AT3G22710

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Reverse (-)
8026202 .. 8027274
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22710.1

Sequence Viewer

Length: 981 bp
ATGACGATGCCCGATCTTCCGCCAGATTTGGTCGAGGAAATACTTTCTAGGGTTCCGGCGACATCTGTGAAAAAGTTACGATCTACTTGCACACAATGGAACGCTATATTCAAAGATGAAAGATTCACTGAGAAGCACTTTAGTAAAGCACCAAAGGAGTCTATGGTTCTCATGTTGAAGGAGCATAGGGTTTGCCCGGACAAAAGACTCGTGGTTTGGAATCCGTGTTTGGGGGAAACCAAGTGGATCCAACTCAAAGTTGATTACCGGAGATACGTCTCCAAGTTTTGTCTAGGGTATATCCAAAACAACGAATCTCGCCGTAGCTATAAAATCTTGCGGAGTTGGTATAGTTACGATGACAAATCATCACCGCGCCAAAGAGATTTGGGGTTTGAAATATACGAGTTTATCTCTGATTCGTCATGGAGAGTTCTTAATGACGGAAATACTTACTTGCTTGCTTATGATGTTGAAGAAAATTCTAGAGTTGTACTAATGTTTGATTTTACAACAGAGAGATTCAAACGTCTACGCCTTCCGCATTTCCAAGATGTTGGTAATATGGATCTATCAGTTGTTAGAGAAGAACAACTCTCAATATTACATTGGACTCGTAATACATCAAAGATGGAGATTTGGATAACTAATAACATTGATACTGATGCTACATTGTTGTGGAGACTGCACTTACACACTCAAGTCTTCTCACGTAATTGTGTTCGAGTCTTCTCAAGTCTATATATCGACAAGGAGAAGAAAGTTGTCTTGTGTTGTAATGTTAATGATGATGCGACCAGCAAGAACATAGTATACATTATCGGAGAGGACAATGGATATTACACAGAAATCCTTTTCCTACTCCCTATAAACATACATTGGGTTCTACTTGACCGTAATAAAAAATGGTATTCATCTATTTTCAATTATGTTCCGCGTTTGGTTCAAATCTATAATGGTCATCTCTTATTTTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

326

Amino Acids

38.94

Weight (kDa)

8.69

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 44 3.7e-09 F-box domain
FBA_1 PF07734 67 - 317 1.5e-56 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 532, 813
AccII CGCG 2 cut(s) 376, 937
AciI CCGC 5 cut(s) 20, 340, 374, 542, 935
AclWI GGATC 3 cut(s) 241, 254, 576
AcsI RAATTY 1 cut(s) 481
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 7 cut(s) 112, 178, 398, 476, 526, 925, 947
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 283, 676
AlwI GGATC 3 cut(s) 241, 254, 576
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AspLEI GCGC 1 cut(s) 378
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 363
BamHI GGATCC 1 cut(s) 246
BauI CACGAG 1 cut(s) 209
BbsI GAAGAC 2 cut(s) 697, 721
BccI CCATC 1 cut(s) 625
BceAI ACGGC 1 cut(s) 306
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 283, 676
BfaI CTAG 3 cut(s) 48, 293, 486
Bme1390I CCNGG 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 54, 248
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 2 cut(s) 655, 781
BpiI GAAGAC 2 cut(s) 697, 721
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpuEI CTTGAG 2 cut(s) 684, 718
BpuMI CCSGG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 713
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 230
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 2 cut(s) 376, 937
BsiSI CCGG 3 cut(s) 56, 197, 268
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 2 cut(s) 283, 676
BsmBI CGTCTC 1 cut(s) 283
Bsp143I GATC 4 cut(s) 13, 80, 246, 568
BspACI CCGC 5 cut(s) 20, 340, 374, 542, 935
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 2 cut(s) 376, 937
BspLI GGNNCC 2 cut(s) 54, 248
BspPI GGATC 3 cut(s) 241, 254, 576
BssMI GATC 4 cut(s) 13, 80, 246, 568
BssNAI GTATAC 1 cut(s) 814
BssSI CACGAG 1 cut(s) 209
Bst1107I GTATAC 1 cut(s) 814
Bst2BI CACGAG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 896
BstBAI YACGTR 1 cut(s) 713
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 1 cut(s) 129
BstFNI CGCG 2 cut(s) 376, 937
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 4 cut(s) 16, 83, 249, 571
BstMAI GTCTC 2 cut(s) 283, 676
BstMBI GATC 4 cut(s) 13, 80, 246, 568
BstSCI CCNGG 1 cut(s) 195
BstUI CGCG 2 cut(s) 376, 937
BstV2I GAAGAC 2 cut(s) 697, 721
BstX2I RGATCY 2 cut(s) 246, 568
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 2 cut(s) 246, 568
BstZ17I GTATAC 1 cut(s) 814
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 462
CfoI GCGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 494
CviAII CATG 2 cut(s) 172, 426
CviJI RGCY 1 cut(s) 327
CviKI_1 RGCY 1 cut(s) 327
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 1 cut(s) 129
DpnI GATC 4 cut(s) 15, 82, 248, 570
DpnII GATC 4 cut(s) 13, 80, 246, 568
EciI GGCGGA 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 2 cut(s) 175, 429
FatI CATG 2 cut(s) 171, 425
FblI GTMKAC 2 cut(s) 532, 813
FspBI CTAG 3 cut(s) 48, 293, 486
GlaI GCGC 1 cut(s) 377
HapII CCGG 3 cut(s) 56, 197, 268
HhaI GCGC 1 cut(s) 378
Hin1II CATG 2 cut(s) 175, 429
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 9 cut(s) 123, 158, 207, 220, 314, 419, 522, 613, 726
HpaII CCGG 3 cut(s) 56, 197, 268
HphI GGTGA 1 cut(s) 363
Hpy166II GTNNAC 2 cut(s) 533, 814
Hpy188I TCNGA 2 cut(s) 418, 824
Hpy188III TCNNGA 1 cut(s) 486
Hpy8I GTNNAC 2 cut(s) 533, 814
HpyAV CCTTC 2 cut(s) 172, 548
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 3 cut(s) 276, 529, 712
HpyCH4V TGCA 2 cut(s) 90, 688
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 3 cut(s) 276, 529, 712
Hsp92II CATG 2 cut(s) 175, 429
HspAI GCGC 1 cut(s) 376
Kzo9I GATC 4 cut(s) 13, 80, 246, 568
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 5 cut(s) 36, 69, 210, 281, 811
LweI GCATC 2 cut(s) 655, 781
MaeI CTAG 3 cut(s) 48, 293, 486
MaeII ACGT 3 cut(s) 276, 529, 712
MaeIII GTNAC 2 cut(s) 75, 353
MalI GATC 4 cut(s) 15, 82, 248, 570
MboI GATC 4 cut(s) 13, 80, 246, 568
MboII GAAGA 6 cut(s) 8, 488, 599, 697, 721, 769
MflI RGATCY 2 cut(s) 246, 568
MluCI AATT 3 cut(s) 481, 715, 925
MlyI GAGTC 4 cut(s) 167, 201, 607, 735
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 2 cut(s) 28, 820
MseI TTAA 3 cut(s) 438, 783, 979
MslI CAYNNNNRTG 1 cut(s) 676
MspI CCGG 3 cut(s) 56, 197, 268
MspR9I CCNGG 1 cut(s) 197
MvnI CGCG 2 cut(s) 376, 937
NciI CCSGG 1 cut(s) 197
NdeII GATC 4 cut(s) 13, 80, 246, 568
NlaIII CATG 2 cut(s) 175, 429
NlaIV GGNNCC 2 cut(s) 54, 248
PfeI GAWTC 5 cut(s) 123, 220, 314, 419, 522
PleI GAGTC 4 cut(s) 166, 201, 607, 734
PpsI GAGTC 4 cut(s) 166, 201, 607, 734
Ppu21I YACGTR 1 cut(s) 713
PspN4I GGNNCC 2 cut(s) 54, 248
PsrI GAACNNNNNNTAC 2 cut(s) 797, 829
PsuI RGATCY 2 cut(s) 246, 568
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 676
SaqAI TTAA 3 cut(s) 438, 783, 979
Sau3AI GATC 4 cut(s) 13, 80, 246, 568
SchI GAGTC 4 cut(s) 167, 201, 607, 735
ScrFI CCNGG 1 cut(s) 197
SetI ASST 4 cut(s) 279, 329, 532, 715
SfaNI GCATC 2 cut(s) 655, 781
SmiMI CAYNNNNRTG 1 cut(s) 676
SmlI CTYRAG 2 cut(s) 699, 733
SmoI CTYRAG 2 cut(s) 699, 733
Sse9I AATT 3 cut(s) 481, 715, 925
SsiI CCGC 5 cut(s) 20, 340, 374, 542, 935
SspI AATATT 1 cut(s) 603
SspMI CTAG 3 cut(s) 48, 293, 486
StyD4I CCNGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 3 cut(s) 279, 532, 715
TaqI TCGA 3 cut(s) 33, 724, 747
TasI AATT 3 cut(s) 481, 715, 925
TatI WGTACW 1 cut(s) 493
TfiI GAWTC 5 cut(s) 123, 220, 314, 419, 522
Tru1I TTAA 3 cut(s) 438, 783, 979
Tru9I TTAA 3 cut(s) 438, 783, 979
TscAI CASTG 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 132, 903
TspGWI ACGGA 2 cut(s) 213, 459
TspRI CASTG 1 cut(s) 133
XapI RAATTY 1 cut(s) 481
XbaI TCTAGA 1 cut(s) 485
XmiI GTMKAC 2 cut(s) 532, 813
XspI CTAG 3 cut(s) 48, 293, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.