FvH4_5g35254

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000101
Physical Location & Seq
Reverse (-)
25826090 .. 25826647
558 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35254.t1

Sequence Viewer

Length: 516 bp
ATGGAGGACACTAGTCTCCCCAATTCTTCAGCCGATGACCTCAAGAAACATGTGATGAAGGTTTTCGAGGAGGCCACCGAAGACCGTGAGGACCTTTCCTTGAAAGGAATGTTTGACTCGATCAGGGACTACGACTCCCTTAACAACCTTGCTGATGAAACCAAAGAGCTCTTTAAGCCTCAGCCTGAGTCGGGCATTCTCACTGACGTTGCCGTCTTCACTATTGTCATTTGGGCCTACACCTATGTCGACAAGACCGAGGCTGCTCTCAAGGTATACCGCCAGATGATAGCCGCCAGCGTCTCCCCCACTTCTTACACATACATTATACTCATTACCTCACTTGCAACACACTCGTCTATTGATGTCAGTTTTCTTGGGTATGCCAAGAAGTACTTTTTGGAAATGTTGGATAAGGGGATGAAGCTCCATTCTGCCACCTACACCACTGTGTTAGATGCCATTGCCTGCCGTGAGTCGGCAGAGAAGGCCAGGGAGTTTGTTGAGCAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

172

Amino Acids

19.31

Weight (kDa)

4.74

Isoelectric Point (pI)

27.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 69 - 116 3.7e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28420
fragaria_vesca FvH4_3g36751 FvH4_4g16590 FvH4_4g16590 FvH4_4g16590 FvH4_5g05670 FvH4_5g05700 FvH4_5g31002 FvH4_5g31070 FvH4_5g31080 FvH4_5g31080 FvH4_5g31080 FvH4_5g31150 FvH4_5g32070 FvH4_5g32080 FvH4_5g34900 FvH4_5g34930 FvH4_5g34930 FvH4_5g34950 FvH4_5g35000 FvH4_5g35000 FvH4_5g35000 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35060 FvH4_5g35070 FvH4_5g35071 FvH4_5g35080 FvH4_5g35253 FvH4_5g35254 FvH4_5g35300 FvH4_5g35300 FvH4_5g35320 FvH4_5g35320 FvH4_5g35320 FvH4_5g35322 FvH4_5g35330 FvH4_6g08812 FvH4_7g08990 FvH4_7g08991
rosa_chinensis RchiOBHm_Chr0c11g0499321 RchiOBHm_Chr0c11g0499331 RchiOBHm_Chr0c11g0499401 RchiOBHm_Chr0c11g0499411 RchiOBHm_Chr0c11g0499461 RchiOBHm_Chr0c11g0499471 RchiOBHm_Chr0c11g0499481 RchiOBHm_Chr0c11g0499501 RchiOBHm_Chr0c11g0499511 RchiOBHm_Chr0c11g0499551 RchiOBHm_Chr1g0342791 RchiOBHm_Chr1g0342991 RchiOBHm_Chr4g0419921 RchiOBHm_Chr4g0420021 RchiOBHm_Chr7g0193131 RchiOBHm_Chr7g0218371 RchiOBHm_Chr7g0218381 RchiOBHm_Chr7g0235811 RchiOBHm_Chr7g0235841 RchiOBHm_Chr7g0235851 RchiOBHm_Chr7g0235871 RchiOBHm_Chr7g0235881 RchiOBHm_Chr7g0235901 RchiOBHm_Chr7g0235921 RchiOBHm_Chr7g0235931 RchiOBHm_Chr7g0235941 RchiOBHm_Chr7g0235971 RchiOBHm_Chr7g0235981 RchiOBHm_Chr7g0235991 RchiOBHm_Chr7g0236021 RchiOBHm_Chr7g0236031 RchiOBHm_Chr7g0236041 RchiOBHm_Chr7g0236051 RchiOBHm_Chr7g0236071 RchiOBHm_Chr7g0236101 RchiOBHm_Chr7g0236111 RchiOBHm_Chr7g0236191 RchiOBHm_Chr7g0236201 RchiOBHm_Chr7g0236231 RchiOBHm_Chr7g0236241 RchiOBHm_Chr7g0236331 RchiOBHm_Chr7g0236591 RchiOBHm_Chr7g0236621 RchiOBHm_Chr7g0236631
rosa_laevigata RLG00000000990 RLG00000000992 RLG00000001071 RLG00000001073 RLG00000001074 RLG00000001075 RLG00000001083 RLG00000001096 RLG00000001097 RLG00000001099 RLG00000001106 RLG00000001108 RLG00000001110 RLG00000001112 RLG00000001119 RLG00000001120 RLG00000001123 RLG00000001128 RLG00000001129 RLG00000001130 RLG00000001131 RLG00000001132 RLG00000001133 RLG00000001136 RLG00000001255 RLG00000001929 RLG00000001930 RLG00000001932 RLG00000001934 RLG00000001935 RLG00000001940 RLG00000001942 RLG00000002624 RLG00000004293 RLG00000004294 RLG00000007792 RLG00000030009 RLG00000030011 RLG00000030012 RLG00000030014
rosa_multiflora Rmu_co8114088.1_g000001 Rmu_co8163812.1_g000001 Rmu_co8175782.1_g000001 Rmu_co8217500.1_g000001 Rmu_co8230787.1_g000001 Rmu_co8372547.1_g000001 Rmu_co8409897.1_g000001 Rmu_sc0000755.1_g000001 Rmu_sc0000755.1_g000003 Rmu_sc0002449.1_g000024 Rmu_sc0002536.1_g000007 Rmu_sc0002536.1_g000008 Rmu_sc0002536.1_g000013 Rmu_sc0002536.1_g000014 Rmu_sc0002921.1_g000004 Rmu_sc0002921.1_g000005 Rmu_sc0002921.1_g000007 Rmu_sc0003339.1_g000004 Rmu_sc0003461.1_g000006 Rmu_sc0005998.1_g000001 Rmu_sc0008183.1_g000003 Rmu_sc0008183.1_g000007 Rmu_sc0008183.1_g000009 Rmu_sc0008183.1_g000010 Rmu_sc0008183.1_g000011 Rmu_sc0008183.1_g000020 Rmu_sc0008183.1_g000022 Rmu_sc0008183.1_g000026 Rmu_sc0008940.1_g000007 Rmu_sc0008941.1_g000001 Rmu_sc0008941.1_g000002 Rmu_sc0008941.1_g000004 Rmu_sc0008941.1_g000005 Rmu_sc0008941.1_g000006 Rmu_sc0011173.1_g000001 Rmu_sc0011173.1_g000002 Rmu_sc0011173.1_g000008 Rmu_sc0011173.1_g000009 Rmu_sc0011173.1_g000013 Rmu_sc0011290.1_g000002 Rmu_sc0012908.1_g000002 Rmu_sc0013209.1_g000003 Rmu_sc0025395.1_g000001 Rmu_sc0025941.1_g000001 Rmu_sc0029089.1_g000001 Rmu_sc0036318.1_g000001 Rmu_sc0040213.1_g000001 Rmu_ssc0000092.1_g000038
rosa_roxburghii Rroxscaffold_3G00225010 Rroxscaffold_3G00225220 Rroxscaffold_3G00225300 Rroxscaffold_3G00225310 Rroxscaffold_3G00225390 Rroxscaffold_3G00225400 Rroxscaffold_3G00225470 Rroxscaffold_3G00225500 Rroxscaffold_3G00225570 Rroxscaffold_3G00225580 Rroxscaffold_3G00225600 Rroxscaffold_3G00225610 Rroxscaffold_3G00262350 Rroxscaffold_4G00313250
rosa_rugosa Rorug01G0145100.1 Rorug01G0145200.1 Rorug01G0145300.1 Rorug01G0145500.1 Rorug04G0161500 Rorug04G0161600 Rorug07G0007900 Rorug07G0008000 Rorug07G0008000 Rorug07G0273500 Rorug07G0273900 Rorug07G0274000 Rorug07G0274000 Rorug07G0274200.1 Rorug07G0274300 Rorug07G0274400 Rorug07G0293100 Rorug07G0293200 Rorug07G0293300 Rorug07G0293400 Rorug07G0293700 Rorug07G0293800 Rorug07G0294000 Rorug07G0294200 Rorug07G0294700 Rorug07G0294700 Rorug07G0295000 Rorug07G0295100 Rorug07G0295200 Rorug07G0295400 Rorug07G0295400 Rorug07G0296100 Rorug07G0297900 Rorug07G0297900 Rorug07G0298000 Rorug07G0298100
rosa_samantha Rh1DG165000 Rh1DG165100 Rh1DG165200 Rh1DG165600 Rh1DG165700 Rh1DG165800 Rh4DG221500 Rh7DG134800 Rh7DG134900 Rh7DG436900 Rh7DG437100 Rh7DG437300 Rh7DG437400 Rh7DG437500 Rh7DG437700 Rh7DG437800 Rh7DG438200 Rh7DG439200 Rh7DG439300 Rh7DG440300
rosa_wichuraiana Rw0G019880 Rw0G020650 Rw0G020670 Rw1G013620 Rw1G013630 Rw4G019180 Rw7G011360 Rw7G011370 Rw7G037220 Rw7G037240 Rw7G037260 Rw7G037270 Rw7G037280 Rw7G037300 Rw7G037380 Rw7G037390 Rw7G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccI GTMKAC 2 cut(s) 249, 276
AciI CCGC 2 cut(s) 280, 294
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 191, 478
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 1 cut(s) 103
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 491
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AleI CACNNNNGTG 1 cut(s) 449
AluBI AGCT 2 cut(s) 169, 427
AluI AGCT 2 cut(s) 169, 427
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 20, 307
AoxI GGCC 3 cut(s) 72, 234, 489
ApeKI GCWGC 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 91, 234
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 87, 208
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 250
BceAI ACGGC 2 cut(s) 197, 456
BcgI CGANNNNNNTGC 2 cut(s) 336, 370
BciT130I CCWGG 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 20, 307
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BisI GCNGC 2 cut(s) 264, 294
BlsI GCNGC 2 cut(s) 265, 295
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 493
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 91, 234
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 1 cut(s) 448
BoxI GACNNNNGTC 1 cut(s) 12
BpiI GAAGAC 2 cut(s) 87, 208
Bpu10I CCTNAGC 1 cut(s) 180
BpuEI CTTGAG 2 cut(s) 26, 254
BsaJI CCNNGG 2 cut(s) 258, 492
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 478
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 258, 492
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 191, 478
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 177, 194
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 250
BshFI GGCC 3 cut(s) 74, 236, 491
BsiHKAI GWGCWC 1 cut(s) 171
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 191, 478
BsmAI GTCTC 2 cut(s) 20, 307
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 3 cut(s) 74, 236, 491
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 2 cut(s) 280, 294
BspANI GGCC 3 cut(s) 74, 236, 491
BspCNI CTCAG 2 cut(s) 178, 193
BsrDI GCAATG 1 cut(s) 462
BssECI CCNNGG 2 cut(s) 258, 492
BssMI GATC 1 cut(s) 120
BssNAI GTATAC 1 cut(s) 277
Bst1107I GTATAC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 2 cut(s) 86, 451
BstC8I GCNNGC 2 cut(s) 298, 469
BstDEI CTNAG 2 cut(s) 180, 186
BstF5I GGATG 1 cut(s) 426
BstKTI GATC 1 cut(s) 123
BstMAI GTCTC 2 cut(s) 20, 307
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 175, 488
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 53
BstPAI GACNNNNGTC 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 491
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 2 cut(s) 87, 208
BstZ17I GTATAC 1 cut(s) 277
BsuRI GGCC 3 cut(s) 74, 236, 491
BtsCI GGATG 1 cut(s) 426
BtsIMutI CAGTG 2 cut(s) 201, 447
Cac8I GCNNGC 2 cut(s) 298, 469
Cfr13I GGNCC 2 cut(s) 91, 234
CseI GACGC 1 cut(s) 289
Csp6I GTAC 1 cut(s) 394
CviAII CATG 1 cut(s) 50
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 2 cut(s) 180, 186
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 491
EcoT38I GRGCYC 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 1 cut(s) 53
FaiI YATR 7 cut(s) 51, 246, 277, 322, 329, 384, 514
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 140
FatI CATG 1 cut(s) 49
FblI GTMKAC 2 cut(s) 249, 276
Fnu4HI GCNGC 2 cut(s) 264, 294
FokI GGATG 1 cut(s) 433
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 264, 294
FspBI CTAG 1 cut(s) 12
GluI GCNGC 2 cut(s) 264, 294
HaeIII GGCC 3 cut(s) 74, 236, 491
HgaI GACGC 1 cut(s) 289
Hin1II CATG 1 cut(s) 53
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 4 cut(s) 116, 134, 188, 476
Hpy166II GTNNAC 2 cut(s) 250, 277
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 2 cut(s) 250, 277
HpyAV CCTTC 2 cut(s) 52, 481
HpyCH4III ACNGT 2 cut(s) 86, 451
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 488
HpyF3I CTNAG 2 cut(s) 180, 186
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 1 cut(s) 53
Kzo9I GATC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 7 cut(s) 109, 198, 296, 310, 478, 481, 505
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 1 cut(s) 448
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 207
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 3 cut(s) 18, 92, 208
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 1 cut(s) 22
MlyI GAGTC 4 cut(s) 110, 128, 197, 485
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 7 cut(s) 50, 61, 64, 82, 189, 253, 349
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 2 cut(s) 141, 174
MslI CAYNNNNRTG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 493
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 1 cut(s) 493
MwoI GCNNNNNNNGC 2 cut(s) 175, 488
NdeII GATC 1 cut(s) 120
NlaIII CATG 1 cut(s) 53
NspI RCATGY 1 cut(s) 53
OliI CACNNNNGTG 1 cut(s) 449
PciI ACATGT 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PctI GAATGC 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 265, 295
PleI GAGTC 4 cut(s) 110, 128, 196, 484
PpsI GAGTC 4 cut(s) 110, 128, 196, 484
PpuMI RGGWCCY 1 cut(s) 91
PscI ACATGT 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 491
PspPI GGNCC 2 cut(s) 91, 234
PspPPI RGGWCCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 449
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 141, 174
SatI GCNGC 2 cut(s) 264, 294
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 2 cut(s) 91, 234
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 4 cut(s) 110, 128, 197, 485
ScrFI CCNGG 1 cut(s) 493
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 1 cut(s) 448
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 41, 269
SmoI CTYRAG 2 cut(s) 41, 269
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 1 cut(s) 22
SsiI CCGC 2 cut(s) 280, 294
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 1 cut(s) 491
TaaI ACNGT 2 cut(s) 86, 451
TaiI ACGT 1 cut(s) 210
TaqI TCGA 3 cut(s) 66, 119, 249
TaqII GACCGA 1 cut(s) 272
TasI AATT 1 cut(s) 22
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 2 cut(s) 141, 174
Tru9I TTAA 2 cut(s) 141, 174
TscAI CASTG 2 cut(s) 208, 454
TseI GCWGC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 71, 171, 437
TspRI CASTG 2 cut(s) 208, 454
VpaK11BI GGWCC 1 cut(s) 91
XceI RCATGY 1 cut(s) 53
XmiI GTMKAC 2 cut(s) 249, 276
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.