AT3G17500

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
5986682 .. 5988498
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G17500.1

Sequence Viewer

Length: 1317 bp
ATGTCAAATCTTCCACTGGATTTAGTAGAGGAAATACTCTCAAGGGTTCCAGCAACATCTTTGAAACGATTACGATCTACTTGCAAATCATGGAACAATTGCTACAAAGACCAAAGATTCACGGAGAAGCACTCTGTTATAGCAGCAAAGCAGTTTCTAGTTCTAATGTTGAAGGATTGTAGGGTTTCCTCAGTGAGCGTCAATCTCAATGAAATTCACAATAACATCGCTGCTCCATCTATAGAGTTGAAGGGTGTAGTTGGGCCACAGATGCAAATATCTGGAATCTTTCACTGCGATGGTTTATTGTTATGCACCACCAAAGACGATAGACTTGAGGTTTGGAACCCTTGTACTGGCCAAACCAGGCGGGTCCAACACAGTATACATTACAAGACTAACTCTGAGTTTGTTCTTGGGTACGTCAACAACAACTCTCGCCATAGCTACAAAATCTTGAGGTACTGGAATTTTTACATGTCTAACTACCGAGTTAGTGAGTTCGAAATCTATGACTTTAGCTCTGATTCATGGAGGTTTATTGATGAAGTTAATCCCTACTGCCTCACAGAAGGAGAGGTGTCTTTGAAAGGAAATACTTACTGGCTAGCTTCCGATGAAAAACGAGATATAGATCTCATACTAAGGTTTGATTTCTCAATAGAAAGATATCAACGCTTGAATCTTCCGATTCTGAAATCTGACTATGAAACTGAAGCTCTATCAGTTGTTAGAGAAAAGCAACTTTCAGTATTACTTAAGAGGAATGATACTCTAGAGAGGGAGATTTGGGTGACGACCAATGACAAGATTGATCACACGACCAAAAACTTGTTGTGGATCAAGTTCTTAGCAATTGATCAACGTACTTGTTATCATTGGTCTTGTGTGAGTTTCTTCATCGACGAGGAGAAGAAAATGGCCGTGTTTTGTGATGGTTATATAGGAGGCATCAATGAGACTAGCAGACGCATGTTCTACATTATTGGAGAGGATAAATATTACAGAGAATTATATGTCGGAGATTCTCCAATAAGATCACACAGGCCATTTGTTTTCAACTACGTTCCAAGTTTGGTTCAAATCCCGCAAGCTACAGTTATACCTAAACGCAGAAGACAACAACTATCATTGGTACTTCAGCGTTGTCGTGCATCAAAGGTGGATGTATGGATAACTGATAAGATTGATACTAAAGAAGTGCAGTGGAGCAAATTCTTTACGGTAGATGTAGACATTGGTGGTCGTGCTATGTTATCGTATGACAGTTTCTTCATCGACAAGGAGAAAGGAATGGCCTTGCTATGTGGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

438

Amino Acids

51.35

Weight (kDa)

8.32

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 1 - 41 1e-09 F-box domain
F-box-like PF12937 1 - 37 3.9e-06 F-box-like
FBA_1 PF07734 54 - 362 5.2e-97 F-box associated beta propeller domain
FBA_3 PF08268 96 - 270 4.4e-13 F-box associated beta propeller domain
FBA_1 PF07734 372 - 436 1.6e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 385, 1233
AciI CCGC 2 cut(s) 370, 1088
AclWI GGATC 1 cut(s) 848
AcoI YGGCCR 2 cut(s) 358, 921
AcsI RAATTY 3 cut(s) 213, 469, 1214
AcuI CTGAAG 2 cut(s) 735, 1124
AfaI GTAC 5 cut(s) 355, 422, 464, 868, 1137
AfiI CCNNNNNNNGG 1 cut(s) 356
AflII CTTAAG 1 cut(s) 758
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 7 cut(s) 64, 172, 250, 589, 682, 1060, 1082
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 5 cut(s) 447, 522, 611, 719, 1094
AluI AGCT 5 cut(s) 447, 522, 611, 719, 1094
Alw26I GTCTC 1 cut(s) 953
AlwI GGATC 1 cut(s) 848
AoxI GGCC 5 cut(s) 263, 358, 921, 1046, 1296
ApeKI GCWGC 2 cut(s) 143, 230
ApoI RAATTY 3 cut(s) 213, 469, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
AspS9I GGNCC 2 cut(s) 263, 373
AsuHPI GGTGA 1 cut(s) 805
AsuII TTCGAA 1 cut(s) 504
AsuNHI GCTAGC 1 cut(s) 607
AvaII GGWCC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 1123
BbvI GCAGC 2 cut(s) 155, 217
BccI CCATC 3 cut(s) 244, 293, 929
BceAI ACGGC 1 cut(s) 908
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 1 cut(s) 367
BclI TGATCA 2 cut(s) 814, 859
BcoDI GTCTC 1 cut(s) 953
BfaI CTAG 4 cut(s) 158, 608, 776, 963
BfmI CTRYAG 2 cut(s) 240, 1095
BfrI CTTAAG 1 cut(s) 758
BglII AGATCT 1 cut(s) 634
BisI GCNGC 2 cut(s) 144, 231
BlsI GCNGC 2 cut(s) 145, 232
Bme1390I CCNGG 1 cut(s) 367
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 263, 373
BmiI GGNNCC 3 cut(s) 48, 347, 374
BmrFI CCNGG 1 cut(s) 367
BmsI GCATC 3 cut(s) 261, 960, 1163
BmtI GCTAGC 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 1123
BplI GAGNNNNNCTC 2 cut(s) 116, 148
Bpu14I TTCGAA 1 cut(s) 504
BpuEI CTTGAG 3 cut(s) 25, 356, 478
BsaBI GATNNNNATC 2 cut(s) 73, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 4 cut(s) 21, 361, 470, 608
Bse8I GATNNNNATC 2 cut(s) 73, 633
BseBI CCWGG 1 cut(s) 367
BseGI GGATG 1 cut(s) 1171
BseJI GATNNNNATC 2 cut(s) 73, 633
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 204, 396
BseNI ACTGG 4 cut(s) 21, 361, 470, 608
BseRI GAGGAG 1 cut(s) 923
BseXI GCAGC 2 cut(s) 155, 217
BsgI GTGCAG 1 cut(s) 1223
BshFI GGCC 5 cut(s) 265, 360, 923, 1048, 1298
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 953
BsnI GGCC 5 cut(s) 265, 360, 923, 1048, 1298
Bsp119I TTCGAA 1 cut(s) 504
Bsp143I GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
BspACI CCGC 2 cut(s) 370, 1088
BspANI GGCC 5 cut(s) 265, 360, 923, 1048, 1298
BspCNI CTCAG 2 cut(s) 203, 397
BspLI GGNNCC 3 cut(s) 48, 347, 374
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 1 cut(s) 848
BspT104I TTCGAA 1 cut(s) 504
BspTI CTTAAG 1 cut(s) 758
BsrI ACTGG 4 cut(s) 21, 361, 470, 608
BssMI GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
BssNAI GTATAC 1 cut(s) 386
Bst1107I GTATAC 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 4 cut(s) 383, 1099, 1225, 1268
BstAFI CTTAAG 1 cut(s) 758
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 609, 1092
BstDEI CTNAG 4 cut(s) 190, 405, 644, 850
BstF5I GGATG 1 cut(s) 1171
BstKTI GATC 6 cut(s) 77, 637, 817, 843, 862, 1040
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 2 cut(s) 481, 976
BstSCI CCNGG 1 cut(s) 365
BstSFI CTRYAG 2 cut(s) 240, 1095
BstV1I GCAGC 2 cut(s) 155, 217
BstV2I GAAGAC 1 cut(s) 1123
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BstZ17I GTATAC 1 cut(s) 386
BsuRI GGCC 5 cut(s) 265, 360, 923, 1048, 1298
BtgZI GCGATG 2 cut(s) 211, 312
BtsCI GGATG 1 cut(s) 1171
BtsI GCAGTG 2 cut(s) 292, 1211
BtsIMutI CAGTG 4 cut(s) 14, 198, 292, 1211
Cac8I GCNNGC 2 cut(s) 609, 1092
Cfr13I GGNCC 2 cut(s) 263, 373
CseI GACGC 2 cut(s) 187, 978
Csp6I GTAC 5 cut(s) 354, 421, 463, 867, 1136
CviAII CATG 4 cut(s) 90, 478, 531, 973
CviQI GTAC 5 cut(s) 354, 421, 463, 867, 1136
DdeI CTNAG 4 cut(s) 190, 405, 644, 850
DpnI GATC 6 cut(s) 76, 636, 816, 842, 861, 1039
DpnII GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
EaeI YGGCCR 2 cut(s) 358, 921
Eco32I GATATC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 735, 1124
EcoRII CCWGG 1 cut(s) 365
EcoRV GATATC 1 cut(s) 671
FaeI CATG 4 cut(s) 93, 481, 534, 976
FatI CATG 4 cut(s) 89, 477, 530, 972
FauI CCCGC 2 cut(s) 363, 1095
FbaI TGATCA 2 cut(s) 814, 859
FblI GTMKAC 2 cut(s) 385, 1233
Fnu4HI GCNGC 2 cut(s) 144, 231
FokI GGATG 1 cut(s) 1178
Fsp4HI GCNGC 2 cut(s) 144, 231
FspBI CTAG 4 cut(s) 158, 608, 776, 963
GluI GCNGC 2 cut(s) 144, 231
HaeIII GGCC 5 cut(s) 265, 360, 923, 1048, 1298
HgaI GACGC 2 cut(s) 187, 978
Hin1II CATG 4 cut(s) 93, 481, 534, 976
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 6 cut(s) 117, 285, 527, 682, 691, 1025
HphI GGTGA 1 cut(s) 805
Hpy166II GTNNAC 3 cut(s) 386, 427, 1234
Hpy188I TCNGA 7 cut(s) 406, 526, 616, 690, 696, 703, 1022
Hpy188III TCNNGA 3 cut(s) 282, 457, 776
Hpy8I GTNNAC 3 cut(s) 386, 427, 1234
Hpy99I CGWCG 1 cut(s) 908
HpyAV CCTTC 3 cut(s) 166, 244, 566
HpyCH4III ACNGT 4 cut(s) 383, 1099, 1225, 1268
HpyCH4IV ACGT 3 cut(s) 423, 865, 1065
HpyCH4V TGCA 5 cut(s) 84, 274, 315, 1154, 1204
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 4 cut(s) 190, 405, 644, 850
HpySE526I ACGT 3 cut(s) 423, 865, 1065
Hsp92II CATG 4 cut(s) 93, 481, 534, 976
Ksp22I TGATCA 2 cut(s) 814, 859
Kzo9I GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
LmnI GCTCC 2 cut(s) 238, 1209
LpnPI CCDG 9 cut(s) 2, 63, 267, 342, 352, 379, 451, 589, 1030
Lsp1109I GCAGC 2 cut(s) 155, 217
LweI GCATC 3 cut(s) 261, 960, 1163
MaeI CTAG 4 cut(s) 158, 608, 776, 963
MaeII ACGT 3 cut(s) 423, 865, 1065
MaeIII GTNAC 1 cut(s) 793
MalI GATC 6 cut(s) 76, 636, 816, 842, 861, 1039
MboI GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
MboII GAAGA 5 cut(s) 677, 889, 925, 1128, 1264
MfeI CAATTG 2 cut(s) 97, 855
MflI RGATCY 1 cut(s) 634
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 6 cut(s) 97, 213, 469, 855, 1010, 1214
MluNI TGGCCA 1 cut(s) 360
MmeI TCCRAC 2 cut(s) 400, 1000
Mox20I TGGCCA 1 cut(s) 360
MscI TGGCCA 1 cut(s) 360
MseI TTAA 2 cut(s) 552, 759
MslI CAYNNNNRTG 1 cut(s) 297
Msp20I TGGCCA 1 cut(s) 360
MspCI CTTAAG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 367
MunI CAATTG 2 cut(s) 97, 855
MvaI CCWGG 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 271
NdeII GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
NheI GCTAGC 1 cut(s) 607
NlaIII CATG 4 cut(s) 93, 481, 534, 976
NlaIV GGNNCC 3 cut(s) 48, 347, 374
NmuCI GTSAC 1 cut(s) 793
NspI RCATGY 2 cut(s) 481, 976
NspV TTCGAA 1 cut(s) 504
PciI ACATGT 1 cut(s) 477
PfeI GAWTC 6 cut(s) 117, 285, 527, 682, 691, 1025
PkrI GCNGC 2 cut(s) 145, 232
PscI ACATGT 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 365
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 48, 347, 374
PspPI GGNCC 2 cut(s) 263, 373
PsrI GAACNNNNNNTAC 2 cut(s) 86, 118
PsuI RGATCY 1 cut(s) 634
RsaI GTAC 5 cut(s) 355, 422, 464, 868, 1137
RsaNI GTAC 5 cut(s) 354, 421, 463, 867, 1136
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 2 cut(s) 552, 759
SatI GCNGC 2 cut(s) 144, 231
Sau3AI GATC 6 cut(s) 74, 634, 814, 840, 859, 1037
Sau96I GGNCC 2 cut(s) 263, 373
ScrFI CCNGG 1 cut(s) 367
SfaNI GCATC 3 cut(s) 261, 960, 1163
SfcI CTRYAG 2 cut(s) 240, 1095
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 4 cut(s) 40, 335, 457, 758
SmoI CTYRAG 4 cut(s) 40, 335, 457, 758
Sse9I AATT 6 cut(s) 97, 213, 469, 855, 1010, 1214
SsiI CCGC 2 cut(s) 370, 1088
SspI AATATT 1 cut(s) 1001
SspMI CTAG 4 cut(s) 158, 608, 776, 963
StyD4I CCNGG 1 cut(s) 365
TaaI ACNGT 4 cut(s) 383, 1099, 1225, 1268
TaiI ACGT 3 cut(s) 426, 868, 1068
TaqI TCGA 3 cut(s) 504, 903, 1278
TasI AATT 6 cut(s) 97, 213, 469, 855, 1010, 1214
TatI WGTACW 1 cut(s) 353
TfiI GAWTC 6 cut(s) 117, 285, 527, 682, 691, 1025
Tru1I TTAA 2 cut(s) 552, 759
Tru9I TTAA 2 cut(s) 552, 759
TscAI CASTG 4 cut(s) 21, 198, 299, 1211
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 2 cut(s) 143, 230
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 7 cut(s) 225, 519, 561, 633, 723, 889, 1264
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 4 cut(s) 21, 198, 299, 1211
Vha464I CTTAAG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 3 cut(s) 213, 469, 1214
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 2 cut(s) 481, 976
XmiI GTMKAC 2 cut(s) 385, 1233
XspI CTAG 4 cut(s) 158, 608, 776, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.