FvH4_5g35330

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000101
Physical Location & Seq
Reverse (-)
25878227 .. 25880097
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35330.t1

Sequence Viewer

Length: 930 bp
ATGCAAAAGCGATTCAAATGTTCAGTTCTATGGCAGATTCCGGCTTCCTTGATGAAGCGAAAGAGCTTTTTAAGCCACTCTCTGGTGAGGTCACTTTGCCTCAAGTGGCTGTCTTCACCTTGGTCGTTTCAAAACGCCCGCGCTGGCATGGCCATGGATGCTCACAAGGTCTACCAGCAGATGATAGCCTCTGGTGTCACCCCCACCGCCTACATTTACACTGCACTCATTAGTGGATTTGCAAAAGACTTGTCCATTTTGGATTCTGTCGGGTATGCCAAGAAGTATTTTCTTGAAATGTTGGACAGGGGGATGAAGCCCCATTATAAACCTTACATGGATCTTATAAATGCCATTGCCTGCGAAGAGCCAGAGGAGGAGGTTAATAAGTTCCTTGAGCAGATAAAGGCCAAGGGGCTCAGCCTTGACAACTATAGTTTTCCATACAAAGAAGGCCACCTGACAAAACAGATGGAGGCAATCTGCATGTCTACTGATCTTGTCGACAACAATACTGTTGTGAAAAAATATCTTGAGAAGTTGTTGGCTTCACCTTGGGAAATCATTGAACTTTCATTGAGTATGCACTGTGCTTTGCTAGAAGATGGAAACGTCAATGAAGCTACAAAGGTCTGTAACTCAATTCAGCACACAGTCACAGAGCCCTCGGTCATACTCCACACCTTGGTCATTGAATCCTACCTCAAGTTCGGCAAGGTCAAGGGTGCTCTGAAGGCCTACCGGGGAATGTTAGCTGCGGGTGTTGCCCCCAACTCCTACACTTACACAGTTTTAATCAAGGGGCTCACTGCTGATCCCCTCTTCTCTGGAGATGCCAAAAAGTGCTTGCTTGAGATGATGGAAAAAGGGGATGAGGCCCAATGCTGCTACCTACACTGTTGTGATAGAGAGCTTTGCAAAGCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

310

Amino Acids

34.69

Weight (kDa)

8.16

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 222 - 269 4.5e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28420
fragaria_vesca FvH4_3g36751 FvH4_4g16590 FvH4_4g16590 FvH4_4g16590 FvH4_5g05670 FvH4_5g05700 FvH4_5g31002 FvH4_5g31070 FvH4_5g31080 FvH4_5g31080 FvH4_5g31080 FvH4_5g31150 FvH4_5g32070 FvH4_5g32080 FvH4_5g34900 FvH4_5g34930 FvH4_5g34930 FvH4_5g34950 FvH4_5g35000 FvH4_5g35000 FvH4_5g35000 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35060 FvH4_5g35070 FvH4_5g35071 FvH4_5g35080 FvH4_5g35253 FvH4_5g35254 FvH4_5g35300 FvH4_5g35300 FvH4_5g35320 FvH4_5g35320 FvH4_5g35320 FvH4_5g35322 FvH4_5g35330 FvH4_6g08812 FvH4_7g08990 FvH4_7g08991
rosa_chinensis RchiOBHm_Chr0c11g0499321 RchiOBHm_Chr0c11g0499331 RchiOBHm_Chr0c11g0499401 RchiOBHm_Chr0c11g0499411 RchiOBHm_Chr0c11g0499461 RchiOBHm_Chr0c11g0499471 RchiOBHm_Chr0c11g0499481 RchiOBHm_Chr0c11g0499501 RchiOBHm_Chr0c11g0499511 RchiOBHm_Chr0c11g0499551 RchiOBHm_Chr1g0342791 RchiOBHm_Chr1g0342991 RchiOBHm_Chr4g0419921 RchiOBHm_Chr4g0420021 RchiOBHm_Chr7g0193131 RchiOBHm_Chr7g0218371 RchiOBHm_Chr7g0218381 RchiOBHm_Chr7g0235811 RchiOBHm_Chr7g0235841 RchiOBHm_Chr7g0235851 RchiOBHm_Chr7g0235871 RchiOBHm_Chr7g0235881 RchiOBHm_Chr7g0235901 RchiOBHm_Chr7g0235921 RchiOBHm_Chr7g0235931 RchiOBHm_Chr7g0235941 RchiOBHm_Chr7g0235971 RchiOBHm_Chr7g0235981 RchiOBHm_Chr7g0235991 RchiOBHm_Chr7g0236021 RchiOBHm_Chr7g0236031 RchiOBHm_Chr7g0236041 RchiOBHm_Chr7g0236051 RchiOBHm_Chr7g0236071 RchiOBHm_Chr7g0236101 RchiOBHm_Chr7g0236111 RchiOBHm_Chr7g0236191 RchiOBHm_Chr7g0236201 RchiOBHm_Chr7g0236231 RchiOBHm_Chr7g0236241 RchiOBHm_Chr7g0236331 RchiOBHm_Chr7g0236591 RchiOBHm_Chr7g0236621 RchiOBHm_Chr7g0236631
rosa_laevigata RLG00000000990 RLG00000000992 RLG00000001071 RLG00000001073 RLG00000001074 RLG00000001075 RLG00000001083 RLG00000001096 RLG00000001097 RLG00000001099 RLG00000001106 RLG00000001108 RLG00000001110 RLG00000001112 RLG00000001119 RLG00000001120 RLG00000001123 RLG00000001128 RLG00000001129 RLG00000001130 RLG00000001131 RLG00000001132 RLG00000001133 RLG00000001136 RLG00000001255 RLG00000001929 RLG00000001930 RLG00000001932 RLG00000001934 RLG00000001935 RLG00000001940 RLG00000001942 RLG00000002624 RLG00000004293 RLG00000004294 RLG00000007792 RLG00000030009 RLG00000030011 RLG00000030012 RLG00000030014
rosa_multiflora Rmu_co8114088.1_g000001 Rmu_co8163812.1_g000001 Rmu_co8175782.1_g000001 Rmu_co8217500.1_g000001 Rmu_co8230787.1_g000001 Rmu_co8372547.1_g000001 Rmu_co8409897.1_g000001 Rmu_sc0000755.1_g000001 Rmu_sc0000755.1_g000003 Rmu_sc0002449.1_g000024 Rmu_sc0002536.1_g000007 Rmu_sc0002536.1_g000008 Rmu_sc0002536.1_g000013 Rmu_sc0002536.1_g000014 Rmu_sc0002921.1_g000004 Rmu_sc0002921.1_g000005 Rmu_sc0002921.1_g000007 Rmu_sc0003339.1_g000004 Rmu_sc0003461.1_g000006 Rmu_sc0005998.1_g000001 Rmu_sc0008183.1_g000003 Rmu_sc0008183.1_g000007 Rmu_sc0008183.1_g000009 Rmu_sc0008183.1_g000010 Rmu_sc0008183.1_g000011 Rmu_sc0008183.1_g000020 Rmu_sc0008183.1_g000022 Rmu_sc0008183.1_g000026 Rmu_sc0008940.1_g000007 Rmu_sc0008941.1_g000001 Rmu_sc0008941.1_g000002 Rmu_sc0008941.1_g000004 Rmu_sc0008941.1_g000005 Rmu_sc0008941.1_g000006 Rmu_sc0011173.1_g000001 Rmu_sc0011173.1_g000002 Rmu_sc0011173.1_g000008 Rmu_sc0011173.1_g000009 Rmu_sc0011173.1_g000013 Rmu_sc0011290.1_g000002 Rmu_sc0012908.1_g000002 Rmu_sc0013209.1_g000003 Rmu_sc0025395.1_g000001 Rmu_sc0025941.1_g000001 Rmu_sc0029089.1_g000001 Rmu_sc0036318.1_g000001 Rmu_sc0040213.1_g000001 Rmu_ssc0000092.1_g000038
rosa_roxburghii Rroxscaffold_3G00225010 Rroxscaffold_3G00225220 Rroxscaffold_3G00225300 Rroxscaffold_3G00225310 Rroxscaffold_3G00225390 Rroxscaffold_3G00225400 Rroxscaffold_3G00225470 Rroxscaffold_3G00225500 Rroxscaffold_3G00225570 Rroxscaffold_3G00225580 Rroxscaffold_3G00225600 Rroxscaffold_3G00225610 Rroxscaffold_3G00262350 Rroxscaffold_4G00313250
rosa_rugosa Rorug01G0145100.1 Rorug01G0145200.1 Rorug01G0145300.1 Rorug01G0145500.1 Rorug04G0161500 Rorug04G0161600 Rorug07G0007900 Rorug07G0008000 Rorug07G0008000 Rorug07G0273500 Rorug07G0273900 Rorug07G0274000 Rorug07G0274000 Rorug07G0274200.1 Rorug07G0274300 Rorug07G0274400 Rorug07G0293100 Rorug07G0293200 Rorug07G0293300 Rorug07G0293400 Rorug07G0293700 Rorug07G0293800 Rorug07G0294000 Rorug07G0294200 Rorug07G0294700 Rorug07G0294700 Rorug07G0295000 Rorug07G0295100 Rorug07G0295200 Rorug07G0295400 Rorug07G0295400 Rorug07G0296100 Rorug07G0297900 Rorug07G0297900 Rorug07G0298000 Rorug07G0298100
rosa_samantha Rh1DG165000 Rh1DG165100 Rh1DG165200 Rh1DG165600 Rh1DG165700 Rh1DG165800 Rh4DG221500 Rh7DG134800 Rh7DG134900 Rh7DG436900 Rh7DG437100 Rh7DG437300 Rh7DG437400 Rh7DG437500 Rh7DG437700 Rh7DG437800 Rh7DG438200 Rh7DG439200 Rh7DG439300 Rh7DG440300
rosa_wichuraiana Rw0G019880 Rw0G020650 Rw0G020670 Rw1G013620 Rw1G013630 Rw4G019180 Rw7G011360 Rw7G011370 Rw7G037220 Rw7G037240 Rw7G037260 Rw7G037270 Rw7G037280 Rw7G037300 Rw7G037380 Rw7G037390 Rw7G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 327, 347
AccB7I CCANNNNNTGG 2 cut(s) 82, 685
AccI GTMKAC 3 cut(s) 171, 491, 504
AccII CGCG 1 cut(s) 141
AciI CCGC 3 cut(s) 139, 207, 758
AclWI GGATC 2 cut(s) 348, 809
AcoI YGGCCR 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 82, 685
AgsI TTSAA 5 cut(s) 16, 131, 296, 569, 695
AleI CACNNNNGTG 1 cut(s) 900
AluBI AGCT 4 cut(s) 66, 623, 755, 913
AluI AGCT 4 cut(s) 66, 623, 755, 913
Alw21I GWGCWC 1 cut(s) 730
AlwI GGATC 2 cut(s) 348, 809
AoxI GGCC 5 cut(s) 150, 408, 454, 735, 876
ApeKI GCWGC 2 cut(s) 755, 885
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 877
AsuC2I CCSGG 1 cut(s) 743
AsuHPI GGTGA 4 cut(s) 97, 108, 190, 543
BalI TGGCCA 1 cut(s) 152
BanII GRGCYC 3 cut(s) 420, 666, 807
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 2 cut(s) 742, 872
BccI CCATC 3 cut(s) 466, 599, 853
BcnI CCSGG 1 cut(s) 743
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 433
BisI GCNGC 2 cut(s) 756, 886
BlpI GCTNAGC 1 cut(s) 419
BlsI GCNGC 2 cut(s) 757, 887
Bme1390I CCNGG 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 877
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 2 cut(s) 148, 823
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 849
Bpu1102I GCTNAGC 1 cut(s) 419
BpuEI CTTGAG 5 cut(s) 86, 416, 554, 689, 872
BpuMI CCSGG 1 cut(s) 743
BsaJI CCNNGG 7 cut(s) 119, 153, 411, 554, 666, 684, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 685
Bse3DI GCAATG 1 cut(s) 354
BseDI CCNNGG 7 cut(s) 119, 153, 411, 554, 666, 684, 742
BseGI GGATG 3 cut(s) 163, 318, 877
BseLI CCNNNNNNNGG 2 cut(s) 82, 685
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 433
BseRI GAGGAG 2 cut(s) 389, 392
BseXI GCAGC 2 cut(s) 742, 872
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 5 cut(s) 152, 410, 456, 737, 878
BsiHKAI GWGCWC 1 cut(s) 730
BsiSI CCGG 2 cut(s) 41, 742
BslI CCNNNNNNNGG 2 cut(s) 82, 685
BsnI GGCC 5 cut(s) 152, 410, 456, 737, 878
Bsp1286I GDGCHC 4 cut(s) 420, 666, 730, 807
Bsp143I GATC 3 cut(s) 340, 496, 814
Bsp1720I GCTNAGC 1 cut(s) 419
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 3 cut(s) 139, 207, 758
BspANI GGCC 5 cut(s) 152, 410, 456, 737, 878
BspCNI CTCAG 1 cut(s) 432
BspFNI CGCG 1 cut(s) 141
BspPI GGATC 2 cut(s) 348, 809
BspQI GCTCTTC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 354
BssECI CCNNGG 7 cut(s) 119, 153, 411, 554, 666, 684, 742
BssMI GATC 3 cut(s) 340, 496, 814
BssT1I CCWWGG 5 cut(s) 119, 153, 411, 554, 684
Bst4CI ACNGT 5 cut(s) 517, 590, 655, 790, 899
Bst6I CTCTTC 2 cut(s) 360, 827
BstC8I GCNNGC 4 cut(s) 139, 145, 361, 848
BstDEI CTNAG 1 cut(s) 419
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 163, 318, 877
BstFNI CGCG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 3 cut(s) 343, 499, 817
BstMBI GATC 3 cut(s) 340, 496, 814
BstMWI GCNNNNNNNGC 5 cut(s) 72, 149, 158, 734, 764
BstNSI RCATGY 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 433
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 2 cut(s) 742, 872
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuRI GGCC 5 cut(s) 152, 410, 456, 737, 878
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 163, 318, 877
BtsI GCAGTG 2 cut(s) 219, 807
BtsIMutI CAGTG 4 cut(s) 219, 586, 807, 895
Cac8I GCNNGC 4 cut(s) 139, 145, 361, 848
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 877
CviAII CATG 4 cut(s) 148, 154, 337, 487
DdeI CTNAG 1 cut(s) 419
DpnI GATC 3 cut(s) 342, 498, 816
DpnII GATC 3 cut(s) 340, 496, 814
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 2 cut(s) 360, 827
EarI CTCTTC 2 cut(s) 360, 827
Eco130I CCWWGG 5 cut(s) 119, 153, 411, 554, 684
Eco147I AGGCCT 1 cut(s) 737
Eco24I GRGCYC 3 cut(s) 420, 666, 807
Eco57I CTGAAG 1 cut(s) 752
EcoT14I CCWWGG 5 cut(s) 119, 153, 411, 554, 684
EcoT38I GRGCYC 3 cut(s) 420, 666, 807
ErhI CCWWGG 5 cut(s) 119, 153, 411, 554, 684
FaeI CATG 4 cut(s) 151, 157, 340, 490
FatI CATG 4 cut(s) 147, 153, 336, 486
FauI CCCGC 2 cut(s) 146, 751
FblI GTMKAC 3 cut(s) 171, 491, 504
Fnu4HI GCNGC 2 cut(s) 756, 886
FokI GGATG 3 cut(s) 170, 325, 884
FriOI GRGCYC 3 cut(s) 420, 666, 807
Fsp4HI GCNGC 2 cut(s) 756, 886
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 142
GluI GCNGC 2 cut(s) 756, 886
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 5 cut(s) 152, 410, 456, 737, 878
HapII CCGG 2 cut(s) 41, 742
HhaI GCGC 1 cut(s) 143
Hin1II CATG 4 cut(s) 151, 157, 340, 490
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 12, 37, 263, 695
HpaII CCGG 2 cut(s) 41, 742
HphI GGTGA 4 cut(s) 97, 108, 190, 543
Hpy166II GTNNAC 3 cut(s) 172, 492, 505
Hpy188I TCNGA 1 cut(s) 732
Hpy188III TCNNGA 3 cut(s) 293, 533, 828
Hpy8I GTNNAC 3 cut(s) 172, 492, 505
HpyAV CCTTC 2 cut(s) 446, 727
HpyCH4III ACNGT 5 cut(s) 517, 590, 655, 790, 899
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 6 cut(s) 4, 224, 242, 486, 586, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 149, 158, 734, 764
HpyF3I CTNAG 1 cut(s) 419
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 4 cut(s) 151, 157, 340, 490
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 3 cut(s) 340, 496, 814
LguI GCTCTTC 1 cut(s) 360
Lsp1109I GCAGC 2 cut(s) 742, 872
LweI GCATC 2 cut(s) 148, 823
MaeI CTAG 1 cut(s) 599
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 4 cut(s) 90, 196, 635, 655
MalI GATC 3 cut(s) 342, 498, 816
MboI GATC 3 cut(s) 340, 496, 814
MboII GAAGA 4 cut(s) 105, 377, 614, 814
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 4 cut(s) 420, 666, 730, 807
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 642
MluNI TGGCCA 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
MseI TTAA 3 cut(s) 71, 384, 794
MslI CAYNNNNRTG 2 cut(s) 152, 900
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 2 cut(s) 41, 742
MspR9I CCNGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 5 cut(s) 72, 149, 158, 734, 764
NciI CCSGG 1 cut(s) 743
NcoI CCATGG 1 cut(s) 153
NdeII GATC 3 cut(s) 340, 496, 814
NlaIII CATG 4 cut(s) 151, 157, 340, 490
NmuCI GTSAC 3 cut(s) 90, 196, 655
NspI RCATGY 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 900
PceI AGGCCT 1 cut(s) 737
PciSI GCTCTTC 1 cut(s) 360
PfeI GAWTC 4 cut(s) 12, 37, 263, 695
PflMI CCANNNNNTGG 2 cut(s) 82, 685
PkrI GCNGC 2 cut(s) 757, 887
PsiI TTATAA 2 cut(s) 327, 347
PspPI GGNCC 1 cut(s) 877
PsuI RGATCY 1 cut(s) 340
RseI CAYNNNNRTG 2 cut(s) 152, 900
SalI GTCGAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 3 cut(s) 71, 384, 794
SatI GCNGC 2 cut(s) 756, 886
Sau3AI GATC 3 cut(s) 340, 496, 814
Sau96I GGNCC 1 cut(s) 877
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 4 cut(s) 420, 666, 730, 807
SfaNI GCATC 2 cut(s) 148, 823
SfcI CTRYAG 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 152, 900
SmlI CTYRAG 5 cut(s) 101, 395, 533, 704, 851
SmoI CTYRAG 5 cut(s) 101, 395, 533, 704, 851
Sse9I AATT 1 cut(s) 642
SseBI AGGCCT 1 cut(s) 737
SsiI CCGC 3 cut(s) 139, 207, 758
SspMI CTAG 1 cut(s) 599
StuI AGGCCT 1 cut(s) 737
StyD4I CCNGG 1 cut(s) 741
StyI CCWWGG 5 cut(s) 119, 153, 411, 554, 684
TaaI ACNGT 5 cut(s) 517, 590, 655, 790, 899
TaiI ACGT 1 cut(s) 615
TaqI TCGA 1 cut(s) 504
TaqII GACCGA 1 cut(s) 658
TasI AATT 1 cut(s) 642
TfiI GAWTC 4 cut(s) 12, 37, 263, 695
Tru1I TTAA 3 cut(s) 71, 384, 794
Tru9I TTAA 3 cut(s) 71, 384, 794
TscAI CASTG 4 cut(s) 226, 593, 814, 902
TseFI GTSAC 3 cut(s) 90, 196, 655
TseI GCWGC 2 cut(s) 755, 885
Tsp45I GTSAC 3 cut(s) 90, 196, 655
TspDTI ATGAA 4 cut(s) 68, 329, 564, 633
TspRI CASTG 4 cut(s) 226, 593, 814, 902
Van91I CCANNNNNTGG 2 cut(s) 82, 685
XceI RCATGY 1 cut(s) 490
XmiI GTMKAC 3 cut(s) 171, 491, 504
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.