RchiOBHm_Chr7g0235941

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000101
Physical Location & Seq
Forward (+)
60832888 .. 60833496
609 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21144

Sequence Viewer

Length: 609 bp
ATGTTCAAAGCCGATGGCAATTATGACCAGGCCATGGAGCTCTATAGGCGCAGTTGGGAAACAAACACAATTCCAGCGGTCGTACTTCACACCGGTGTTATTGGAGACTGTCTCGAGGCTGGCAATACCGAGTGTGCTCTGAAGGCTTATCGGACCATGTTAGTTACTGGGGTTGCCCCAAACTCCTATACATACACTGTTTTAATTAAGGGACTCACTACTGACCCCAATTTCTGTGGGGATGCCAAGAGGTGCTTGCTTGAGATGATGGAGAGGGGGATGAGATCCAATGCTGCTACATACACTACGGTGATTGAGGGATTTGCGAAGCAGGAGGACAAGGTAGCTGAGGAGGAGGGCAAACAGTTGGTGGAGGTGATGATGGGCAACGGGTTTGTGCCAAATGCAAAGGCCATGATGGAGGTTTTGAAGGGTATTTGGCTCCAAAACCAGTCTGGCGTGCAAACAGTCTCTTTTGAGTGCGCGAGCGTGCCAGCGCCGTGCAGTGCAGTTGTCGGGGCATCCCTTGACTTGACTTCTGAATCAAAGGATTATACCGTCCGATCGACTGGAAATTTTGATATGATGACCGTAGGACTGTTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

202

Amino Acids

22.06

Weight (kDa)

4.9

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 26 - 72 7.2e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28420
fragaria_vesca FvH4_3g36751 FvH4_4g16590 FvH4_4g16590 FvH4_4g16590 FvH4_5g05670 FvH4_5g05700 FvH4_5g31002 FvH4_5g31070 FvH4_5g31080 FvH4_5g31080 FvH4_5g31080 FvH4_5g31150 FvH4_5g32070 FvH4_5g32080 FvH4_5g34900 FvH4_5g34930 FvH4_5g34930 FvH4_5g34950 FvH4_5g35000 FvH4_5g35000 FvH4_5g35000 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35050 FvH4_5g35060 FvH4_5g35070 FvH4_5g35071 FvH4_5g35080 FvH4_5g35253 FvH4_5g35254 FvH4_5g35300 FvH4_5g35300 FvH4_5g35320 FvH4_5g35320 FvH4_5g35320 FvH4_5g35322 FvH4_5g35330 FvH4_6g08812 FvH4_7g08990 FvH4_7g08991
rosa_chinensis RchiOBHm_Chr0c11g0499321 RchiOBHm_Chr0c11g0499331 RchiOBHm_Chr0c11g0499401 RchiOBHm_Chr0c11g0499411 RchiOBHm_Chr0c11g0499461 RchiOBHm_Chr0c11g0499471 RchiOBHm_Chr0c11g0499481 RchiOBHm_Chr0c11g0499501 RchiOBHm_Chr0c11g0499511 RchiOBHm_Chr0c11g0499551 RchiOBHm_Chr1g0342791 RchiOBHm_Chr1g0342991 RchiOBHm_Chr4g0419921 RchiOBHm_Chr4g0420021 RchiOBHm_Chr7g0193131 RchiOBHm_Chr7g0218371 RchiOBHm_Chr7g0218381 RchiOBHm_Chr7g0235811 RchiOBHm_Chr7g0235841 RchiOBHm_Chr7g0235851 RchiOBHm_Chr7g0235871 RchiOBHm_Chr7g0235881 RchiOBHm_Chr7g0235901 RchiOBHm_Chr7g0235921 RchiOBHm_Chr7g0235931 RchiOBHm_Chr7g0235941 RchiOBHm_Chr7g0235971 RchiOBHm_Chr7g0235981 RchiOBHm_Chr7g0235991 RchiOBHm_Chr7g0236021 RchiOBHm_Chr7g0236031 RchiOBHm_Chr7g0236041 RchiOBHm_Chr7g0236051 RchiOBHm_Chr7g0236071 RchiOBHm_Chr7g0236101 RchiOBHm_Chr7g0236111 RchiOBHm_Chr7g0236191 RchiOBHm_Chr7g0236201 RchiOBHm_Chr7g0236231 RchiOBHm_Chr7g0236241 RchiOBHm_Chr7g0236331 RchiOBHm_Chr7g0236591 RchiOBHm_Chr7g0236621 RchiOBHm_Chr7g0236631
rosa_laevigata RLG00000000990 RLG00000000992 RLG00000001071 RLG00000001073 RLG00000001074 RLG00000001075 RLG00000001083 RLG00000001096 RLG00000001097 RLG00000001099 RLG00000001106 RLG00000001108 RLG00000001110 RLG00000001112 RLG00000001119 RLG00000001120 RLG00000001123 RLG00000001128 RLG00000001129 RLG00000001130 RLG00000001131 RLG00000001132 RLG00000001133 RLG00000001136 RLG00000001255 RLG00000001929 RLG00000001930 RLG00000001932 RLG00000001934 RLG00000001935 RLG00000001940 RLG00000001942 RLG00000002624 RLG00000004293 RLG00000004294 RLG00000007792 RLG00000030009 RLG00000030011 RLG00000030012 RLG00000030014
rosa_multiflora Rmu_co8114088.1_g000001 Rmu_co8163812.1_g000001 Rmu_co8175782.1_g000001 Rmu_co8217500.1_g000001 Rmu_co8230787.1_g000001 Rmu_co8372547.1_g000001 Rmu_co8409897.1_g000001 Rmu_sc0000755.1_g000001 Rmu_sc0000755.1_g000003 Rmu_sc0002449.1_g000024 Rmu_sc0002536.1_g000007 Rmu_sc0002536.1_g000008 Rmu_sc0002536.1_g000013 Rmu_sc0002536.1_g000014 Rmu_sc0002921.1_g000004 Rmu_sc0002921.1_g000005 Rmu_sc0002921.1_g000007 Rmu_sc0003339.1_g000004 Rmu_sc0003461.1_g000006 Rmu_sc0005998.1_g000001 Rmu_sc0008183.1_g000003 Rmu_sc0008183.1_g000007 Rmu_sc0008183.1_g000009 Rmu_sc0008183.1_g000010 Rmu_sc0008183.1_g000011 Rmu_sc0008183.1_g000020 Rmu_sc0008183.1_g000022 Rmu_sc0008183.1_g000026 Rmu_sc0008940.1_g000007 Rmu_sc0008941.1_g000001 Rmu_sc0008941.1_g000002 Rmu_sc0008941.1_g000004 Rmu_sc0008941.1_g000005 Rmu_sc0008941.1_g000006 Rmu_sc0011173.1_g000001 Rmu_sc0011173.1_g000002 Rmu_sc0011173.1_g000008 Rmu_sc0011173.1_g000009 Rmu_sc0011173.1_g000013 Rmu_sc0011290.1_g000002 Rmu_sc0012908.1_g000002 Rmu_sc0013209.1_g000003 Rmu_sc0025395.1_g000001 Rmu_sc0025941.1_g000001 Rmu_sc0029089.1_g000001 Rmu_sc0036318.1_g000001 Rmu_sc0040213.1_g000001 Rmu_ssc0000092.1_g000038
rosa_roxburghii Rroxscaffold_3G00225010 Rroxscaffold_3G00225220 Rroxscaffold_3G00225300 Rroxscaffold_3G00225310 Rroxscaffold_3G00225390 Rroxscaffold_3G00225400 Rroxscaffold_3G00225470 Rroxscaffold_3G00225500 Rroxscaffold_3G00225570 Rroxscaffold_3G00225580 Rroxscaffold_3G00225600 Rroxscaffold_3G00225610 Rroxscaffold_3G00262350 Rroxscaffold_4G00313250
rosa_rugosa Rorug01G0145100.1 Rorug01G0145200.1 Rorug01G0145300.1 Rorug01G0145500.1 Rorug04G0161500 Rorug04G0161600 Rorug07G0007900 Rorug07G0008000 Rorug07G0008000 Rorug07G0273500 Rorug07G0273900 Rorug07G0274000 Rorug07G0274000 Rorug07G0274200.1 Rorug07G0274300 Rorug07G0274400 Rorug07G0293100 Rorug07G0293200 Rorug07G0293300 Rorug07G0293400 Rorug07G0293700 Rorug07G0293800 Rorug07G0294000 Rorug07G0294200 Rorug07G0294700 Rorug07G0294700 Rorug07G0295000 Rorug07G0295100 Rorug07G0295200 Rorug07G0295400 Rorug07G0295400 Rorug07G0296100 Rorug07G0297900 Rorug07G0297900 Rorug07G0298000 Rorug07G0298100
rosa_samantha Rh1DG165000 Rh1DG165100 Rh1DG165200 Rh1DG165600 Rh1DG165700 Rh1DG165800 Rh4DG221500 Rh7DG134800 Rh7DG134900 Rh7DG436900 Rh7DG437100 Rh7DG437300 Rh7DG437400 Rh7DG437500 Rh7DG437700 Rh7DG437800 Rh7DG438200 Rh7DG439200 Rh7DG439300 Rh7DG440300
rosa_wichuraiana Rw0G019880 Rw0G020650 Rw0G020670 Rw1G013620 Rw1G013630 Rw4G019180 Rw7G011360 Rw7G011370 Rw7G037220 Rw7G037240 Rw7G037260 Rw7G037270 Rw7G037280 Rw7G037300 Rw7G037380 Rw7G037390 Rw7G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 34
AccII CGCG 1 cut(s) 485
AciI CCGC 1 cut(s) 77
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 34
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 2 cut(s) 7, 430
AjnI CCWGG 1 cut(s) 27
AleI CACNNNNGTG 2 cut(s) 93, 308
AluBI AGCT 2 cut(s) 40, 347
AluI AGCT 2 cut(s) 40, 347
Alw21I GWGCWC 2 cut(s) 42, 139
Alw26I GTCTC 3 cut(s) 99, 116, 475
AlwI GGATC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 2 cut(s) 30, 411
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 574
AsiGI ACCGGT 1 cut(s) 92
AspLEI GCGC 3 cut(s) 51, 485, 499
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 322, 388
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 153
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 42, 139
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 280
BccI CCATC 4 cut(s) 8, 262, 376, 412
BceAI ACGGC 1 cut(s) 484
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 3 cut(s) 99, 116, 475
BfmI CTRYAG 1 cut(s) 43
BfoI RGCGCY 1 cut(s) 500
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 232, 530
BmuI ACTGGG 1 cut(s) 177
BplI GAGNNNNNCTC 2 cut(s) 96, 128
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 172, 451, 574
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 247, 285, 521
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 172, 451, 574
BseRI GAGGAG 2 cut(s) 365, 368
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 2 cut(s) 523, 528
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 2 cut(s) 81, 566
BshFI GGCC 2 cut(s) 32, 413
BshTI ACCGGT 1 cut(s) 92
BsiEI CGRYCG 2 cut(s) 81, 566
BsiHKAI GWGCWC 2 cut(s) 42, 139
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 3 cut(s) 99, 116, 475
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 2 cut(s) 32, 413
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 42, 139
Bsp143I GATC 2 cut(s) 284, 563
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 77
BspANI GGCC 2 cut(s) 32, 413
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 1 cut(s) 443
BspPI GGATC 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 172, 451, 574
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 2 cut(s) 284, 563
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 8 cut(s) 110, 199, 310, 366, 469, 559, 592, 600
BstC8I GCNNGC 6 cut(s) 121, 257, 461, 487, 491, 495
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 247, 285, 521
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 3 cut(s) 51, 485, 499
BstKTI GATC 2 cut(s) 287, 566
BstMAI GTCTC 3 cut(s) 99, 116, 475
BstMBI GATC 2 cut(s) 284, 563
BstMCI CGRYCG 2 cut(s) 81, 566
BstMWI GCNNNNNNNGC 2 cut(s) 46, 143
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 43
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuRI GGCC 2 cut(s) 32, 413
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 247, 285, 521
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 2 cut(s) 195, 511
Cac8I GCNNGC 6 cut(s) 121, 257, 461, 487, 491, 495
CfoI GCGC 3 cut(s) 51, 485, 499
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 83
CspAI ACCGGT 1 cut(s) 92
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviAII CATG 3 cut(s) 34, 157, 415
CviJI RGCY 8 cut(s) 11, 32, 40, 119, 146, 347, 413, 442
CviKI_1 RGCY 8 cut(s) 11, 32, 40, 119, 146, 347, 413, 442
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 286, 565
DpnII GATC 2 cut(s) 284, 563
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 37, 160, 418
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 33, 156, 414
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 3 cut(s) 254, 292, 508
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 294
GlaI GCGC 3 cut(s) 50, 484, 498
GluI GCNGC 1 cut(s) 294
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 2 cut(s) 32, 413
HapII CCGG 1 cut(s) 93
HhaI GCGC 3 cut(s) 51, 485, 499
Hin1II CATG 3 cut(s) 37, 160, 418
Hin6I GCGC 3 cut(s) 49, 483, 497
HinP1I GCGC 3 cut(s) 49, 483, 497
HinfI GANTC 2 cut(s) 213, 542
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 322, 388
Hpy188I TCNGA 4 cut(s) 141, 153, 541, 563
Hpy188III TCNNGA 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 136, 424
HpyCH4III ACNGT 8 cut(s) 110, 199, 310, 366, 469, 559, 592, 600
HpyCH4V TGCA 4 cut(s) 407, 463, 504, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 143
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 3 cut(s) 37, 160, 418
HspAI GCGC 3 cut(s) 49, 483, 497
Kzo9I GATC 2 cut(s) 284, 563
LmnI GCTCC 2 cut(s) 37, 447
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 2 cut(s) 232, 530
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 286, 565
MboI GATC 2 cut(s) 284, 563
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 42, 139
MluCI AATT 5 cut(s) 19, 69, 204, 229, 574
MlyI GAGTC 1 cut(s) 207
MseI TTAA 2 cut(s) 203, 207
MslI CAYNNNNRTG 2 cut(s) 93, 308
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 46, 143
NcoI CCATGG 1 cut(s) 33
NdeII GATC 2 cut(s) 284, 563
NlaIII CATG 3 cut(s) 37, 160, 418
NlaIV GGNNCC 1 cut(s) 443
OliI CACNNNNGTG 2 cut(s) 93, 308
PacI TTAATTAA 1 cut(s) 207
PaeR7I CTCGAG 1 cut(s) 113
PfeI GAWTC 1 cut(s) 542
PflMI CCANNNNNTGG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 1 cut(s) 295
Ple19I CGATCG 1 cut(s) 566
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 284
PvuI CGATCG 1 cut(s) 566
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 2 cut(s) 93, 308
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 2 cut(s) 203, 207
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 2 cut(s) 284, 563
Sau96I GGNCC 1 cut(s) 153
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 2 cut(s) 42, 139
SetI ASST 6 cut(s) 42, 254, 345, 349, 378, 426
SfaNI GCATC 2 cut(s) 232, 530
SfcI CTRYAG 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 113
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 93, 308
SmlI CTYRAG 2 cut(s) 113, 260
SmoI CTYRAG 2 cut(s) 113, 260
Sse9I AATT 5 cut(s) 19, 69, 204, 229, 574
SsiI CCGC 1 cut(s) 77
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 8 cut(s) 110, 199, 310, 366, 469, 559, 592, 600
TaqI TCGA 3 cut(s) 114, 566, 604
TasI AATT 5 cut(s) 19, 69, 204, 229, 574
TfiI GAWTC 1 cut(s) 542
Tru1I TTAA 2 cut(s) 203, 207
Tru9I TTAA 2 cut(s) 203, 207
TscAI CASTG 2 cut(s) 202, 511
TseI GCWGC 1 cut(s) 293
TspRI CASTG 2 cut(s) 202, 511
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.