AT3G22650

F-box LRR-repeat kelch-repeat protein At1g11620

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
8014809 .. 8015960
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22650.2

Sequence Viewer

Length: 1119 bp
ATGGCTTCATGTGAACGTTCGTTGCTTCCTATTGATATAATAGAAGAAATATGTTGTAGGATTCCAGTCGAATATTTGACGCAATTCAAATTGACGTGTAAACAATGGTTTGCTCTCTTGAAGGACAAGAGATTCATCTACAAATACTTGGATCTTTTTCAAGAACAAGAACGTTTCATAAGAATCGATCGTATTGTTCAGATCATCGATCCAGTGAAAGGAGCTCGTTCATCTTCACCAATCCCGCAAGAGTTCGACAATGTAGCTCAAATCTCCACTATGGTCCATTGTGATGGATTATTGTTATGTAGATGCAAGAACGAACGATCAAGAAGCTACAAACTCGCGGTTTGGAACCCGTTTTTGAGTCGAGTCAAATGGATCGAACCAATGGATTTTTATTCGAGTAATGATTTCTATGGTTTTGGATATGACAATGTATGTCGTGATGAGTACAAACTTCTAAGGATTTTCGATGGAGAGATTGAAGACGAGAGTGAAATCGCGGGGTCTTATGAACCGAAGATTCAGATATATGATTTCAAATCTGATTCATGGAGAATTGTTGATGATACTCGTGTGTCGGTGAAAGGAAACATGTATTGGATTGCACATTGGAATAATAGACCCGAAATTTTCATCCAGAGTTTTGATTTCTCCACAGAGACATTCAAAATCGTTTGCAATCTTCCATTCGAATGTAATGTATTAGACACGGCAGCCTTATCGAGTTTAAGAGGAGATAGGCTTTCTTTGTTACATCAAAGTGGAGAAACAATGAAGATCGAAGTTTGGATCACAAACAAATTGAGCGATGAGGTAGTCTCATGGACCAAGTACGTAGACGTGACTAGCCCTGATCTCCCGACATTACATACTGATCAACATCTCACTCATCCAAGTTATTTCATTGACAAGAACGATAATATCATGGTATGGTGTGAGCAAGAGACCGAAGAGGAAACCGATGATGATGTTTGCGTTAGCGTTTGCATGATTAGTAAAGATGGAATAGTGAAGAAACAAATTGATGCAGGACGATGTGATTTGTGTAGTGATAATAGGCCTTTTGTTTGCGGTTATGCATATGTTCCAAGTCTGGTTCCAGTTCCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

372

Amino Acids

43.39

Weight (kDa)

4.82

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 7 - 45 2.4e-06 F-box domain
FBA_1 PF07734 60 - 381 1e-88 F-box associated beta propeller domain
FBA_3 PF08268 89 - 308 8.1e-16 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 843
AccII CGCG 2 cut(s) 347, 506
AciI CCGC 4 cut(s) 245, 347, 506, 1077
AclI AACGTT 2 cut(s) 16, 172
AclWI GGATC 4 cut(s) 159, 203, 389, 803
AcsI RAATTY 1 cut(s) 633
AfaI GTAC 2 cut(s) 455, 839
AfiI CCNNNNNNNGG 1 cut(s) 218
AflIII ACRYGT 2 cut(s) 95, 597
AgsI TTSAA 6 cut(s) 88, 121, 161, 488, 544, 673
AjiI CACGTC 2 cut(s) 96, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 3 cut(s) 224, 266, 336
AluI AGCT 3 cut(s) 224, 266, 336
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 3 cut(s) 659, 829, 944
AlwI GGATC 4 cut(s) 159, 203, 389, 803
AoxI GGCC 1 cut(s) 1064
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 283, 831
AsuHPI GGTGA 2 cut(s) 228, 598
AsuII TTCGAA 1 cut(s) 696
AvaII GGWCC 2 cut(s) 283, 831
BaeI ACNNNNGTAYC 2 cut(s) 564, 597
BanII GRGCYC 1 cut(s) 226
BauI CACGAG 1 cut(s) 576
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 731
BccI CCATC 3 cut(s) 287, 470, 1001
BceAI ACGGC 1 cut(s) 732
BcgI CGANNNNNNTGC 2 cut(s) 708, 742
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 3 cut(s) 659, 829, 944
BfaI CTAG 1 cut(s) 852
BisI GCNGC 1 cut(s) 720
BlsI GCNGC 1 cut(s) 721
Bme18I GGWCC 2 cut(s) 283, 831
BmgBI CACGTC 2 cut(s) 96, 847
BmgT120I GGNCC 2 cut(s) 283, 831
BmiI GGNNCC 2 cut(s) 356, 1104
BmsI GCATC 2 cut(s) 302, 1021
BpiI GAAGAC 1 cut(s) 495
BplI GAGNNNNNCTC 2 cut(s) 809, 841
Bpu14I TTCGAA 1 cut(s) 696
Bsa29I ATCGAT 2 cut(s) 186, 207
BsaAI YACGTR 1 cut(s) 841
BsaBI GATNNNNATC 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 944
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 65, 212, 1106
Bse8I GATNNNNATC 1 cut(s) 885
BseCI ATCGAT 2 cut(s) 186, 207
BseGI GGATG 2 cut(s) 639, 895
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 1 cut(s) 218
BseNI ACTGG 3 cut(s) 65, 212, 1106
BseRI GAGGAG 1 cut(s) 753
BseXI GCAGC 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 347, 506
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 1 cut(s) 1066
BshVI ATCGAT 2 cut(s) 186, 207
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 3 cut(s) 659, 829, 944
BsnI GGCC 1 cut(s) 1066
Bso31I GGTCTC 1 cut(s) 944
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 226
BspACI CCGC 4 cut(s) 245, 347, 506, 1077
BspANI GGCC 1 cut(s) 1066
BspDI ATCGAT 2 cut(s) 186, 207
BspFNI CGCG 2 cut(s) 347, 506
BspLI GGNNCC 2 cut(s) 356, 1104
BspPI GGATC 4 cut(s) 159, 203, 389, 803
BspT104I TTCGAA 1 cut(s) 696
BspTNI GGTCTC 1 cut(s) 944
BsrI ACTGG 3 cut(s) 65, 212, 1106
BssSI CACGAG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 576
Bst6I CTCTTC 1 cut(s) 951
BstBAI YACGTR 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 696
BstDEI CTNAG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 639, 895
BstFNI CGCG 2 cut(s) 347, 506
BstMAI GTCTC 3 cut(s) 659, 829, 944
BstMCI CGRYCG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 601
BstSNI TACGTA 1 cut(s) 841
BstUI CGCG 2 cut(s) 347, 506
BstV1I GCAGC 1 cut(s) 731
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 2 cut(s) 186, 207
BsuRI GGCC 1 cut(s) 1066
BsuTUI ATCGAT 2 cut(s) 186, 207
BtgZI GCGATG 1 cut(s) 828
BtrI CACGTC 2 cut(s) 96, 847
BtsCI GGATG 2 cut(s) 639, 895
BtsIMutI CAGTG 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 283, 831
ClaI ATCGAT 2 cut(s) 186, 207
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 454, 838
CviAII CATG 6 cut(s) 9, 555, 598, 828, 931, 994
CviJI RGCY 8 cut(s) 5, 224, 266, 336, 722, 748, 855, 1066
CviKI_1 RGCY 8 cut(s) 5, 224, 266, 336, 722, 748, 855, 1066
CviQI GTAC 2 cut(s) 454, 838
DdeI CTNAG 1 cut(s) 464
Eam1104I CTCTTC 1 cut(s) 951
EarI CTCTTC 1 cut(s) 951
Ecl136II GAGCTC 1 cut(s) 224
Eco105I TACGTA 1 cut(s) 841
Eco147I AGGCCT 1 cut(s) 1066
Eco24I GRGCYC 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 944
Eco47I GGWCC 2 cut(s) 283, 831
Eco53kI GAGCTC 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 224
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 1 cut(s) 226
FaeI CATG 6 cut(s) 12, 558, 601, 831, 934, 997
FatI CATG 6 cut(s) 8, 554, 597, 827, 930, 993
FauI CCCGC 2 cut(s) 252, 499
FauNDI CATATG 1 cut(s) 1087
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 1 cut(s) 720
FokI GGATG 2 cut(s) 626, 882
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 1 cut(s) 720
FspBI CTAG 1 cut(s) 852
GluI GCNGC 1 cut(s) 720
HaeIII GGCC 1 cut(s) 1066
HgaI GACGC 1 cut(s) 88
Hin1II CATG 6 cut(s) 12, 558, 601, 831, 934, 997
HinfI GANTC 7 cut(s) 61, 132, 183, 367, 372, 526, 551
HphI GGTGA 2 cut(s) 228, 598
Hpy166II GTNNAC 3 cut(s) 14, 101, 844
Hpy188I TCNGA 3 cut(s) 201, 531, 550
Hpy188III TCNNGA 7 cut(s) 118, 161, 330, 446, 643, 865, 1112
Hpy8I GTNNAC 3 cut(s) 14, 101, 844
HpyAV CCTTC 1 cut(s) 115
HpyCH4IV ACGT 5 cut(s) 16, 95, 172, 840, 846
HpyCH4V TGCA 6 cut(s) 315, 611, 684, 993, 1034, 1085
HpyF3I CTNAG 1 cut(s) 464
HpySE526I ACGT 5 cut(s) 16, 95, 172, 840, 846
Hsp92II CATG 6 cut(s) 12, 558, 601, 831, 934, 997
Ksp22I TGATCA 1 cut(s) 880
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 78, 225, 656, 870, 1020, 1085
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 2 cut(s) 302, 1021
MaeI CTAG 1 cut(s) 852
MaeII ACGT 5 cut(s) 16, 95, 172, 840, 846
MaeIII GTNAC 2 cut(s) 756, 847
MboII GAAGA 8 cut(s) 56, 225, 500, 535, 680, 793, 968, 1030
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 6 cut(s) 83, 89, 561, 633, 806, 1026
MlyI GAGTC 2 cut(s) 376, 381
MnlI CCTC 3 cut(s) 731, 811, 952
Mph1103I ATGCAT 1 cut(s) 1087
MseI TTAA 1 cut(s) 734
MslI CAYNNNNRTG 3 cut(s) 291, 697, 765
MvnI CGCG 2 cut(s) 347, 506
NdeI CATATG 1 cut(s) 1087
NlaIII CATG 6 cut(s) 12, 558, 601, 831, 934, 997
NlaIV GGNNCC 2 cut(s) 356, 1104
NmuCI GTSAC 1 cut(s) 847
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 1 cut(s) 601
NspV TTCGAA 1 cut(s) 696
PceI AGGCCT 1 cut(s) 1066
PciI ACATGT 1 cut(s) 597
PfeI GAWTC 5 cut(s) 61, 132, 183, 526, 551
PkrI GCNGC 1 cut(s) 721
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 2 cut(s) 375, 380
PpsI GAGTC 2 cut(s) 375, 380
Ppu21I YACGTR 1 cut(s) 841
PscI ACATGT 1 cut(s) 597
Psp124BI GAGCTC 1 cut(s) 226
Psp1406I AACGTT 2 cut(s) 16, 172
PspN4I GGNNCC 2 cut(s) 356, 1104
PspPI GGNCC 2 cut(s) 283, 831
PsuI RGATCY 1 cut(s) 151
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 2 cut(s) 455, 839
RsaNI GTAC 2 cut(s) 454, 838
RseI CAYNNNNRTG 3 cut(s) 291, 697, 765
SacI GAGCTC 1 cut(s) 226
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 1 cut(s) 720
Sau96I GGNCC 2 cut(s) 283, 831
SchI GAGTC 2 cut(s) 376, 381
SduI GDGCHC 1 cut(s) 226
SetI ASST 9 cut(s) 19, 98, 175, 226, 268, 338, 822, 843, 849
SfaNI GCATC 2 cut(s) 302, 1021
SfuI TTCGAA 1 cut(s) 696
SinI GGWCC 2 cut(s) 283, 831
SmiMI CAYNNNNRTG 3 cut(s) 291, 697, 765
SnaBI TACGTA 1 cut(s) 841
Sse9I AATT 6 cut(s) 83, 89, 561, 633, 806, 1026
SseBI AGGCCT 1 cut(s) 1066
SsiI CCGC 4 cut(s) 245, 347, 506, 1077
SspI AATATT 1 cut(s) 74
SspMI CTAG 1 cut(s) 852
SstI GAGCTC 1 cut(s) 226
StuI AGGCCT 1 cut(s) 1066
TaiI ACGT 5 cut(s) 19, 98, 175, 843, 849
TaqII GACCGA 1 cut(s) 968
TasI AATT 6 cut(s) 83, 89, 561, 633, 806, 1026
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 5 cut(s) 61, 132, 183, 526, 551
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 1 cut(s) 847
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 1 cut(s) 847
TspDTI ATGAA 8 cut(s) 124, 166, 219, 531, 543, 628, 794, 898
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 283, 831
XapI RAATTY 1 cut(s) 633
XceI RCATGY 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 1 cut(s) 852
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.