCAG 2 cut(s) 310, 564
BspHI TCATGA 2 cut(s) 192, 400
BspPI GGATC 1 cut(s) 624
BspT104I TTCGAA 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 2 cut(s) 371, 407
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 3 cut(s) 255, 692, 703
BssMI GATC 2 cut(s) 117, 616
BssNI GRCGYC 1 cut(s) 40
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 2 cut(s) 396, 460
Bst4CI ACNGT 1 cut(s) 442
BstACI GRCGYC 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 4 cut(s) 24, 38, 149, 303
BstDEI CTNAG 2 cut(s) 297, 572
BstDSI CCRYGG 2 cut(s) 692, 703
BstF5I GGATG 1 cut(s) 667
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 2 cut(s) 120, 619
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 2 cut(s) 117, 616
BstNI CCWGG 2 cut(s) 396, 460
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 2 cut(s) 394, 458
BstV1I GCAGC 2 cut(s) 20, 292
BstX2I RGATCY 1 cut(s) 117
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 1 cut(s) 537
BtgI CCRYGG 2 cut(s) 692, 703
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 2 cut(s) 438, 651
Cac8I GCNNGC 4 cut(s) 24, 38, 149, 303
CciI TCATGA 2 cut(s) 192, 400
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 36
CseI GACGC 1 cut(s) 29
CsiI ACCWGGT 2 cut(s) 394, 458
Csp6I GTAC 1 cut(s) 697
CviAII CATG 4 cut(s) 23, 193, 401, 693
CviJI RGCY 6 cut(s) 252, 301, 425, 537, 571, 577
CviKI_1 RGCY 6 cut(s) 252, 301, 425, 537, 571, 577
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 2 cut(s) 297, 572
DpnI GATC 2 cut(s) 119, 618
DpnII GATC 2 cut(s) 117, 616
EaeI YGGCCR 1 cut(s) 535
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 692
Eco24I GRGCYC 1 cut(s) 254
Eco31I GGTCTC 1 cut(s) 59
Eco47III AGCGCT 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 394, 458
EcoT14I CCWWGG 1 cut(s) 692
EcoT38I GRGCYC 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 692
FaeI CATG 4 cut(s) 26, 196, 404, 696
FaiI YATR 9 cut(s) 24, 134, 194, 336, 357, 402, 428, 521, 694
FatI CATG 4 cut(s) 22, 192, 400, 692
Fnu4HI GCNGC 2 cut(s) 34, 306
FokI GGATG 1 cut(s) 674
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 34, 306
FspBI CTAG 7 cut(s) 11, 45, 72, 137, 233, 318, 510
GlaI GCGC 1 cut(s) 49
GluI GCNGC 2 cut(s) 34, 306
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 537
HapII CCGG 1 cut(s) 37
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 40
Hin1II CATG 4 cut(s) 26, 196, 404, 696
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 3 cut(s) 260, 373, 685
HpaII CCGG 1 cut(s) 37
HphI GGTGA 3 cut(s) 152, 259, 607
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 2 cut(s) 256, 626
Hpy188III TCNNGA 4 cut(s) 193, 361, 401, 679
Hpy8I GTNNAC 1 cut(s) 438
HpyAV CCTTC 1 cut(s) 621
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 4 cut(s) 434, 448, 503, 699
HpyCH4V TGCA 3 cut(s) 22, 26, 641
HpyF3I CTNAG 2 cut(s) 297, 572
HpySE526I ACGT 4 cut(s) 434, 448, 503, 699
Hsp92I GRCGYC 1 cut(s) 40
Hsp92II CATG 4 cut(s) 26, 196, 404, 696
HspAI GCGC 1 cut(s) 48
KroI GCCGGC 1 cut(s) 36
KroNI GCCGGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 117, 616
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 8 cut(s) 36, 50, 352, 381, 408, 420, 445, 472
Lsp1109I GCAGC 2 cut(s) 20, 292
MabI ACCWGGT 2 cut(s) 394, 458
MaeI CTAG 7 cut(s) 11, 45, 72, 137, 233, 318, 510
MaeII ACGT 4 cut(s) 434, 448, 503, 699
MaeIII GTNAC 1 cut(s) 454
MalI GATC 2 cut(s) 119, 618
MboI GATC 2 cut(s) 117, 616
MboII GAAGA 3 cut(s) 538, 598, 694
MflI RGATCY 1 cut(s) 117
MhlI GDGCHC 3 cut(s) 254, 434, 501
MluCI AATT 7 cut(s) 97, 159, 174, 223, 339, 647, 671
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 9 cut(s) 79, 250, 306, 322, 325, 343, 567, 586, 652
MroNI GCCGGC 1 cut(s) 36
MroXI GAANNNNTTC 2 cut(s) 377, 530
MseI TTAA 5 cut(s) 188, 489, 546, 561, 709
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 396, 460
MvaI CCWGG 2 cut(s) 396, 460
NaeI GCCGGC 1 cut(s) 38
NcoI CCATGG 1 cut(s) 692
NdeII GATC 2 cut(s) 117, 616
NgoMIV GCCGGC 1 cut(s) 36
NlaIII CATG 4 cut(s) 26, 196, 404, 696
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 26
NspV TTCGAA 1 cut(s) 200
PaeI GCATGC 1 cut(s) 26
PagI TCATGA 2 cut(s) 192, 400
PdiI GCCGGC 1 cut(s) 38
PdmI GAANNNNTTC 2 cut(s) 377, 530
PfeI GAWTC 2 cut(s) 373, 685
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 2 cut(s) 35, 307
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 394, 458
PspGI CCWGG 2 cut(s) 394, 458
PsuI RGATCY 1 cut(s) 117
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 5 cut(s) 188, 489, 546, 561, 709
SatI GCNGC 2 cut(s) 34, 306
Sau3AI GATC 2 cut(s) 117, 616
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 396, 460
SduI GDGCHC 3 cut(s) 254, 434, 501
SexAI ACCWGGT 2 cut(s) 394, 458
SfuI TTCGAA 1 cut(s) 200
SphI GCATGC 1 cut(s) 26
Sse9I AATT 7 cut(s) 97, 159, 174, 223, 339, 647, 671
SspMI CTAG 7 cut(s) 11, 45, 72, 137, 233, 318, 510
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 2 cut(s) 394, 458
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 4 cut(s) 437, 451, 506, 702
TaqI TCGA 5 cut(s) 170, 200, 362, 645, 688
TasI AATT 7 cut(s) 97, 159, 174, 223, 339, 647, 671
TfiI GAWTC 2 cut(s) 373, 685
Tru1I TTAA 5 cut(s) 188, 489, 546, 561, 709
Tru9I TTAA 5 cut(s) 188, 489, 546, 561, 709
TscAI CASTG 2 cut(s) 445, 658
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 33, 305
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 1 cut(s) 91
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 2 cut(s) 445, 658
Van91I CCANNNNNTGG 1 cut(s) 395
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 3 cut(s) 339, 647, 671
XceI RCATGY 1 cut(s) 26
XmnI GAANNNNTTC 2 cut(s) 377, 530
XspI CTAG 7 cut(s) 11, 45, 72, 137, 233, 318, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.