AT1G32140

ABC transporter E family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000186
Physical Location & Seq
Reverse (-)
11562742 .. 11564833
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32140.1

Sequence Viewer

Length: 1776 bp
ATGACGATGATGTCCGACCTTTCACTTGATTTAGTCGAAGAGATATTGTGTAGGGTTCCGATAACTTCTCTTAAAGCAGTGAGATCTAGTTGCAAACTATGGAACGTTCTTTCCAAGAACCGGATTTTATGTAAAACAGAAGCTAGAAATCAGTTTTTAGGGTTCACGATAATGAATCATAGGCTTTATTCCATGAGATTCAATCTCCATGGAATCGGCCTCAATGAAAACAGTGAAGAGTTCATTGATCCATCTATAAAGCCAATAGGTAATTTACTTAATCAAGTCGAGATATCTAAAGTGTTTTATTGCGAAGGTTTATTGTTATGCGTCACAAGGAACCACTCAAGCAAGCTCGTAGTTTGGAACCCGTATTTGGGAGAAATTCGTTGGATCAAAACTAGGAATGATTACCACATAGGCGTTACATATGCTCTCGGGTACGACAACAACAAGAACCACATGATCTTGAGGTTTTTTTCTGAACAAGGCTACTACGAGATTTACGACATGAACTCTTCTGACTCATGGGATTGTTTTTATGGCATTCCCAACAAGGGGTTAAAATGTTATCAGCCCGGCGCGTCGTTAAATGGAAATGCTTATTTTTTGACTGAGGGAAGAGAAGTAATGGAAGGGTATGATTGCTTACTCGGTTTTGATTTTACAACAAAGAAATTTGGACCACTTCTTTCTTTGTCGTTTTCGCATGATTTTATAGAGACTGGGAGACTATCTTGTGTTAAAGGAGAGAAACTTGCGGTCTTATATCAGCGCTGCTATACCTATGAGATGGAGGTATGGGTGACAACTAAGATAGAGCCGAATGCGGTGTCATGGAGCAAATTCTTAGCAGTTGAAATGGAACCACTCACTAGTCTAAAGTTTAACGATGATTCTGGCAGTTTCTTCATTGACGAAGAGAAGAAAATCGTCGTGGTTTTTGATATAGACGAATCTGAACGCAACAATACGGCTTACATCATTGGAGATTATGGATGCTTGAAAGAAGTTGATCTTGATGAAGTTGTGAACCCACAAGAATCTGTGGAGGTCGGAGACCGCATTTATTCTTTTTCACCATTTGTGTGCTCTTGCTCTTATGTTCCAAGTTTAGTGAAATTTAAAGAAGATGCAGAACATGAAAGGAAAGATAAGAAGAGGAAGAGTAAGAGGAAGCGAACCAACAAGGATGGATATGATTTTATACTGTGTTTTGATTTTACAACCGAGAGATTTGGACAGATTCTTCCTCTGCCGTTTAAACATTCTTTTAGGGATACTTGGACTCTATCTTCTGTTAAAGAAGAGAAACTTGCAGTCGCAGTGTTATACTGGAAAAATACATGTGTGATGATAGAGATATGGATGACAATTAAGATTGATCCTAATGTCGAGTCGTGGAGCAAATTCTTAAGAGTTGATAGGAAACCATGCATTGATCTTCGCTTTGATGATCGTAATGACAGTTTTTTCATTGACGAAGAGAAGAAAGTTGTCGTGTTTTTTAGTTCAGACAAAGTTAAAACCTCTACGGCTTACGTCATTGGAGATAATAGATATTTGAGAACAGTGGATCTTGAAAAAGCTGCAAACTCCCAAGAATCTGTGGAGGTCGGAGAACGCGTGTATTGTTTTTCGCCGCTTGTGTGCTCTTGCTCTTATTATGTTCCAAGTTTGGTGAAAATCAACCACAATGCAGGACGCAAAAGGAAAGAGAAGAAGACGAAGCGCAAAAGTAAAGACAAGCAGATGAAACTAAGCAACAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_tran F-box FBA_1 Fer4 RLI

Protein Analysis

591

Amino Acids

68.65

Weight (kDa)

7.94

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 55 - 374 9.9e-99 F-box associated beta propeller domain
FBA_3 PF08268 104 - 295 5.3e-09 F-box associated beta propeller domain
FBA_1 PF07734 395 - 563 1.7e-41 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccII CGCG 2 cut(s) 584, 1626
AciI CCGC 4 cut(s) 761, 830, 1063, 1643
AclI AACGTT 1 cut(s) 105
AclWI GGATC 4 cut(s) 242, 401, 1379, 1584
AcsI RAATTY 5 cut(s) 384, 677, 845, 1121, 1409
AfaI GTAC 1 cut(s) 443
AfeI AGCGCT 1 cut(s) 776
AfiI CCNNNNNNNGG 5 cut(s) 120, 376, 377, 557, 558
AflII CTTAAG 1 cut(s) 1414
AflIII ACRYGT 2 cut(s) 1346, 1624
AgsI TTSAA 4 cut(s) 202, 860, 1006, 1583
AhlI ACTAGT 1 cut(s) 875
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 3 cut(s) 143, 355, 1589
AluI AGCT 3 cut(s) 143, 355, 1589
Alw21I GWGCWC 2 cut(s) 1094, 1655
Alw26I GTCTC 3 cut(s) 716, 724, 1053
AlwI GGATC 4 cut(s) 242, 401, 1379, 1584
Ama87I CYCGRG 1 cut(s) 437
Aor51HI AGCGCT 1 cut(s) 776
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 777, 1589
ApoI RAATTY 5 cut(s) 384, 677, 845, 1121, 1409
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AspLEI GCGC 3 cut(s) 584, 777, 1734
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 3 cut(s) 817, 1071, 1693
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 1730
Bbv12I GWGCWC 2 cut(s) 1094, 1655
BbvI GCAGC 2 cut(s) 764, 1576
BccI CCATC 3 cut(s) 259, 787, 1187
BceAI ACGGC 3 cut(s) 990, 1243, 1551
BciVI GTATCC 1 cut(s) 1273
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 3 cut(s) 716, 724, 1053
BcuI ACTAGT 1 cut(s) 875
BfaI CTAG 4 cut(s) 87, 144, 402, 876
BfoI RGCGCY 1 cut(s) 778
BfrI CTTAAG 1 cut(s) 1414
BfuI GTATCC 1 cut(s) 1273
BglII AGATCT 1 cut(s) 83
BisI GCNGC 3 cut(s) 778, 1590, 1643
BlsI GCNGC 3 cut(s) 779, 1591, 1644
Bme1390I CCNGG 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 683
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 4 cut(s) 57, 341, 368, 867
BmrFI CCNGG 1 cut(s) 579
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 2 cut(s) 989, 1123
BmuI ACTGGG 1 cut(s) 735
BpiI GAAGAC 1 cut(s) 1730
BpuEI CTTGAG 2 cut(s) 331, 490
BpuMI CCSGG 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 1053
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 1611, 1641
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 376, 377, 557, 558
Bse1I ACTGG 2 cut(s) 730, 1340
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 3 cut(s) 1004, 1198, 1374
BseLI CCNNNNNNNGG 5 cut(s) 120, 376, 377, 557, 558
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 730, 1340
BseXI GCAGC 2 cut(s) 764, 1576
Bsh1236I CGCG 2 cut(s) 584, 1626
BshFI GGCC 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 1094, 1655
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 2 cut(s) 121, 579
BslI CCNNNNNNNGG 5 cut(s) 120, 376, 377, 557, 558
BsmAI GTCTC 3 cut(s) 716, 724, 1053
BsmI GAATGC 2 cut(s) 546, 832
BsnI GGCC 1 cut(s) 219
Bso31I GGTCTC 1 cut(s) 1053
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 1094, 1655
Bsp143I GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 4 cut(s) 761, 830, 1063, 1643
BspANI GGCC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 2 cut(s) 584, 1626
BspLI GGNNCC 4 cut(s) 57, 341, 368, 867
BspPI GGATC 4 cut(s) 242, 401, 1379, 1584
BspTI CTTAAG 1 cut(s) 1414
BspTNI GGTCTC 1 cut(s) 1053
BsrI ACTGG 2 cut(s) 730, 1340
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 4 cut(s) 233, 1212, 1469, 1573
Bst6I CTCTTC 9 cut(s) 33, 231, 523, 616, 915, 1154, 1160, 1302, 1479
BstAFI CTTAAG 1 cut(s) 1414
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 4 cut(s) 615, 813, 850, 1760
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 3 cut(s) 1004, 1198, 1374
BstFNI CGCG 2 cut(s) 584, 1626
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 3 cut(s) 584, 777, 1734
BstKTI GATC 9 cut(s) 86, 250, 396, 468, 1018, 1387, 1444, 1459, 1579
BstMAI GTCTC 3 cut(s) 716, 724, 1053
BstMBI GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
BstNSI RCATGY 1 cut(s) 1350
BstSCI CCNGG 1 cut(s) 577
BstUI CGCG 2 cut(s) 584, 1626
BstV1I GCAGC 2 cut(s) 764, 1576
BstV2I GAAGAC 1 cut(s) 1730
BstX2I RGATCY 2 cut(s) 83, 1576
BstYI RGATCY 2 cut(s) 83, 1576
BsuI GTATCC 1 cut(s) 1273
BsuRI GGCC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 3 cut(s) 1004, 1198, 1374
BtsI GCAGTG 2 cut(s) 84, 1332
BtsIMutI CAGTG 4 cut(s) 84, 238, 1332, 1578
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 3 cut(s) 584, 777, 1734
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 3 cut(s) 319, 573, 1713
Csp6I GTAC 1 cut(s) 442
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 4 cut(s) 615, 813, 850, 1760
DpnI GATC 9 cut(s) 85, 249, 395, 467, 1017, 1386, 1443, 1458, 1578
DpnII GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
DraI TTTAAA 2 cut(s) 1126, 1264
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 9 cut(s) 33, 231, 523, 616, 915, 1154, 1160, 1302, 1479
EarI CTCTTC 9 cut(s) 33, 231, 523, 616, 915, 1154, 1160, 1302, 1479
Eco130I CCWWGG 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 1053
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 683
Eco47III AGCGCT 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 437
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 1439
ErhI CCWWGG 1 cut(s) 208
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 1299, 1331
FauNDI CATATG 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 778, 1590, 1643
FokI GGATG 3 cut(s) 1011, 1205, 1381
Fsp4HI GCNGC 3 cut(s) 778, 1590, 1643
FspBI CTAG 4 cut(s) 87, 144, 402, 876
GlaI GCGC 3 cut(s) 583, 776, 1733
GluI GCNGC 3 cut(s) 778, 1590, 1643
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 1 cut(s) 219
HapII CCGG 2 cut(s) 121, 579
HgaI GACGC 3 cut(s) 319, 573, 1713
HhaI GCGC 3 cut(s) 584, 777, 1734
Hin6I GCGC 3 cut(s) 582, 775, 1732
HinP1I GCGC 3 cut(s) 582, 775, 1732
HpaII CCGG 2 cut(s) 121, 579
HphI GGTGA 3 cut(s) 817, 1071, 1693
Hpy166II GTNNAC 2 cut(s) 165, 1033
Hpy188I TCNGA 8 cut(s) 16, 60, 484, 523, 961, 1058, 1516, 1619
Hpy188III TCNNGA 5 cut(s) 166, 289, 469, 1019, 1580
Hpy8I GTNNAC 2 cut(s) 165, 1033
Hpy99I CGWCG 2 cut(s) 589, 938
HpyAV CCTTC 2 cut(s) 308, 629
HpyCH4III ACNGT 4 cut(s) 233, 1212, 1469, 1573
HpyCH4IV ACGT 2 cut(s) 105, 1542
HpyCH4V TGCA 6 cut(s) 93, 1136, 1319, 1437, 1592, 1700
HpyF3I CTNAG 4 cut(s) 615, 813, 850, 1760
HpySE526I ACGT 2 cut(s) 105, 1542
HspAI GCGC 3 cut(s) 582, 775, 1732
Kzo9I GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
LmnI GCTCC 2 cut(s) 840, 1404
LpnPI CCDG 6 cut(s) 134, 592, 711, 885, 1321, 1686
Lsp1109I GCAGC 2 cut(s) 764, 1576
LweI GCATC 2 cut(s) 989, 1123
MaeI CTAG 4 cut(s) 87, 144, 402, 876
MaeII ACGT 2 cut(s) 105, 1542
MaeIII GTNAC 3 cut(s) 331, 424, 805
MalI GATC 9 cut(s) 85, 249, 395, 467, 1017, 1386, 1443, 1458, 1578
MboI GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
MflI RGATCY 2 cut(s) 83, 1576
MhlI GDGCHC 2 cut(s) 1094, 1655
MluCI AATT 7 cut(s) 271, 384, 677, 845, 1121, 1374, 1409
MluI ACGCGT 1 cut(s) 1624
MlyI GAGTC 3 cut(s) 518, 1282, 1406
MmeI TCCRAC 4 cut(s) 39, 371, 1036, 1597
Mph1103I ATGCAT 1 cut(s) 1439
MslI CAYNNNNRTG 2 cut(s) 170, 1087
MspCI CTTAAG 1 cut(s) 1414
MspI CCGG 2 cut(s) 121, 579
MspR9I CCNGG 1 cut(s) 579
MssI GTTTAAAC 1 cut(s) 1264
Mva1269I GAATGC 2 cut(s) 546, 832
MvnI CGCG 2 cut(s) 584, 1626
NciI CCSGG 1 cut(s) 579
NcoI CCATGG 1 cut(s) 208
NdeI CATATG 1 cut(s) 430
NdeII GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
NlaIV GGNNCC 4 cut(s) 57, 341, 368, 867
NmuCI GTSAC 2 cut(s) 331, 805
NsiI ATGCAT 1 cut(s) 1439
NspI RCATGY 1 cut(s) 1350
PciI ACATGT 1 cut(s) 1346
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 1623
PctI GAATGC 2 cut(s) 546, 832
PfeI GAWTC 8 cut(s) 175, 198, 213, 896, 956, 1043, 1246, 1604
PkrI GCNGC 3 cut(s) 779, 1591, 1644
PleI GAGTC 3 cut(s) 518, 1282, 1405
PmeI GTTTAAAC 1 cut(s) 1264
PpsI GAGTC 3 cut(s) 518, 1282, 1405
PscI ACATGT 1 cut(s) 1346
Psp1406I AACGTT 1 cut(s) 105
PspN4I GGNNCC 4 cut(s) 57, 341, 368, 867
PspPI GGNCC 1 cut(s) 683
PsuI RGATCY 2 cut(s) 83, 1576
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 2 cut(s) 170, 1087
SatI GCNGC 3 cut(s) 778, 1590, 1643
Sau3AI GATC 9 cut(s) 83, 247, 393, 465, 1015, 1384, 1441, 1456, 1576
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 3 cut(s) 518, 1282, 1406
ScrFI CCNGG 1 cut(s) 579
SduI GDGCHC 2 cut(s) 1094, 1655
SfaNI GCATC 2 cut(s) 989, 1123
SinI GGWCC 1 cut(s) 683
SmiMI CAYNNNNRTG 2 cut(s) 170, 1087
SmlI CTYRAG 3 cut(s) 346, 469, 1414
SmoI CTYRAG 3 cut(s) 346, 469, 1414
SpeI ACTAGT 1 cut(s) 875
Sse9I AATT 7 cut(s) 271, 384, 677, 845, 1121, 1374, 1409
SsiI CCGC 4 cut(s) 761, 830, 1063, 1643
SspMI CTAG 4 cut(s) 87, 144, 402, 876
StyD4I CCNGG 1 cut(s) 577
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 4 cut(s) 233, 1212, 1469, 1573
TaiI ACGT 2 cut(s) 108, 1545
TaqI TCGA 3 cut(s) 36, 288, 1395
TasI AATT 7 cut(s) 271, 384, 677, 845, 1121, 1374, 1409
TauI GCSGC 1 cut(s) 1645
TfiI GAWTC 8 cut(s) 175, 198, 213, 896, 956, 1043, 1246, 1604
TscAI CASTG 4 cut(s) 84, 238, 1332, 1578
TseFI GTSAC 2 cut(s) 331, 805
TseI GCWGC 2 cut(s) 777, 1589
Tsp45I GTSAC 2 cut(s) 331, 805
TspDTI ATGAA 9 cut(s) 188, 232, 240, 527, 901, 1038, 1158, 1465, 1769
TspRI CASTG 4 cut(s) 84, 238, 1332, 1578
Vha464I CTTAAG 1 cut(s) 1414
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 5 cut(s) 384, 677, 845, 1121, 1409
XceI RCATGY 1 cut(s) 1350
XspI CTAG 4 cut(s) 87, 144, 402, 876
Zsp2I ATGCAT 1 cut(s) 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.