Belongs to the AAA ATPase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 194 |
| AccII | CGCG | 1 cut(s) | 560 |
| AciI | CCGC | 5 cut(s) | 5, 558, 560, 574, 638 |
| AclI | AACGTT | 1 cut(s) | 213 |
| AclWI | GGATC | 2 cut(s) | 383, 845 |
| AcoI | YGGCCR | 1 cut(s) | 760 |
| AcsI | RAATTY | 1 cut(s) | 270 |
| AcuI | CTGAAG | 2 cut(s) | 188, 246 |
| AfaI | GTAC | 1 cut(s) | 442 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 359, 584 |
| AgsI | TTSAA | 3 cut(s) | 218, 511, 595 |
| AjiI | CACGTC | 1 cut(s) | 286 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 234, 266 |
| AluBI | AGCT | 3 cut(s) | 96, 134, 634 |
| AluI | AGCT | 3 cut(s) | 96, 134, 634 |
| Alw26I | GTCTC | 3 cut(s) | 374, 493, 540 |
| AlwI | GGATC | 2 cut(s) | 383, 845 |
| AoxI | GGCC | 2 cut(s) | 555, 760 |
| ApeKI | GCWGC | 3 cut(s) | 134, 631, 720 |
| ApoI | RAATTY | 1 cut(s) | 270 |
| AspA2I | CCTAGG | 1 cut(s) | 332 |
| AspLEI | GCGC | 1 cut(s) | 195 |
| AspS9I | GGNCC | 1 cut(s) | 555 |
| AsuC2I | CCSGG | 1 cut(s) | 370 |
| AsuHPI | GGTGA | 1 cut(s) | 748 |
| AvrII | CCTAGG | 1 cut(s) | 332 |
| BamHI | GGATCC | 1 cut(s) | 850 |
| BanII | GRGCYC | 1 cut(s) | 702 |
| BbsI | GAAGAC | 1 cut(s) | 52 |
| BbvI | GCAGC | 3 cut(s) | 121, 643, 732 |
| BccI | CCATC | 3 cut(s) | 163, 664, 841 |
| BceAI | ACGGC | 1 cut(s) | 747 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 42, 76 |
| BciVI | GTATCC | 2 cut(s) | 422, 652 |
| BcnI | CCSGG | 1 cut(s) | 370 |
| BcoDI | GTCTC | 3 cut(s) | 374, 493, 540 |
| BfaI | CTAG | 3 cut(s) | 21, 84, 333 |
| BfuI | GTATCC | 2 cut(s) | 422, 652 |
| BglII | AGATCT | 1 cut(s) | 675 |
| BisI | GCNGC | 5 cut(s) | 135, 558, 574, 632, 721 |
| BlnI | CCTAGG | 1 cut(s) | 332 |
| BlsI | GCNGC | 5 cut(s) | 136, 559, 575, 633, 722 |
| Bme1390I | CCNGG | 1 cut(s) | 370 |
| BmgBI | CACGTC | 1 cut(s) | 286 |
| BmgT120I | GGNCC | 1 cut(s) | 555 |
| BmiI | GGNNCC | 2 cut(s) | 27, 852 |
| BmrFI | CCNGG | 1 cut(s) | 370 |
| BmsI | GCATC | 4 cut(s) | 180, 324, 397, 778 |
| BpiI | GAAGAC | 1 cut(s) | 52 |
| BpuEI | CTTGAG | 1 cut(s) | 481 |
| BpuMI | CCSGG | 1 cut(s) | 370 |
| BsaBI | GATNNNNATC | 1 cut(s) | 674 |
| BsaJI | CCNNGG | 2 cut(s) | 332, 558 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 359, 584 |
| Bse118I | RCCGGY | 1 cut(s) | 758 |
| Bse8I | GATNNNNATC | 1 cut(s) | 674 |
| BseDI | CCNNGG | 2 cut(s) | 332, 558 |
| BseGI | GGATG | 1 cut(s) | 852 |
| BseJI | GATNNNNATC | 1 cut(s) | 674 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 359, 584 |
| BseMII | CTCAG | 2 cut(s) | 410, 495 |
| BseRI | GAGGAG | 3 cut(s) | 23, 481, 824 |
| BseX3I | CGGCCG | 1 cut(s) | 760 |
| BseXI | GCAGC | 3 cut(s) | 121, 643, 732 |
| Bsh1236I | CGCG | 1 cut(s) | 560 |
| Bsh1285I | CGRYCG | 1 cut(s) | 763 |
| BshFI | GGCC | 2 cut(s) | 557, 762 |
| BsiEI | CGRYCG | 1 cut(s) | 763 |
| BsiSI | CCGG | 2 cut(s) | 370, 759 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 359, 584 |
| BsmAI | GTCTC | 3 cut(s) | 374, 493, 540 |
| BsnI | GGCC | 2 cut(s) | 557, 762 |
| Bsp1286I | GDGCHC | 1 cut(s) | 702 |
| Bsp143I | GATC | 4 cut(s) | 265, 375, 675, 850 |
| BspACI | CCGC | 5 cut(s) | 5, 558, 560, 574, 638 |
| BspANI | GGCC | 2 cut(s) | 557, 762 |
| BspCNI | CTCAG | 2 cut(s) | 409, 496 |
| BspFNI | CGCG | 1 cut(s) | 560 |
| BspHI | TCATGA | 2 cut(s) | 162, 318 |
| BspLI | GGNNCC | 2 cut(s) | 27, 852 |
| BspPI | GGATC | 2 cut(s) | 383, 845 |
| BsrFI | RCCGGY | 1 cut(s) | 758 |
| BssAI | RCCGGY | 1 cut(s) | 758 |
| BssECI | CCNNGG | 2 cut(s) | 332, 558 |
| BssMI | GATC | 4 cut(s) | 265, 375, 675, 850 |
| BssT1I | CCWWGG | 1 cut(s) | 332 |
| Bst4CI | ACNGT | 2 cut(s) | 302, 709 |
| BstC8I | GCNNGC | 1 cut(s) | 636 |
| BstDEI | CTNAG | 4 cut(s) | 41, 396, 504, 690 |
| BstDSI | CCRYGG | 1 cut(s) | 558 |
| BstF5I | GGATG | 1 cut(s) | 852 |
| BstFNI | CGCG | 1 cut(s) | 560 |
| BstHHI | GCGC | 1 cut(s) | 195 |
| BstKTI | GATC | 4 cut(s) | 268, 378, 678, 853 |
| BstMAI | GTCTC | 3 cut(s) | 374, 493, 540 |
| BstMBI | GATC | 4 cut(s) | 265, 375, 675, 850 |
| BstMCI | CGRYCG | 1 cut(s) | 763 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 131, 573 |
| BstSCI | CCNGG | 1 cut(s) | 368 |
| BstUI | CGCG | 1 cut(s) | 560 |
| BstV1I | GCAGC | 3 cut(s) | 121, 643, 732 |
| BstV2I | GAAGAC | 1 cut(s) | 52 |
| BstX2I | RGATCY | 2 cut(s) | 675, 850 |
| BstYI | RGATCY | 2 cut(s) | 675, 850 |
| BstZI | CGGCCG | 1 cut(s) | 760 |
| BsuI | GTATCC | 2 cut(s) | 422, 652 |
| BsuRI | GGCC | 2 cut(s) | 557, 762 |
| BtgI | CCRYGG | 1 cut(s) | 558 |
| BtrI | CACGTC | 1 cut(s) | 286 |
| BtsCI | GGATG | 1 cut(s) | 852 |
| BtsI | GCAGTG | 2 cut(s) | 292, 831 |
| BtsIMutI | CAGTG | 3 cut(s) | 292, 744, 831 |
| Cac8I | GCNNGC | 1 cut(s) | 636 |
| CciI | TCATGA | 2 cut(s) | 162, 318 |
| CfoI | GCGC | 1 cut(s) | 195 |
| Cfr10I | RCCGGY | 1 cut(s) | 758 |
| Cfr13I | GGNCC | 1 cut(s) | 555 |
| Cfr42I | CCGCGG | 1 cut(s) | 561 |
| CseI | GACGC | 1 cut(s) | 266 |
| Csp6I | GTAC | 1 cut(s) | 441 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 353, 388 |
| CviAII | CATG | 4 cut(s) | 163, 319, 537, 714 |
| CviJI | RGCY | 7 cut(s) | 96, 134, 337, 557, 634, 700, 762 |
| CviKI_1 | RGCY | 7 cut(s) | 96, 134, 337, 557, 634, 700, 762 |
| CviQI | GTAC | 1 cut(s) | 441 |
| DdeI | CTNAG | 4 cut(s) | 41, 396, 504, 690 |
| DpnI | GATC | 4 cut(s) | 267, 377, 677, 852 |
| DpnII | GATC | 4 cut(s) | 265, 375, 675, 850 |
| EaeI | YGGCCR | 1 cut(s) | 760 |
| EagI | CGGCCG | 1 cut(s) | 760 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| EclXI | CGGCCG | 1 cut(s) | 760 |
| Eco130I | CCWWGG | 1 cut(s) | 332 |
| Eco24I | GRGCYC | 1 cut(s) | 702 |
| Eco52I | CGGCCG | 1 cut(s) | 760 |
| Eco57I | CTGAAG | 2 cut(s) | 188, 246 |
| EcoT14I | CCWWGG | 1 cut(s) | 332 |
| EcoT22I | ATGCAT | 2 cut(s) | 317, 412 |
| EcoT38I | GRGCYC | 1 cut(s) | 702 |
| ErhI | CCWWGG | 1 cut(s) | 332 |
| FaeI | CATG | 4 cut(s) | 166, 322, 540, 717 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 716, 748 |
| FatI | CATG | 4 cut(s) | 162, 318, 536, 713 |
| Fnu4HI | GCNGC | 5 cut(s) | 135, 558, 574, 632, 721 |
| FriOI | GRGCYC | 1 cut(s) | 702 |
| Fsp4HI | GCNGC | 5 cut(s) | 135, 558, 574, 632, 721 |
| FspAI | RTGCGCAY | 1 cut(s) | 194 |
| FspBI | CTAG | 3 cut(s) | 21, 84, 333 |
| FspI | TGCGCA | 1 cut(s) | 194 |
| GlaI | GCGC | 1 cut(s) | 194 |
| GluI | GCNGC | 5 cut(s) | 135, 558, 574, 632, 721 |
| HaeIII | GGCC | 2 cut(s) | 557, 762 |
| HapII | CCGG | 2 cut(s) | 370, 759 |
| HgaI | GACGC | 1 cut(s) | 266 |
| HhaI | GCGC | 1 cut(s) | 195 |
| Hin1II | CATG | 4 cut(s) | 166, 322, 540, 717 |
| Hin6I | GCGC | 1 cut(s) | 193 |
| HinP1I | GCGC | 1 cut(s) | 193 |
| HinfI | GANTC | 2 cut(s) | 48, 533 |
| HpaII | CCGG | 2 cut(s) | 370, 759 |
| HphI | GGTGA | 1 cut(s) | 748 |
| Hpy166II | GTNNAC | 3 cut(s) | 236, 242, 362 |
| Hpy188I | TCNGA | 7 cut(s) | 30, 53, 207, 265, 399, 428, 532 |
| Hpy188III | TCNNGA | 6 cut(s) | 84, 163, 319, 379, 460, 595 |
| Hpy8I | GTNNAC | 3 cut(s) | 236, 242, 362 |
| Hpy99I | CGWCG | 2 cut(s) | 260, 302 |
| HpyAV | CCTTC | 1 cut(s) | 578 |
| HpyCH4III | ACNGT | 2 cut(s) | 302, 709 |
| HpyCH4IV | ACGT | 4 cut(s) | 213, 238, 276, 285 |
| HpyCH4V | TGCA | 7 cut(s) | 63, 101, 137, 315, 410, 791, 824 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 131, 573 |
| HpyF3I | CTNAG | 4 cut(s) | 41, 396, 504, 690 |
| HpySE526I | ACGT | 4 cut(s) | 213, 238, 276, 285 |
| Hsp92II | CATG | 4 cut(s) | 166, 322, 540, 717 |
| HspAI | GCGC | 1 cut(s) | 193 |
| KspI | CCGCGG | 1 cut(s) | 561 |
| Kzo9I | GATC | 4 cut(s) | 265, 375, 675, 850 |
| LmnI | GCTCC | 2 cut(s) | 93, 717 |
| LpnPI | CCDG | 3 cut(s) | 383, 664, 772 |
| Lsp1109I | GCAGC | 3 cut(s) | 121, 643, 732 |
| LweI | GCATC | 4 cut(s) | 180, 324, 397, 778 |
| MaeI | CTAG | 3 cut(s) | 21, 84, 333 |
| MaeII | ACGT | 4 cut(s) | 213, 238, 276, 285 |
| MaeIII | GTNAC | 4 cut(s) | 281, 347, 682, 764 |
| MalI | GATC | 4 cut(s) | 267, 377, 677, 852 |
| MboI | GATC | 4 cut(s) | 265, 375, 675, 850 |
| MboII | GAAGA | 6 cut(s) | 57, 194, 583, 721, 790, 826 |
| MflI | RGATCY | 2 cut(s) | 675, 850 |
| MhlI | GDGCHC | 1 cut(s) | 702 |
| MluCI | AATT | 2 cut(s) | 218, 270 |
| MlyI | GAGTC | 2 cut(s) | 42, 527 |
| MmeI | TCCRAC | 1 cut(s) | 819 |
| MnlI | CCTC | 6 cut(s) | 4, 405, 502, 534, 802, 837 |
| Mph1103I | ATGCAT | 2 cut(s) | 317, 412 |
| MseI | TTAA | 1 cut(s) | 525 |
| MslI | CAYNNNNRTG | 1 cut(s) | 167 |
| MspA1I | CMGCKG | 1 cut(s) | 560 |
| MspI | CCGG | 2 cut(s) | 370, 759 |
| MspR9I | CCNGG | 1 cut(s) | 370 |
| MvnI | CGCG | 1 cut(s) | 560 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 131, 573 |
| NciI | CCSGG | 1 cut(s) | 370 |
| NdeII | GATC | 4 cut(s) | 265, 375, 675, 850 |
| NlaIII | CATG | 4 cut(s) | 166, 322, 540, 717 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 852 |
| NmuCI | GTSAC | 1 cut(s) | 281 |
| NsbI | TGCGCA | 1 cut(s) | 194 |
| NsiI | ATGCAT | 2 cut(s) | 317, 412 |
| PagI | TCATGA | 2 cut(s) | 162, 318 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 282 |
| PkrI | GCNGC | 5 cut(s) | 136, 559, 575, 633, 722 |
| PleI | GAGTC | 2 cut(s) | 42, 527 |
| PpsI | GAGTC | 2 cut(s) | 42, 527 |
| Psp1406I | AACGTT | 1 cut(s) | 213 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 852 |
| PspPI | GGNCC | 1 cut(s) | 555 |
| PsuI | RGATCY | 2 cut(s) | 675, 850 |
| RsaI | GTAC | 1 cut(s) | 442 |
| RsaNI | GTAC | 1 cut(s) | 441 |
| RseI | CAYNNNNRTG | 1 cut(s) | 167 |
| SacII | CCGCGG | 1 cut(s) | 561 |
| SaqAI | TTAA | 1 cut(s) | 525 |
| SatI | GCNGC | 5 cut(s) | 135, 558, 574, 632, 721 |
| Sau3AI | GATC | 4 cut(s) | 265, 375, 675, 850 |
| Sau96I | GGNCC | 1 cut(s) | 555 |
| SchI | GAGTC | 2 cut(s) | 42, 527 |
| ScrFI | CCNGG | 1 cut(s) | 370 |
| SduI | GDGCHC | 1 cut(s) | 702 |
| SfaNI | GCATC | 4 cut(s) | 180, 324, 397, 778 |
| Sfr303I | CCGCGG | 1 cut(s) | 561 |
| SgrBI | CCGCGG | 1 cut(s) | 561 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 167 |
| SmlI | CTYRAG | 1 cut(s) | 460 |
| SmoI | CTYRAG | 1 cut(s) | 460 |
| Sse9I | AATT | 2 cut(s) | 218, 270 |
| SsiI | CCGC | 5 cut(s) | 5, 558, 560, 574, 638 |
| SspMI | CTAG | 3 cut(s) | 21, 84, 333 |
| StyD4I | CCNGG | 1 cut(s) | 368 |
| StyI | CCWWGG | 1 cut(s) | 332 |
| TaaI | ACNGT | 2 cut(s) | 302, 709 |
| TaiI | ACGT | 4 cut(s) | 216, 241, 279, 288 |
| TaqI | TCGA | 1 cut(s) | 378 |
| TasI | AATT | 2 cut(s) | 218, 270 |
| TauI | GCSGC | 2 cut(s) | 560, 576 |
| Tru1I | TTAA | 1 cut(s) | 525 |
| Tru9I | TTAA | 1 cut(s) | 525 |
| TscAI | CASTG | 3 cut(s) | 299, 744, 831 |
| TseFI | GTSAC | 1 cut(s) | 281 |
| TseI | GCWGC | 3 cut(s) | 134, 631, 720 |
| Tsp45I | GTSAC | 1 cut(s) | 281 |
| TspDTI | ATGAA | 2 cut(s) | 335, 790 |
| TspGWI | ACGGA | 1 cut(s) | 269 |
| TspRI | CASTG | 3 cut(s) | 299, 744, 831 |
| XapI | RAATTY | 1 cut(s) | 270 |
| XbaI | TCTAGA | 1 cut(s) | 83 |
| XmaJI | CCTAGG | 1 cut(s) | 332 |
| XspI | CTAG | 3 cut(s) | 21, 84, 333 |
| Zsp2I | ATGCAT | 2 cut(s) | 317, 412 |