Belongs to the AAA ATPase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 719 |
| AciI | CCGC | 1 cut(s) | 719 |
| AclWI | GGATC | 3 cut(s) | 888, 901, 918 |
| AcsI | RAATTY | 2 cut(s) | 19, 275 |
| AcuI | CTGAAG | 2 cut(s) | 510, 627 |
| AfeI | AGCGCT | 1 cut(s) | 863 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 207, 232, 233 |
| AgsI | TTSAA | 3 cut(s) | 68, 271, 602 |
| AjiI | CACGTC | 1 cut(s) | 699 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 897, 929 |
| AluBI | AGCT | 5 cut(s) | 110, 123, 285, 646, 872 |
| AluI | AGCT | 5 cut(s) | 110, 123, 285, 646, 872 |
| Alw26I | GTCTC | 4 cut(s) | 349, 465, 554, 630 |
| AlwI | GGATC | 3 cut(s) | 888, 901, 918 |
| Aor51HI | AGCGCT | 1 cut(s) | 863 |
| AoxI | GGCC | 1 cut(s) | 772 |
| ApeKI | GCWGC | 2 cut(s) | 315, 643 |
| ApoI | RAATTY | 2 cut(s) | 19, 275 |
| AseI | ATTAAT | 2 cut(s) | 447, 677 |
| AspLEI | GCGC | 1 cut(s) | 864 |
| AspS9I | GGNCC | 2 cut(s) | 569, 773 |
| AsuC2I | CCSGG | 1 cut(s) | 244 |
| AsuHPI | GGTGA | 1 cut(s) | 184 |
| AvaII | GGWCC | 1 cut(s) | 569 |
| BamHI | GGATCC | 1 cut(s) | 893 |
| BanII | GRGCYC | 2 cut(s) | 258, 714 |
| BauI | CACGAG | 1 cut(s) | 212 |
| BbvI | GCAGC | 2 cut(s) | 327, 655 |
| BccI | CCATC | 2 cut(s) | 676, 884 |
| BceAI | ACGGC | 1 cut(s) | 649 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 629, 663 |
| BciVI | GTATCC | 3 cut(s) | 296, 634, 664 |
| BcnI | CCSGG | 1 cut(s) | 244 |
| BcoDI | GTCTC | 4 cut(s) | 349, 465, 554, 630 |
| BfaI | CTAG | 2 cut(s) | 207, 968 |
| BfmI | CTRYAG | 1 cut(s) | 850 |
| BfoI | RGCGCY | 1 cut(s) | 865 |
| BfuI | GTATCC | 3 cut(s) | 296, 634, 664 |
| BglII | AGATCT | 1 cut(s) | 687 |
| BisI | GCNGC | 2 cut(s) | 316, 644 |
| BlsI | GCNGC | 2 cut(s) | 317, 645 |
| Bme1390I | CCNGG | 1 cut(s) | 244 |
| Bme18I | GGWCC | 1 cut(s) | 569 |
| BmgBI | CACGTC | 1 cut(s) | 699 |
| BmgT120I | GGNCC | 2 cut(s) | 569, 773 |
| BmiI | GGNNCC | 2 cut(s) | 224, 895 |
| BmrFI | CCNGG | 1 cut(s) | 244 |
| BmsI | GCATC | 3 cut(s) | 192, 198, 601 |
| BpmI | CTGGAG | 1 cut(s) | 408 |
| Bpu10I | CCTNAGC | 1 cut(s) | 124 |
| BpuEI | CTTGAG | 3 cut(s) | 135, 355, 449 |
| BpuMI | CCSGG | 1 cut(s) | 244 |
| BsaBI | GATNNNNATC | 1 cut(s) | 686 |
| BsaI | GGTCTC | 1 cut(s) | 349 |
| BsaJI | CCNNGG | 1 cut(s) | 665 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 207, 232, 233 |
| Bse1I | ACTGG | 1 cut(s) | 434 |
| Bse8I | GATNNNNATC | 1 cut(s) | 686 |
| BseDI | CCNNGG | 1 cut(s) | 665 |
| BseGI | GGATG | 1 cut(s) | 895 |
| BseJI | GATNNNNATC | 1 cut(s) | 686 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 207, 232, 233 |
| BseMII | CTCAG | 1 cut(s) | 791 |
| BseNI | ACTGG | 1 cut(s) | 434 |
| BseRI | GAGGAG | 1 cut(s) | 830 |
| BseXI | GCAGC | 2 cut(s) | 327, 655 |
| Bsh1236I | CGCG | 1 cut(s) | 719 |
| BshFI | GGCC | 1 cut(s) | 774 |
| BsiSI | CCGG | 1 cut(s) | 244 |
| BslFI | GGGAC | 1 cut(s) | 454 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 207, 232, 233 |
| BsmAI | GTCTC | 4 cut(s) | 349, 465, 554, 630 |
| BsmBI | CGTCTC | 1 cut(s) | 630 |
| BsmFI | GGGAC | 1 cut(s) | 454 |
| BsnI | GGCC | 1 cut(s) | 774 |
| Bso31I | GGTCTC | 1 cut(s) | 349 |
| Bsp1286I | GDGCHC | 2 cut(s) | 258, 714 |
| Bsp143I | GATC | 3 cut(s) | 687, 893, 910 |
| BspACI | CCGC | 1 cut(s) | 719 |
| BspANI | GGCC | 1 cut(s) | 774 |
| BspCNI | CTCAG | 1 cut(s) | 790 |
| BspFNI | CGCG | 1 cut(s) | 719 |
| BspHI | TCATGA | 2 cut(s) | 9, 367 |
| BspLI | GGNNCC | 2 cut(s) | 224, 895 |
| BspPI | GGATC | 3 cut(s) | 888, 901, 918 |
| BspTNI | GGTCTC | 1 cut(s) | 349 |
| BsrI | ACTGG | 1 cut(s) | 434 |
| BssECI | CCNNGG | 1 cut(s) | 665 |
| BssMI | GATC | 3 cut(s) | 687, 893, 910 |
| BssSI | CACGAG | 1 cut(s) | 212 |
| Bst2BI | CACGAG | 1 cut(s) | 212 |
| Bst4CI | ACNGT | 1 cut(s) | 170 |
| Bst6I | CTCTTC | 3 cut(s) | 486, 519, 588 |
| BstC8I | GCNNGC | 1 cut(s) | 648 |
| BstDEI | CTNAG | 3 cut(s) | 124, 702, 777 |
| BstDSI | CCRYGG | 1 cut(s) | 665 |
| BstF5I | GGATG | 1 cut(s) | 895 |
| BstFNI | CGCG | 1 cut(s) | 719 |
| BstH2I | RGCGCY | 1 cut(s) | 865 |
| BstHHI | GCGC | 1 cut(s) | 864 |
| BstKTI | GATC | 3 cut(s) | 690, 896, 913 |
| BstMAI | GTCTC | 4 cut(s) | 349, 465, 554, 630 |
| BstMBI | GATC | 3 cut(s) | 687, 893, 910 |
| BstSCI | CCNGG | 1 cut(s) | 242 |
| BstSFI | CTRYAG | 1 cut(s) | 850 |
| BstUI | CGCG | 1 cut(s) | 719 |
| BstV1I | GCAGC | 2 cut(s) | 327, 655 |
| BstX2I | RGATCY | 3 cut(s) | 687, 893, 910 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 948 |
| BstYI | RGATCY | 3 cut(s) | 687, 893, 910 |
| BsuI | GTATCC | 3 cut(s) | 296, 634, 664 |
| BsuRI | GGCC | 1 cut(s) | 774 |
| BtgI | CCRYGG | 1 cut(s) | 665 |
| BtrI | CACGTC | 1 cut(s) | 699 |
| BtsCI | GGATG | 1 cut(s) | 895 |
| BtsI | GCAGTG | 1 cut(s) | 160 |
| BtsIMutI | CAGTG | 2 cut(s) | 160, 756 |
| Cac8I | GCNNGC | 1 cut(s) | 648 |
| CciI | TCATGA | 2 cut(s) | 9, 367 |
| CfoI | GCGC | 1 cut(s) | 864 |
| Cfr13I | GGNCC | 2 cut(s) | 569, 773 |
| CviAII | CATG | 6 cut(s) | 10, 93, 368, 402, 416, 898 |
| DdeI | CTNAG | 3 cut(s) | 124, 702, 777 |
| DpnI | GATC | 3 cut(s) | 689, 895, 912 |
| DpnII | GATC | 3 cut(s) | 687, 893, 910 |
| Eam1104I | CTCTTC | 3 cut(s) | 486, 519, 588 |
| EarI | CTCTTC | 3 cut(s) | 486, 519, 588 |
| Eco24I | GRGCYC | 2 cut(s) | 258, 714 |
| Eco31I | GGTCTC | 1 cut(s) | 349 |
| Eco47I | GGWCC | 1 cut(s) | 569 |
| Eco47III | AGCGCT | 1 cut(s) | 863 |
| Eco57I | CTGAAG | 2 cut(s) | 510, 627 |
| EcoT22I | ATGCAT | 1 cut(s) | 185 |
| EcoT38I | GRGCYC | 2 cut(s) | 258, 714 |
| Esp3I | CGTCTC | 1 cut(s) | 630 |
| FaeI | CATG | 6 cut(s) | 13, 96, 371, 405, 419, 901 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 728, 760, 897, 929 |
| FaqI | GGGAC | 1 cut(s) | 454 |
| FatI | CATG | 6 cut(s) | 9, 92, 367, 401, 415, 897 |
| Fnu4HI | GCNGC | 2 cut(s) | 316, 644 |
| FokI | GGATG | 1 cut(s) | 902 |
| FriOI | GRGCYC | 2 cut(s) | 258, 714 |
| Fsp4HI | GCNGC | 2 cut(s) | 316, 644 |
| FspBI | CTAG | 2 cut(s) | 207, 968 |
| GlaI | GCGC | 1 cut(s) | 863 |
| GluI | GCNGC | 2 cut(s) | 316, 644 |
| GsuI | CTGGAG | 1 cut(s) | 408 |
| HaeII | RGCGCY | 1 cut(s) | 865 |
| HaeIII | GGCC | 1 cut(s) | 774 |
| HapII | CCGG | 1 cut(s) | 244 |
| HhaI | GCGC | 1 cut(s) | 864 |
| Hin1II | CATG | 6 cut(s) | 13, 96, 371, 405, 419, 901 |
| Hin6I | GCGC | 1 cut(s) | 862 |
| HinP1I | GCGC | 1 cut(s) | 862 |
| HinfI | GANTC | 4 cut(s) | 42, 68, 602, 920 |
| HpaII | CCGG | 1 cut(s) | 244 |
| HphI | GGTGA | 1 cut(s) | 184 |
| Hpy188I | TCNGA | 3 cut(s) | 302, 607, 925 |
| Hpy188III | TCNNGA | 5 cut(s) | 10, 50, 334, 368, 425 |
| Hpy99I | CGWCG | 3 cut(s) | 65, 134, 170 |
| HpyAV | CCTTC | 2 cut(s) | 487, 924 |
| HpyCH4III | ACNGT | 1 cut(s) | 170 |
| HpyCH4IV | ACGT | 3 cut(s) | 60, 103, 698 |
| HpyCH4V | TGCA | 2 cut(s) | 183, 973 |
| HpyF3I | CTNAG | 3 cut(s) | 124, 702, 777 |
| HpySE526I | ACGT | 3 cut(s) | 60, 103, 698 |
| Hsp92II | CATG | 6 cut(s) | 13, 96, 371, 405, 419, 901 |
| HspAI | GCGC | 1 cut(s) | 862 |
| Kzo9I | GATC | 3 cut(s) | 687, 893, 910 |
| LpnPI | CCDG | 5 cut(s) | 257, 415, 438, 466, 676 |
| Lsp1109I | GCAGC | 2 cut(s) | 327, 655 |
| LweI | GCATC | 3 cut(s) | 192, 198, 601 |
| MaeI | CTAG | 2 cut(s) | 207, 968 |
| MaeII | ACGT | 3 cut(s) | 60, 103, 698 |
| MaeIII | GTNAC | 3 cut(s) | 418, 694, 935 |
| MalI | GATC | 3 cut(s) | 689, 895, 912 |
| MboI | GATC | 3 cut(s) | 687, 893, 910 |
| MboII | GAAGA | 6 cut(s) | 503, 536, 575, 620, 796, 832 |
| MfeI | CAATTG | 1 cut(s) | 942 |
| MflI | RGATCY | 3 cut(s) | 687, 893, 910 |
| MhlI | GDGCHC | 2 cut(s) | 258, 714 |
| MluCI | AATT | 6 cut(s) | 19, 275, 397, 434, 942, 979 |
| MlyI | GAGTC | 1 cut(s) | 51 |
| MnlI | CCTC | 5 cut(s) | 330, 399, 591, 786, 808 |
| Mph1103I | ATGCAT | 1 cut(s) | 185 |
| MseI | TTAA | 4 cut(s) | 390, 447, 677, 978 |
| MslI | CAYNNNNRTG | 4 cut(s) | 14, 91, 366, 884 |
| MspI | CCGG | 1 cut(s) | 244 |
| MspR9I | CCNGG | 1 cut(s) | 244 |
| MunI | CAATTG | 1 cut(s) | 942 |
| MvnI | CGCG | 1 cut(s) | 719 |
| NciI | CCSGG | 1 cut(s) | 244 |
| NdeII | GATC | 3 cut(s) | 687, 893, 910 |
| NlaIII | CATG | 6 cut(s) | 13, 96, 371, 405, 419, 901 |
| NlaIV | GGNNCC | 2 cut(s) | 224, 895 |
| NmuCI | GTSAC | 2 cut(s) | 418, 694 |
| NsiI | ATGCAT | 1 cut(s) | 185 |
| PagI | TCATGA | 2 cut(s) | 9, 367 |
| PfeI | GAWTC | 3 cut(s) | 68, 602, 920 |
| PkrI | GCNGC | 2 cut(s) | 317, 645 |
| PleI | GAGTC | 1 cut(s) | 50 |
| PpsI | GAGTC | 1 cut(s) | 50 |
| PshBI | ATTAAT | 2 cut(s) | 447, 677 |
| PspN4I | GGNNCC | 2 cut(s) | 224, 895 |
| PspPI | GGNCC | 2 cut(s) | 569, 773 |
| PsuI | RGATCY | 3 cut(s) | 687, 893, 910 |
| RseI | CAYNNNNRTG | 4 cut(s) | 14, 91, 366, 884 |
| SaqAI | TTAA | 4 cut(s) | 390, 447, 677, 978 |
| SatI | GCNGC | 2 cut(s) | 316, 644 |
| Sau3AI | GATC | 3 cut(s) | 687, 893, 910 |
| Sau96I | GGNCC | 2 cut(s) | 569, 773 |
| SchI | GAGTC | 1 cut(s) | 51 |
| ScrFI | CCNGG | 1 cut(s) | 244 |
| SduI | GDGCHC | 2 cut(s) | 258, 714 |
| SfaNI | GCATC | 3 cut(s) | 192, 198, 601 |
| SfcI | CTRYAG | 1 cut(s) | 850 |
| SinI | GGWCC | 1 cut(s) | 569 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 14, 91, 366, 884 |
| SmlI | CTYRAG | 3 cut(s) | 150, 334, 464 |
| SmoI | CTYRAG | 3 cut(s) | 150, 334, 464 |
| Sse9I | AATT | 6 cut(s) | 19, 275, 397, 434, 942, 979 |
| SsiI | CCGC | 1 cut(s) | 719 |
| SspMI | CTAG | 2 cut(s) | 207, 968 |
| StyD4I | CCNGG | 1 cut(s) | 242 |
| TaaI | ACNGT | 1 cut(s) | 170 |
| TaiI | ACGT | 3 cut(s) | 63, 106, 701 |
| TaqI | TCGA | 2 cut(s) | 252, 279 |
| TasI | AATT | 6 cut(s) | 19, 275, 397, 434, 942, 979 |
| TfiI | GAWTC | 3 cut(s) | 68, 602, 920 |
| Tru1I | TTAA | 4 cut(s) | 390, 447, 677, 978 |
| Tru9I | TTAA | 4 cut(s) | 390, 447, 677, 978 |
| TscAI | CASTG | 2 cut(s) | 167, 756 |
| TseFI | GTSAC | 2 cut(s) | 418, 694 |
| TseI | GCWGC | 2 cut(s) | 315, 643 |
| Tsp45I | GTSAC | 2 cut(s) | 418, 694 |
| TspDTI | ATGAA | 7 cut(s) | 32, 356, 364, 390, 404, 796, 914 |
| TspGWI | ACGGA | 1 cut(s) | 576 |
| TspRI | CASTG | 2 cut(s) | 167, 756 |
| VpaK11BI | GGWCC | 1 cut(s) | 569 |
| VspI | ATTAAT | 2 cut(s) | 447, 677 |
| XapI | RAATTY | 2 cut(s) | 19, 275 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 904 |
| XspI | CTAG | 2 cut(s) | 207, 968 |
| Zsp2I | ATGCAT | 1 cut(s) | 185 |