AT3G22350

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Forward (+)
7901905 .. 7903041
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22350.1

Sequence Viewer

Length: 1137 bp
ATGTCTGATCTTCCTTTGGATTTGGTAGAAGAAATACTCTCAAGGGTTTCGGCCACATCTCTGAAACGATTACGATCTACTTGCAAACAATGGAACACTTTATTCAAAAAACGAAGTTTCAGCCAGAAACACTTTCATATAGCTCCAAAGGAGTCTATGGTTCTCATGTTGAAGGAGTACAGAGTTTGCTCAATGAATATCAATCTCAATGTTTCTCCTCCATCTGTAGAGTTTCAAGGTACACTTGGCATAAAAGATGATTCCCATTCTAATTTAGGACAAGTCGAGATAGTCGAAGTTTATCACTGTGATGGTTTGTTGTTATGCGCCACCAGGGACAATAGACTCGTGGTTTGGAATCCGTGTTTGGGGGAAACCAGGTGGATCCAACTCAAAGATGAATGCAGGAGATACTCTACGTTTGCTCTAGGATACGAAAACAACAAATTTTGTCGTCGTAACTACAAAATCTTGAGGTATTGGGGTTGGTTTCACGATCACATACCAGACGACGGCGGACGTTTCAGGTTTGAAATATATGACTTTAGATCTGATTCATGGAAGGTTCTTGATGACGTCCCTGATGACCGCTTCCCACCAGTAAGTGTCGTGTCTTTAAAGGGAAACACTTACTGGTTTGGTTCTAATAAAAAGGACTTCTTACGTAGTTTTGATTTTACAACAGAGAGATTTAATCATATTGGTCTTCCGCCATCTCGAGATCATGGTTTTATGGCTCTATCAGTTGTTGGAGAAGAACAACTCTCAGTGTTACAAGAGATTGAGACATCAAAGATGGAGATATTTGTAACCAATAAAGTTGGTACTGAAGAAGCTGCTTTGTTGTGGAGCAAATCCTTTATAGTGGATTTACCCATTGGCGGTCATTGTTCTGAGGTTTTCGCGAGTCTTTTAATCAACGAGGAGAAGAAAGTGGCCTTGTGTTGTAATTTAGATTTCGAGACCGACGGGAACTTGATATTCACTATTGGAGAAGACAATAAATATTACTCAGAAATCCCTTATGCTACAAACAAGCCATGGTGGTTATGTAGATTTTCTGAAAAATTTACAAAAAAATGGTGGTGGTTTCCATTTATTTACAATTATGTTCCAAGTTTGGTTCAAATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

378

Amino Acids

44.25

Weight (kDa)

6.34

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 1 - 40 3e-10 F-box domain
F-box-like PF12937 1 - 39 1.1e-06 F-box-like
FBA_1 PF07734 53 - 377 6.6e-92 F-box associated beta propeller domain
FBA_3 PF08268 85 - 286 3.3e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 579
AccII CGCG 1 cut(s) 905
AciI CCGC 4 cut(s) 516, 589, 710, 882
AclWI GGATC 2 cut(s) 379, 392
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 446, 1067
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 3 cut(s) 179, 241, 826
AfiI CCNNNNNNNGG 3 cut(s) 368, 512, 881
AgsI TTSAA 5 cut(s) 106, 172, 236, 533, 1127
AjnI CCWGG 2 cut(s) 332, 377
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 2 cut(s) 143, 836
AluI AGCT 2 cut(s) 143, 836
Alw26I GTCTC 2 cut(s) 779, 956
AlwI GGATC 2 cut(s) 379, 392
Ama87I CYCGRG 1 cut(s) 717
AoxI GGCC 2 cut(s) 51, 936
ApeKI GCWGC 1 cut(s) 836
ApoI RAATTY 2 cut(s) 446, 1067
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
AspLEI GCGC 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 717
BamHI GGATCC 1 cut(s) 384
BauI CACGAG 1 cut(s) 347
BbsI GAAGAC 2 cut(s) 698, 1002
BbvI GCAGC 1 cut(s) 823
BccI CCATC 4 cut(s) 229, 305, 721, 790
BceAI ACGGC 1 cut(s) 529
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 2 cut(s) 334, 379
BciVI GTATCC 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 779, 956
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 1 cut(s) 548
BisI GCNGC 1 cut(s) 837
BlsI GCNGC 1 cut(s) 838
Bme1390I CCNGG 2 cut(s) 334, 379
BmeT110I CYCGRG 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 386
BmrFI CCNGG 2 cut(s) 334, 379
BpiI GAAGAC 2 cut(s) 698, 1002
BpuEI CTTGAG 2 cut(s) 25, 493
BsaAI YACGTR 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 2 cut(s) 333, 1040
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 512, 881
Bse1I ACTGG 2 cut(s) 599, 638
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 2 cut(s) 334, 379
BseDI CCNNGG 2 cut(s) 333, 1040
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 368, 512, 881
BseMII CTCAG 3 cut(s) 780, 885, 1026
BseNI ACTGG 2 cut(s) 599, 638
BseRI GAGGAG 2 cut(s) 207, 938
BseXI GCAGC 1 cut(s) 823
Bsh1236I CGCG 1 cut(s) 905
BshFI GGCC 2 cut(s) 53, 938
BsiHKCI CYCGRG 1 cut(s) 717
BslFI GGGAC 2 cut(s) 350, 563
BslI CCNNNNNNNGG 3 cut(s) 368, 512, 881
BsmAI GTCTC 2 cut(s) 779, 956
BsmFI GGGAC 2 cut(s) 350, 563
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 2 cut(s) 53, 938
Bso31I GGTCTC 1 cut(s) 956
BsoBI CYCGRG 1 cut(s) 717
Bsp143I GATC 6 cut(s) 7, 74, 384, 496, 548, 721
Bsp19I CCATGG 1 cut(s) 1040
Bsp68I TCGCGA 1 cut(s) 905
BspACI CCGC 4 cut(s) 516, 589, 710, 882
BspANI GGCC 2 cut(s) 53, 938
BspCNI CTCAG 3 cut(s) 779, 886, 1025
BspFNI CGCG 1 cut(s) 905
BspLI GGNNCC 1 cut(s) 386
BspPI GGATC 2 cut(s) 379, 392
BspTNI GGTCTC 1 cut(s) 956
BsrI ACTGG 2 cut(s) 599, 638
BssECI CCNNGG 2 cut(s) 333, 1040
BssMI GATC 6 cut(s) 7, 74, 384, 496, 548, 721
BssNI GRCGYC 1 cut(s) 576
BssSI CACGAG 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 1040
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 2 cut(s) 334, 379
Bst4CI ACNGT 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 665
BstDEI CTNAG 3 cut(s) 766, 894, 1012
BstDSI CCRYGG 1 cut(s) 1040
BstFNI CGCG 1 cut(s) 905
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 6 cut(s) 10, 77, 387, 499, 551, 724
BstMAI GTCTC 2 cut(s) 779, 956
BstMBI GATC 6 cut(s) 7, 74, 384, 496, 548, 721
BstNI CCWGG 2 cut(s) 334, 379
BstSCI CCNGG 2 cut(s) 332, 377
BstSFI CTRYAG 1 cut(s) 225
BstSNI TACGTA 1 cut(s) 665
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 1 cut(s) 823
BstV2I GAAGAC 2 cut(s) 698, 1002
BstX2I RGATCY 2 cut(s) 384, 548
BstYI RGATCY 2 cut(s) 384, 548
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 53, 938
BtgI CCRYGG 1 cut(s) 1040
BtsIMutI CAGTG 2 cut(s) 304, 774
BtuMI TCGCGA 1 cut(s) 905
CfoI GCGC 1 cut(s) 329
CsiI ACCWGGT 1 cut(s) 377
Csp6I GTAC 3 cut(s) 178, 240, 825
CviAII CATG 4 cut(s) 166, 558, 725, 1041
CviJI RGCY 7 cut(s) 53, 123, 143, 737, 836, 938, 1039
CviKI_1 RGCY 7 cut(s) 53, 123, 143, 737, 836, 938, 1039
CviQI GTAC 3 cut(s) 178, 240, 825
DdeI CTNAG 3 cut(s) 766, 894, 1012
DpnI GATC 6 cut(s) 9, 76, 386, 498, 550, 723
DpnII GATC 6 cut(s) 7, 74, 384, 496, 548, 721
DraI TTTAAA 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 2 cut(s) 531, 699
Eco105I TACGTA 1 cut(s) 665
Eco130I CCWWGG 1 cut(s) 1040
Eco31I GGTCTC 1 cut(s) 956
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 717
EcoRII CCWGG 2 cut(s) 332, 377
EcoT14I CCWWGG 1 cut(s) 1040
ErhI CCWWGG 1 cut(s) 1040
FaeI CATG 4 cut(s) 169, 561, 728, 1044
FalI AAGNNNNNCTT 4 cut(s) 228, 260, 644, 676
FaqI GGGAC 2 cut(s) 350, 563
FatI CATG 4 cut(s) 165, 557, 724, 1040
Fnu4HI GCNGC 1 cut(s) 837
Fsp4HI GCNGC 1 cut(s) 837
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 328
GluI GCNGC 1 cut(s) 837
HaeIII GGCC 2 cut(s) 53, 938
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 4 cut(s) 169, 561, 728, 1044
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HinfI GANTC 6 cut(s) 152, 260, 345, 358, 554, 907
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 6 cut(s) 7, 63, 553, 895, 1015, 1063
Hpy188III TCNNGA 8 cut(s) 286, 472, 494, 569, 717, 719, 904, 961
Hpy8I GTNNAC 1 cut(s) 242
Hpy99I CGWCG 3 cut(s) 459, 515, 971
HpyAV CCTTC 2 cut(s) 166, 556
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 4 cut(s) 419, 520, 576, 664
HpyCH4V TGCA 2 cut(s) 84, 405
HpyF3I CTNAG 3 cut(s) 766, 894, 1012
HpySE526I ACGT 4 cut(s) 419, 520, 576, 664
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 4 cut(s) 169, 561, 728, 1044
HspAI GCGC 1 cut(s) 327
Kzo9I GATC 6 cut(s) 7, 74, 384, 496, 548, 721
LmnI GCTCC 2 cut(s) 148, 849
Lsp1109I GCAGC 1 cut(s) 823
MabI ACCWGGT 1 cut(s) 377
MaeI CTAG 1 cut(s) 428
MaeII ACGT 4 cut(s) 419, 520, 576, 664
MaeIII GTNAC 3 cut(s) 458, 771, 808
MalI GATC 6 cut(s) 9, 76, 386, 498, 550, 723
MboI GATC 6 cut(s) 7, 74, 384, 496, 548, 721
MboII GAAGA 6 cut(s) 41, 698, 767, 842, 940, 1007
MflI RGATCY 2 cut(s) 384, 548
MluCI AATT 5 cut(s) 271, 446, 949, 1067, 1105
MlyI GAGTC 3 cut(s) 161, 339, 916
MmeI TCCRAC 2 cut(s) 412, 730
MnlI CCTC 4 cut(s) 228, 468, 889, 916
MseI TTAA 3 cut(s) 617, 693, 914
MslI CAYNNNNRTG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 334, 379
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 2 cut(s) 334, 379
MvnI CGCG 1 cut(s) 905
NcoI CCATGG 1 cut(s) 1040
NdeII GATC 6 cut(s) 7, 74, 384, 496, 548, 721
NlaIII CATG 4 cut(s) 169, 561, 728, 1044
NlaIV GGNNCC 1 cut(s) 386
NruI TCGCGA 1 cut(s) 905
PaeR7I CTCGAG 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 3 cut(s) 260, 358, 554
PkrI GCNGC 1 cut(s) 838
PleI GAGTC 3 cut(s) 160, 339, 915
PpsI GAGTC 3 cut(s) 160, 339, 915
Ppu21I YACGTR 1 cut(s) 665
Psp6I CCWGG 2 cut(s) 332, 377
PspGI CCWGG 2 cut(s) 332, 377
PspN4I GGNNCC 1 cut(s) 386
PsuI RGATCY 2 cut(s) 384, 548
RruI TCGCGA 1 cut(s) 905
RsaI GTAC 3 cut(s) 179, 241, 826
RsaNI GTAC 3 cut(s) 178, 240, 825
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 3 cut(s) 617, 693, 914
SatI GCNGC 1 cut(s) 837
Sau3AI GATC 6 cut(s) 7, 74, 384, 496, 548, 721
SchI GAGTC 3 cut(s) 161, 339, 916
ScrFI CCNGG 2 cut(s) 334, 379
SexAI ACCWGGT 1 cut(s) 377
SfcI CTRYAG 1 cut(s) 225
Sfr274I CTCGAG 1 cut(s) 717
SlaI CTCGAG 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 3 cut(s) 40, 472, 717
SmoI CTYRAG 3 cut(s) 40, 472, 717
SnaBI TACGTA 1 cut(s) 665
Sse9I AATT 5 cut(s) 271, 446, 949, 1067, 1105
SsiI CCGC 4 cut(s) 516, 589, 710, 882
SspI AATATT 1 cut(s) 1007
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 332, 377
StyI CCWWGG 1 cut(s) 1040
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 4 cut(s) 422, 523, 579, 667
TaqI TCGA 4 cut(s) 285, 294, 718, 960
TaqII GACCGA 1 cut(s) 980
TasI AATT 5 cut(s) 271, 446, 949, 1067, 1105
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 3 cut(s) 260, 358, 554
Tru1I TTAA 3 cut(s) 617, 693, 914
Tru9I TTAA 3 cut(s) 617, 693, 914
TscAI CASTG 2 cut(s) 311, 774
TseI GCWGC 1 cut(s) 836
TspDTI ATGAA 4 cut(s) 125, 209, 414, 546
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 2 cut(s) 311, 774
XapI RAATTY 2 cut(s) 446, 1067
XhoI CTCGAG 1 cut(s) 717
XspI CTAG 1 cut(s) 428
ZraI GACGTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.