AT3G22700

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Reverse (-)
8024794 .. 8025906
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22700.1

Sequence Viewer

Length: 1020 bp
ATGATGTCGAATCTTCCTTTGGATTTGGTAGAGGAAATACTCTCTAGGGTTCCAGCCACATCTCTCAAACGATTACGATCGACTTGTAGACAATGGAACGCTTTATTGAAAGACCGAAGATTCACAGAGAAGCACTTTCGTAAAGCACCAAAGGAGTCTCTAGTTCTCATGTTGAAAGAAATAAGCGTCAATCTCAACGTTACTCCTCCATCTATAGAGTTTAAAGATGCACTTGGCTTAAAGGATTCCCATTCTAATTCAGAACAAGTTGATATAGTCCAAGTTCTTCACTGCGATGGTTTGTTGTTATGCACCACCAAGGACAATAGACACGTGGTTTGGAATCCGTGTTTGGGGGAAACCCATTGGATCCAATTCAAAGTTGATTACGGGAGAGTCTACTCCAGCTTTGCTCTAGGATATATCCAAAACAACGAATCTTGCCGTAGCTACAAAATCTTGTGGAGGTGGAAAAGTAACGATTACAAATCATCACCGCGTCAAAGAGGGTTTGAAATATACGAGTTTATCTCTGATAAATGGAGGGTTATTGATGACGTCAATCACGACTCTTTGGTCAATCACAACTATTTAGGACGTTGCTGTCGCGTGTCTTTGAAGGGAAATATTTACTGGCTTGTTGATGATGTTGAAGACAATTCTAGATCTTTACTTATGTTTGACTTTAAAACAGAGAGATTTAAACGTCTATGCCTTCCGCATTTCGAAAATGTTGGTCACATGGTTTTATCATTTGTTAGAGAAGAACAACTCTCAGTATTATATTGGAGCCGTGCTACACCAAAGATGGAGATATGGATAACCAATAACATTGATACTGATGCTACATTGTTGTGGAGACTGCACTTAGACACTCGCTTTAATTGTGTTCGAATCTTCTCAAGTCTCTATTTCGAGGAGGAGAAGAAAGTCGTCTTGTGTTGTAATGTTAAATTGTTAATGATGATAAGATCAGCAAGAACATGGTATACATTATCGGAGAGGACGATGGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

339

Amino Acids

40.21

Weight (kDa)

8.68

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 43 6.6e-11 F-box domain
F-box-like PF12937 2 - 41 2e-07 F-box-like
FBA_1 PF07734 60 - 317 4.4e-74 F-box associated beta propeller domain
FBA_3 PF08268 81 - 279 1.3e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 575, 603
AatII GACGTC 1 cut(s) 561
AccI GTMKAC 3 cut(s) 88, 399, 989
AccII CGCG 2 cut(s) 499, 609
AciI CCGC 2 cut(s) 497, 719
AclI AACGTT 1 cut(s) 198
AclWI GGATC 2 cut(s) 364, 377
AcvI CACGTG 1 cut(s) 334
AcyI GRCGYC 1 cut(s) 558
AfiI CCNNNNNNNGG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 6 cut(s) 109, 175, 379, 515, 619, 653
AjuI GAANNNNNNNTTGG 3 cut(s) 34, 335, 367
AluBI AGCT 2 cut(s) 408, 450
AluI AGCT 2 cut(s) 408, 450
Alw26I GTCTC 3 cut(s) 162, 853, 911
AlwI GGATC 2 cut(s) 364, 377
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AsuHPI GGTGA 1 cut(s) 486
AsuII TTCGAA 2 cut(s) 726, 892
BamHI GGATCC 1 cut(s) 369
BbrPI CACGTG 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 660
BccI CCATC 4 cut(s) 217, 290, 802, 1003
BceAI ACGGC 2 cut(s) 429, 777
BcoDI GTCTC 3 cut(s) 162, 853, 911
BfaI CTAG 4 cut(s) 45, 161, 416, 663
BfmI CTRYAG 1 cut(s) 213
BglII AGATCT 1 cut(s) 665
BmiI GGNNCC 3 cut(s) 51, 371, 791
BmsI GCATC 2 cut(s) 217, 832
BpiI GAAGAC 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 1 cut(s) 388
Bpu14I TTCGAA 2 cut(s) 726, 892
BpuEI CTTGAG 1 cut(s) 886
BsaAI YACGTR 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 318
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 638
Bse8I GATNNNNATC 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 318
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 1 cut(s) 638
BseRI GAGGAG 3 cut(s) 195, 932, 935
BsgI GTGCAG 1 cut(s) 848
Bsh1236I CGCG 2 cut(s) 499, 609
Bsh1285I CGRYCG 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 162, 853, 911
Bsp119I TTCGAA 2 cut(s) 726, 892
Bsp143I GATC 4 cut(s) 77, 369, 665, 971
BspACI CCGC 2 cut(s) 497, 719
BspCNI CTCAG 1 cut(s) 788
BspFNI CGCG 2 cut(s) 499, 609
BspLI GGNNCC 3 cut(s) 51, 371, 791
BspPI GGATC 2 cut(s) 364, 377
BspT104I TTCGAA 2 cut(s) 726, 892
BsrI ACTGG 1 cut(s) 638
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 4 cut(s) 77, 369, 665, 971
BssNAI GTATAC 1 cut(s) 990
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 990
BstACI GRCGYC 1 cut(s) 558
BstBAI YACGTR 1 cut(s) 334
BstBI TTCGAA 2 cut(s) 726, 892
BstDEI CTNAG 2 cut(s) 775, 868
BstFNI CGCG 2 cut(s) 499, 609
BstKTI GATC 4 cut(s) 80, 372, 668, 974
BstMAI GTCTC 3 cut(s) 162, 853, 911
BstMBI GATC 4 cut(s) 77, 369, 665, 971
BstMCI CGRYCG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 2 cut(s) 499, 609
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 369, 665
BstYI RGATCY 2 cut(s) 369, 665
BstZ17I GTATAC 1 cut(s) 990
BtgZI GCGATG 1 cut(s) 309
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
CseI GACGC 2 cut(s) 175, 488
CviAII CATG 3 cut(s) 169, 742, 984
CviJI RGCY 6 cut(s) 56, 237, 408, 450, 637, 792
CviKI_1 RGCY 6 cut(s) 56, 237, 408, 450, 637, 792
DdeI CTNAG 2 cut(s) 775, 868
DpnI GATC 4 cut(s) 79, 371, 667, 973
DpnII GATC 4 cut(s) 77, 369, 665, 971
DraI TTTAAA 3 cut(s) 223, 688, 703
DrdI GACNNNNNNGTC 2 cut(s) 575, 603
DseDI GACNNNNNNGTC 2 cut(s) 575, 603
Eco130I CCWWGG 1 cut(s) 318
Eco72I CACGTG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 3 cut(s) 172, 745, 987
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 3 cut(s) 168, 741, 983
FblI GTMKAC 3 cut(s) 88, 399, 989
FspBI CTAG 4 cut(s) 45, 161, 416, 663
GsuI CTGGAG 1 cut(s) 388
HgaI GACGC 2 cut(s) 175, 488
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 3 cut(s) 172, 745, 987
HinfI GANTC 9 cut(s) 10, 120, 155, 245, 343, 396, 437, 569, 894
HphI GGTGA 1 cut(s) 486
Hpy166II GTNNAC 3 cut(s) 89, 400, 990
Hpy188I TCNGA 3 cut(s) 262, 535, 1000
Hpy188III TCNNGA 2 cut(s) 566, 663
Hpy8I GTNNAC 3 cut(s) 89, 400, 990
HpyAV CCTTC 2 cut(s) 613, 725
HpyCH4IV ACGT 5 cut(s) 198, 333, 558, 598, 706
HpyCH4V TGCA 3 cut(s) 230, 312, 865
HpyF3I CTNAG 2 cut(s) 775, 868
HpySE526I ACGT 5 cut(s) 198, 333, 558, 598, 706
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 3 cut(s) 172, 745, 987
Kzo9I GATC 4 cut(s) 77, 369, 665, 971
LmnI GCTCC 1 cut(s) 789
LpnPI CCDG 3 cut(s) 66, 418, 619
LweI GCATC 2 cut(s) 217, 832
MaeI CTAG 4 cut(s) 45, 161, 416, 663
MaeII ACGT 5 cut(s) 198, 333, 558, 598, 706
MaeIII GTNAC 3 cut(s) 199, 476, 737
MalI GATC 4 cut(s) 79, 371, 667, 973
MboI GATC 4 cut(s) 77, 369, 665, 971
MboII GAAGA 7 cut(s) 5, 129, 278, 665, 776, 889, 937
MflI RGATCY 2 cut(s) 369, 665
MluCI AATT 5 cut(s) 256, 374, 658, 883, 953
MlyI GAGTC 3 cut(s) 164, 405, 563
MnlI CCTC 8 cut(s) 25, 216, 459, 500, 537, 910, 913, 996
MseI TTAA 7 cut(s) 222, 239, 687, 702, 882, 951, 959
MslI CAYNNNNRTG 2 cut(s) 294, 853
MvnI CGCG 2 cut(s) 499, 609
NdeII GATC 4 cut(s) 77, 369, 665, 971
NlaIII CATG 3 cut(s) 172, 745, 987
NlaIV GGNNCC 3 cut(s) 51, 371, 791
NmuCI GTSAC 1 cut(s) 737
NspV TTCGAA 2 cut(s) 726, 892
PcsI WCGNNNNNNNCGW 2 cut(s) 564, 1004
PfeI GAWTC 6 cut(s) 10, 120, 245, 343, 437, 894
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 3 cut(s) 163, 404, 563
PmaCI CACGTG 1 cut(s) 334
PmlI CACGTG 1 cut(s) 334
PpsI GAGTC 3 cut(s) 163, 404, 563
Ppu21I YACGTR 1 cut(s) 334
Psp1406I AACGTT 1 cut(s) 198
PspCI CACGTG 1 cut(s) 334
PspN4I GGNNCC 3 cut(s) 51, 371, 791
PsrI GAACNNNNNNTAC 2 cut(s) 973, 1005
PsuI RGATCY 2 cut(s) 369, 665
PvuI CGATCG 1 cut(s) 80
RseI CAYNNNNRTG 2 cut(s) 294, 853
SaqAI TTAA 7 cut(s) 222, 239, 687, 702, 882, 951, 959
Sau3AI GATC 4 cut(s) 77, 369, 665, 971
SchI GAGTC 3 cut(s) 164, 405, 563
SetI ASST 8 cut(s) 201, 336, 410, 452, 470, 561, 601, 709
SfaNI GCATC 2 cut(s) 217, 832
SfcI CTRYAG 1 cut(s) 213
SfuI TTCGAA 2 cut(s) 726, 892
SmiMI CAYNNNNRTG 2 cut(s) 294, 853
SmlI CTYRAG 1 cut(s) 901
SmoI CTYRAG 1 cut(s) 901
Sse9I AATT 5 cut(s) 256, 374, 658, 883, 953
SsiI CCGC 2 cut(s) 497, 719
SspI AATATT 1 cut(s) 628
SspMI CTAG 4 cut(s) 45, 161, 416, 663
StyI CCWWGG 1 cut(s) 318
TaiI ACGT 5 cut(s) 201, 336, 561, 601, 709
TaqI TCGA 5 cut(s) 8, 80, 726, 892, 915
TaqII GACCGA 1 cut(s) 129
TasI AATT 5 cut(s) 256, 374, 658, 883, 953
TfiI GAWTC 6 cut(s) 10, 120, 245, 343, 437, 894
Tru1I TTAA 7 cut(s) 222, 239, 687, 702, 882, 951, 959
Tru9I TTAA 7 cut(s) 222, 239, 687, 702, 882, 951, 959
TscAI CASTG 1 cut(s) 296
TseFI GTSAC 1 cut(s) 737
Tsp45I GTSAC 1 cut(s) 737
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 1 cut(s) 296
XbaI TCTAGA 1 cut(s) 662
XmiI GTMKAC 3 cut(s) 88, 399, 989
XspI CTAG 4 cut(s) 45, 161, 416, 663
ZraI GACGTC 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.