AT3G20030

ABC transporter E family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
6990254 .. 6991462
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20030.1

Sequence Viewer

Length: 1209 bp
ATGACGATGATGAGTGATCTTTCGCAGGATTTGTTAGAGGAGATATTGTCTAGGGTTCCGAGAACTTCTCTAGGAGCAGTACGATCCACTTGCAAACGGTGGAACACTTTATTCAAAGATCGAATTCTATGTAAAGCAGAAGAAACAAGGGATCAGTTTCGGTTCATAATGAAGAAGTATAAGCTTTGTTCAATGAGGTTTGATCTCAACGGAACCTTAAACGAAGATGGAGGTACCGAATTCGTGGATCCATCTATAAAGGAGTTAGGTCATTTTTTTAATCAAGTCAAGATATCTAAAGTTTTTCAATGCGACGGGTTATTGTTGTGCGTGACCAAAGAGGACAACATTAGGCTCGTGGTATGGAATCCTTATTTGGGTCAAATCAGGTGGATCGAATCCGGTAACACTAATTACCGCCTATTCGACAGGTATGCTATCGGATACGACAATAACCGTAAACACAAAATCTTGAGGTTTCTGGAATATTACAACTTTAACATGAAGCACAGATTTCTTGAGTACGAAATCTACGATTTTAGCTCTAATTCATGGAGGGTTCTTGATATCGCTCCCCGTTGGGAAATAGAGTCTTATCAACGTGGAGCGTCTTTGAAAGGAAACACTTACTTTATTGCCAAGGAAAAAATAGAATACGAAGAGGATGGAGAGTTTCCAGAGCCGGCAGATAATTTACTCTGTTTTGATTTTACGACGGAGAGCTTTGGACAGTTTCTTCCTCTGCCGTTTCAGCACTATCTTTACGATGTTGGAGCTCTCTCTTCTCTTGGAGATGAGAAACTTGCGGCGTTATTTCAGTGCGGGGATACCGATTTATCAGAGGTGGAGATTTGGGTTACAACTTTGACTGAGACCAATACAGTGTCATGGAATCCTTTCTTAAAAGTTGATATGGAACCACACTACGGTCGTAGTTTTATGTTTGACTATTACGGTGGCAGTTTCTTCATTGACGAGGAGAAGAAACTCGCTGTTGTTTTTCAGTTTGACGAATCTGGAATGACCCGCTACGAGGACGCAACTTACATCATTGGAGAGAATGGCTACGTTAAAAAAGTGCGTCTTGGAGAAGCTCCTGCGAATCAAGGAGGATATTGTTTCCCATCTGTGTGTTTTAGCTCTTATGTTCCAAGTTTAGTACAGATCAACCAAATTGTAGGATTCAAAAAAGAAAGAGAAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_tran F-box FBA_1 Fer4 RLI

Protein Analysis

402

Amino Acids

47.02

Weight (kDa)

5.02

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 43 4e-09 F-box domain
FBA_1 PF07734 55 - 389 5.3e-108 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 4 cut(s) 418, 806, 822, 1027
AclWI GGATC 5 cut(s) 78, 159, 242, 255, 401
AcsI RAATTY 2 cut(s) 123, 239
AfaI GTAC 4 cut(s) 81, 235, 524, 1161
AfiI CCNNNNNNNGG 3 cut(s) 377, 926, 1033
AgsI TTSAA 5 cut(s) 115, 192, 308, 616, 1186
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 6 cut(s) 184, 543, 723, 776, 1094, 1140
AluI AGCT 6 cut(s) 184, 543, 723, 776, 1094, 1140
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 866
AlwI GGATC 5 cut(s) 78, 159, 242, 255, 401
ApoI RAATTY 2 cut(s) 123, 239
Asp700I GAANNNNTTC 1 cut(s) 896
Asp718I GGTACC 1 cut(s) 233
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 778
BauI CACGAG 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 778
BccI CCATC 4 cut(s) 221, 259, 659, 1132
BceAI ACGGC 1 cut(s) 730
BcgI CGANNNNNNTGC 4 cut(s) 72, 106, 416, 450
BciVI GTATCC 2 cut(s) 437, 820
BcoDI GTCTC 1 cut(s) 866
BfaI CTAG 2 cut(s) 51, 71
BfuI GTATCC 2 cut(s) 437, 820
BisI GCNGC 1 cut(s) 807
BlsI GCNGC 1 cut(s) 808
BmiI GGNNCC 5 cut(s) 57, 214, 235, 249, 918
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpuEI CTTGAG 2 cut(s) 493, 539
BsaI GGTCTC 1 cut(s) 866
BsaJI CCNNGG 1 cut(s) 639
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 926, 1033
Bse118I RCCGGY 1 cut(s) 682
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 3 cut(s) 377, 926, 1033
BseMII CTCAG 1 cut(s) 861
BseRI GAGGAG 2 cut(s) 53, 992
Bsh1285I CGRYCG 1 cut(s) 931
BshNI GGYRCC 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 931
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 2 cut(s) 402, 683
BslI CCNNNNNNNGG 3 cut(s) 377, 926, 1033
BsmAI GTCTC 1 cut(s) 866
Bso31I GGTCTC 1 cut(s) 866
Bsp1286I GDGCHC 1 cut(s) 778
Bsp143I GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
BspACI CCGC 4 cut(s) 418, 806, 822, 1027
BspCNI CTCAG 1 cut(s) 862
BspLI GGNNCC 5 cut(s) 57, 214, 235, 249, 918
BspPI GGATC 5 cut(s) 78, 159, 242, 255, 401
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 866
BsrFI RCCGGY 1 cut(s) 682
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 356
Bst4CI ACNGT 6 cut(s) 99, 458, 732, 883, 929, 956
Bst6I CTCTTC 3 cut(s) 654, 787, 1197
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 1 cut(s) 870
BstF5I GGATG 1 cut(s) 670
BstKTI GATC 8 cut(s) 19, 86, 121, 154, 205, 250, 396, 1167
BstMAI GTCTC 1 cut(s) 866
BstMBI GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
BstMCI CGRYCG 1 cut(s) 931
BstMWI GCNNNNNNNGC 1 cut(s) 751
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuI GTATCC 2 cut(s) 437, 820
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 2 cut(s) 824, 888
Cac8I GCNNGC 1 cut(s) 684
Cfr10I RCCGGY 1 cut(s) 682
CseI GACGC 3 cut(s) 597, 1046, 1070
Csp6I GTAC 4 cut(s) 80, 234, 523, 1160
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 3 cut(s) 502, 552, 888
CviJI RGCY 9 cut(s) 184, 355, 543, 682, 723, 776, 1065, 1094, 1140
CviKI_1 RGCY 9 cut(s) 184, 355, 543, 682, 723, 776, 1065, 1094, 1140
CviQI GTAC 4 cut(s) 80, 234, 523, 1160
DdeI CTNAG 1 cut(s) 870
DpnI GATC 8 cut(s) 18, 85, 120, 153, 204, 249, 395, 1166
DpnII GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
Eam1104I CTCTTC 3 cut(s) 654, 787, 1197
EarI CTCTTC 3 cut(s) 654, 787, 1197
Ecl136II GAGCTC 1 cut(s) 776
Eco130I CCWWGG 1 cut(s) 639
Eco24I GRGCYC 1 cut(s) 778
Eco31I GGTCTC 1 cut(s) 866
Eco32I GATATC 2 cut(s) 294, 568
Eco53kI GAGCTC 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 776
EcoRI GAATTC 2 cut(s) 123, 239
EcoRV GATATC 2 cut(s) 294, 568
EcoT14I CCWWGG 1 cut(s) 639
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 3 cut(s) 505, 555, 891
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FatI CATG 3 cut(s) 501, 551, 887
FauI CCCGC 2 cut(s) 815, 1034
Fnu4HI GCNGC 1 cut(s) 807
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 1 cut(s) 778
Fsp4HI GCNGC 1 cut(s) 807
FspBI CTAG 2 cut(s) 51, 71
GluI GCNGC 1 cut(s) 807
HapII CCGG 2 cut(s) 402, 683
HgaI GACGC 3 cut(s) 597, 1046, 1070
Hin1II CATG 3 cut(s) 505, 555, 891
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 7 cut(s) 367, 398, 590, 892, 1013, 1102, 1182
HpaII CCGG 2 cut(s) 402, 683
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 3 cut(s) 60, 443, 841
Hpy188III TCNNGA 7 cut(s) 289, 472, 482, 518, 563, 677, 1017
Hpy8I GTNNAC 1 cut(s) 461
Hpy99I CGWCG 2 cut(s) 317, 718
HpyCH4III ACNGT 6 cut(s) 99, 458, 732, 883, 929, 956
HpyCH4IV ACGT 2 cut(s) 601, 1068
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 1 cut(s) 751
HpyF3I CTNAG 1 cut(s) 870
HpySE526I ACGT 2 cut(s) 601, 1068
Hsp92II CATG 3 cut(s) 505, 555, 891
KpnI GGTACC 1 cut(s) 237
KroI GCCGGC 1 cut(s) 682
KroNI GCCGGC 1 cut(s) 684
Kzo9I GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
LmnI GCTCC 5 cut(s) 74, 577, 605, 773, 1099
LpnPI CCDG 9 cut(s) 11, 373, 415, 415, 467, 690, 696, 1002, 1110
MaeI CTAG 2 cut(s) 51, 71
MaeII ACGT 2 cut(s) 601, 1068
MaeIII GTNAC 3 cut(s) 331, 404, 856
MalI GATC 8 cut(s) 18, 85, 120, 153, 204, 249, 395, 1166
MboI GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
MboII GAAGA 8 cut(s) 152, 184, 236, 671, 728, 774, 958, 994
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 1 cut(s) 778
MluCI AATT 6 cut(s) 123, 239, 412, 547, 691, 1173
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 1 cut(s) 751
MroNI GCCGGC 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 5 cut(s) 218, 279, 498, 902, 1071
MslI CAYNNNNRTG 1 cut(s) 1129
MspI CCGG 2 cut(s) 402, 683
MwoI GCNNNNNNNGC 1 cut(s) 751
NaeI GCCGGC 1 cut(s) 684
NdeII GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
NgoMIV GCCGGC 1 cut(s) 682
NlaIII CATG 3 cut(s) 505, 555, 891
NlaIV GGNNCC 5 cut(s) 57, 214, 235, 249, 918
NmuCI GTSAC 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PdiI GCCGGC 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 6 cut(s) 367, 398, 892, 1013, 1102, 1182
PkrI GCNGC 1 cut(s) 808
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Psp124BI GAGCTC 1 cut(s) 778
PspN4I GGNNCC 5 cut(s) 57, 214, 235, 249, 918
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 4 cut(s) 81, 235, 524, 1161
RsaNI GTAC 4 cut(s) 80, 234, 523, 1160
RseI CAYNNNNRTG 1 cut(s) 1129
SacI GAGCTC 1 cut(s) 778
SaqAI TTAA 5 cut(s) 218, 279, 498, 902, 1071
SatI GCNGC 1 cut(s) 807
Sau3AI GATC 8 cut(s) 16, 83, 118, 151, 202, 247, 393, 1164
SchI GAGTC 1 cut(s) 599
SduI GDGCHC 1 cut(s) 778
SmiMI CAYNNNNRTG 1 cut(s) 1129
SmlI CTYRAG 2 cut(s) 472, 518
SmoI CTYRAG 2 cut(s) 472, 518
Sse9I AATT 6 cut(s) 123, 239, 412, 547, 691, 1173
SsiI CCGC 4 cut(s) 418, 806, 822, 1027
SspI AATATT 1 cut(s) 488
SspMI CTAG 2 cut(s) 51, 71
SstI GAGCTC 1 cut(s) 778
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 6 cut(s) 99, 458, 732, 883, 929, 956
TaiI ACGT 2 cut(s) 604, 1071
TaqI TCGA 3 cut(s) 121, 396, 426
TasI AATT 6 cut(s) 123, 239, 412, 547, 691, 1173
TatI WGTACW 1 cut(s) 1159
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 6 cut(s) 367, 398, 892, 1013, 1102, 1182
Tru1I TTAA 5 cut(s) 218, 279, 498, 902, 1071
Tru9I TTAA 5 cut(s) 218, 279, 498, 902, 1071
TscAI CASTG 2 cut(s) 824, 888
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 5 cut(s) 154, 185, 518, 540, 958
TspGWI ACGGA 2 cut(s) 225, 731
TspRI CASTG 2 cut(s) 824, 888
XapI RAATTY 2 cut(s) 123, 239
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 2 cut(s) 51, 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.