AT2G27520

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000186
Physical Location & Seq
Forward (+)
11762061 .. 11763143
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27520.1

Sequence Viewer

Length: 1044 bp
ATGGTGAGATTGGATCTTCCATGGGATTTGGTAGATGAAATACTCTCTCGACTTCCCGCAACATCTCTAGGTCGACTACGATTTACATGCAAACGATGGAATGCTTTATTCAAAGATCCGGAATTCATCACAAAGCAATTTCATAAAGCAGCAAAGCAAGATTTGGTTCTCATGTTGAGTAATTTTGGGGTTTACTCGATGAGTACCAATCTCAAAGAAATTCCCAACAACATTGAAATAGCGCAAGTTTTTCATTGCAACGGCTTATTGTTATGTTCCACGGAAGAGGGTAACAAGACTAAACTCGTGGTTGTGAATCCGTGTACTGGTCAGACCAGGTGGATCGAACCAAGAACTGATTACAATTATAATCATGATATCGCTCTAGGTTACGGAAACAACAGCACCAAGAAATCGTACGACAGCTACAAAATCTTGAGGATTACGTATGGTTGCAAACTCGTTGAAATCTTTGAGCTAAAGTCTAATTCATGGAGGGTTCTTTCTAAAGTCCATCCCAACGTGGAAAAACACTATTATGGAGGCGTGTCTTTCAAGGGAAATACCTACTGGCTTTCGTATACGAAATTCAATATATTGAGTTTTGATTTCACAACAGAGACATTTAGAAGTGTGCCTCTTCCGTTTCTGTATCAGGATGGTTTCGTTACTTTAGCTCTATCAGTTGTTAGAGAAGAGCAACTTCTGTTGTTACGTTCGAGGTTTGATATGGGACAGGTTGGGATATGGATGTGTAATAAGATTGATACTGAAACTGTGTTGTCGTGGAGCAAATCCTTTACATTGGAATTTGATTCTCTGCGTGATCTTCCTGTTATGAGTATTCTGAGAATCTTCATTGAAGAAGACAAGAAAGTGATTGTTGTGGATTGTGATGACAGATGGAAGGAGAACATGATATACATTGTTGGAAAGAATGGATTCAAAAAGTTAAGTTATGAGAAAGACCGATCTAATCTTTGGCGTCTGCCGTTTTTCTTTAGTTATGTTCCGAGTTTAGTTGGTTTATATCCACCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

347

Amino Acids

40.53

Weight (kDa)

8.78

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 43 1.7e-11 F-box domain
F-box-like PF12937 5 - 42 1.3e-07 F-box-like
FBA_1 PF07734 56 - 341 6.9e-83 F-box associated beta propeller domain
FBA_3 PF08268 69 - 216 6.3e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AccI GTMKAC 2 cut(s) 73, 581
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 1 cut(s) 57
AclWI GGATC 3 cut(s) 21, 110, 350
AcsI RAATTY 4 cut(s) 122, 219, 587, 809
AcyI GRCGYC 1 cut(s) 985
AfaI GTAC 3 cut(s) 205, 325, 419
AfiI CCNNNNNNNGG 1 cut(s) 326
AgsI TTSAA 7 cut(s) 112, 236, 467, 556, 592, 863, 946
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 3 cut(s) 426, 478, 677
AluI AGCT 3 cut(s) 426, 478, 677
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 3 cut(s) 21, 110, 350
Aor13HI TCCGGA 1 cut(s) 118
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 4 cut(s) 122, 219, 587, 809
Asp700I GAANNNNTTC 1 cut(s) 941
AspLEI GCGC 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 16
BauI CACGAG 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 873
BbvI GCAGC 1 cut(s) 161
BccI CCATC 4 cut(s) 90, 522, 653, 897
BceAI ACGGC 2 cut(s) 277, 976
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 2 cut(s) 68, 386
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 985
BsaJI CCNNGG 2 cut(s) 20, 279
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 331, 575
Bse3DI GCAATG 1 cut(s) 253
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 20, 279
BseGI GGATG 3 cut(s) 514, 664, 756
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 2 cut(s) 331, 575
BseXI GCAGC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 119
BsiWI CGTACG 1 cut(s) 417
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 747
BsmI GAATGC 1 cut(s) 106
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 5 cut(s) 13, 115, 342, 826, 971
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 840
BspEI TCCGGA 1 cut(s) 118
BspHI TCATGA 1 cut(s) 373
BspPI GGATC 3 cut(s) 21, 110, 350
BspQI GCTCTTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 253
BsrI ACTGG 2 cut(s) 331, 575
BssECI CCNNGG 2 cut(s) 20, 279
BssMI GATC 5 cut(s) 13, 115, 342, 826, 971
BssNAI GTATAC 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 985
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 20
Bst1107I GTATAC 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 778
Bst6I CTCTTC 3 cut(s) 279, 645, 690
BstACI GRCGYC 1 cut(s) 985
BstBAI YACGTR 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 848
BstDSI CCRYGG 2 cut(s) 20, 279
BstF5I GGATG 3 cut(s) 514, 664, 756
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 5 cut(s) 16, 118, 345, 829, 974
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 5 cut(s) 13, 115, 342, 826, 971
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 335
BstSNI TACGTA 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 873
BstX2I RGATCY 2 cut(s) 13, 115
BstYI RGATCY 2 cut(s) 13, 115
BstZ17I GTATAC 1 cut(s) 582
BtgI CCRYGG 2 cut(s) 20, 279
BtsCI GGATG 3 cut(s) 514, 664, 756
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 244
CseI GACGC 1 cut(s) 974
CsiI ACCWGGT 1 cut(s) 335
Csp6I GTAC 3 cut(s) 204, 324, 418
CviAII CATG 6 cut(s) 21, 87, 172, 374, 492, 916
CviJI RGCY 5 cut(s) 264, 426, 478, 574, 677
CviKI_1 RGCY 5 cut(s) 264, 426, 478, 574, 677
CviQI GTAC 3 cut(s) 204, 324, 418
DdeI CTNAG 1 cut(s) 848
DpnI GATC 5 cut(s) 15, 117, 344, 828, 973
DpnII GATC 5 cut(s) 13, 115, 342, 826, 971
Eam1104I CTCTTC 3 cut(s) 279, 645, 690
EarI CTCTTC 3 cut(s) 279, 645, 690
Eco105I TACGTA 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 20
Eco32I GATATC 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 335
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 6 cut(s) 24, 90, 175, 377, 495, 919
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FaqI GGGAC 1 cut(s) 747
FatI CATG 6 cut(s) 20, 86, 171, 373, 491, 915
FauI CCCGC 1 cut(s) 64
FblI GTMKAC 2 cut(s) 73, 581
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 3 cut(s) 501, 671, 763
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 2 cut(s) 68, 386
GlaI GCGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 150
HapII CCGG 1 cut(s) 119
HgaI GACGC 1 cut(s) 974
HhaI GCGC 1 cut(s) 244
Hin1I GRCGYC 1 cut(s) 985
Hin1II CATG 6 cut(s) 24, 90, 175, 377, 495, 919
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 1 cut(s) 74
HindII GTYRAC 1 cut(s) 74
HinfI GANTC 4 cut(s) 316, 815, 852, 942
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 4 cut(s) 74, 193, 324, 582
Hpy188I TCNGA 3 cut(s) 333, 849, 1014
Hpy188III TCNNGA 5 cut(s) 48, 119, 374, 436, 656
Hpy8I GTNNAC 4 cut(s) 74, 193, 324, 582
HpyAV CCTTC 1 cut(s) 901
HpyCH4III ACNGT 1 cut(s) 778
HpyCH4IV ACGT 3 cut(s) 446, 522, 715
HpyCH4V TGCA 3 cut(s) 90, 258, 456
HpyF3I CTNAG 1 cut(s) 848
HpySE526I ACGT 3 cut(s) 446, 522, 715
Hsp92I GRCGYC 1 cut(s) 985
Hsp92II CATG 6 cut(s) 24, 90, 175, 377, 495, 919
HspAI GCGC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 118
Kzo9I GATC 5 cut(s) 13, 115, 342, 826, 971
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 1 cut(s) 789
LpnPI CCDG 8 cut(s) 132, 312, 322, 349, 556, 641, 722, 846
Lsp1109I GCAGC 1 cut(s) 161
MabI ACCWGGT 1 cut(s) 335
MaeI CTAG 2 cut(s) 68, 386
MaeII ACGT 3 cut(s) 446, 522, 715
MaeIII GTNAC 4 cut(s) 290, 389, 667, 711
MalI GATC 5 cut(s) 15, 117, 344, 828, 973
MboI GATC 5 cut(s) 13, 115, 342, 826, 971
MboII GAAGA 8 cut(s) 8, 296, 632, 707, 821, 847, 875, 878
MflI RGATCY 2 cut(s) 13, 115
MluCI AATT 8 cut(s) 122, 137, 181, 219, 364, 487, 587, 809
MmeI TCCRAC 1 cut(s) 910
MnlI CCTC 6 cut(s) 280, 432, 489, 536, 648, 714
MroI TCCGGA 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 941
MseI TTAA 1 cut(s) 953
MslI CAYNNNNRTG 1 cut(s) 537
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 1 cut(s) 337
NcoI CCATGG 1 cut(s) 20
NdeII GATC 5 cut(s) 13, 115, 342, 826, 971
NlaIII CATG 6 cut(s) 24, 90, 175, 377, 495, 919
NspI RCATGY 1 cut(s) 90
PagI TCATGA 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 690
PctI GAATGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 941
PfeI GAWTC 4 cut(s) 316, 815, 852, 942
Pfl23II CGTACG 1 cut(s) 417
PkrI GCNGC 1 cut(s) 151
Ppu21I YACGTR 1 cut(s) 447
PsiI TTATAA 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspLI CGTACG 1 cut(s) 417
PsrI GAACNNNNNNTAC 2 cut(s) 905, 937
PsuI RGATCY 2 cut(s) 13, 115
RsaI GTAC 3 cut(s) 205, 325, 419
RsaNI GTAC 3 cut(s) 204, 324, 418
RseI CAYNNNNRTG 1 cut(s) 537
SalI GTCGAC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 1 cut(s) 953
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 5 cut(s) 13, 115, 342, 826, 971
ScrFI CCNGG 1 cut(s) 337
SexAI ACCWGGT 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 537
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
SnaBI TACGTA 1 cut(s) 447
Sse9I AATT 8 cut(s) 122, 137, 181, 219, 364, 487, 587, 809
SsiI CCGC 1 cut(s) 57
SspMI CTAG 2 cut(s) 68, 386
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 778
TaiI ACGT 3 cut(s) 449, 525, 718
TaqI TCGA 5 cut(s) 49, 73, 197, 345, 719
TaqII GACCGA 1 cut(s) 984
TasI AATT 8 cut(s) 122, 137, 181, 219, 364, 487, 587, 809
TatI WGTACW 1 cut(s) 323
TfiI GAWTC 4 cut(s) 316, 815, 852, 942
Tru1I TTAA 1 cut(s) 953
Tru9I TTAA 1 cut(s) 953
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 6 cut(s) 51, 115, 131, 242, 480, 847
TspGWI ACGGA 4 cut(s) 296, 309, 408, 633
XapI RAATTY 4 cut(s) 122, 219, 587, 809
XceI RCATGY 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 73, 581
XmnI GAANNNNTTC 1 cut(s) 941
XspI CTAG 2 cut(s) 68, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.