AT3G04250

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
1121950 .. 1122828
879 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04250.1

Sequence Viewer

Length: 879 bp
ATGTACAGGTACGCTCTAGGGTACGTGAACAAGAACAACTCTTGTCGTAGCCACAAAGTCTTGAGGTTTATAGATCATTACATCTCTTCGGCCGTGAACCACGCACCCGAAAACCGGTTTTCATGGTATGAAATCTACGATATTGAAACCGATTTATGGACAACTCTTGATGTCCCTCCATATTGGATAGTATCGTGTTATCAGCGTGGTGTTTCTCTCAAGGGAAATACCTACTTTTGTGCTACAAAAAGTAACGCAATAAACAAAATCGACGATCACATAATCTGTTTTGATTTCACAAGTGAGAGATTTGGGCCACTTTTGCGTCTACCGTTTAGCGCTGGCCATGATGACTATGTGACTTTATCTTGTGTTAGAGAAGAGAAGCTTGCGGCTTTACTTACGCACAACGACTGGAATCCATATGAGTTTGAGATATGGATCACGACTAAGATCGAGTATGATAAAGTGTCGTGGAGCAAGATCTTGGAAATGGATACAGGACCATTGGCTGACATGCCGATTGCATATATACATGGGAGTTTCTTCATTGACGAGGAGAAGAAAGTCTCCATGGTTTTTGATCACCACAGAGTCAACATCATTGGAAAAACTGGATATTTTAGAGAATTGGATCTCCGAGTACATGTAGCCGGCATAAAGTGTAGGCCACATTTGTGCCCTTACGTTCCAAATTCAATGCAAATCAAACAACCTCCGGGAGGCAAAAGGAAACGAAAACCCATTCTAGAACAGCGTCGATATGACCAAAACATGTTGAGAGTTGCCGCCTTTATAAAGCGAACCAAGCAGGAGGATAATAAATGGAAAAAACGAGTCCTCGAGGGTCTGAAGAAGCAAACGAATTTTAAAGCCTAA

Protein Analysis

292

Amino Acids

34.38

Weight (kDa)

9.14

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 2 - 236 2.8e-71 F-box associated beta propeller domain
FBA_3 PF08268 17 - 162 2.2e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
AbsI CCTCGAGG 1 cut(s) 842
AccI GTMKAC 1 cut(s) 328
AciI CCGC 2 cut(s) 392, 789
AclWI GGATC 2 cut(s) 449, 642
AcoI YGGCCR 2 cut(s) 90, 343
AcsI RAATTY 2 cut(s) 694, 865
AcuI CTGAAG 1 cut(s) 872
AfaI GTAC 4 cut(s) 5, 11, 23, 645
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 3 cut(s) 114, 156, 722
AflIII ACRYGT 2 cut(s) 646, 774
AgeI ACCGGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 146, 699
AleI CACNNNNGTG 1 cut(s) 676
AluBI AGCT 1 cut(s) 388
AluI AGCT 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 574
AlwI GGATC 2 cut(s) 449, 642
Ama87I CYCGRG 1 cut(s) 842
Aor51HI AGCGCT 1 cut(s) 340
AoxI GGCC 4 cut(s) 90, 314, 343, 668
ApoI RAATTY 2 cut(s) 694, 865
AsiGI ACCGGT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 314, 503
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 578
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 345
BceAI ACGGC 1 cut(s) 77
BciVI GTATCC 1 cut(s) 490
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 1 cut(s) 574
BfaI CTAG 2 cut(s) 17, 749
BfoI RGCGCY 1 cut(s) 342
BfuI GTATCC 1 cut(s) 490
BglII AGATCT 1 cut(s) 483
BisI GCNGC 2 cut(s) 393, 789
BlsI GCNGC 2 cut(s) 394, 790
Bme1390I CCNGG 1 cut(s) 720
Bme18I GGWCC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 2 cut(s) 314, 503
BmrFI CCNGG 1 cut(s) 720
BpuEI CTTGAG 2 cut(s) 82, 203
BpuMI CCSGG 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 25
BsaBI GATNNNNATC 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 573
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 156, 722
Bse118I RCCGGY 2 cut(s) 114, 653
Bse1I ACTGG 2 cut(s) 419, 619
Bse8I GATNNNNATC 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 573
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 3 cut(s) 114, 156, 722
BseNI ACTGG 2 cut(s) 419, 619
BseRI GAGGAG 1 cut(s) 572
BseSI GKGCMC 1 cut(s) 683
BseX3I CGGCCG 1 cut(s) 90
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 4 cut(s) 92, 316, 345, 670
BshTI ACCGGT 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 3 cut(s) 115, 654, 719
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 3 cut(s) 114, 156, 722
BsmAI GTCTC 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 4 cut(s) 92, 316, 345, 670
BsoBI CYCGRG 1 cut(s) 842
Bsp1286I GDGCHC 1 cut(s) 683
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 2 cut(s) 392, 789
BspANI GGCC 4 cut(s) 92, 316, 345, 670
BspPI GGATC 2 cut(s) 449, 642
BsrFI RCCGGY 2 cut(s) 114, 653
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 419, 619
BssAI RCCGGY 2 cut(s) 114, 653
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
BssT1I CCWWGG 1 cut(s) 573
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 2 cut(s) 91, 375
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 343, 390, 655
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 573
BstENI CCTNNNNNAGG 1 cut(s) 720
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 7 cut(s) 76, 277, 444, 456, 486, 586, 637
BstMAI GTCTC 1 cut(s) 574
BstMBI GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 322, 808
BstNSI RCATGY 3 cut(s) 520, 650, 778
BstSCI CCNGG 1 cut(s) 718
BstSLI GKGCMC 1 cut(s) 683
BstX2I RGATCY 2 cut(s) 483, 634
BstYI RGATCY 2 cut(s) 483, 634
BstZI CGGCCG 1 cut(s) 90
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 4 cut(s) 92, 316, 345, 670
BtgI CCRYGG 1 cut(s) 573
Cac8I GCNNGC 3 cut(s) 343, 390, 655
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 2 cut(s) 114, 653
Cfr13I GGNCC 2 cut(s) 314, 503
CseI GACGC 2 cut(s) 314, 746
Csp6I GTAC 4 cut(s) 4, 10, 22, 644
CspAI ACCGGT 1 cut(s) 114
CviAII CATG 7 cut(s) 123, 347, 517, 536, 574, 647, 775
CviQI GTAC 4 cut(s) 4, 10, 22, 644
DdeI CTNAG 1 cut(s) 450
DpnI GATC 7 cut(s) 75, 276, 443, 455, 485, 585, 636
DpnII GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 2 cut(s) 90, 343
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 91, 375
EarI CTCTTC 2 cut(s) 91, 375
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 503
Eco47III AGCGCT 1 cut(s) 340
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 872
Eco88I CYCGRG 1 cut(s) 842
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 7 cut(s) 126, 350, 520, 539, 577, 650, 778
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 158
FatI CATG 7 cut(s) 122, 346, 516, 535, 573, 646, 774
FauNDI CATATG 1 cut(s) 424
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 2 cut(s) 393, 789
Fsp4HI GCNGC 2 cut(s) 393, 789
FspBI CTAG 2 cut(s) 17, 749
GlaI GCGC 1 cut(s) 340
GluI GCNGC 2 cut(s) 393, 789
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 4 cut(s) 92, 316, 345, 670
HapII CCGG 3 cut(s) 115, 654, 719
HgaI GACGC 2 cut(s) 314, 746
HhaI GCGC 1 cut(s) 341
Hin1II CATG 7 cut(s) 126, 350, 520, 539, 577, 650, 778
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 3 cut(s) 418, 594, 837
HpaII CCGG 3 cut(s) 115, 654, 719
HphI GGTGA 1 cut(s) 578
Hpy166II GTNNAC 4 cut(s) 28, 97, 329, 598
Hpy188I TCNGA 2 cut(s) 641, 852
Hpy188III TCNNGA 4 cut(s) 61, 167, 445, 749
Hpy8I GTNNAC 4 cut(s) 28, 97, 329, 598
Hpy99I CGWCG 2 cut(s) 275, 762
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 2 cut(s) 24, 687
HpyCH4V TGCA 2 cut(s) 527, 703
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 808
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 24, 687
Hsp92II CATG 7 cut(s) 126, 350, 520, 539, 577, 650, 778
HspAI GCGC 1 cut(s) 339
KroI GCCGGC 1 cut(s) 653
KroNI GCCGGC 1 cut(s) 655
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
LmnI GCTCC 1 cut(s) 477
LpnPI CCDG 8 cut(s) 128, 327, 400, 486, 600, 667, 732, 797
MaeI CTAG 2 cut(s) 17, 749
MaeII ACGT 2 cut(s) 24, 687
MaeIII GTNAC 2 cut(s) 251, 358
MalI GATC 7 cut(s) 75, 276, 443, 455, 485, 585, 636
MboI GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
MboII GAAGA 5 cut(s) 78, 392, 538, 574, 865
MflI RGATCY 2 cut(s) 483, 634
MhlI GDGCHC 1 cut(s) 683
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 3 cut(s) 629, 694, 865
MluNI TGGCCA 1 cut(s) 345
MlyI GAGTC 2 cut(s) 603, 846
MnlI CCTC 8 cut(s) 57, 186, 550, 716, 726, 808, 838, 851
Mox20I TGGCCA 1 cut(s) 345
MroNI GCCGGC 1 cut(s) 653
MscI TGGCCA 1 cut(s) 345
MseI TTAA 1 cut(s) 870
MslI CAYNNNNRTG 1 cut(s) 676
Msp20I TGGCCA 1 cut(s) 345
MspI CCGG 3 cut(s) 115, 654, 719
MspR9I CCNGG 1 cut(s) 720
MwoI GCNNNNNNNGC 2 cut(s) 322, 808
NaeI GCCGGC 1 cut(s) 655
NciI CCSGG 1 cut(s) 720
NcoI CCATGG 1 cut(s) 573
NdeI CATATG 1 cut(s) 424
NdeII GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
NgoMIV GCCGGC 1 cut(s) 653
NlaIII CATG 7 cut(s) 126, 350, 520, 539, 577, 650, 778
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 3 cut(s) 520, 650, 778
OliI CACNNNNGTG 1 cut(s) 676
PaeR7I CTCGAG 1 cut(s) 842
PciI ACATGT 2 cut(s) 646, 774
PdiI GCCGGC 1 cut(s) 655
PfeI GAWTC 1 cut(s) 418
PfoI TCCNGGA 1 cut(s) 718
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 2 cut(s) 394, 790
PleI GAGTC 2 cut(s) 602, 845
PpsI GAGTC 2 cut(s) 602, 845
Ppu21I YACGTR 1 cut(s) 25
PscI ACATGT 2 cut(s) 646, 774
PsiI TTATAA 1 cut(s) 797
PspPI GGNCC 2 cut(s) 314, 503
PspXI VCTCGAGB 1 cut(s) 842
PsuI RGATCY 2 cut(s) 483, 634
RsaI GTAC 4 cut(s) 5, 11, 23, 645
RsaNI GTAC 4 cut(s) 4, 10, 22, 644
RseI CAYNNNNRTG 1 cut(s) 676
SaqAI TTAA 1 cut(s) 870
SatI GCNGC 2 cut(s) 393, 789
Sau3AI GATC 7 cut(s) 73, 274, 441, 453, 483, 583, 634
Sau96I GGNCC 2 cut(s) 314, 503
SchI GAGTC 2 cut(s) 603, 846
ScrFI CCNGG 1 cut(s) 720
SduI GDGCHC 1 cut(s) 683
SetI ASST 7 cut(s) 11, 27, 68, 233, 390, 690, 718
Sfr274I CTCGAG 1 cut(s) 842
SinI GGWCC 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 842
SmiMI CAYNNNNRTG 1 cut(s) 676
SmlI CTYRAG 3 cut(s) 61, 218, 842
SmoI CTYRAG 3 cut(s) 61, 218, 842
Sse9I AATT 3 cut(s) 629, 694, 865
SsiI CCGC 2 cut(s) 392, 789
SspMI CTAG 2 cut(s) 17, 749
StyD4I CCNGG 1 cut(s) 718
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 2 cut(s) 27, 690
TaqI TCGA 4 cut(s) 270, 456, 760, 843
TasI AATT 3 cut(s) 629, 694, 865
TatI WGTACW 2 cut(s) 3, 643
TauI GCSGC 2 cut(s) 395, 791
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 3 cut(s) 111, 144, 538
VpaK11BI GGWCC 1 cut(s) 503
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 2 cut(s) 694, 865
XbaI TCTAGA 1 cut(s) 748
XceI RCATGY 3 cut(s) 520, 650, 778
XhoI CTCGAG 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 2 cut(s) 17, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.