AT3G17265

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000186
Physical Location & Seq
Reverse (-)
5899515 .. 5900637
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G17265.1

Sequence Viewer

Length: 1038 bp
ATGATGTTTGCTTATCTTCCACCTGATTTAGAATCGGAAATACTCTCTAGGGTTCCTGCCACATTTCTAAAAGAATTGCAAACTACTTGCAAACGATGGTACGCTTTATTTAGAGATCCGATATTCGTCAAGAAGAACTTGGGTAAAGCAGCAACGCATGTGATCTTTAATAATCTTGGGGATTATTCAGTGACAGAGATGAATACTTTGGTTCATAGCATCAATCTCCGTGGAATCCAAAACAGCTTTGATCCATCCATTGGTGTTGAACGTAAACTTTACGAGCTAAATGATCCTGAACATGATAAGATACTTGGTATTATCTCTCACTGCGATGGTTTATTATTGTGCGCTACCAAGGACAAGACTAGACTCGTGGTTTGGAACCCTTGTACTGGTCAAACCAGGTGGATCCAAATCAAGAATGTGTTCAAGAACAATCCCCAGCTTATTCTCTTGGTAATTAAGTTTAAAATCTATGAGTTCAACTCTGATTCATGGAGAATTCTTGATGACATCTCTCCTGTTTGTTTCATACGCTCAAATGGCGTGAATTTGAAGGGAAATGCTTATTTTGTTGCTTCAGATAAAGAAAGCAAATTCATACTCAAGTTTGATTTCACAACAGAGAGATTCCTACGTCTATCGTTTCCTTCTCAGAACGATGATCAAATTGCGGTTCTATCAGTTGTTAGACAAGAGAAACTTGCCTTGTTACAACAAAGGTTTGATACATCTTCAATAAAGATGAATATATGGGTGACCAAAACTAAGATCGATGCGGACAAAGACTTGTCTTGGAGCAACTTCTTAGTAGTAGATTTTGGTAAAGTTACATTAACAAGTGAGCCGTTGAGTTTCTTGGTGGACGAGGAGAATAAAATGGTCGTGTGTTCTTCAAAGATTCGTCACATTGAAAGGAAGACTATAATTTACTTTGCTAAAGAGGGTATACAAGTTCATCAAGAGATTGCACAGAAACCAAAAGGTTGTTGTTCATTTCTCGTCAGTTATGTTCCAAGTTTGGTTCAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210
Pfam Domains
Protein Families
PPR PPR_2 PPR_3 RRM_1

Protein Analysis

345

Amino Acids

39.72

Weight (kDa)

8.77

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 65 - 344 1.5e-69 F-box associated beta propeller domain
FBA_3 PF08268 156 - 313 4.2e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11608 AT1G11620 AT1G11810 AT1G12170 AT1G12190 AT1G24800 AT1G24881 AT1G25055 AT1G25141 AT1G25150 AT1G25150 AT1G25211 AT1G31510 AT1G32140 AT1G32430 AT1G47390 AT1G50470 AT1G51290 AT1G51320 AT1G53325 AT1G54550 AT1G58090 AT1G59630 AT1G59680 AT1G59680 AT1G62270 AT1G65990 AT1G66490 AT1G67450 AT1G67455 AT1G67455 AT1G77650 AT1G77655 AT2G04920 AT2G07120 AT2G07140 AT2G07140 AT2G11200 AT2G14710 AT2G17310 AT2G17830 AT2G18780 AT2G24510 AT2G27520 AT2G33655 AT2G38590 AT2G39355 AT3G03405 AT3G04250 AT3G08750 AT3G10430 AT3G13680 AT3G13820 AT3G13830 AT3G14030 AT3G16020 AT3G16020 AT3G16020 AT3G16555 AT3G16580 AT3G16590 AT3G16740 AT3G16820 AT3G16880 AT3G17265 AT3G17270 AT3G17280 AT3G17280 AT3G17320 AT3G17400 AT3G17480 AT3G17490 AT3G17500 AT3G17500 AT3G17530 AT3G17540 AT3G17560 AT3G17570 AT3G17620 AT3G17710 AT3G18120 AT3G18320 AT3G18330 AT3G18340 AT3G18905 AT3G18910 AT3G18980 AT3G18980 AT3G19410 AT3G19470 AT3G19470 AT3G19560 AT3G19565 AT3G19880 AT3G19890 AT3G20030 AT3G20400 AT3G20690 AT3G20700 AT3G20710 AT3G21120 AT3G21130 AT3G21170 AT3G21410 AT3G22350 AT3G22350 AT3G22421 AT3G22650 AT3G22650 AT3G22700 AT3G22710 AT3G22720 AT3G22730 AT3G22770 AT3G22870 AT3G22940 AT3G23260 AT3G23420 AT3G23420 AT3G23633 AT3G23633 AT3G23680 AT3G23685 AT3G24580 AT3G24700 AT3G24700 AT3G25090 AT3G25090 AT3G25460 AT3G44120 AT3G44130 AT3G49510 AT3G49520 AT3G49980 AT3G49980 AT3G51171 AT3G59590 AT3G59610 AT4G04690 AT4G05080 AT4G09870 AT4G10190 AT4G10740 AT4G17200 AT4G33290 AT5G25195 AT5G36190 AT5G36200 AT5G36730 AT5G36820 AT5G37040 AT5G41490 AT5G41500 AT5G41510 AT5G42460 AT5G47300 AT5G51000 AT5G60560 AT5G60560 AT5G62830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 260
AccI GTMKAC 1 cut(s) 952
AciI CCGC 2 cut(s) 677, 782
AclWI GGATC 5 cut(s) 110, 245, 287, 406, 419
AcsI RAATTY 3 cut(s) 504, 553, 599
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 101, 394
AfiI CCNNNNNNNGG 2 cut(s) 260, 395
AgsI TTSAA 8 cut(s) 269, 433, 487, 559, 741, 900, 917, 1031
AjnI CCWGG 1 cut(s) 404
AluBI AGCT 3 cut(s) 246, 286, 448
AluI AGCT 3 cut(s) 246, 286, 448
AlwI GGATC 5 cut(s) 110, 245, 287, 406, 419
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 3 cut(s) 504, 553, 599
ArsI GACNNNNNNTTYG 2 cut(s) 363, 395
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 772
BamHI GGATCC 1 cut(s) 411
BauI CACGAG 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 929
BbvI GCAGC 1 cut(s) 161
BccI CCATC 3 cut(s) 90, 262, 329
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 1 cut(s) 406
BclI TGATCA 1 cut(s) 667
BfaI CTAG 2 cut(s) 48, 369
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 406
BmiI GGNNCC 3 cut(s) 54, 386, 413
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 228, 769
BpiI GAAGAC 1 cut(s) 929
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpuEI CTTGAG 1 cut(s) 593
Bsa29I ATCGAT 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 229, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 395
Bse1I ACTGG 1 cut(s) 400
Bse8I GATNNNNATC 1 cut(s) 416
BseBI CCWGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 229, 357
BseGI GGATG 1 cut(s) 254
BseJI GATNNNNATC 1 cut(s) 416
BseLI CCNNNNNNNGG 2 cut(s) 260, 395
BseMII CTCAG 1 cut(s) 671
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 887
BseXI GCAGC 1 cut(s) 161
BseYI CCCAGC 1 cut(s) 444
BshVI ATCGAT 1 cut(s) 777
BslI CCNNNNNNNGG 2 cut(s) 260, 395
Bsp143I GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
BspACI CCGC 2 cut(s) 677, 782
BspCNI CTCAG 1 cut(s) 670
BspDI ATCGAT 1 cut(s) 777
BspLI GGNNCC 3 cut(s) 54, 386, 413
BspPI GGATC 5 cut(s) 110, 245, 287, 406, 419
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 229, 357
BssMI GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
BssNAI GTATAC 1 cut(s) 953
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 357
Bst1107I GTATAC 1 cut(s) 953
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 1 cut(s) 406
BstDEI CTNAG 3 cut(s) 657, 771, 811
BstDSI CCRYGG 1 cut(s) 229
BstEII GGTNACC 1 cut(s) 760
BstF5I GGATG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 7 cut(s) 118, 165, 253, 295, 414, 670, 777
BstMBI GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
BstMWI GCNNNNNNNGC 1 cut(s) 546
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 1 cut(s) 161
BstPI GGTNACC 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 929
BstX2I RGATCY 2 cut(s) 115, 411
BstYI RGATCY 2 cut(s) 115, 411
BstZ17I GTATAC 1 cut(s) 953
Bsu15I ATCGAT 1 cut(s) 777
BsuTUI ATCGAT 1 cut(s) 777
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 254
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 195, 328
CfoI GCGC 1 cut(s) 353
ClaI ATCGAT 1 cut(s) 777
CsiI ACCWGGT 1 cut(s) 404
Csp6I GTAC 2 cut(s) 100, 393
CspCI CAANNNNNGTGG 4 cut(s) 211, 246, 389, 424
CviAII CATG 3 cut(s) 158, 302, 498
CviJI RGCY 4 cut(s) 246, 286, 448, 850
CviKI_1 RGCY 4 cut(s) 246, 286, 448, 850
CviQI GTAC 2 cut(s) 100, 393
DdeI CTNAG 3 cut(s) 657, 771, 811
DpnI GATC 7 cut(s) 117, 164, 252, 294, 413, 669, 776
DpnII GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
DraI TTTAAA 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 567
Eco91I GGTNACC 1 cut(s) 760
EcoO65I GGTNACC 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 3 cut(s) 161, 305, 501
FalI AAGNNNNNCTT 4 cut(s) 122, 154, 690, 722
FatI CATG 3 cut(s) 157, 301, 497
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 952
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 2 cut(s) 48, 369
GlaI GCGC 1 cut(s) 352
GluI GCNGC 1 cut(s) 150
GsaI CCCAGC 1 cut(s) 448
HhaI GCGC 1 cut(s) 353
Hin1II CATG 3 cut(s) 161, 305, 501
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 6 cut(s) 32, 234, 372, 494, 633, 904
HphI GGTGA 1 cut(s) 772
Hpy166II GTNNAC 3 cut(s) 275, 868, 953
Hpy188I TCNGA 5 cut(s) 37, 120, 493, 586, 660
Hpy188III TCNNGA 6 cut(s) 130, 296, 421, 433, 509, 965
Hpy8I GTNNAC 3 cut(s) 275, 868, 953
HpyAV CCTTC 2 cut(s) 553, 663
HpyCH4IV ACGT 2 cut(s) 271, 640
HpyCH4V TGCA 3 cut(s) 79, 90, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 546
HpyF3I CTNAG 3 cut(s) 657, 771, 811
HpySE526I ACGT 2 cut(s) 271, 640
Hsp92II CATG 3 cut(s) 161, 305, 501
HspAI GCGC 1 cut(s) 351
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
LmnI GCTCC 1 cut(s) 801
LpnPI CCDG 8 cut(s) 36, 69, 309, 381, 391, 418, 458, 537
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 2 cut(s) 228, 769
MabI ACCWGGT 1 cut(s) 404
MaeI CTAG 2 cut(s) 48, 369
MaeII ACGT 2 cut(s) 271, 640
MaeIII GTNAC 5 cut(s) 190, 714, 760, 832, 908
MalI GATC 7 cut(s) 117, 164, 252, 294, 413, 669, 776
MboI GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
MboII GAAGA 5 cut(s) 8, 145, 729, 888, 934
MflI RGATCY 2 cut(s) 115, 411
MluCI AATT 8 cut(s) 74, 462, 504, 553, 599, 672, 930, 1031
MlyI GAGTC 1 cut(s) 366
MnlI CCTC 2 cut(s) 865, 940
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 4 cut(s) 168, 465, 471, 839
MslI CAYNNNNRTG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 406
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 546
NdeII GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
NlaIII CATG 3 cut(s) 161, 305, 501
NlaIV GGNNCC 3 cut(s) 54, 386, 413
NmuCI GTSAC 3 cut(s) 190, 760, 908
NspI RCATGY 1 cut(s) 161
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 5 cut(s) 32, 234, 494, 633, 904
PflMI CCANNNNNTGG 1 cut(s) 260
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 404
PspEI GGTNACC 1 cut(s) 760
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 3 cut(s) 54, 386, 413
PsuI RGATCY 2 cut(s) 115, 411
RsaI GTAC 2 cut(s) 101, 394
RsaNI GTAC 2 cut(s) 100, 393
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 4 cut(s) 168, 465, 471, 839
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 7 cut(s) 115, 162, 250, 292, 411, 667, 774
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 1 cut(s) 406
SetI ASST 9 cut(s) 25, 248, 274, 288, 410, 450, 643, 728, 991
SexAI ACCWGGT 1 cut(s) 404
SfaNI GCATC 2 cut(s) 228, 769
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 8 cut(s) 74, 462, 504, 553, 599, 672, 930, 1031
SsiI CCGC 2 cut(s) 677, 782
SspMI CTAG 2 cut(s) 48, 369
StyD4I CCNGG 1 cut(s) 404
StyI CCWWGG 1 cut(s) 357
TaiI ACGT 2 cut(s) 274, 643
TaqI TCGA 1 cut(s) 777
TasI AATT 8 cut(s) 74, 462, 504, 553, 599, 672, 930, 1031
TatI WGTACW 1 cut(s) 392
TfiI GAWTC 5 cut(s) 32, 234, 494, 633, 904
Tru1I TTAA 4 cut(s) 168, 465, 471, 839
Tru9I TTAA 4 cut(s) 168, 465, 471, 839
TscAI CASTG 2 cut(s) 195, 335
TseFI GTSAC 3 cut(s) 190, 760, 908
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 3 cut(s) 190, 760, 908
TspDTI ATGAA 8 cut(s) 203, 215, 486, 523, 592, 764, 950, 987
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 195, 335
Van91I CCANNNNNTGG 1 cut(s) 260
XapI RAATTY 3 cut(s) 504, 553, 599
XceI RCATGY 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 952
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 2 cut(s) 48, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.