AT3G22720

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Reverse (-)
8028961 .. 8030100
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22720.1

Sequence Viewer

Length: 1140 bp
ATGATGTCGGATCTTCCTTTAGATTTGGTAGAGAAAATACTCTCTAGGGTTCCCGCCACATCTCTCAAACGACTACGATCGACTTGTAGACGATGGAACGCTTTATTGAAAGACCGAAGATTCACCGAGAAACACTTTCATAAAGCACCAAAGGAGTCTAGAGTTCTCATGTTGAAAGAGTATAGGGTTTTTCCAATAAGCGTCAATCTCAAAGTTACTCCTCCATCTGTAGACTTTAAAGGTGCACTTGGTTATTCCCATTCTAATTCAGAACAAGTCGTGATAACCGAAGTTTTTCACTGCGATGGTTTGTTGTTATGCACCACCAATGACGATAGACTCGTGGTTTGGAATCCGTGTTTGGGGGAAACCAAATGGATCCAACTCAAAGTTGATTACCGGAGATACGTCTCCATATTTGCTCTAGGGTATATCCAAAACAACGAATCCTGCCGTAGCTATAAAATCTTGTGGACTTGGTATAGTTACGATTACAATTCATCACCGCCACAAAGAGGTTTGGGGTTTGAAATATACGAGTTTAGCTCTGATTCATCATGGAGGGTTCTTGATGACGTCAATCACGACTCCGTCGTCAATCACAACTCCATAATAAGAAGTGGCGTGTCTTTGAAGGGAAATACTTATATGCTTGCTTATGATATTGAAGAAAATTCTAAATTTTTACTAATGTTTGATTTTACAACAGAGCGATTGAAACGTCTATGCCTTCCGCATTTCCAAGATGTTGGTGAAATGGATCTATCTGTTAGAGAAGAACAACTCTCAGTATTGAATTGGAGCCGTATTACATCAAAGATGGAGATATGGATAACCAATAACTTTGATACTGAAGCTACATTGTTGTGGAAAAGAGTGCACTTAGACAGTCAATGTAATTGTGTTCGAATCTTCTCAAGTCTATATTTCGACGAGGAGAAGAAAGTCGTCTTGTATTGTAATGTTAAAGATGGTGAGACCAGCAAGAAAGTGGTATACATTATCGGAGAGGACGATGGACATTACATAGAAATCCCTTTCGTAAAATCTATAAAAATAGAAAGGCTTCAACTTATCCATACTACAAGATGGTACTCATTGATTTTCAATTATGTTCCGAGTTTGTTTCAAATCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

379

Amino Acids

44.59

Weight (kDa)

7.57

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 43 5.5e-11 F-box domain
F-box-like PF12937 2 - 40 5.1e-08 F-box-like
FBA_1 PF07734 55 - 378 3.7e-86 F-box associated beta propeller domain
FBA_3 PF08268 94 - 284 3.3e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 593
AatII GACGTC 1 cut(s) 579
AccI GTMKAC 3 cut(s) 88, 231, 996
AciI CCGC 3 cut(s) 54, 506, 734
AclWI GGATC 4 cut(s) 18, 373, 386, 768
AcsI RAATTY 2 cut(s) 673, 680
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 1 cut(s) 1094
AfiI CCNNNNNNNGG 2 cut(s) 362, 515
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 3 cut(s) 459, 546, 857
AluI AGCT 3 cut(s) 459, 546, 857
Alw21I GWGCWC 2 cut(s) 247, 882
Alw26I GTCTC 2 cut(s) 415, 971
Alw44I GTGCAC 2 cut(s) 243, 878
AlwI GGATC 4 cut(s) 18, 373, 386, 768
ApaLI GTGCAC 2 cut(s) 243, 878
ApoI RAATTY 2 cut(s) 673, 680
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
Asp700I GAANNNNTTC 2 cut(s) 294, 1065
AsuHPI GGTGA 4 cut(s) 115, 495, 764, 986
AsuII TTCGAA 1 cut(s) 907
BaeGI GKGCMC 2 cut(s) 247, 882
BamHI GGATCC 1 cut(s) 378
BauI CACGAG 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 247, 882
BccI CCATC 7 cut(s) 87, 232, 299, 814, 965, 1010, 1083
BceAI ACGGC 2 cut(s) 438, 789
BcoDI GTCTC 2 cut(s) 415, 971
BfaI CTAG 3 cut(s) 45, 159, 425
BfmI CTRYAG 1 cut(s) 228
BmiI GGNNCC 3 cut(s) 51, 380, 803
BplI GAGNNNNNCTC 2 cut(s) 530, 562
Bpu14I TTCGAA 1 cut(s) 907
BpuEI CTTGAG 1 cut(s) 901
BsaHI GRCGYC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 971
BsaWI WCCGGW 1 cut(s) 399
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 515
Bse1I ACTGG 1 cut(s) 1135
BseLI CCNNNNNNNGG 2 cut(s) 362, 515
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 1 cut(s) 1135
BseRI GAGGAG 2 cut(s) 210, 950
BseSI GKGCMC 2 cut(s) 247, 882
Bsh1285I CGRYCG 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 247, 882
BsiSI CCGG 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 362, 515
BsmAI GTCTC 2 cut(s) 415, 971
BsmBI CGTCTC 1 cut(s) 415
Bso31I GGTCTC 1 cut(s) 971
Bsp119I TTCGAA 1 cut(s) 907
Bsp1286I GDGCHC 2 cut(s) 247, 882
Bsp143I GATC 4 cut(s) 10, 77, 378, 760
BspACI CCGC 3 cut(s) 54, 506, 734
BspCNI CTCAG 1 cut(s) 800
BspLI GGNNCC 3 cut(s) 51, 380, 803
BspPI GGATC 4 cut(s) 18, 373, 386, 768
BspT104I TTCGAA 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 971
BsrI ACTGG 1 cut(s) 1135
BssMI GATC 4 cut(s) 10, 77, 378, 760
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 1 cut(s) 576
BssSI CACGAG 1 cut(s) 341
Bst1107I GTATAC 1 cut(s) 997
Bst2BI CACGAG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 890
BstACI GRCGYC 1 cut(s) 576
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 787, 883
BstKTI GATC 4 cut(s) 13, 80, 381, 763
BstMAI GTCTC 2 cut(s) 415, 971
BstMBI GATC 4 cut(s) 10, 77, 378, 760
BstMCI CGRYCG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 247, 882
BstX2I RGATCY 3 cut(s) 10, 378, 760
BstXI CCANNNNNNTGG 1 cut(s) 749
BstYI RGATCY 3 cut(s) 10, 378, 760
BstZ17I GTATAC 1 cut(s) 997
BtgZI GCGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 654
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 1093
CviAII CATG 2 cut(s) 169, 558
CviJI RGCY 5 cut(s) 459, 546, 804, 857, 1066
CviKI_1 RGCY 5 cut(s) 459, 546, 804, 857, 1066
CviQI GTAC 1 cut(s) 1093
DdeI CTNAG 2 cut(s) 787, 883
DpnI GATC 4 cut(s) 12, 79, 380, 762
DpnII GATC 4 cut(s) 10, 77, 378, 760
DraI TTTAAA 1 cut(s) 238
DrdI GACNNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 593
Eco31I GGTCTC 1 cut(s) 971
Eco57I CTGAAG 1 cut(s) 873
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 2 cut(s) 172, 561
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FatI CATG 2 cut(s) 168, 557
FauI CCCGC 1 cut(s) 61
FblI GTMKAC 3 cut(s) 88, 231, 996
FspBI CTAG 3 cut(s) 45, 159, 425
HapII CCGG 1 cut(s) 400
HgaI GACGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 2 cut(s) 172, 561
HinfI GANTC 8 cut(s) 120, 155, 339, 352, 446, 551, 587, 909
HpaII CCGG 1 cut(s) 400
HphI GGTGA 4 cut(s) 115, 495, 764, 986
Hpy166II GTNNAC 6 cut(s) 89, 232, 245, 474, 880, 997
Hpy188I TCNGA 5 cut(s) 10, 271, 550, 1007, 1119
Hpy188III TCNNGA 4 cut(s) 159, 280, 569, 584
Hpy8I GTNNAC 6 cut(s) 89, 232, 245, 474, 880, 997
Hpy99I CGWCG 2 cut(s) 596, 935
HpyAV CCTTC 2 cut(s) 628, 740
HpyCH4III ACNGT 1 cut(s) 890
HpyCH4IV ACGT 3 cut(s) 408, 576, 721
HpyCH4V TGCA 3 cut(s) 245, 321, 880
HpyF3I CTNAG 2 cut(s) 787, 883
HpySE526I ACGT 3 cut(s) 408, 576, 721
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 2 cut(s) 172, 561
Kzo9I GATC 4 cut(s) 10, 77, 378, 760
LmnI GCTCC 1 cut(s) 801
LpnPI CCDG 3 cut(s) 413, 463, 994
MaeI CTAG 3 cut(s) 45, 159, 425
MaeII ACGT 3 cut(s) 408, 576, 721
MaeIII GTNAC 2 cut(s) 214, 485
MalI GATC 4 cut(s) 12, 79, 380, 762
MboI GATC 4 cut(s) 10, 77, 378, 760
MboII GAAGA 6 cut(s) 5, 129, 680, 788, 904, 952
MflI RGATCY 3 cut(s) 10, 378, 760
MhlI GDGCHC 2 cut(s) 247, 882
MluCI AATT 7 cut(s) 265, 496, 673, 680, 796, 898, 1108
MlyI GAGTC 3 cut(s) 164, 333, 581
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 5 cut(s) 231, 509, 555, 928, 1003
MroXI GAANNNNTTC 2 cut(s) 294, 1065
MseI TTAA 2 cut(s) 237, 966
MslI CAYNNNNRTG 2 cut(s) 303, 865
MspI CCGG 1 cut(s) 400
NdeII GATC 4 cut(s) 10, 77, 378, 760
NlaIII CATG 2 cut(s) 172, 561
NlaIV GGNNCC 3 cut(s) 51, 380, 803
NspV TTCGAA 1 cut(s) 907
PcsI WCGNNNNNNNCGW 5 cut(s) 285, 339, 582, 591, 1011
PdmI GAANNNNTTC 2 cut(s) 294, 1065
PfeI GAWTC 5 cut(s) 120, 352, 446, 551, 909
PflFI GACNNNGTC 1 cut(s) 590
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 3 cut(s) 163, 333, 581
PpsI GAGTC 3 cut(s) 163, 333, 581
PspN4I GGNNCC 3 cut(s) 51, 380, 803
PsuI RGATCY 3 cut(s) 10, 378, 760
PsyI GACNNNGTC 1 cut(s) 590
PvuI CGATCG 1 cut(s) 80
RsaI GTAC 1 cut(s) 1094
RsaNI GTAC 1 cut(s) 1093
RseI CAYNNNNRTG 2 cut(s) 303, 865
SaqAI TTAA 2 cut(s) 237, 966
Sau3AI GATC 4 cut(s) 10, 77, 378, 760
SchI GAGTC 3 cut(s) 164, 333, 581
SduI GDGCHC 2 cut(s) 247, 882
SetI ASST 8 cut(s) 244, 411, 461, 520, 548, 579, 724, 859
SfcI CTRYAG 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 303, 865
SmlI CTYRAG 1 cut(s) 916
SmoI CTYRAG 1 cut(s) 916
Sse9I AATT 7 cut(s) 265, 496, 673, 680, 796, 898, 1108
SsiI CCGC 3 cut(s) 54, 506, 734
SspMI CTAG 3 cut(s) 45, 159, 425
TaaI ACNGT 1 cut(s) 890
TaiI ACGT 3 cut(s) 411, 579, 724
TaqI TCGA 3 cut(s) 80, 907, 930
TaqII GACCGA 1 cut(s) 129
TasI AATT 7 cut(s) 265, 496, 673, 680, 796, 898, 1108
TfiI GAWTC 5 cut(s) 120, 352, 446, 551, 909
Tru1I TTAA 2 cut(s) 237, 966
Tru9I TTAA 2 cut(s) 237, 966
TscAI CASTG 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 128, 489, 543
TspGWI ACGGA 2 cut(s) 345, 580
TspRI CASTG 1 cut(s) 305
Tth111I GACNNNGTC 1 cut(s) 590
VneI GTGCAC 2 cut(s) 243, 878
XapI RAATTY 2 cut(s) 673, 680
XbaI TCTAGA 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 988
XmiI GTMKAC 3 cut(s) 88, 231, 996
XmnI GAANNNNTTC 2 cut(s) 294, 1065
XspI CTAG 3 cut(s) 45, 159, 425
ZraI GACGTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.