Rh7DG436900

hydrolase activity, acting on ester bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000101
Physical Location & Seq
Forward (+)
62260460 .. 62272622
12163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG436900.1

Sequence Viewer

Length: 2235 bp
ATGGCGGAGGCTAGTTCCCCAAATTACGATGACCTCAAGAACCATGTAATGAAGGTTTTTGAGGATGCGACCAAAGACTGTGAGGACAAGGACATGAAAAAAGTATTCGATTCGATTGGGGACTACGACTCCTTTAACACCTATGCGATTGAAATGTTCGGTGCTATGGAAAACTTGGGCCTCGGTGACGAAGCCAGAGAGCTGTTCAGGCCTCTCTCTGACCGAGCCGTGCTGCGGGACGTGGCTGTCCACACTGTGGTCATTCGGGCATACGCTGTTGCTGGCAGGACCAAGGCTGTTCTCAAGGCCTACCAGCACATGATAGCCACTGGTATCGCTCCCTCATCCCTCACTTACACCATACTCATCAATGCACTTGTAGTAGACTATTCTGATTACAATTTTGTTGGCTATGTGAAGAACTATATTCTAGAAATGTTGGATAAGGGGATGAAGCCTCATCCTATATGCTATTTGGGTCTGTTTGGAGTCCTTGGCTGCCGGGAGTCGGTGGAGAAGGCCACTGAGTTCCTTGTGCTGATAAATGCCAAGGGGTTGAGCCCCGAGTCCAATTATTTTAACGTTGAAGAAGGCCACTTCAAAAATGCGATGAAGGCAATCAAGGTGTACACTAATGTCAGCAATATAACAACTGACAAGGATGCGCAAAAGGTGTTCCGGAAGTGGCGCACCAACGCCCCTGGCCTCAAGAAAGAGTTAGTGAAGATGATCAATGCTCTGGTGAAGGATGGCAACCTAGATGAATCCATGGAGATGTATAGGGATATCATTGAGACAGGAAGAGAACCTTTTGTCCTACTCCACACCTGTGTTATTAAAGCCTATCTCAAGTCTGGCAAGACCAAGAGTGCTTTTGAGCTGTCCCAGGGTATGTTAGCTGCAGGTGTTTCCCCCAACTCATACACTTACACAGTTTTAATCAAGGGACTCGCTGCTGACCCCAACTTCTTTGGAGAAGCCAAGAAGTGCTTGCTTGAAATGATGGACAGGGGGAAACGGCCCAATGCTGCTACCTACACTGCTCTGATAGAGCTATTTGCAAGGCAGGAGGACAAGGCAGCTGAGGAGGAGGGTAAAGAGTTTGTGGAGAAAACCTTTAGGATTGTGGCGTTTGAAGCAGTAGCACTCAATTTGGAGTGTGAGGAAGCAGTAGCACTCAGTTTGGAGTGTGGGGAAGCAGTAGCCTCTTCTAAAAATGCTGAAGCTGGGGACCGACCAGGTGAAATCAACAGAATGTCGTCGCCGTTCGTAGGGGTTTTCGCCTTTGGAGATTCGGTTCTTGACACAGGCAACAACAATGACCTCCCTACTGTAGCCAGATGCAATTTCCCACCCTATGGAAGAGATTTTATAGGAGGCATCCCTACGGGAAGATTTAGCAATGGGAAAGTTTACTCCGACATTATAGTGGAAGCCTTGGGTATCAAGCATCTCTTGCCAGCATACCTTGACCCAAACCTACGCTCTGAAGATCTCCCAACTGGCGTATGCTTTGCTTCTGGCAATTCCGGTTATGATCCTCTAACACCATCTCTAGTGGGAGTTCTATCTTTGTCAGATCAGCTAGAACTCTTCAAAGACTATAGAGTCAAACTAACAGGATTTGTTGGAGAAGAGAAGGCAAAATCCATCATAGCCAACAGTCTATTTCTAGTTTCGTCAGGCAACAATGATATTCTTATTACATATTCTCTCTATATCCAACAATTTCAGTATGATTTTCACTCATATGCTACTCTTTTGGTTAGCTTCGCTTCTACATTTTTAAAGGATTTATATAGCCTAGGGGCACGACGAATTGCTATCGTAAGTGCATTCCCACTGGGATGCGCACCATTGGAAAGAAATGGAGAAGGATTACTAGGAGAGTGTTTAGAGTTGCAAAACCAAAGAGCAAAGATGTTCAACTCTCTATTATCATCCGAATTAGATACCATCAACAGGGACTTCCCTGACGCGAAGTTAATTTTTCTTGATGTCTACCACCCTTTTCTTGAACTCAATCAACACCCTGAAAACTCAGGATTCGAAGTCTTGAGGATTGGGTGTTGTGGTACTGGAACATTTGAAGTGACATCGTTTTTATGTAACCCATGGAATCCATTCACATGTTCAAATGCATCAAATTATCTATACTGGGATGCAATTCATCCGAGCGAACGAGCTCAAAGAATCGTCGCTTCTCAAACTCTCAAATTCATCACGAGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

744

Amino Acids

82.43

Weight (kDa)

5.24

Isoelectric Point (pI)

27.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 80 - 126 5.2e-08 PPR repeat family
PPR_2 PF13041 276 - 318 8.8e-12 PPR repeat family
TPR_24 PF23276 276 - 359 1.2e-06 Fungal tetratrico peptide repeats
Lipase_GDSL PF00657 426 - 735 6e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28420
fragaria_vesca FvH4_3g36751 FvH4_4g16590 FvH4_4g16590 FvH4_4g16590 FvH4_5g05670 FvH4_5g05700 FvH4_5g31002 FvH4_5g31070 FvH4_5g31080 FvH4_5g31080 FvH4_5g31080 FvH4_5g31150 FvH4_5g32070 FvH4_5g32080 FvH4_5g34900 FvH4_5g34930 FvH4_5g34930 FvH4_5g34950 FvH4_5g35000 FvH4_5g35000 FvH4_5g35000 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35060 FvH4_5g35070 FvH4_5g35071 FvH4_5g35080 FvH4_5g35253 FvH4_5g35254 FvH4_5g35300 FvH4_5g35300 FvH4_5g35320 FvH4_5g35320 FvH4_5g35320 FvH4_5g35322 FvH4_5g35330 FvH4_6g08812 FvH4_7g08990 FvH4_7g08991
rosa_chinensis RchiOBHm_Chr0c11g0499321 RchiOBHm_Chr0c11g0499331 RchiOBHm_Chr0c11g0499401 RchiOBHm_Chr0c11g0499411 RchiOBHm_Chr0c11g0499461 RchiOBHm_Chr0c11g0499471 RchiOBHm_Chr0c11g0499481 RchiOBHm_Chr0c11g0499501 RchiOBHm_Chr0c11g0499511 RchiOBHm_Chr0c11g0499551 RchiOBHm_Chr1g0342791 RchiOBHm_Chr1g0342991 RchiOBHm_Chr4g0419921 RchiOBHm_Chr4g0420021 RchiOBHm_Chr7g0193131 RchiOBHm_Chr7g0218371 RchiOBHm_Chr7g0218381 RchiOBHm_Chr7g0235811 RchiOBHm_Chr7g0235841 RchiOBHm_Chr7g0235851 RchiOBHm_Chr7g0235871 RchiOBHm_Chr7g0235881 RchiOBHm_Chr7g0235901 RchiOBHm_Chr7g0235921 RchiOBHm_Chr7g0235931 RchiOBHm_Chr7g0235941 RchiOBHm_Chr7g0235971 RchiOBHm_Chr7g0235981 RchiOBHm_Chr7g0235991 RchiOBHm_Chr7g0236021 RchiOBHm_Chr7g0236031 RchiOBHm_Chr7g0236041 RchiOBHm_Chr7g0236051 RchiOBHm_Chr7g0236071 RchiOBHm_Chr7g0236101 RchiOBHm_Chr7g0236111 RchiOBHm_Chr7g0236191 RchiOBHm_Chr7g0236201 RchiOBHm_Chr7g0236231 RchiOBHm_Chr7g0236241 RchiOBHm_Chr7g0236331 RchiOBHm_Chr7g0236591 RchiOBHm_Chr7g0236621 RchiOBHm_Chr7g0236631
rosa_laevigata RLG00000000990 RLG00000000992 RLG00000001071 RLG00000001073 RLG00000001074 RLG00000001075 RLG00000001083 RLG00000001096 RLG00000001097 RLG00000001099 RLG00000001106 RLG00000001108 RLG00000001110 RLG00000001112 RLG00000001119 RLG00000001120 RLG00000001123 RLG00000001128 RLG00000001129 RLG00000001130 RLG00000001131 RLG00000001132 RLG00000001133 RLG00000001136 RLG00000001255 RLG00000001929 RLG00000001930 RLG00000001932 RLG00000001934 RLG00000001935 RLG00000001940 RLG00000001942 RLG00000002624 RLG00000004293 RLG00000004294 RLG00000007792 RLG00000030009 RLG00000030011 RLG00000030012 RLG00000030014
rosa_multiflora Rmu_co8114088.1_g000001 Rmu_co8163812.1_g000001 Rmu_co8175782.1_g000001 Rmu_co8217500.1_g000001 Rmu_co8230787.1_g000001 Rmu_co8372547.1_g000001 Rmu_co8409897.1_g000001 Rmu_sc0000755.1_g000001 Rmu_sc0000755.1_g000003 Rmu_sc0002449.1_g000024 Rmu_sc0002536.1_g000007 Rmu_sc0002536.1_g000008 Rmu_sc0002536.1_g000013 Rmu_sc0002536.1_g000014 Rmu_sc0002921.1_g000004 Rmu_sc0002921.1_g000005 Rmu_sc0002921.1_g000007 Rmu_sc0003339.1_g000004 Rmu_sc0003461.1_g000006 Rmu_sc0005998.1_g000001 Rmu_sc0008183.1_g000003 Rmu_sc0008183.1_g000007 Rmu_sc0008183.1_g000009 Rmu_sc0008183.1_g000010 Rmu_sc0008183.1_g000011 Rmu_sc0008183.1_g000020 Rmu_sc0008183.1_g000022 Rmu_sc0008183.1_g000026 Rmu_sc0008940.1_g000007 Rmu_sc0008941.1_g000001 Rmu_sc0008941.1_g000002 Rmu_sc0008941.1_g000004 Rmu_sc0008941.1_g000005 Rmu_sc0008941.1_g000006 Rmu_sc0011173.1_g000001 Rmu_sc0011173.1_g000002 Rmu_sc0011173.1_g000008 Rmu_sc0011173.1_g000009 Rmu_sc0011173.1_g000013 Rmu_sc0011290.1_g000002 Rmu_sc0012908.1_g000002 Rmu_sc0013209.1_g000003 Rmu_sc0025395.1_g000001 Rmu_sc0025941.1_g000001 Rmu_sc0029089.1_g000001 Rmu_sc0036318.1_g000001 Rmu_sc0040213.1_g000001 Rmu_ssc0000092.1_g000038
rosa_roxburghii Rroxscaffold_3G00225010 Rroxscaffold_3G00225220 Rroxscaffold_3G00225300 Rroxscaffold_3G00225310 Rroxscaffold_3G00225390 Rroxscaffold_3G00225400 Rroxscaffold_3G00225470 Rroxscaffold_3G00225500 Rroxscaffold_3G00225570 Rroxscaffold_3G00225580 Rroxscaffold_3G00225600 Rroxscaffold_3G00225610 Rroxscaffold_3G00262350 Rroxscaffold_4G00313250
rosa_rugosa Rorug01G0145100.1 Rorug01G0145200.1 Rorug01G0145300.1 Rorug01G0145500.1 Rorug04G0161500 Rorug04G0161600 Rorug07G0007900 Rorug07G0008000 Rorug07G0008000 Rorug07G0273500 Rorug07G0273900 Rorug07G0274000 Rorug07G0274000 Rorug07G0274200.1 Rorug07G0274300 Rorug07G0274400 Rorug07G0293100 Rorug07G0293200 Rorug07G0293300 Rorug07G0293400 Rorug07G0293700 Rorug07G0293800 Rorug07G0294000 Rorug07G0294200 Rorug07G0294700 Rorug07G0294700 Rorug07G0295000 Rorug07G0295100 Rorug07G0295200 Rorug07G0295400 Rorug07G0295400 Rorug07G0296100 Rorug07G0297900 Rorug07G0297900 Rorug07G0298000 Rorug07G0298100
rosa_samantha Rh1DG165000 Rh1DG165100 Rh1DG165200 Rh1DG165600 Rh1DG165700 Rh1DG165800 Rh4DG221500 Rh7DG134800 Rh7DG134900 Rh7DG436900 Rh7DG437100 Rh7DG437300 Rh7DG437400 Rh7DG437500 Rh7DG437700 Rh7DG437800 Rh7DG438200 Rh7DG439200 Rh7DG439300 Rh7DG440300
rosa_wichuraiana Rw0G019880 Rw0G020650 Rw0G020670 Rw1G013620 Rw1G013630 Rw4G019180 Rw7G011360 Rw7G011370 Rw7G037220 Rw7G037240 Rw7G037260 Rw7G037270 Rw7G037280 Rw7G037300 Rw7G037380 Rw7G037390 Rw7G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
AasI GACNNNNNNGTC 2 cut(s) 245, 1607
Acc16I TGCGCA 2 cut(s) 666, 1852
Acc36I ACCTGC 1 cut(s) 893
AccB7I CCANNNNNTGG 2 cut(s) 256, 1358
AccI GTMKAC 2 cut(s) 384, 2001
AccII CGCG 1 cut(s) 1979
AccIII TCCGGA 1 cut(s) 678
AciI CCGC 2 cut(s) 5, 235
AclI AACGTT 1 cut(s) 582
AclWI GGATC 1 cut(s) 1532
AcsI RAATTY 1 cut(s) 2216
AcuI CTGAAG 2 cut(s) 1242, 1509
AdeI CACNNNGTG 1 cut(s) 256
AfaI GTAC 3 cut(s) 629, 2077, 2230
AfiI CCNNNNNNNGG 6 cut(s) 234, 256, 508, 1271, 1358, 1962
AflIII ACRYGT 1 cut(s) 2129
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 3 cut(s) 700, 885, 1237
AleI CACNNNNGTG 1 cut(s) 828
AloI GAACNNNNNNTCC 4 cut(s) 798, 830, 1281, 1313
AluBI AGCT 9 cut(s) 202, 880, 899, 1054, 1082, 1226, 1585, 1770, 2186
AluI AGCT 9 cut(s) 202, 880, 899, 1054, 1082, 1226, 1585, 1770, 2186
Alw21I GWGCWC 1 cut(s) 2188
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 1 cut(s) 1532
Ama87I CYCGRG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 678
AoxI GGCC 7 cut(s) 178, 209, 306, 519, 592, 703, 1019
ApeKI GCWGC 6 cut(s) 232, 498, 899, 953, 1028, 1079
ApoI RAATTY 1 cut(s) 2216
Asp700I GAANNNNTTC 1 cut(s) 2123
AspA2I CCTAGG 1 cut(s) 1804
AspLEI GCGC 3 cut(s) 667, 690, 1853
AspS9I GGNCC 4 cut(s) 178, 288, 1020, 1231
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 3 cut(s) 197, 754, 1253
AsuII TTCGAA 1 cut(s) 2049
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 2 cut(s) 288, 1231
AvrII CCTAGG 1 cut(s) 1804
BaeGI GKGCMC 1 cut(s) 1813
BanII GRGCYC 2 cut(s) 563, 2188
BauI CACGAG 1 cut(s) 2224
Bbv12I GWGCWC 1 cut(s) 2188
BbvCI CCTCAGC 1 cut(s) 1083
BbvI GCAGC 6 cut(s) 219, 485, 886, 940, 1015, 1091
BccI CCATC 5 cut(s) 743, 997, 1558, 1658, 1964
BceAI ACGGC 3 cut(s) 212, 1034, 1249
BciT130I CCWGG 3 cut(s) 702, 887, 1239
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 788
BfaI CTAG 8 cut(s) 12, 431, 758, 1556, 1586, 1673, 1805, 1883
BfmI CTRYAG 3 cut(s) 900, 1332, 1603
BfuAI ACCTGC 1 cut(s) 893
BglII AGATCT 1 cut(s) 1492
BisI GCNGC 6 cut(s) 233, 499, 900, 954, 1029, 1080
BlnI CCTAGG 1 cut(s) 1804
BlsI GCNGC 6 cut(s) 234, 500, 901, 955, 1030, 1081
Bme1390I CCNGG 4 cut(s) 503, 702, 887, 1239
Bme18I GGWCC 2 cut(s) 288, 1231
BmeT110I CYCGRG 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 178, 288, 1020, 1231
BmiI GGNNCC 1 cut(s) 1232
BmrFI CCNGG 4 cut(s) 503, 702, 887, 1239
BmrI ACTGGG 2 cut(s) 1853, 2167
BmsI GCATC 8 cut(s) 55, 652, 1331, 1389, 1459, 1838, 2150, 2152
BmuI ACTGGG 2 cut(s) 1853, 2167
Bpu10I CCTNAGC 1 cut(s) 1083
Bpu14I TTCGAA 1 cut(s) 2049
BpuEI CTTGAG 5 cut(s) 20, 287, 692, 833, 2077
BpuMI CCSGG 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 2232
BsaWI WCCGGW 2 cut(s) 678, 1529
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 1281, 1311
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 256, 508, 1271, 1358, 1962
Bse1I ACTGG 5 cut(s) 334, 1507, 1848, 2083, 2162
Bse3DI GCAATG 1 cut(s) 1408
BseAI TCCGGA 1 cut(s) 678
BseBI CCWGG 3 cut(s) 702, 887, 1239
BseLI CCNNNNNNNGG 6 cut(s) 234, 256, 508, 1271, 1358, 1962
BseMI GCAATG 1 cut(s) 1408
BseMII CTCAG 4 cut(s) 516, 1074, 1192, 2055
BseNI ACTGG 5 cut(s) 334, 1507, 1848, 2083, 2162
BseRI GAGGAG 2 cut(s) 1100, 1103
BseSI GKGCMC 1 cut(s) 1813
BseXI GCAGC 6 cut(s) 219, 485, 886, 940, 1015, 1091
BseYI CCCAGC 1 cut(s) 1226
Bsh1236I CGCG 1 cut(s) 1979
BshFI GGCC 7 cut(s) 180, 211, 308, 521, 594, 705, 1021
BsiHKAI GWGCWC 1 cut(s) 2188
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 502, 679, 1530
BslFI GGGAC 6 cut(s) 134, 251, 868, 960, 1244, 1979
BslI CCNNNNNNNGG 6 cut(s) 234, 256, 508, 1271, 1358, 1962
BsmAI GTCTC 1 cut(s) 788
BsmFI GGGAC 6 cut(s) 134, 251, 868, 960, 1244, 1979
BsmI GAATGC 1 cut(s) 1835
BsnI GGCC 7 cut(s) 180, 211, 308, 521, 594, 705, 1021
BsoBI CYCGRG 1 cut(s) 563
Bsp119I TTCGAA 1 cut(s) 2049
Bsp1286I GDGCHC 3 cut(s) 563, 1813, 2188
Bsp13I TCCGGA 1 cut(s) 678
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 4 cut(s) 729, 1492, 1537, 1579
Bsp19I CCATGG 2 cut(s) 768, 2114
BspACI CCGC 2 cut(s) 5, 235
BspANI GGCC 7 cut(s) 180, 211, 308, 521, 594, 705, 1021
BspCNI CTCAG 4 cut(s) 517, 1075, 1191, 2054
BspEI TCCGGA 1 cut(s) 678
BspFNI CGCG 1 cut(s) 1979
BspLI GGNNCC 1 cut(s) 1232
BspMAI CTGCAG 1 cut(s) 904
BspMI ACCTGC 1 cut(s) 893
BspPI GGATC 1 cut(s) 1532
BspT104I TTCGAA 1 cut(s) 2049
BsrDI GCAATG 1 cut(s) 1408
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 5 cut(s) 334, 1507, 1848, 2083, 2162
BssMI GATC 4 cut(s) 729, 1492, 1537, 1579
BssSI CACGAG 1 cut(s) 2224
BssT1I CCWWGG 7 cut(s) 291, 493, 549, 768, 1437, 1804, 2114
Bst2BI CACGAG 1 cut(s) 2224
Bst2UI CCWGG 3 cut(s) 702, 887, 1239
Bst4CI ACNGT 5 cut(s) 80, 256, 934, 1333, 1664
Bst6I CTCTTC 5 cut(s) 796, 1213, 1357, 1598, 1629
BstAPI GCANNNNNTGC 1 cut(s) 1456
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 2232
BstBI TTCGAA 1 cut(s) 2049
BstC8I GCNNGC 3 cut(s) 283, 992, 1461
BstDEI CTNAG 4 cut(s) 525, 1083, 1178, 2041
BstDSI CCRYGG 2 cut(s) 768, 2114
BstFNI CGCG 1 cut(s) 1979
BstHHI GCGC 3 cut(s) 667, 690, 1853
BstKTI GATC 4 cut(s) 732, 1495, 1540, 1582
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 4 cut(s) 729, 1492, 1537, 1579
BstMWI GCNNNNNNNGC 4 cut(s) 208, 614, 1136, 1456
BstNI CCWGG 3 cut(s) 702, 887, 1239
BstNSI RCATGY 1 cut(s) 2133
BstSCI CCNGG 4 cut(s) 501, 700, 885, 1237
BstSFI CTRYAG 3 cut(s) 900, 1332, 1603
BstSLI GKGCMC 1 cut(s) 1813
BstSNI TACGTA 1 cut(s) 2232
BstUI CGCG 1 cut(s) 1979
BstV1I GCAGC 6 cut(s) 219, 485, 886, 940, 1015, 1091
BstX2I RGATCY 1 cut(s) 1492
BstYI RGATCY 1 cut(s) 1492
BsuRI GGCC 7 cut(s) 180, 211, 308, 521, 594, 705, 1021
BtgI CCRYGG 2 cut(s) 768, 2114
BtgZI GCGATG 1 cut(s) 623
BtrI CACGTC 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 5 cut(s) 252, 327, 522, 1038, 1841
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 3 cut(s) 283, 992, 1461
CfoI GCGC 3 cut(s) 667, 690, 1853
Cfr13I GGNCC 4 cut(s) 178, 288, 1020, 1231
CseI GACGC 1 cut(s) 1985
CsiI ACCWGGT 1 cut(s) 1237
Csp6I GTAC 3 cut(s) 628, 2076, 2229
CviAII CATG 6 cut(s) 44, 94, 319, 769, 2115, 2130
CviQI GTAC 3 cut(s) 628, 2076, 2229
DdeI CTNAG 4 cut(s) 525, 1083, 1178, 2041
DpnI GATC 4 cut(s) 731, 1494, 1539, 1581
DpnII GATC 4 cut(s) 729, 1492, 1537, 1579
DraI TTTAAA 1 cut(s) 1788
DraIII CACNNNGTG 1 cut(s) 256
DrdI GACNNNNNNGTC 2 cut(s) 245, 1607
DseDI GACNNNNNNGTC 2 cut(s) 245, 1607
Eam1104I CTCTTC 5 cut(s) 796, 1213, 1357, 1598, 1629
EarI CTCTTC 5 cut(s) 796, 1213, 1357, 1598, 1629
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 2186
Eco105I TACGTA 1 cut(s) 2232
Eco130I CCWWGG 7 cut(s) 291, 493, 549, 768, 1437, 1804, 2114
Eco147I AGGCCT 2 cut(s) 211, 308
Eco24I GRGCYC 2 cut(s) 563, 2188
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 288, 1231
Eco53kI GAGCTC 1 cut(s) 2186
Eco57I CTGAAG 2 cut(s) 1242, 1509
Eco88I CYCGRG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 2186
EcoRII CCWGG 3 cut(s) 700, 885, 1237
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 7 cut(s) 291, 493, 549, 768, 1437, 1804, 2114
EcoT22I ATGCAT 1 cut(s) 2143
EcoT38I GRGCYC 2 cut(s) 563, 2188
ErhI CCWWGG 7 cut(s) 291, 493, 549, 768, 1437, 1804, 2114
FaeI CATG 6 cut(s) 47, 97, 322, 772, 2118, 2133
FalI AAGNNNNNCTT 6 cut(s) 793, 825, 974, 1006, 1439, 1471
FaqI GGGAC 6 cut(s) 134, 251, 868, 960, 1244, 1979
FatI CATG 6 cut(s) 43, 93, 318, 768, 2114, 2129
FauI CCCGC 1 cut(s) 228
FauNDI CATATG 1 cut(s) 1750
FbaI TGATCA 1 cut(s) 729
FblI GTMKAC 2 cut(s) 384, 2001
Fnu4HI GCNGC 6 cut(s) 233, 499, 900, 954, 1029, 1080
FriOI GRGCYC 2 cut(s) 563, 2188
Fsp4HI GCNGC 6 cut(s) 233, 499, 900, 954, 1029, 1080
FspAI RTGCGCAY 1 cut(s) 1852
FspBI CTAG 8 cut(s) 12, 431, 758, 1556, 1586, 1673, 1805, 1883
FspI TGCGCA 2 cut(s) 666, 1852
GlaI GCGC 3 cut(s) 666, 689, 1852
GluI GCNGC 6 cut(s) 233, 499, 900, 954, 1029, 1080
GsaI CCCAGC 1 cut(s) 1230
HaeIII GGCC 7 cut(s) 180, 211, 308, 521, 594, 705, 1021
HapII CCGG 3 cut(s) 502, 679, 1530
HgaI GACGC 1 cut(s) 1985
HhaI GCGC 3 cut(s) 667, 690, 1853
Hin1II CATG 6 cut(s) 47, 97, 322, 772, 2118, 2133
Hin6I GCGC 3 cut(s) 665, 688, 1851
HinP1I GCGC 3 cut(s) 665, 688, 1851
HpaII CCGG 3 cut(s) 502, 679, 1530
HphI GGTGA 3 cut(s) 197, 754, 1253
Hpy166II GTNNAC 6 cut(s) 250, 385, 628, 630, 1414, 2002
Hpy188I TCNGA 8 cut(s) 220, 394, 1047, 1420, 1489, 1579, 1945, 2175
Hpy8I GTNNAC 6 cut(s) 250, 385, 628, 630, 1414, 2002
Hpy99I CGWCG 3 cut(s) 1264, 1818, 2201
HpyAV CCTTC 7 cut(s) 46, 511, 584, 607, 739, 1633, 1868
HpyCH4III ACNGT 5 cut(s) 80, 256, 934, 1333, 1664
HpyCH4IV ACGT 3 cut(s) 240, 582, 2231
HpyCH4V TGCA 8 cut(s) 374, 902, 1061, 1344, 1835, 1903, 2141, 2165
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 614, 1136, 1456
HpyF3I CTNAG 4 cut(s) 525, 1083, 1178, 2041
HpySE526I ACGT 3 cut(s) 240, 582, 2231
Hsp92II CATG 6 cut(s) 47, 97, 322, 772, 2118, 2133
HspAI GCGC 3 cut(s) 665, 688, 1851
Kpn2I TCCGGA 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 4 cut(s) 729, 1492, 1537, 1579
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 6 cut(s) 219, 485, 886, 940, 1015, 1091
LweI GCATC 8 cut(s) 55, 652, 1331, 1389, 1459, 1838, 2150, 2152
MabI ACCWGGT 1 cut(s) 1237
MaeI CTAG 8 cut(s) 12, 431, 758, 1556, 1586, 1673, 1805, 1883
MaeII ACGT 3 cut(s) 240, 582, 2231
MaeIII GTNAC 3 cut(s) 185, 2092, 2108
MalI GATC 4 cut(s) 731, 1494, 1539, 1581
MboI GATC 4 cut(s) 729, 1492, 1537, 1579
MflI RGATCY 1 cut(s) 1492
MhlI GDGCHC 3 cut(s) 563, 1813, 2188
MlyI GAGTC 6 cut(s) 122, 498, 515, 575, 942, 1617
MmeI TCCRAC 4 cut(s) 420, 1443, 1609, 1747
Mph1103I ATGCAT 1 cut(s) 2143
MroI TCCGGA 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 2123
MseI TTAA 6 cut(s) 135, 579, 837, 938, 1787, 1985
MslI CAYNNNNRTG 6 cut(s) 773, 828, 1427, 1749, 1846, 2128
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 3 cut(s) 502, 679, 1530
MspR9I CCNGG 4 cut(s) 503, 702, 887, 1239
Mva1269I GAATGC 1 cut(s) 1835
MvaI CCWGG 3 cut(s) 702, 887, 1239
MvnI CGCG 1 cut(s) 1979
MwoI GCNNNNNNNGC 4 cut(s) 208, 614, 1136, 1456
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 2 cut(s) 768, 2114
NdeI CATATG 1 cut(s) 1750
NdeII GATC 4 cut(s) 729, 1492, 1537, 1579
NlaIII CATG 6 cut(s) 47, 97, 322, 772, 2118, 2133
NlaIV GGNNCC 1 cut(s) 1232
NmuCI GTSAC 2 cut(s) 185, 2092
NsbI TGCGCA 2 cut(s) 666, 1852
NsiI ATGCAT 1 cut(s) 2143
NspI RCATGY 1 cut(s) 2133
NspV TTCGAA 1 cut(s) 2049
OliI CACNNNNGTG 1 cut(s) 828
PaqCI CACCTGC 1 cut(s) 893
PasI CCCWGGG 1 cut(s) 886
PceI AGGCCT 2 cut(s) 211, 308
PciI ACATGT 1 cut(s) 2129
PctI GAATGC 1 cut(s) 1835
PdmI GAANNNNTTC 1 cut(s) 2123
PfeI GAWTC 6 cut(s) 110, 764, 1292, 2046, 2119, 2193
PflMI CCANNNNNTGG 2 cut(s) 256, 1358
PkrI GCNGC 6 cut(s) 234, 500, 901, 955, 1030, 1081
PleI GAGTC 6 cut(s) 122, 497, 514, 574, 942, 1616
PpsI GAGTC 6 cut(s) 122, 497, 514, 574, 942, 1616
Ppu21I YACGTR 1 cut(s) 2232
PscI ACATGT 1 cut(s) 2129
Psp124BI GAGCTC 1 cut(s) 2188
Psp1406I AACGTT 1 cut(s) 582
Psp6I CCWGG 3 cut(s) 700, 885, 1237
PspFI CCCAGC 1 cut(s) 1226
PspGI CCWGG 3 cut(s) 700, 885, 1237
PspN4I GGNNCC 1 cut(s) 1232
PspPI GGNCC 4 cut(s) 178, 288, 1020, 1231
PstI CTGCAG 1 cut(s) 904
PsuI RGATCY 1 cut(s) 1492
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 3 cut(s) 629, 2077, 2230
RsaNI GTAC 3 cut(s) 628, 2076, 2229
RseI CAYNNNNRTG 6 cut(s) 773, 828, 1427, 1749, 1846, 2128
SacI GAGCTC 1 cut(s) 2188
SaqAI TTAA 6 cut(s) 135, 579, 837, 938, 1787, 1985
SatI GCNGC 6 cut(s) 233, 499, 900, 954, 1029, 1080
Sau3AI GATC 4 cut(s) 729, 1492, 1537, 1579
Sau96I GGNCC 4 cut(s) 178, 288, 1020, 1231
SchI GAGTC 6 cut(s) 122, 498, 515, 575, 942, 1617
ScrFI CCNGG 4 cut(s) 503, 702, 887, 1239
SduI GDGCHC 3 cut(s) 563, 1813, 2188
SexAI ACCWGGT 1 cut(s) 1237
SfaNI GCATC 8 cut(s) 55, 652, 1331, 1389, 1459, 1838, 2150, 2152
SfcI CTRYAG 3 cut(s) 900, 1332, 1603
SfuI TTCGAA 1 cut(s) 2049
SinI GGWCC 2 cut(s) 288, 1231
SmiMI CAYNNNNRTG 6 cut(s) 773, 828, 1427, 1749, 1846, 2128
SmlI CTYRAG 5 cut(s) 35, 302, 707, 848, 2056
SmoI CTYRAG 5 cut(s) 35, 302, 707, 848, 2056
SnaBI TACGTA 1 cut(s) 2232
SseBI AGGCCT 2 cut(s) 211, 308
SsiI CCGC 2 cut(s) 5, 235
SspMI CTAG 8 cut(s) 12, 431, 758, 1556, 1586, 1673, 1805, 1883
SstI GAGCTC 1 cut(s) 2188
StuI AGGCCT 2 cut(s) 211, 308
StyD4I CCNGG 4 cut(s) 501, 700, 885, 1237
StyI CCWWGG 7 cut(s) 291, 493, 549, 768, 1437, 1804, 2114
TaaI ACNGT 5 cut(s) 80, 256, 934, 1333, 1664
TaiI ACGT 3 cut(s) 243, 585, 2234
TaqI TCGA 3 cut(s) 108, 113, 2049
TaqII GACCGA 2 cut(s) 237, 1248
TatI WGTACW 1 cut(s) 627
TfiI GAWTC 6 cut(s) 110, 764, 1292, 2046, 2119, 2193
Tru1I TTAA 6 cut(s) 135, 579, 837, 938, 1787, 1985
Tru9I TTAA 6 cut(s) 135, 579, 837, 938, 1787, 1985
TscAI CASTG 5 cut(s) 259, 334, 529, 1045, 1848
TseFI GTSAC 2 cut(s) 185, 2092
TseI GCWGC 6 cut(s) 232, 498, 899, 953, 1028, 1079
Tsp45I GTSAC 2 cut(s) 185, 2092
TspDTI ATGAA 7 cut(s) 65, 110, 467, 626, 777, 2159, 2209
TspRI CASTG 5 cut(s) 259, 334, 529, 1045, 1848
Van91I CCANNNNNTGG 2 cut(s) 256, 1358
VpaK11BI GGWCC 2 cut(s) 288, 1231
XapI RAATTY 1 cut(s) 2216
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 1 cut(s) 2133
XmaJI CCTAGG 1 cut(s) 1804
XmiI GTMKAC 2 cut(s) 384, 2001
XmnI GAANNNNTTC 1 cut(s) 2123
XspI CTAG 8 cut(s) 12, 431, 758, 1556, 1586, 1673, 1805, 1883
Zsp2I ATGCAT 1 cut(s) 2143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.