AT3G24700

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
9022281 .. 9023453
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24700.2

Sequence Viewer

Length: 750 bp
ATGCTTACTGATCTTCCACTTGATTTAGAATCGGAAATCCTCTCTAGGGTTCCGGCCACTTCTCTACAAAGACTGAAAACGACTTGCAAACGTTGGTACGCTTTATTCAGAGATCCAAGATTCGTCAAGAAAAACTTGGGTAAAGCAGCAACGCATGTGATCTTTGATAATCGTAGTGGTTATTCAATGACAGATATAAATTCTCTGATTCATAGCATCAATCTCCGTGGAATCCAAAACAGCTTTGATCCATCCATTGGGGTCGACGTTAAACTTAACGTTCTAAAGGATCCAAGACATGATAAGATAAGTCATATTATCTCTCACTGCGATGGTTTATTATTGTGCAAGACCGAGGACTATGGTAGACTCGTGGTTTGGAACCCTTGCACTGGTCAAATCAAGTGGATCCAAGCCAATAATATGTTAATGGACGTTTATGTGCTAGGATACGTAAACAACAACAAATCTTGCAATAGCTACAAAATCTTGAATTTTGGCATTCTTCCATTAAACAGTTCTCATGATAATAAGTCTAAAATCTATGAGTTCAACTCTGATTCATGGAGAATTCTTGACCACGTCTCTCCCGGTTACTTCGCAATATCCAAAGCCATGACTTTGAAGGGAAATGCTTATTGGTTTGCTTCAGATTGGTATGCTTCAGATAAAGAAAGAAAATTCTTACACAAATTTGATTTCAGAAGCGAGAGATTTGGACGTCTATGTATTCCTATTCAGAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

249

Amino Acids

28.82

Weight (kDa)

9.29

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 43 3.9e-09 F-box domain
F-box-like PF12937 3 - 41 7.9e-07 F-box-like
FBA_1 PF07734 63 - 220 3e-42 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 724
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 2 cut(s) 264, 367
AclI AACGTT 2 cut(s) 91, 279
AclWI GGATC 6 cut(s) 107, 242, 284, 297, 403, 416
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 5 cut(s) 199, 493, 570, 680, 692
AcuI CTGAAG 2 cut(s) 633, 648
AcyI GRCGYC 1 cut(s) 721
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 3 cut(s) 46, 257, 392
AgsI TTSAA 4 cut(s) 186, 493, 553, 625
AjiI CACGTC 1 cut(s) 583
AjuI GAANNNNNNNTTGG 2 cut(s) 622, 654
AluBI AGCT 2 cut(s) 243, 480
AluI AGCT 2 cut(s) 243, 480
Alw26I GTCTC 1 cut(s) 589
AlwI GGATC 6 cut(s) 107, 242, 284, 297, 403, 416
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 5 cut(s) 199, 493, 570, 680, 692
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
AsuC2I CCSGG 1 cut(s) 591
BamHI GGATCC 2 cut(s) 289, 408
BauI CACGAG 1 cut(s) 371
BbvI GCAGC 1 cut(s) 158
BccI CCATC 2 cut(s) 259, 326
BciVI GTATCC 1 cut(s) 443
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 2 cut(s) 45, 446
BfuI GTATCC 1 cut(s) 443
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 591
BmgBI CACGTC 1 cut(s) 583
BmiI GGNNCC 4 cut(s) 51, 291, 383, 410
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpuMI CCSGG 1 cut(s) 591
BsaAI YACGTR 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 721
BsaJI CCNNGG 2 cut(s) 226, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 257, 392
Bse1I ACTGG 1 cut(s) 397
BseDI CCNNGG 2 cut(s) 226, 354
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 3 cut(s) 46, 257, 392
BseNI ACTGG 1 cut(s) 397
BseXI GCAGC 1 cut(s) 158
BshFI GGCC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 53, 591
BslI CCNNNNNNNGG 3 cut(s) 46, 257, 392
BsmAI GTCTC 1 cut(s) 589
BsmBI CGTCTC 1 cut(s) 589
BsmI GAATGC 1 cut(s) 501
BsnI GGCC 1 cut(s) 56
Bsp143I GATC 6 cut(s) 10, 112, 159, 247, 289, 408
BspANI GGCC 1 cut(s) 56
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 4 cut(s) 51, 291, 383, 410
BspPI GGATC 6 cut(s) 107, 242, 284, 297, 403, 416
BsrI ACTGG 1 cut(s) 397
BssECI CCNNGG 2 cut(s) 226, 354
BssMI GATC 6 cut(s) 10, 112, 159, 247, 289, 408
BssNI GRCGYC 1 cut(s) 721
BssSI CACGAG 1 cut(s) 371
Bst2BI CACGAG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 518
BstACI GRCGYC 1 cut(s) 721
BstBAI YACGTR 1 cut(s) 454
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 251
BstKTI GATC 6 cut(s) 13, 115, 162, 250, 292, 411
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 6 cut(s) 10, 112, 159, 247, 289, 408
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 589
BstSNI TACGTA 1 cut(s) 454
BstV1I GCAGC 1 cut(s) 158
BstX2I RGATCY 3 cut(s) 112, 289, 408
BstYI RGATCY 3 cut(s) 112, 289, 408
BsuI GTATCC 1 cut(s) 443
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 1 cut(s) 251
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 325, 390
CciI TCATGA 1 cut(s) 523
Csp6I GTAC 1 cut(s) 97
CspCI CAANNNNNGTGG 4 cut(s) 208, 243, 386, 421
CviAII CATG 5 cut(s) 155, 299, 524, 564, 616
CviJI RGCY 5 cut(s) 56, 243, 416, 480, 614
CviKI_1 RGCY 5 cut(s) 56, 243, 416, 480, 614
CviQI GTAC 1 cut(s) 97
DpnI GATC 6 cut(s) 12, 114, 161, 249, 291, 410
DpnII GATC 6 cut(s) 10, 112, 159, 247, 289, 408
EaeI YGGCCR 1 cut(s) 54
Eco105I TACGTA 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 633, 648
EcoRI GAATTC 1 cut(s) 570
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 5 cut(s) 158, 302, 527, 567, 619
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FatI CATG 5 cut(s) 154, 298, 523, 563, 615
FblI GTMKAC 2 cut(s) 264, 367
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 2 cut(s) 45, 446
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 1 cut(s) 56
HapII CCGG 2 cut(s) 53, 591
Hin1I GRCGYC 1 cut(s) 721
Hin1II CATG 5 cut(s) 158, 302, 527, 567, 619
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 6 cut(s) 29, 120, 208, 231, 369, 560
HpaII CCGG 2 cut(s) 53, 591
Hpy166II GTNNAC 3 cut(s) 265, 368, 457
Hpy188I TCNGA 8 cut(s) 34, 110, 207, 559, 652, 667, 704, 741
Hpy188III TCNNGA 4 cut(s) 127, 490, 524, 575
Hpy8I GTNNAC 3 cut(s) 265, 368, 457
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 619
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 7 cut(s) 91, 267, 279, 435, 453, 582, 721
HpyCH4V TGCA 4 cut(s) 87, 348, 390, 474
HpySE526I ACGT 7 cut(s) 91, 267, 279, 435, 453, 582, 721
Hsp92I GRCGYC 1 cut(s) 721
Hsp92II CATG 5 cut(s) 158, 302, 527, 567, 619
Kzo9I GATC 6 cut(s) 10, 112, 159, 247, 289, 408
LpnPI CCDG 3 cut(s) 66, 378, 604
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 45, 446
MaeII ACGT 7 cut(s) 91, 267, 279, 435, 453, 582, 721
MaeIII GTNAC 1 cut(s) 593
MalI GATC 6 cut(s) 12, 114, 161, 249, 291, 410
MboI GATC 6 cut(s) 10, 112, 159, 247, 289, 408
MboII GAAGA 2 cut(s) 5, 497
MflI RGATCY 3 cut(s) 112, 289, 408
MluCI AATT 5 cut(s) 199, 493, 570, 680, 692
MlyI GAGTC 1 cut(s) 363
MnlI CCTC 2 cut(s) 50, 349
MseI TTAA 4 cut(s) 270, 276, 428, 512
MslI CAYNNNNRTG 1 cut(s) 330
MspI CCGG 2 cut(s) 53, 591
MspR9I CCNGG 1 cut(s) 591
Mva1269I GAATGC 1 cut(s) 501
NciI CCSGG 1 cut(s) 591
NdeII GATC 6 cut(s) 10, 112, 159, 247, 289, 408
NlaIII CATG 5 cut(s) 158, 302, 527, 567, 619
NlaIV GGNNCC 4 cut(s) 51, 291, 383, 410
NspI RCATGY 1 cut(s) 158
PagI TCATGA 1 cut(s) 523
PctI GAATGC 1 cut(s) 501
PfeI GAWTC 5 cut(s) 29, 120, 208, 231, 560
PflFI GACNNNGTC 1 cut(s) 581
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
Ppu21I YACGTR 1 cut(s) 454
Psp1406I AACGTT 2 cut(s) 91, 279
PspN4I GGNNCC 4 cut(s) 51, 291, 383, 410
PsuI RGATCY 3 cut(s) 112, 289, 408
PsyI GACNNNGTC 1 cut(s) 581
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 330
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 270, 276, 428, 512
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 6 cut(s) 10, 112, 159, 247, 289, 408
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 591
SetI ASST 9 cut(s) 94, 245, 270, 282, 438, 456, 482, 585, 724
SfaNI GCATC 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 330
SnaBI TACGTA 1 cut(s) 454
Sse9I AATT 5 cut(s) 199, 493, 570, 680, 692
SspI AATATT 1 cut(s) 746
SspMI CTAG 2 cut(s) 45, 446
StyD4I CCNGG 1 cut(s) 589
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 7 cut(s) 94, 270, 282, 438, 456, 585, 724
TaqI TCGA 1 cut(s) 264
TaqII GACCGA 1 cut(s) 368
TasI AATT 5 cut(s) 199, 493, 570, 680, 692
TfiI GAWTC 5 cut(s) 29, 120, 208, 231, 560
Tru1I TTAA 4 cut(s) 270, 276, 428, 512
Tru9I TTAA 4 cut(s) 270, 276, 428, 512
TscAI CASTG 2 cut(s) 332, 397
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 200, 552
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 2 cut(s) 332, 397
Tth111I GACNNNGTC 1 cut(s) 581
Van91I CCANNNNNTGG 1 cut(s) 257
XapI RAATTY 5 cut(s) 199, 493, 570, 680, 692
XceI RCATGY 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 264, 367
XspI CTAG 2 cut(s) 45, 446
ZraI GACGTC 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.