AT3G22730

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Reverse (-)
8031341 .. 8032459
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22730.1

Sequence Viewer

Length: 1119 bp
ATGATGATGTCCGATCTTTCTTTGGATTTGGTAGAGGAAATACTCTCTAGGGTTCCAGCCACATCTCTCAAACGATTACGATCTACTTGCAAACTATGGAACGCTTTATTTAAAAACCCGGGATTTACCAAGAAGCAATTTCTTAAAGCACCAAAGGAGTCACTGGTTCTCATGTTGAAGGAGTATAGCGTTTGTCCAATGATCGCCAATCTCAGCGTTTCTGCTCCGTCTATAGAGTTTAAAGGTGCACTTAACCTAAAGAATTATCCTCCTTATTCAGAAGAAGTCGATATACATGAAGCTTGTCACTGTGATGGTTTATTGCTATGTACAACTATGGACTATAGAATAGTGGTTTGGAACCCGTGTTTGGGGGAAACAAGGTGGATCCGATGGCCCAAAAACATTTACTCTAGGTTTGCTCTAGGATACGAGAAAAACAAATATGGCCGTATCTACAAAATCTTGAGGTGTTGGGATCGTCACAATTCACCAACTGGCCGAGTTGATGAGTTTGAAATCTATGAGTTTAGCTCTGATTCATGGAGGGTTCTTGATCTCGTCGCTCTTGATTGCCACATAGCATCACATATTGGCGTTTCTTTCAAGGGAAATACTTATTGGCTTGCGTCTGATAAAAAAGACAAATATGGACTCTTGCTTTGCTTCGATTTTACAACAGAAAGATTTACGCGTCTGTGTCTTCCACCATCTCAGGATGTGTCTAAGATGGCTCTATCAGTTGTTGGAGGAAAACAACTCTCACTGTTAAGTCAAAGTGATAGTACATCAAAGATAATGGAGATGTGGGTGACCAATATTATTGAAGATGTACTGATGTGGAGCAAATCTTTTACCGTGGATATACCCATTCGCGGTGATTATTGTCCGTATTTGACGAGTTATCTAGTCGACGAAGAAAAGAAAGTGGCTGTGTGTTATAATGAAAATTTTGAGAAAAGCAACAAGAAAGTATACATTATTGGAGAGGACAATGGATACTACACAGAAATCCCTTTAGTAGAATCTAGTTACCAAGTGTGGGGATATCCAATTATTTTCAATTATGTTCCAAGTTTGGTTCAAATCGAGGAAGGTGGATGCATTATAAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

372

Amino Acids

42.89

Weight (kDa)

6.06

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 44 3e-09 F-box domain
FBA_1 PF07734 56 - 363 3.4e-99 F-box associated beta propeller domain
FBA_3 PF08268 99 - 286 1.6e-15 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 942, 1109
AccI GTMKAC 2 cut(s) 912, 975
AccII CGCG 2 cut(s) 694, 876
AciI CCGC 1 cut(s) 876
AclWI GGATC 3 cut(s) 382, 395, 486
AcoI YGGCCR 2 cut(s) 448, 499
AcsI RAATTY 1 cut(s) 949
AfaI GTAC 3 cut(s) 331, 787, 834
AfiI CCNNNNNNNGG 4 cut(s) 370, 371, 875, 1042
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 6 cut(s) 178, 518, 607, 827, 1063, 1085
AjuI GAANNNNNNNTTGG 6 cut(s) 340, 353, 372, 385, 604, 636
AluBI AGCT 2 cut(s) 302, 534
AluI AGCT 2 cut(s) 302, 534
Alw21I GWGCWC 1 cut(s) 250
Alw44I GTGCAC 1 cut(s) 246
AlwI GGATC 3 cut(s) 382, 395, 486
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 3 cut(s) 395, 448, 499
ApaLI GTGCAC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 949
ArsI GACNNNNNNTTYG 2 cut(s) 645, 677
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 2 cut(s) 119, 120
AsuHPI GGTGA 3 cut(s) 483, 823, 890
AvaI CYCGRG 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 250
BamHI GGATCC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 1 cut(s) 250
BccI CCATC 4 cut(s) 308, 387, 718, 724
BceAI ACGGC 1 cut(s) 435
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciVI GTATCC 2 cut(s) 422, 992
BcnI CCSGG 2 cut(s) 119, 120
BfaI CTAG 5 cut(s) 48, 414, 425, 908, 1029
BfmI CTRYAG 2 cut(s) 231, 343
BfuI GTATCC 2 cut(s) 422, 992
Bme1390I CCNGG 2 cut(s) 119, 120
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 3 cut(s) 54, 362, 389
BmrFI CCNGG 2 cut(s) 119, 120
BmsI GCATC 2 cut(s) 593, 1091
BpiI GAAGAC 1 cut(s) 695
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpuEI CTTGAG 1 cut(s) 487
BpuMI CCSGG 2 cut(s) 119, 120
BsaBI GATNNNNATC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 118, 858
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 371, 875, 1042
Bse1I ACTGG 2 cut(s) 168, 502
Bse8I GATNNNNATC 1 cut(s) 79
BseDI CCNNGG 2 cut(s) 118, 858
BseGI GGATG 2 cut(s) 724, 1106
BseJI GATNNNNATC 1 cut(s) 79
BseLI CCNNNNNNNGG 4 cut(s) 370, 371, 875, 1042
BseMII CTCAG 2 cut(s) 226, 728
BseNI ACTGG 2 cut(s) 168, 502
BseSI GKGCMC 1 cut(s) 250
Bsh1236I CGCG 2 cut(s) 694, 876
BshFI GGCC 3 cut(s) 397, 450, 501
BsiHKAI GWGCWC 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 4 cut(s) 370, 371, 875, 1042
BsnI GGCC 3 cut(s) 397, 450, 501
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 250
Bsp1407I TGTACA 1 cut(s) 329
Bsp143I GATC 6 cut(s) 13, 80, 201, 387, 478, 556
BspACI CCGC 1 cut(s) 876
BspANI GGCC 3 cut(s) 397, 450, 501
BspCNI CTCAG 2 cut(s) 225, 727
BspFNI CGCG 2 cut(s) 694, 876
BspLI GGNNCC 3 cut(s) 54, 362, 389
BspPI GGATC 3 cut(s) 382, 395, 486
BsrGI TGTACA 1 cut(s) 329
BsrI ACTGG 2 cut(s) 168, 502
BssECI CCNNGG 2 cut(s) 118, 858
BssMI GATC 6 cut(s) 13, 80, 201, 387, 478, 556
BssNAI GTATAC 1 cut(s) 976
Bst1107I GTATAC 1 cut(s) 976
Bst4CI ACNGT 3 cut(s) 311, 768, 859
BstAUI TGTACA 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 3 cut(s) 212, 714, 726
BstDSI CCRYGG 1 cut(s) 858
BstEII GGTNACC 1 cut(s) 811
BstF5I GGATG 2 cut(s) 724, 1106
BstFNI CGCG 2 cut(s) 694, 876
BstKTI GATC 6 cut(s) 16, 83, 204, 390, 481, 559
BstMBI GATC 6 cut(s) 13, 80, 201, 387, 478, 556
BstPI GGTNACC 1 cut(s) 811
BstSCI CCNGG 2 cut(s) 117, 118
BstSFI CTRYAG 2 cut(s) 231, 343
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 2 cut(s) 694, 876
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BstZ17I GTATAC 1 cut(s) 976
BsuI GTATCC 2 cut(s) 422, 992
BsuRI GGCC 3 cut(s) 397, 450, 501
BtgI CCRYGG 1 cut(s) 858
BtsCI GGATG 2 cut(s) 724, 1106
BtsIMutI CAGTG 3 cut(s) 161, 307, 764
Cac8I GCNNGC 1 cut(s) 627
Cfr13I GGNCC 1 cut(s) 396
Cfr9I CCCGGG 1 cut(s) 118
CseI GACGC 2 cut(s) 618, 683
Csp6I GTAC 3 cut(s) 330, 786, 833
CviAII CATG 3 cut(s) 172, 296, 543
CviJI RGCY 9 cut(s) 59, 302, 397, 450, 501, 534, 625, 734, 932
CviKI_1 RGCY 9 cut(s) 59, 302, 397, 450, 501, 534, 625, 734, 932
CviQI GTAC 3 cut(s) 330, 786, 833
DdeI CTNAG 3 cut(s) 212, 714, 726
DpnI GATC 6 cut(s) 15, 82, 203, 389, 480, 558
DpnII GATC 6 cut(s) 13, 80, 201, 387, 478, 556
DraI TTTAAA 2 cut(s) 112, 241
EaeI YGGCCR 2 cut(s) 448, 499
Eco32I GATATC 1 cut(s) 1049
Eco88I CYCGRG 1 cut(s) 118
Eco91I GGTNACC 1 cut(s) 811
EcoO65I GGTNACC 1 cut(s) 811
EcoRV GATATC 1 cut(s) 1049
EcoT22I ATGCAT 1 cut(s) 1106
FaeI CATG 3 cut(s) 175, 299, 546
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 3 cut(s) 171, 295, 542
FblI GTMKAC 2 cut(s) 912, 975
FokI GGATG 2 cut(s) 731, 1113
FspBI CTAG 5 cut(s) 48, 414, 425, 908, 1029
HaeIII GGCC 3 cut(s) 397, 450, 501
HapII CCGG 1 cut(s) 119
HgaI GACGC 2 cut(s) 618, 683
Hin1II CATG 3 cut(s) 175, 299, 546
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 4 cut(s) 158, 539, 654, 1025
HpaII CCGG 1 cut(s) 119
HphI GGTGA 3 cut(s) 483, 823, 890
Hpy166II GTNNAC 3 cut(s) 248, 913, 976
Hpy188I TCNGA 5 cut(s) 13, 280, 392, 538, 634
Hpy188III TCNNGA 4 cut(s) 466, 554, 569, 716
Hpy8I GTNNAC 3 cut(s) 248, 913, 976
Hpy99I CGWCG 2 cut(s) 566, 917
HpyAV CCTTC 2 cut(s) 172, 1088
HpyCH4III ACNGT 3 cut(s) 311, 768, 859
HpyCH4V TGCA 3 cut(s) 90, 248, 1104
HpyF3I CTNAG 3 cut(s) 212, 714, 726
Hsp92II CATG 3 cut(s) 175, 299, 546
Kzo9I GATC 6 cut(s) 13, 80, 201, 387, 478, 556
LmnI GCTCC 2 cut(s) 229, 843
LpnPI CCDG 5 cut(s) 69, 132, 149, 483, 701
LweI GCATC 2 cut(s) 593, 1091
MaeI CTAG 5 cut(s) 48, 414, 425, 908, 1029
MaeIII GTNAC 5 cut(s) 159, 305, 482, 811, 1031
MalI GATC 6 cut(s) 15, 82, 203, 389, 480, 558
MboI GATC 6 cut(s) 13, 80, 201, 387, 478, 556
MboII GAAGA 4 cut(s) 293, 695, 839, 929
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 6 cut(s) 137, 262, 487, 949, 1053, 1063
MluI ACGCGT 1 cut(s) 692
MlyI GAGTC 2 cut(s) 167, 648
MmeI TCCRAC 1 cut(s) 727
MnlI CCTC 7 cut(s) 28, 279, 462, 540, 743, 982, 1084
Mph1103I ATGCAT 1 cut(s) 1106
MseI TTAA 5 cut(s) 111, 144, 240, 252, 770
MslI CAYNNNNRTG 1 cut(s) 312
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 2 cut(s) 119, 120
MvnI CGCG 2 cut(s) 694, 876
NciI CCSGG 2 cut(s) 119, 120
NdeII GATC 6 cut(s) 13, 80, 201, 387, 478, 556
NlaIII CATG 3 cut(s) 175, 299, 546
NlaIV GGNNCC 3 cut(s) 54, 362, 389
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 4 cut(s) 159, 305, 482, 811
NsiI ATGCAT 1 cut(s) 1106
PfeI GAWTC 2 cut(s) 539, 1025
PleI GAGTC 2 cut(s) 166, 648
PpsI GAGTC 2 cut(s) 166, 648
PsiI TTATAA 2 cut(s) 942, 1109
PspEI GGTNACC 1 cut(s) 811
PspN4I GGNNCC 3 cut(s) 54, 362, 389
PspPI GGNCC 1 cut(s) 396
PsuI RGATCY 1 cut(s) 387
RsaI GTAC 3 cut(s) 331, 787, 834
RsaNI GTAC 3 cut(s) 330, 786, 833
RseI CAYNNNNRTG 1 cut(s) 312
SalI GTCGAC 1 cut(s) 911
SaqAI TTAA 5 cut(s) 111, 144, 240, 252, 770
Sau3AI GATC 6 cut(s) 13, 80, 201, 387, 478, 556
Sau96I GGNCC 1 cut(s) 396
SchI GAGTC 2 cut(s) 167, 648
ScrFI CCNGG 2 cut(s) 119, 120
SduI GDGCHC 1 cut(s) 250
SetI ASST 8 cut(s) 247, 258, 304, 386, 419, 473, 536, 1099
SfaNI GCATC 2 cut(s) 593, 1091
SfcI CTRYAG 2 cut(s) 231, 343
SmaI CCCGGG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 6 cut(s) 137, 262, 487, 949, 1053, 1063
SsiI CCGC 1 cut(s) 876
SspI AATATT 1 cut(s) 820
SspMI CTAG 5 cut(s) 48, 414, 425, 908, 1029
StyD4I CCNGG 2 cut(s) 117, 118
TaaI ACNGT 3 cut(s) 311, 768, 859
TaqI TCGA 4 cut(s) 288, 669, 912, 1089
TasI AATT 6 cut(s) 137, 262, 487, 949, 1053, 1063
TatI WGTACW 3 cut(s) 329, 785, 832
TfiI GAWTC 2 cut(s) 539, 1025
Tru1I TTAA 5 cut(s) 111, 144, 240, 252, 770
Tru9I TTAA 5 cut(s) 111, 144, 240, 252, 770
TscAI CASTG 3 cut(s) 168, 314, 771
TseFI GTSAC 4 cut(s) 159, 305, 482, 811
Tsp45I GTSAC 4 cut(s) 159, 305, 482, 811
TspDTI ATGAA 3 cut(s) 312, 531, 960
TspGWI ACGGA 2 cut(s) 216, 879
TspMI CCCGGG 1 cut(s) 118
TspRI CASTG 3 cut(s) 168, 314, 771
VneI GTGCAC 1 cut(s) 246
XapI RAATTY 1 cut(s) 949
XmaI CCCGGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 912, 975
XspI CTAG 5 cut(s) 48, 414, 425, 908, 1029
Zsp2I ATGCAT 1 cut(s) 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.