3-phosphoinositide-dependent protein kinase activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 846 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 819 |
| AatII | GACGTC | 1 cut(s) | 817 |
| AccI | GTMKAC | 1 cut(s) | 817 |
| AccII | CGCG | 2 cut(s) | 171, 903 |
| AciI | CCGC | 2 cut(s) | 887, 1040 |
| AclWI | GGATC | 2 cut(s) | 543, 556 |
| AcoI | YGGCCR | 1 cut(s) | 1172 |
| AcsI | RAATTY | 3 cut(s) | 339, 404, 1049 |
| AcuI | CTGAAG | 1 cut(s) | 209 |
| AcyI | GRCGYC | 1 cut(s) | 814 |
| AfaI | GTAC | 1 cut(s) | 493 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 494 |
| AflIII | ACRYGT | 1 cut(s) | 359 |
| AgsI | TTSAA | 5 cut(s) | 232, 398, 515, 721, 1208 |
| AjnI | CCWGG | 1 cut(s) | 139 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 463, 495 |
| AluBI | AGCT | 2 cut(s) | 579, 594 |
| AluI | AGCT | 2 cut(s) | 579, 594 |
| Alw21I | GWGCWC | 1 cut(s) | 55 |
| Alw26I | GTCTC | 2 cut(s) | 827, 944 |
| AlwI | GGATC | 2 cut(s) | 543, 556 |
| AoxI | GGCC | 2 cut(s) | 354, 1172 |
| ApoI | RAATTY | 3 cut(s) | 339, 404, 1049 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1049 |
| AspS9I | GGNCC | 1 cut(s) | 133 |
| AsuHPI | GGTGA | 2 cut(s) | 943, 959 |
| AvaII | GGWCC | 1 cut(s) | 133 |
| BaeGI | GKGCMC | 1 cut(s) | 839 |
| BalI | TGGCCA | 1 cut(s) | 1174 |
| BamHI | GGATCC | 1 cut(s) | 548 |
| BauI | CACGAG | 1 cut(s) | 675 |
| Bbv12I | GWGCWC | 1 cut(s) | 55 |
| BccI | CCATC | 1 cut(s) | 352 |
| BceAI | ACGGC | 1 cut(s) | 451 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 189, 223 |
| BciT130I | CCWGG | 1 cut(s) | 141 |
| BciVI | GTATCC | 1 cut(s) | 1168 |
| BcoDI | GTCTC | 2 cut(s) | 827, 944 |
| BfaI | CTAG | 6 cut(s) | 6, 306, 390, 468, 504, 779 |
| BfmI | CTRYAG | 2 cut(s) | 348, 595 |
| BfuI | GTATCC | 1 cut(s) | 1168 |
| Bme1390I | CCNGG | 1 cut(s) | 141 |
| Bme18I | GGWCC | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 1 cut(s) | 133 |
| BmiI | GGNNCC | 2 cut(s) | 485, 550 |
| BmrFI | CCNGG | 1 cut(s) | 141 |
| BmsI | GCATC | 2 cut(s) | 693, 1027 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 629, 661 |
| BpuEI | CTTGAG | 1 cut(s) | 402 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1208 |
| BsaHI | GRCGYC | 1 cut(s) | 814 |
| BsaI | GGTCTC | 1 cut(s) | 944 |
| BsaJI | CCNNGG | 1 cut(s) | 334 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 494 |
| Bse1I | ACTGG | 2 cut(s) | 499, 740 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1208 |
| BseBI | CCWGG | 1 cut(s) | 141 |
| BseDI | CCNNGG | 1 cut(s) | 334 |
| BseGI | GGATG | 2 cut(s) | 684, 1090 |
| BseJI | GATNNNNATC | 1 cut(s) | 1208 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 494 |
| BseMII | CTCAG | 1 cut(s) | 781 |
| BseNI | ACTGG | 2 cut(s) | 499, 740 |
| BseRI | GAGGAG | 1 cut(s) | 170 |
| BseSI | GKGCMC | 1 cut(s) | 839 |
| Bsh1236I | CGCG | 2 cut(s) | 171, 903 |
| BshFI | GGCC | 2 cut(s) | 356, 1174 |
| BsiHKAI | GWGCWC | 1 cut(s) | 55 |
| BslFI | GGGAC | 1 cut(s) | 738 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 494 |
| BsmAI | GTCTC | 2 cut(s) | 827, 944 |
| BsmBI | CGTCTC | 1 cut(s) | 827 |
| BsmFI | GGGAC | 1 cut(s) | 738 |
| BsnI | GGCC | 2 cut(s) | 356, 1174 |
| Bso31I | GGTCTC | 1 cut(s) | 944 |
| Bsp1286I | GDGCHC | 2 cut(s) | 55, 839 |
| Bsp143I | GATC | 2 cut(s) | 200, 548 |
| Bsp19I | CCATGG | 1 cut(s) | 334 |
| BspACI | CCGC | 2 cut(s) | 887, 1040 |
| BspANI | GGCC | 2 cut(s) | 356, 1174 |
| BspCNI | CTCAG | 1 cut(s) | 780 |
| BspFNI | CGCG | 2 cut(s) | 171, 903 |
| BspLI | GGNNCC | 2 cut(s) | 485, 550 |
| BspPI | GGATC | 2 cut(s) | 543, 556 |
| BspTNI | GGTCTC | 1 cut(s) | 944 |
| BsrI | ACTGG | 2 cut(s) | 499, 740 |
| BssECI | CCNNGG | 1 cut(s) | 334 |
| BssMI | GATC | 2 cut(s) | 200, 548 |
| BssNI | GRCGYC | 1 cut(s) | 814 |
| BssSI | CACGAG | 1 cut(s) | 675 |
| BssT1I | CCWWGG | 1 cut(s) | 334 |
| Bst2BI | CACGAG | 1 cut(s) | 675 |
| Bst2UI | CCWGG | 1 cut(s) | 141 |
| Bst4CI | ACNGT | 3 cut(s) | 431, 572, 710 |
| Bst6I | CTCTTC | 1 cut(s) | 870 |
| BstACI | GRCGYC | 1 cut(s) | 814 |
| BstDEI | CTNAG | 3 cut(s) | 368, 767, 979 |
| BstDSI | CCRYGG | 1 cut(s) | 334 |
| BstF5I | GGATG | 2 cut(s) | 684, 1090 |
| BstFNI | CGCG | 2 cut(s) | 171, 903 |
| BstKTI | GATC | 2 cut(s) | 203, 551 |
| BstMAI | GTCTC | 2 cut(s) | 827, 944 |
| BstMBI | GATC | 2 cut(s) | 200, 548 |
| BstNI | CCWGG | 1 cut(s) | 141 |
| BstNSI | RCATGY | 1 cut(s) | 363 |
| BstSCI | CCNGG | 1 cut(s) | 139 |
| BstSFI | CTRYAG | 2 cut(s) | 348, 595 |
| BstSLI | GKGCMC | 1 cut(s) | 839 |
| BstUI | CGCG | 2 cut(s) | 171, 903 |
| BstX2I | RGATCY | 1 cut(s) | 548 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1105 |
| BstYI | RGATCY | 1 cut(s) | 548 |
| BsuI | GTATCC | 1 cut(s) | 1168 |
| BsuRI | GGCC | 2 cut(s) | 356, 1174 |
| BtgI | CCRYGG | 1 cut(s) | 334 |
| BtsCI | GGATG | 2 cut(s) | 684, 1090 |
| BtsI | GCAGTG | 2 cut(s) | 849, 990 |
| BtsIMutI | CAGTG | 4 cut(s) | 427, 839, 849, 990 |
| Cfr13I | GGNCC | 1 cut(s) | 133 |
| CseI | GACGC | 3 cut(s) | 464, 732, 843 |
| Csp6I | GTAC | 1 cut(s) | 492 |
| CviAII | CATG | 5 cut(s) | 292, 335, 360, 654, 973 |
| CviJI | RGCY | 9 cut(s) | 96, 269, 356, 372, 438, 579, 594, 609, 1174 |
| CviKI_1 | RGCY | 9 cut(s) | 96, 269, 356, 372, 438, 579, 594, 609, 1174 |
| CviQI | GTAC | 1 cut(s) | 492 |
| DdeI | CTNAG | 3 cut(s) | 368, 767, 979 |
| DpnI | GATC | 2 cut(s) | 202, 550 |
| DpnII | GATC | 2 cut(s) | 200, 548 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 819 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 819 |
| EaeI | YGGCCR | 1 cut(s) | 1172 |
| Eam1104I | CTCTTC | 1 cut(s) | 870 |
| EarI | CTCTTC | 1 cut(s) | 870 |
| Eco130I | CCWWGG | 1 cut(s) | 334 |
| Eco31I | GGTCTC | 1 cut(s) | 944 |
| Eco47I | GGWCC | 1 cut(s) | 133 |
| Eco57I | CTGAAG | 1 cut(s) | 209 |
| EcoRI | GAATTC | 1 cut(s) | 339 |
| EcoRII | CCWGG | 1 cut(s) | 139 |
| EcoT14I | CCWWGG | 1 cut(s) | 334 |
| ErhI | CCWWGG | 1 cut(s) | 334 |
| Esp3I | CGTCTC | 1 cut(s) | 827 |
| FaeI | CATG | 5 cut(s) | 295, 338, 363, 657, 976 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 867, 899 |
| FaqI | GGGAC | 1 cut(s) | 738 |
| FatI | CATG | 5 cut(s) | 291, 334, 359, 653, 972 |
| FblI | GTMKAC | 1 cut(s) | 817 |
| FokI | GGATG | 2 cut(s) | 671, 1097 |
| FspBI | CTAG | 6 cut(s) | 6, 306, 390, 468, 504, 779 |
| HaeIII | GGCC | 2 cut(s) | 356, 1174 |
| HgaI | GACGC | 3 cut(s) | 464, 732, 843 |
| Hin1I | GRCGYC | 1 cut(s) | 814 |
| Hin1II | CATG | 5 cut(s) | 295, 338, 363, 657, 976 |
| HinfI | GANTC | 8 cut(s) | 243, 286, 302, 380, 471, 1163, 1204, 1242 |
| HphI | GGTGA | 2 cut(s) | 943, 959 |
| Hpy166II | GTNNAC | 4 cut(s) | 365, 492, 818, 1111 |
| Hpy188I | TCNGA | 9 cut(s) | 62, 133, 189, 285, 748, 1000, 1079, 1154, 1162 |
| Hpy188III | TCNNGA | 3 cut(s) | 409, 665, 677 |
| Hpy8I | GTNNAC | 4 cut(s) | 365, 492, 818, 1111 |
| Hpy99I | CGWCG | 2 cut(s) | 458, 1058 |
| HpyAV | CCTTC | 3 cut(s) | 146, 715, 1211 |
| HpyCH4III | ACNGT | 3 cut(s) | 431, 572, 710 |
| HpyCH4IV | ACGT | 2 cut(s) | 814, 820 |
| HpyCH4V | TGCA | 3 cut(s) | 210, 450, 684 |
| HpyF3I | CTNAG | 3 cut(s) | 368, 767, 979 |
| HpySE526I | ACGT | 2 cut(s) | 814, 820 |
| Hsp92I | GRCGYC | 1 cut(s) | 814 |
| Hsp92II | CATG | 5 cut(s) | 295, 338, 363, 657, 976 |
| Kzo9I | GATC | 2 cut(s) | 200, 548 |
| LmnI | GCTCC | 2 cut(s) | 62, 969 |
| LpnPI | CCDG | 9 cut(s) | 113, 126, 153, 189, 480, 721, 852, 1002, 1198 |
| LweI | GCATC | 2 cut(s) | 693, 1027 |
| MaeI | CTAG | 6 cut(s) | 6, 306, 390, 468, 504, 779 |
| MaeII | ACGT | 2 cut(s) | 814, 820 |
| MaeIII | GTNAC | 6 cut(s) | 195, 431, 670, 677, 894, 931 |
| MalI | GATC | 2 cut(s) | 202, 550 |
| MboI | GATC | 2 cut(s) | 200, 548 |
| MboII | GAAGA | 2 cut(s) | 410, 887 |
| MflI | RGATCY | 1 cut(s) | 548 |
| MhlI | GDGCHC | 2 cut(s) | 55, 839 |
| MlsI | TGGCCA | 1 cut(s) | 1174 |
| MluCI | AATT | 8 cut(s) | 81, 223, 339, 404, 1049, 1069, 1147, 1186 |
| MluNI | TGGCCA | 1 cut(s) | 1174 |
| MlyI | GAGTC | 3 cut(s) | 296, 374, 1251 |
| MmeI | TCCRAC | 3 cut(s) | 40, 111, 1099 |
| MnlI | CCTC | 6 cut(s) | 148, 346, 651, 698, 1075, 1220 |
| Mox20I | TGGCCA | 1 cut(s) | 1174 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1049 |
| MscI | TGGCCA | 1 cut(s) | 1174 |
| MseI | TTAA | 5 cut(s) | 41, 84, 642, 1146, 1185 |
| MslI | CAYNNNNRTG | 1 cut(s) | 122 |
| Msp20I | TGGCCA | 1 cut(s) | 1174 |
| MspR9I | CCNGG | 1 cut(s) | 141 |
| MvaI | CCWGG | 1 cut(s) | 141 |
| MvnI | CGCG | 2 cut(s) | 171, 903 |
| NcoI | CCATGG | 1 cut(s) | 334 |
| NdeII | GATC | 2 cut(s) | 200, 548 |
| NlaIII | CATG | 5 cut(s) | 295, 338, 363, 657, 976 |
| NlaIV | GGNNCC | 2 cut(s) | 485, 550 |
| NmuCI | GTSAC | 4 cut(s) | 431, 670, 677, 931 |
| NspI | RCATGY | 1 cut(s) | 363 |
| PciI | ACATGT | 1 cut(s) | 359 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1158 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1049 |
| PfeI | GAWTC | 5 cut(s) | 243, 286, 471, 1163, 1204 |
| PfoI | TCCNGGA | 1 cut(s) | 139 |
| PleI | GAGTC | 3 cut(s) | 296, 374, 1250 |
| PpsI | GAGTC | 3 cut(s) | 296, 374, 1250 |
| PscI | ACATGT | 1 cut(s) | 359 |
| PsiI | TTATAA | 1 cut(s) | 846 |
| Psp6I | CCWGG | 1 cut(s) | 139 |
| PspGI | CCWGG | 1 cut(s) | 139 |
| PspN4I | GGNNCC | 2 cut(s) | 485, 550 |
| PspPI | GGNCC | 1 cut(s) | 133 |
| PsuI | RGATCY | 1 cut(s) | 548 |
| RsaI | GTAC | 1 cut(s) | 493 |
| RsaNI | GTAC | 1 cut(s) | 492 |
| RseI | CAYNNNNRTG | 1 cut(s) | 122 |
| SaqAI | TTAA | 5 cut(s) | 41, 84, 642, 1146, 1185 |
| Sau3AI | GATC | 2 cut(s) | 200, 548 |
| Sau96I | GGNCC | 1 cut(s) | 133 |
| SchI | GAGTC | 3 cut(s) | 296, 374, 1251 |
| ScrFI | CCNGG | 1 cut(s) | 141 |
| SduI | GDGCHC | 2 cut(s) | 55, 839 |
| SetI | ASST | 9 cut(s) | 102, 138, 509, 581, 596, 662, 817, 823, 1222 |
| SfaNI | GCATC | 2 cut(s) | 693, 1027 |
| SfcI | CTRYAG | 2 cut(s) | 348, 595 |
| SinI | GGWCC | 1 cut(s) | 133 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 122 |
| SmlI | CTYRAG | 1 cut(s) | 417 |
| SmoI | CTYRAG | 1 cut(s) | 417 |
| Sse9I | AATT | 8 cut(s) | 81, 223, 339, 404, 1049, 1069, 1147, 1186 |
| SsiI | CCGC | 2 cut(s) | 887, 1040 |
| SspI | AATATT | 2 cut(s) | 39, 588 |
| SspMI | CTAG | 6 cut(s) | 6, 306, 390, 468, 504, 779 |
| StyD4I | CCNGG | 1 cut(s) | 139 |
| StyI | CCWWGG | 1 cut(s) | 334 |
| TaaI | ACNGT | 3 cut(s) | 431, 572, 710 |
| TaiI | ACGT | 2 cut(s) | 817, 823 |
| TaqI | TCGA | 6 cut(s) | 317, 378, 408, 690, 1035, 1245 |
| TasI | AATT | 8 cut(s) | 81, 223, 339, 404, 1049, 1069, 1147, 1186 |
| TatI | WGTACW | 1 cut(s) | 491 |
| TfiI | GAWTC | 5 cut(s) | 243, 286, 471, 1163, 1204 |
| Tru1I | TTAA | 5 cut(s) | 41, 84, 642, 1146, 1185 |
| Tru9I | TTAA | 5 cut(s) | 41, 84, 642, 1146, 1185 |
| TscAI | CASTG | 4 cut(s) | 434, 839, 856, 990 |
| TseFI | GTSAC | 4 cut(s) | 431, 670, 677, 931 |
| Tsp45I | GTSAC | 4 cut(s) | 431, 670, 677, 931 |
| TspDTI | ATGAA | 2 cut(s) | 997, 1101 |
| TspGWI | ACGGA | 1 cut(s) | 853 |
| TspRI | CASTG | 4 cut(s) | 434, 839, 856, 990 |
| VpaK11BI | GGWCC | 1 cut(s) | 133 |
| XapI | RAATTY | 3 cut(s) | 339, 404, 1049 |
| XceI | RCATGY | 1 cut(s) | 363 |
| XmiI | GTMKAC | 1 cut(s) | 817 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1049 |
| XspI | CTAG | 6 cut(s) | 6, 306, 390, 468, 504, 779 |
| ZraI | GACGTC | 1 cut(s) | 815 |