AT5G42460

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000186
Physical Location & Seq
Forward (+)
16979773 .. 16981195
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42460.1

Sequence Viewer

Length: 1167 bp
ATGACGATAATGTCAGATCTTCCACGGGATTTGTTAGCGGAGATTCTCAGTAGGGTTCCGTTGACATCTCTAAGAGCAGTGCGATTGACTTGCAAAAAGTGGAACGATTTATCCAAAGATCGGAGCTTTCTCAAGAAGCAAATCGTCGAAACAAAGAAGAAGCAATTGGAATCGAAGGAGATTGAGGTGATCATGATGAGGAATTTTAGGGTTTATCTAACAAGCATCGATATCCACAACAATGTTGATCCATCATTTACGCCTAAAGGTACACTTATTAGCTTAAGCGATGATGCAAACCATCATCAAGTCGATAATGTATCTAGAGTCTTTCACTGCGATGGTTTACTATTATGCATCACCAAAGACCTCCACTATAGGCTTGTGGTTTGGAACCCTTATTTTGGTCAAACAAGATGGATCCAACCCAGAAATTCTTACCACAGAAAGGACAATTACGCTCTTGGGTACGATGAGAAGAAGAACCACAAAATCCTGAGATTGAAGGATAATTATTACGCTCCACGGGAACGGATTTGTGAGTTTGAACTCTACAGTTTTGAGTCTAATTCATGGAAGGTTGTACTTGATGTCAGTCCTGACTGGTATATACCTTCTTATAACCGTGGCTTGTCTCTAAAGGGGAATACTTACTGGTATGCAACAGAGAAGCATGTCAATGTTGATTTCTTGATTTGTTTCGATTTCACAACAGAGAAATTTGGACCGCGTTTGCCTCTGCCTTTTAACGCTACCGAGTCTCCTACTTATGAAGATGTTGTTACTCTCTCTAGTGTTGGAGAAGAGCAGCTTGCGGTGTTGTTTCAGAGTGAGTATACATTGATGATGGAGATTTGGGTTACGAGCAAGGTTGAGTCCACAGAGGTTTTGTGGAACAAATTGTTCTTAGAGGTTGATTTGATAGCAATCAGCTCTCACTTTCAGTTTCTGGCAGAGGCTGGGAGTTTCTTCATTGACCAGAAGAAGAATGTTGTTGTGGTTTTTGATAAAGATATGGATGAGGCTACCGATCGCGACATGGCTTACGTTGTTGGGAAGAACGGATACTTCAAAAAAGTGGATATTGGGGAAGAAGCATACACGAGTTGTTTCCCACTTGTATGTTCTTATGTTCCAAGCTCAGAGCAAATCAGGCAGCTAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

388

Amino Acids

45.31

Weight (kDa)

5.91

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 45 1.1e-10 F-box domain
F-box-like PF12937 4 - 44 4.1e-09 F-box-like
FBA_1 PF07734 63 - 384 2.7e-111 F-box associated beta propeller domain
FBA_3 PF08268 109 - 295 1.4e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AasI GACNNNNNNGTC 1 cut(s) 10
AccI GTMKAC 1 cut(s) 836
AccII CGCG 2 cut(s) 730, 1035
AciI CCGC 3 cut(s) 38, 728, 815
AclWI GGATC 3 cut(s) 242, 415, 428
AcsI RAATTY 3 cut(s) 202, 433, 719
AfaI GTAC 3 cut(s) 271, 470, 585
AfiI CCNNNNNNNGG 3 cut(s) 120, 404, 448
AflII CTTAAG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 505, 548, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 6 cut(s) 126, 282, 811, 933, 1140, 1159
AluI AGCT 6 cut(s) 126, 282, 811, 933, 1140, 1159
Alw26I GTCTC 2 cut(s) 639, 765
AlwI GGATC 3 cut(s) 242, 415, 428
AlwNI CAGNNNCTG 2 cut(s) 949, 959
ApeKI GCWGC 2 cut(s) 808, 1156
ApoI RAATTY 3 cut(s) 202, 433, 719
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 2 cut(s) 199, 352
AvaII GGWCC 1 cut(s) 725
BamHI GGATCC 1 cut(s) 420
BarI GAAGNNNNNNTAC 2 cut(s) 1049, 1081
BauI CACGAG 1 cut(s) 1102
BbvI GCAGC 1 cut(s) 820
BccI CCATC 5 cut(s) 259, 309, 335, 411, 841
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciVI GTATCC 1 cut(s) 1058
BclI TGATCA 1 cut(s) 189
BcoDI GTCTC 2 cut(s) 639, 765
BfaI CTAG 2 cut(s) 324, 792
BfmI CTRYAG 2 cut(s) 376, 553
BfrI CTTAAG 1 cut(s) 283
BfuI GTATCC 1 cut(s) 1058
BglII AGATCT 1 cut(s) 16
BisI GCNGC 2 cut(s) 809, 1157
BlsI GCNGC 2 cut(s) 810, 1158
Bme18I GGWCC 1 cut(s) 725
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 3 cut(s) 57, 395, 422
BmsI GCATC 3 cut(s) 234, 283, 366
BpuEI CTTGAG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 926
BsaJI CCNNGG 3 cut(s) 23, 524, 625
BsaXI ACNNNNNCTCC 2 cut(s) 745, 775
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 404, 448
Bse1I ACTGG 2 cut(s) 608, 659
Bse8I GATNNNNATC 1 cut(s) 926
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 23, 524, 625
BseGI GGATG 1 cut(s) 1024
BseJI GATNNNNATC 1 cut(s) 926
BseLI CCNNNNNNNGG 3 cut(s) 120, 404, 448
BseMII CTCAG 3 cut(s) 61, 488, 1155
BseNI ACTGG 2 cut(s) 608, 659
BseXI GCAGC 1 cut(s) 820
BseYI CCCAGC 1 cut(s) 959
Bsh1236I CGCG 2 cut(s) 730, 1035
Bsh1285I CGRYCG 1 cut(s) 1033
BshVI ATCGAT 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 1033
BslI CCNNNNNNNGG 3 cut(s) 120, 404, 448
BsmAI GTCTC 2 cut(s) 639, 765
Bsp143I GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
Bsp68I TCGCGA 1 cut(s) 1035
BspACI CCGC 3 cut(s) 38, 728, 815
BspCNI CTCAG 3 cut(s) 60, 489, 1154
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 2 cut(s) 730, 1035
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 57, 395, 422
BspPI GGATC 3 cut(s) 242, 415, 428
BspQI GCTCTTC 1 cut(s) 798
BspTI CTTAAG 1 cut(s) 283
BsrI ACTGG 2 cut(s) 608, 659
BssECI CCNNGG 3 cut(s) 23, 524, 625
BssMI GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
BssNAI GTATAC 1 cut(s) 837
BssSI CACGAG 1 cut(s) 1102
Bst1107I GTATAC 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 1102
Bst4CI ACNGT 2 cut(s) 557, 626
Bst6I CTCTTC 1 cut(s) 798
BstAFI CTTAAG 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 5 cut(s) 47, 71, 497, 907, 1141
BstDSI CCRYGG 3 cut(s) 23, 524, 625
BstF5I GGATG 1 cut(s) 1024
BstFNI CGCG 2 cut(s) 730, 1035
BstKTI GATC 6 cut(s) 19, 121, 192, 250, 423, 1033
BstMAI GTCTC 2 cut(s) 639, 765
BstMBI GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
BstMCI CGRYCG 1 cut(s) 1033
BstMWI GCNNNNNNNGC 1 cut(s) 1153
BstNSI RCATGY 1 cut(s) 677
BstSFI CTRYAG 2 cut(s) 376, 553
BstUI CGCG 2 cut(s) 730, 1035
BstV1I GCAGC 1 cut(s) 820
BstX2I RGATCY 2 cut(s) 16, 420
BstYI RGATCY 2 cut(s) 16, 420
BstZ17I GTATAC 1 cut(s) 837
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 1 cut(s) 1058
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 3 cut(s) 23, 524, 625
BtgZI GCGATG 2 cut(s) 303, 354
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 2 cut(s) 84, 334
BtsIMutI CAGTG 2 cut(s) 84, 334
BtuMI TCGCGA 1 cut(s) 1035
Cac8I GCNNGC 1 cut(s) 813
CaiI CAGNNNCTG 2 cut(s) 949, 959
CciI TCATGA 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 725
ClaI ATCGAT 1 cut(s) 228
Csp6I GTAC 3 cut(s) 270, 469, 584
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 4 cut(s) 193, 573, 674, 1039
CviQI GTAC 3 cut(s) 270, 469, 584
DdeI CTNAG 5 cut(s) 47, 71, 497, 907, 1141
DpnI GATC 6 cut(s) 18, 120, 191, 249, 422, 1032
DpnII GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 725
EcoRV GATATC 1 cut(s) 232
EcoT22I ATGCAT 1 cut(s) 359
FaeI CATG 4 cut(s) 196, 576, 677, 1042
FalI AAGNNNNNCTT 4 cut(s) 258, 290, 795, 827
FatI CATG 4 cut(s) 192, 572, 673, 1038
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 836
Fnu4HI GCNGC 2 cut(s) 809, 1157
FokI GGATG 1 cut(s) 1031
Fsp4HI GCNGC 2 cut(s) 809, 1157
FspBI CTAG 2 cut(s) 324, 792
GluI GCNGC 2 cut(s) 809, 1157
GsaI CCCAGC 1 cut(s) 963
Hin1II CATG 4 cut(s) 196, 576, 677, 1042
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 6 cut(s) 43, 170, 327, 563, 758, 875
HphI GGTGA 2 cut(s) 199, 352
Hpy166II GTNNAC 5 cut(s) 63, 272, 347, 837, 879
Hpy188I TCNGA 4 cut(s) 16, 123, 828, 1144
Hpy188III TCNNGA 7 cut(s) 133, 193, 324, 496, 599, 691, 1034
Hpy8I GTNNAC 5 cut(s) 63, 272, 347, 837, 879
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 4 cut(s) 169, 499, 571, 624
HpyCH4III ACNGT 2 cut(s) 557, 626
HpyCH4IV ACGT 1 cut(s) 1047
HpyCH4V TGCA 4 cut(s) 93, 296, 357, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 1153
HpyF3I CTNAG 5 cut(s) 47, 71, 497, 907, 1141
HpySE526I ACGT 1 cut(s) 1047
Hsp92II CATG 4 cut(s) 196, 576, 677, 1042
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 123, 526
LpnPI CCDG 9 cut(s) 442, 509, 589, 612, 640, 935, 945, 992, 1138
Lsp1109I GCAGC 1 cut(s) 820
LweI GCATC 3 cut(s) 234, 283, 366
MaeI CTAG 2 cut(s) 324, 792
MaeII ACGT 1 cut(s) 1047
MaeIII GTNAC 2 cut(s) 781, 859
MalI GATC 6 cut(s) 18, 120, 191, 249, 422, 1032
MboI GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 2 cut(s) 16, 420
MluCI AATT 8 cut(s) 164, 202, 433, 454, 511, 568, 719, 899
MlyI GAGTC 4 cut(s) 336, 572, 767, 884
MmeI TCCRAC 2 cut(s) 448, 778
MnlI CCTC 8 cut(s) 178, 192, 380, 747, 877, 904, 949, 1015
Mph1103I ATGCAT 1 cut(s) 359
MseI TTAA 2 cut(s) 284, 747
MslI CAYNNNNRTG 4 cut(s) 240, 339, 678, 1120
MspCI CTTAAG 1 cut(s) 283
MunI CAATTG 1 cut(s) 164
MvnI CGCG 2 cut(s) 730, 1035
MwoI GCNNNNNNNGC 1 cut(s) 1153
NdeII GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
NlaIII CATG 4 cut(s) 196, 576, 677, 1042
NlaIV GGNNCC 3 cut(s) 57, 395, 422
NruI TCGCGA 1 cut(s) 1035
NsiI ATGCAT 1 cut(s) 359
NspI RCATGY 1 cut(s) 677
PagI TCATGA 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 798
PfeI GAWTC 2 cut(s) 43, 170
PkrI GCNGC 2 cut(s) 810, 1158
Ple19I CGATCG 1 cut(s) 1033
PleI GAGTC 4 cut(s) 335, 571, 766, 883
PpsI GAGTC 4 cut(s) 335, 571, 766, 883
PsiI TTATAA 1 cut(s) 621
PspFI CCCAGC 1 cut(s) 959
PspN4I GGNNCC 3 cut(s) 57, 395, 422
PspPI GGNCC 1 cut(s) 725
PstNI CAGNNNCTG 2 cut(s) 949, 959
PsuI RGATCY 2 cut(s) 16, 420
PvuI CGATCG 1 cut(s) 1033
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 3 cut(s) 271, 470, 585
RsaNI GTAC 3 cut(s) 270, 469, 584
RseI CAYNNNNRTG 4 cut(s) 240, 339, 678, 1120
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 2 cut(s) 284, 747
SatI GCNGC 2 cut(s) 809, 1157
Sau3AI GATC 6 cut(s) 16, 118, 189, 247, 420, 1030
Sau96I GGNCC 1 cut(s) 725
SchI GAGTC 4 cut(s) 336, 572, 767, 884
SfaNI GCATC 3 cut(s) 234, 283, 366
SfcI CTRYAG 2 cut(s) 376, 553
SinI GGWCC 1 cut(s) 725
SmiMI CAYNNNNRTG 4 cut(s) 240, 339, 678, 1120
SmlI CTYRAG 2 cut(s) 131, 283
SmoI CTYRAG 2 cut(s) 131, 283
Sse9I AATT 8 cut(s) 164, 202, 433, 454, 511, 568, 719, 899
SsiI CCGC 3 cut(s) 38, 728, 815
SspMI CTAG 2 cut(s) 324, 792
TaaI ACNGT 2 cut(s) 557, 626
TaiI ACGT 1 cut(s) 1050
TaqI TCGA 5 cut(s) 147, 173, 228, 312, 702
TasI AATT 8 cut(s) 164, 202, 433, 454, 511, 568, 719, 899
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 2 cut(s) 43, 170
Tru1I TTAA 2 cut(s) 284, 747
Tru9I TTAA 2 cut(s) 284, 747
TscAI CASTG 2 cut(s) 84, 341
TseI GCWGC 2 cut(s) 808, 1156
TspDTI ATGAA 3 cut(s) 561, 786, 961
TspGWI ACGGA 3 cut(s) 48, 547, 1077
TspRI CASTG 2 cut(s) 84, 341
Vha464I CTTAAG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 725
XapI RAATTY 3 cut(s) 202, 433, 719
XbaI TCTAGA 1 cut(s) 323
XceI RCATGY 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 836
XspI CTAG 2 cut(s) 324, 792
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.