pycom13g16660

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000063
Physical Location & Seq
Forward (+)
12424216 .. 12425559
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16660.1

Sequence Viewer

Length: 1344 bp
ATGAGCTCCTTCTTCTCCAAGGAGTGGAAGGCTCTCGGCATCTACGATGCCATTATTCTGTCAACCATGGAGATTGCCATGGATAAGGAGCTGCTCATGGCGGCCCTGAGTTTATGGTGTTCGGCCACCAACACCATGGTCCTGCCTTTCGGCCCTCTCGGCCCCACCATCCTCGACGTCTCGGCCATCCTTGGGACTTCCTCGACCGGCCTTCCTATTGACGCCGCCCTTTCCGGATACCCTTCTCCTCTCGACCTGAAGGCACTATTCGACGAAAGGGCCGTCGAGACGCTAAGCCGGGGTGATCGGGAGCCCTCAAGGGAAGAAGTTCAGAAGCTCCACAAGAACTTCTTCAACTACAACACTCTCATTCTTCATTTCGCCGGCCGAGGCGAGGAAAGCCTTAGGAAGGGAGAGCACGAGGCCTTCCTCTTCTACTGGTACAACAAATTCATTTGTTGTACTCGGTCGAACAAATGCCTGGTGGAGAACATGCCGGTGGCCCAGGCACTGGCTAATGGTCGCTTGCTGGCGCTCAGTCCAGCCATACTCGCCAATCTCTACCGTTGCCTGGCCGAGGCGACCATTGGCAAGATCGACCCGCATCAGAACGGACCCCTCTGGGTGTTCCAACTCTGGTTGCAGGTTTACTTCTCTACTCTTCGACCGGAGGTCCCTGCCTTCCAGCGAACGGCCGCCCTCGGTCTTCAACTGGCATCTCGACCGGCACCACCTCACCGAGCCGACGAGGTTTTCAAACACTTCCTCGGTCTAGATGTCCTCTCGGATGACGAGTTCCTGGTGTGCCGGCGCCAGGAGTACCCTTCCTCGCTCAAGCTTCCTACGGCCGCGTGGGCCGCGTCCGAGGATGCGGACATTCATCAGCGCTGGGGGTCGTTTGTTTTGGCTCGCGACCTTCCACTGGGTTGCGATGCCCGCCGAGCTGGCTGGGAGGTCTACCACCCCCATTTTGTCGCCCGGCAGCTCGGCTATATTCAAGGCAGCCCCGTCCCCCTGCTCGCTTCTCGGTCACTCCTAAGTCGGGGACGTCTTACCGGTTCCTCTGAGAGGGAGTGCAAGACGTCCGACCAGGAGTTTAAAGAGAAGTGCAGGAAATTCCGGATTCGGCCGGCGGTCTCTGAATCTCTGGGAACTGATACCTTCGGGGACTGGTGGGAGGAGTATACCGGCAACTTCTTCGGCGCCCCGGCCGAAGATCTGGCGAAGAAGATCTTCGGCGACCGCCCTAGGTCAATATCCTCGACACAGCCTAAGGAGTCGACCCAAGGTAGTCTTTCCGTTTTTCTTTCCTCCTTTGTTTTACCACTTGATCCTCGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase cNMP_binding

Protein Analysis

448

Amino Acids

49.99

Weight (kDa)

6.53

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 13 - 393 2.5e-38 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000063)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21382 FvH4_1g29794 FvH4_1g29795 FvH4_2g35573 FvH4_2g35574 FvH4_4g18552 FvH4_5g16044
malus_domestica MD05G1017000.v1.1
prunus_persica Prupe.3G140800_v2.0.a1 Prupe.6G164100_v2.0.a1
pyrus_communis pycom01g04330 pycom03g19820 pycom04g06720 pycom06g11360 pycom06g19000 pycom10g08100 pycom13g16660 pycom13g27590 pycom13g27640 pycom13g27890 pycom16g11770 pycom16g19530
rosa_chinensis RchiOBHm_Chr1g0317341 RchiOBHm_Chr1g0317351 RchiOBHm_Chr1g0320431 RchiOBHm_Chr1g0320711 RchiOBHm_Chr1g0320721 RchiOBHm_Chr1g0321611 RchiOBHm_Chr1g0321621 RchiOBHm_Chr1g0321631 RchiOBHm_Chr1g0323651 RchiOBHm_Chr1g0332991 RchiOBHm_Chr1g0333001 RchiOBHm_Chr1g0335981 RchiOBHm_Chr1g0335991 RchiOBHm_Chr1g0336001 RchiOBHm_Chr1g0350201 RchiOBHm_Chr1g0368201 RchiOBHm_Chr2g0105531 RchiOBHm_Chr2g0105541 RchiOBHm_Chr2g0116571 RchiOBHm_Chr2g0116581 RchiOBHm_Chr2g0121071 RchiOBHm_Chr2g0131351 RchiOBHm_Chr2g0131661 RchiOBHm_Chr2g0133021 RchiOBHm_Chr2g0138181 RchiOBHm_Chr2g0138191 RchiOBHm_Chr2g0142701 RchiOBHm_Chr2g0142711 RchiOBHm_Chr2g0152981 RchiOBHm_Chr3g0456181 RchiOBHm_Chr3g0456191 RchiOBHm_Chr3g0493631 RchiOBHm_Chr3g0496201 RchiOBHm_Chr4g0401541 RchiOBHm_Chr4g0410311 RchiOBHm_Chr4g0410321 RchiOBHm_Chr4g0419231 RchiOBHm_Chr4g0419511 RchiOBHm_Chr4g0419521 RchiOBHm_Chr5g0004311 RchiOBHm_Chr5g0004321 RchiOBHm_Chr5g0038131 RchiOBHm_Chr5g0038251 RchiOBHm_Chr5g0038261 RchiOBHm_Chr5g0038271 RchiOBHm_Chr5g0052241 RchiOBHm_Chr5g0053911 RchiOBHm_Chr5g0074641 RchiOBHm_Chr5g0076651 RchiOBHm_Chr5g0076661 RchiOBHm_Chr6g0249161 RchiOBHm_Chr6g0249171 RchiOBHm_Chr6g0250961 RchiOBHm_Chr6g0253701 RchiOBHm_Chr6g0262131 RchiOBHm_Chr6g0263181 RchiOBHm_Chr6g0263231 RchiOBHm_Chr6g0263241 RchiOBHm_Chr6g0263251 RchiOBHm_Chr6g0291271 RchiOBHm_Chr7g0194611 RchiOBHm_Chr7g0219691 RchiOBHm_Chr7g0219701 RchiOBHm_Chr7g0225431 RchiOBHm_Chr7g0234221 RchiOBHm_Chr7g0238711 RchiOBHm_Chr7g0238721 RchiOBHm_Chr7g0238731 RchiOBHm_Chr7g0239611 RchiOBHm_Chr7g0239621
rosa_laevigata RLG00000001361 RLG00000001376 RLG00000001860 RLG00000002051 RLG00000002362 RLG00000008940 RLG00000012764 RLG00000018782 RLG00000029639 RLG00000030151
rosa_multiflora Rmu_co8090794.1_g000001 Rmu_co8135356.1_g000001 Rmu_co8224968.1_g000001 Rmu_sc0000016.1_g000020 Rmu_sc0000029.1_g000061 Rmu_sc0000035.1_g000032 Rmu_sc0000060.1_g000041 Rmu_sc0000148.1_g000041 Rmu_sc0000315.1_g000084 Rmu_sc0000527.1_g000005 Rmu_sc0000527.1_g000007 Rmu_sc0000527.1_g000008 Rmu_sc0000648.1_g000002 Rmu_sc0000762.1_g000076 Rmu_sc0000870.1_g000003 Rmu_sc0000870.1_g000004 Rmu_sc0000948.1_g000037 Rmu_sc0000997.1_g000003 Rmu_sc0001047.1_g000041 Rmu_sc0001047.1_g000042 Rmu_sc0001075.1_g000044 Rmu_sc0001174.1_g000006 Rmu_sc0001235.1_g000025 Rmu_sc0001292.1_g000008 Rmu_sc0001301.1_g000038 Rmu_sc0001461.1_g000069 Rmu_sc0001461.1_g000070 Rmu_sc0001669.1_g000041 Rmu_sc0001689.1_g000027 Rmu_sc0001896.1_g000008 Rmu_sc0001909.1_g000013 Rmu_sc0002170.1_g000038 Rmu_sc0002200.1_g000014 Rmu_sc0002283.1_g000027 Rmu_sc0002284.1_g000014 Rmu_sc0002294.1_g000017 Rmu_sc0002294.1_g000018 Rmu_sc0002307.1_g000020 Rmu_sc0002307.1_g000021 Rmu_sc0002307.1_g000023 Rmu_sc0002357.1_g000054 Rmu_sc0002409.1_g000005 Rmu_sc0002535.1_g000002 Rmu_sc0002589.1_g000014 Rmu_sc0002759.1_g000016 Rmu_sc0002759.1_g000017 Rmu_sc0002777.1_g000015 Rmu_sc0002824.1_g000016 Rmu_sc0002872.1_g000005 Rmu_sc0002872.1_g000012 Rmu_sc0002872.1_g000013 Rmu_sc0002942.1_g000019 Rmu_sc0003337.1_g000001 Rmu_sc0003410.1_g000052 Rmu_sc0003445.1_g000010 Rmu_sc0003773.1_g000007 Rmu_sc0003773.1_g000008 Rmu_sc0003895.1_g000002 Rmu_sc0004063.1_g000006 Rmu_sc0004088.1_g000009 Rmu_sc0004088.1_g000010 Rmu_sc0004174.1_g000027 Rmu_sc0004196.1_g000010 Rmu_sc0004515.1_g000006 Rmu_sc0004727.1_g000010 Rmu_sc0004755.1_g000018 Rmu_sc0005016.1_g000030 Rmu_sc0005026.1_g000001 Rmu_sc0005044.1_g000007 Rmu_sc0005284.1_g000001 Rmu_sc0005431.1_g000030 Rmu_sc0005802.1_g000014 Rmu_sc0005802.1_g000015 Rmu_sc0005994.1_g000004 Rmu_sc0005994.1_g000005 Rmu_sc0006059.1_g000048 Rmu_sc0006583.1_g000013 Rmu_sc0006824.1_g000009 Rmu_sc0007458.1_g000005 Rmu_sc0007707.1_g000002 Rmu_sc0008242.1_g000012 Rmu_sc0008713.1_g000006 Rmu_sc0008770.1_g000008 Rmu_sc0009785.1_g000004 Rmu_sc0009785.1_g000005 Rmu_sc0010125.1_g000001 Rmu_sc0010211.1_g000004 Rmu_sc0011034.1_g000001 Rmu_sc0011833.1_g000006 Rmu_sc0014851.1_g000001 Rmu_sc0014899.1_g000001 Rmu_sc0015060.1_g000003 Rmu_sc0015186.1_g000001 Rmu_sc0015248.1_g000003 Rmu_sc0015248.1_g000004 Rmu_sc0015314.1_g000001 Rmu_sc0018098.1_g000002 Rmu_sc0022706.1_g000004 Rmu_sc0023350.1_g000002 Rmu_sc0027636.1_g000001 Rmu_sc0027672.1_g000003 Rmu_sc0030879.1_g000001 Rmu_ssc0000008.1_g000029 Rmu_ssc0000019.1_g000014 Rmu_ssc0000050.1_g000082 Rmu_ssc0000282.1_g000004 Rmu_ssc0000311.1_g000010
rosa_roxburghii Rroxscaffold_1G00015190 Rroxscaffold_1G00031880 Rroxscaffold_1G00031890 Rroxscaffold_1G00059310 Rroxscaffold_1G00060420 Rroxscaffold_1G00067930 Rroxscaffold_1G00075030 Rroxscaffold_2G00082210 Rroxscaffold_2G00122970 Rroxscaffold_3G00218230 Rroxscaffold_3G00219020 Rroxscaffold_3G00219030 Rroxscaffold_3G00223680 Rroxscaffold_3G00223690 Rroxscaffold_3G00223700 Rroxscaffold_3G00226870 Rroxscaffold_3G00226880 Rroxscaffold_3G00232990 Rroxscaffold_3G00265610 Rroxscaffold_3G00265620 Rroxscaffold_3G00265630 Rroxscaffold_4G00318660 Rroxscaffold_4G00319060 Rroxscaffold_4G00323500 Rroxscaffold_5G00357520 Rroxscaffold_5G00366150 Rroxscaffold_5G00377470 Rroxscaffold_6G00395700 Rroxscaffold_6G00413950 Rroxscaffold_7G00187650 Rroxscaffold_7G00198290 Rroxscaffold_7G00198300 Rroxscaffold_7G00208060
rosa_rugosa Rorug01G0173900 Rorug01G0174000 Rorug01G0174100 Rorug01G0174100 Rorug02G0341200 Rorug04G0135700 Rorug04G0135800 Rorug05G0032500 Rorug06G0021800
rosa_samantha Rh1AG036600 Rh1AG245800 Rh1CG021700 Rh1CG021800 Rh1CG085800 Rh1CG085900 Rh1CG187900 Rh1CG229600 Rh1CG239600 Rh1CG246500 Rh1CG246600 Rh2BG478100 Rh2DG341300 Rh2DG366600 Rh2DG375700 Rh2DG384300 Rh2DG487000 Rh2DG504000 Rh2DG504100 Rh3AG311800 Rh3AG327500 Rh3AG328300 Rh3BG345100 Rh3CG320100 Rh4AG005700 Rh4AG067900 Rh4AG068000 Rh4AG084400 Rh4AG106700 Rh4AG160000 Rh4AG173900 Rh4AG174000 Rh4CG125300 Rh4CG125500 Rh4CG264500 Rh4DG110800 Rh5AG047300 Rh5AG085200 Rh5AG126300 Rh5AG127300 Rh5AG203000 Rh5AG332400 Rh5AG332500 Rh5AG342400 Rh5AG344100 Rh5AG375600 Rh5AG378300 Rh5AG386000 Rh5AG529600 Rh5BG125200 Rh5CG137600 Rh5CG137700 Rh5DG125200 Rh6BG134100 Rh6BG175400 Rh6BG179500 Rh6BG179600 Rh6BG181600 Rh6BG181700 Rh6BG191100 Rh6BG232900 Rh6BG259700 Rh6BG259800 Rh7AG028000 Rh7AG319400 Rh7AG352700 Rh7AG352800 Rh7AG352900 Rh7AG482500 Rh7AG482600 Rh7AG503800 Rh7BG334700 Rh7CG200100 Rh7CG336200 Rh7CG370200 Rh7CG370400 Rh7CG370500
rosa_wichuraiana Rw1G001580 Rw1G003570 Rw1G004260 Rw1G004270 Rw1G021170 Rw2G019390 Rw2G040280 Rw2G044050 Rw4G004430 Rw5G010950 Rw5G018350 Rw5G032890 Rw6G002840 Rw6G006510 Rw6G007780 Rw6G033790 Rw7G036320 Rw7G040550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 180, 1051, 1085
Acc36I ACCTGC 1 cut(s) 634
AccB1I GGYRCC 3 cut(s) 727, 810, 1202
AccB7I CCANNNNNTGG 2 cut(s) 24, 511
AccI GTMKAC 3 cut(s) 957, 1184, 1280
AccII CGCG 3 cut(s) 851, 860, 912
AccIII TCCGGA 2 cut(s) 233, 1119
AclWI GGATC 1 cut(s) 1325
AcoI YGGCCR 8 cut(s) 123, 183, 385, 573, 693, 846, 1127, 1209
AcsI RAATTY 2 cut(s) 449, 1115
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
AfaI GTAC 3 cut(s) 443, 463, 821
AfeI AGCGCT 1 cut(s) 887
AgeI ACCGGT 1 cut(s) 1055
AgsI TTSAA 4 cut(s) 355, 710, 757, 998
AhdI GACNNNNNGTC 1 cut(s) 671
AjnI CCWGG 6 cut(s) 480, 504, 570, 798, 813, 1089
AloI GAACNNNNNNTCC 2 cut(s) 779, 811
AluBI AGCT 6 cut(s) 6, 91, 337, 838, 944, 985
AluI AGCT 6 cut(s) 6, 91, 337, 838, 944, 985
Alw21I GWGCWC 2 cut(s) 8, 420
Alw26I GTCTC 3 cut(s) 184, 281, 1141
AlwI GGATC 1 cut(s) 1325
AlwNI CAGNNNCTG 1 cut(s) 511
Aor13HI TCCGGA 2 cut(s) 233, 1119
Aor51HI AGCGCT 1 cut(s) 887
ApeKI GCWGC 3 cut(s) 91, 982, 1002
ApoI RAATTY 2 cut(s) 449, 1115
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AsiGI ACCGGT 1 cut(s) 1055
Asp700I GAANNNNTTC 3 cut(s) 327, 350, 1232
AspA2I CCTAGG 1 cut(s) 1247
AspLEI GCGC 4 cut(s) 535, 813, 888, 1205
AspS9I GGNCC 9 cut(s) 103, 139, 152, 161, 279, 502, 614, 673, 855
AsuC2I CCSGG 3 cut(s) 299, 979, 1208
AsuHPI GGTGA 2 cut(s) 314, 728
AvaII GGWCC 3 cut(s) 139, 614, 673
AvrII CCTAGG 1 cut(s) 1247
AxyI CCTNAGG 2 cut(s) 404, 1272
BanI GGYRCC 3 cut(s) 727, 810, 1202
BanII GRGCYC 2 cut(s) 8, 315
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 2 cut(s) 8, 420
BbvI GCAGC 3 cut(s) 78, 994, 1014
BccI CCATC 2 cut(s) 176, 194
BceAI ACGGC 3 cut(s) 266, 708, 861
BcgI CGANNNNNNTGC 2 cut(s) 1180, 1214
BciT130I CCWGG 6 cut(s) 482, 506, 572, 800, 815, 1091
BciVI GTATCC 1 cut(s) 230
BcnI CCSGG 3 cut(s) 299, 979, 1208
BcoDI GTCTC 3 cut(s) 184, 281, 1141
BfaI CTAG 2 cut(s) 773, 1248
BfoI RGCGCY 4 cut(s) 536, 814, 889, 1206
BfuAI ACCTGC 1 cut(s) 634
BfuI GTATCC 1 cut(s) 230
BglI GCCNNNNNGGC 3 cut(s) 159, 854, 945
BglII AGATCT 2 cut(s) 1216, 1230
BisI GCNGC 8 cut(s) 92, 102, 225, 696, 849, 858, 983, 1003
BlnI CCTAGG 1 cut(s) 1247
BlpI GCTNAGC 1 cut(s) 293
BlsI GCNGC 8 cut(s) 93, 103, 226, 697, 850, 859, 984, 1004
Bme1390I CCNGG 9 cut(s) 299, 482, 506, 572, 800, 815, 979, 1091, 1208
Bme18I GGWCC 3 cut(s) 139, 614, 673
BmeRI GACNNNNNGTC 1 cut(s) 671
BmgT120I GGNCC 9 cut(s) 103, 139, 152, 161, 279, 502, 614, 673, 855
BmiI GGNNCC 8 cut(s) 163, 312, 616, 675, 729, 812, 1060, 1204
BmrFI CCNGG 9 cut(s) 299, 482, 506, 572, 800, 815, 979, 1091, 1208
BmrI ACTGGG 1 cut(s) 932
BmsI GCATC 6 cut(s) 37, 48, 613, 725, 859, 922
BmuI ACTGGG 1 cut(s) 932
BpiI GAAGAC 1 cut(s) 698
Bpu1102I GCTNAGC 1 cut(s) 293
BpuEI CTTGAG 2 cut(s) 301, 818
BpuMI CCSGG 3 cut(s) 299, 979, 1208
BsaHI GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
BsaI GGTCTC 1 cut(s) 1141
BsaWI WCCGGW 4 cut(s) 233, 667, 1055, 1119
Bse118I RCCGGY 8 cut(s) 206, 383, 496, 724, 807, 1055, 1129, 1187
Bse1I ACTGG 5 cut(s) 443, 516, 717, 927, 1175
Bse21I CCTNAGG 2 cut(s) 404, 1272
BseAI TCCGGA 2 cut(s) 233, 1119
BseBI CCWGG 6 cut(s) 482, 506, 572, 800, 815, 1091
BseGI GGATG 4 cut(s) 168, 186, 793, 874
BseMII CTCAG 3 cut(s) 98, 550, 1056
BseNI ACTGG 5 cut(s) 443, 516, 717, 927, 1175
BseRI GAGGAG 2 cut(s) 237, 1193
BseX3I CGGCCG 5 cut(s) 385, 693, 846, 1127, 1209
BseXI GCAGC 3 cut(s) 78, 994, 1014
BseYI CCCAGC 2 cut(s) 888, 948
BsgI GTGCAG 1 cut(s) 1129
Bsh1236I CGCG 3 cut(s) 851, 860, 912
BshNI GGYRCC 3 cut(s) 727, 810, 1202
BshTI ACCGGT 1 cut(s) 1055
BsiHKAI GWGCWC 2 cut(s) 8, 420
BslFI GGGAC 5 cut(s) 208, 659, 995, 1059, 1181
BsmAI GTCTC 3 cut(s) 184, 281, 1141
BsmBI CGTCTC 2 cut(s) 184, 281
BsmFI GGGAC 5 cut(s) 208, 659, 995, 1059, 1181
Bso31I GGTCTC 1 cut(s) 1141
Bsp1286I GDGCHC 3 cut(s) 8, 315, 420
Bsp13I TCCGGA 2 cut(s) 233, 1119
Bsp143I GATC 5 cut(s) 304, 594, 1216, 1230, 1330
Bsp1720I GCTNAGC 1 cut(s) 293
Bsp19I CCATGG 3 cut(s) 66, 78, 135
Bsp68I TCGCGA 1 cut(s) 912
BspCNI CTCAG 3 cut(s) 99, 549, 1057
BspEI TCCGGA 2 cut(s) 233, 1119
BspFNI CGCG 3 cut(s) 851, 860, 912
BspLI GGNNCC 8 cut(s) 163, 312, 616, 675, 729, 812, 1060, 1204
BspMI ACCTGC 1 cut(s) 634
BspPI GGATC 1 cut(s) 1325
BspT107I GGYRCC 3 cut(s) 727, 810, 1202
BspTNI GGTCTC 1 cut(s) 1141
BsrFI RCCGGY 8 cut(s) 206, 383, 496, 724, 807, 1055, 1129, 1187
BsrI ACTGG 5 cut(s) 443, 516, 717, 927, 1175
BssAI RCCGGY 8 cut(s) 206, 383, 496, 724, 807, 1055, 1129, 1187
BssMI GATC 5 cut(s) 304, 594, 1216, 1230, 1330
BssNAI GTATAC 1 cut(s) 1185
BssNI GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 7 cut(s) 18, 66, 78, 135, 190, 1247, 1285
Bst1107I GTATAC 1 cut(s) 1185
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 6 cut(s) 482, 506, 572, 800, 815, 1091
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 2 cut(s) 437, 666
BstACI GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
BstC8I GCNNGC 9 cut(s) 385, 527, 531, 809, 910, 937, 946, 1020, 1131
BstDEI CTNAG 7 cut(s) 107, 293, 404, 536, 1037, 1065, 1272
BstDSI CCRYGG 3 cut(s) 66, 78, 135
BstENI CCTNNNNNAGG 3 cut(s) 407, 575, 1066
BstF5I GGATG 4 cut(s) 168, 186, 793, 874
BstFNI CGCG 3 cut(s) 851, 860, 912
BstH2I RGCGCY 4 cut(s) 536, 814, 889, 1206
BstHHI GCGC 4 cut(s) 535, 813, 888, 1205
BstKTI GATC 5 cut(s) 307, 597, 1219, 1233, 1333
BstMAI GTCTC 3 cut(s) 184, 281, 1141
BstMBI GATC 5 cut(s) 304, 594, 1216, 1230, 1330
BstMWI GCNNNNNNNGC 8 cut(s) 159, 399, 551, 854, 857, 936, 941, 945
BstNI CCWGG 6 cut(s) 482, 506, 572, 800, 815, 1091
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 9 cut(s) 297, 480, 504, 570, 798, 813, 977, 1089, 1206
BstUI CGCG 3 cut(s) 851, 860, 912
BstV1I GCAGC 3 cut(s) 78, 994, 1014
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 2 cut(s) 1216, 1230
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 2 cut(s) 1216, 1230
BstZ17I GTATAC 1 cut(s) 1185
BstZI CGGCCG 5 cut(s) 385, 693, 846, 1127, 1209
Bsu36I CCTNAGG 2 cut(s) 404, 1272
BsuI GTATCC 1 cut(s) 230
BtgI CCRYGG 3 cut(s) 66, 78, 135
BtgZI GCGATG 1 cut(s) 945
BtsCI GGATG 4 cut(s) 168, 186, 793, 874
BtsIMutI CAGTG 2 cut(s) 509, 920
BtuMI TCGCGA 1 cut(s) 912
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 9 cut(s) 385, 527, 531, 809, 910, 937, 946, 1020, 1131
CaiI CAGNNNCTG 1 cut(s) 511
CfoI GCGC 4 cut(s) 535, 813, 888, 1205
Cfr10I RCCGGY 8 cut(s) 206, 383, 496, 724, 807, 1055, 1129, 1187
Cfr13I GGNCC 9 cut(s) 103, 139, 152, 161, 279, 502, 614, 673, 855
CseI GACGC 3 cut(s) 230, 298, 849
Csp6I GTAC 3 cut(s) 442, 462, 820
CspAI ACCGGT 1 cut(s) 1055
CviAII CATG 5 cut(s) 67, 79, 97, 136, 493
CviQI GTAC 3 cut(s) 442, 462, 820
DdeI CTNAG 7 cut(s) 107, 293, 404, 536, 1037, 1065, 1272
DinI GGCGCC 2 cut(s) 812, 1204
DpnI GATC 5 cut(s) 306, 596, 1218, 1232, 1332
DpnII GATC 5 cut(s) 304, 594, 1216, 1230, 1330
DraI TTTAAA 1 cut(s) 1099
DriI GACNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 8 cut(s) 123, 183, 385, 573, 693, 846, 1127, 1209
EagI CGGCCG 5 cut(s) 385, 693, 846, 1127, 1209
Eam1104I CTCTTC 2 cut(s) 437, 666
Eam1105I GACNNNNNGTC 1 cut(s) 671
EarI CTCTTC 2 cut(s) 437, 666
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 5 cut(s) 385, 693, 846, 1127, 1209
Eco130I CCWWGG 7 cut(s) 18, 66, 78, 135, 190, 1247, 1285
Eco147I AGGCCT 1 cut(s) 425
Eco24I GRGCYC 2 cut(s) 8, 315
Eco31I GGTCTC 1 cut(s) 1141
Eco47I GGWCC 3 cut(s) 139, 614, 673
Eco47III AGCGCT 1 cut(s) 887
Eco52I CGGCCG 5 cut(s) 385, 693, 846, 1127, 1209
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 278
Eco81I CCTNAGG 2 cut(s) 404, 1272
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 3 cut(s) 407, 575, 1066
EcoO109I RGGNCCY 1 cut(s) 673
EcoRII CCWGG 6 cut(s) 480, 504, 570, 798, 813, 1089
EcoT14I CCWWGG 7 cut(s) 18, 66, 78, 135, 190, 1247, 1285
EcoT38I GRGCYC 2 cut(s) 8, 315
EgeI GGCGCC 2 cut(s) 812, 1204
EheI GGCGCC 2 cut(s) 812, 1204
ErhI CCWWGG 7 cut(s) 18, 66, 78, 135, 190, 1247, 1285
Esp3I CGTCTC 2 cut(s) 184, 281
FaeI CATG 5 cut(s) 70, 82, 100, 139, 496
FaiI YATR 9 cut(s) 68, 80, 98, 115, 137, 494, 548, 993, 1185
FalI AAGNNNNNCTT 6 cut(s) 335, 367, 1217, 1249, 1278, 1310
FaqI GGGAC 5 cut(s) 208, 659, 995, 1059, 1181
FatI CATG 5 cut(s) 66, 78, 96, 135, 492
FauI CCCGC 2 cut(s) 609, 944
FblI GTMKAC 3 cut(s) 957, 1184, 1280
Fnu4HI GCNGC 8 cut(s) 92, 102, 225, 696, 849, 858, 983, 1003
FokI GGATG 4 cut(s) 155, 173, 800, 881
FriOI GRGCYC 2 cut(s) 8, 315
Fsp4HI GCNGC 8 cut(s) 92, 102, 225, 696, 849, 858, 983, 1003
FspBI CTAG 2 cut(s) 773, 1248
GlaI GCGC 4 cut(s) 534, 812, 887, 1204
GluI GCNGC 8 cut(s) 92, 102, 225, 696, 849, 858, 983, 1003
GsaI CCCAGC 2 cut(s) 892, 952
HaeII RGCGCY 4 cut(s) 536, 814, 889, 1206
HgaI GACGC 3 cut(s) 230, 298, 849
HhaI GCGC 4 cut(s) 535, 813, 888, 1205
Hin1I GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
Hin1II CATG 5 cut(s) 70, 82, 100, 139, 496
Hin6I GCGC 4 cut(s) 533, 811, 886, 1203
HinP1I GCGC 4 cut(s) 533, 811, 886, 1203
HincII GTYRAC 2 cut(s) 63, 1281
HindII GTYRAC 2 cut(s) 63, 1281
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 3 cut(s) 1123, 1142, 1277
HphI GGTGA 2 cut(s) 314, 728
Hpy166II GTNNAC 5 cut(s) 63, 649, 958, 1185, 1281
Hpy188I TCNGA 7 cut(s) 333, 609, 787, 865, 1066, 1087, 1141
Hpy188III TCNNGA 8 cut(s) 234, 251, 286, 308, 720, 773, 911, 1120
Hpy8I GTNNAC 5 cut(s) 63, 649, 958, 1185, 1281
Hpy99I CGWCG 4 cut(s) 179, 275, 287, 749
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4IV ACGT 3 cut(s) 177, 1048, 1082
HpyCH4V TGCA 3 cut(s) 643, 1077, 1110
HpyF10VI GCNNNNNNNGC 8 cut(s) 159, 399, 551, 854, 857, 936, 941, 945
HpyF3I CTNAG 7 cut(s) 107, 293, 404, 536, 1037, 1065, 1272
HpySE526I ACGT 3 cut(s) 177, 1048, 1082
Hsp92I GRCGYC 6 cut(s) 177, 222, 811, 1048, 1082, 1203
Hsp92II CATG 5 cut(s) 70, 82, 100, 139, 496
HspAI GCGC 4 cut(s) 533, 811, 886, 1203
KasI GGCGCC 2 cut(s) 810, 1202
Kpn2I TCCGGA 2 cut(s) 233, 1119
KroI GCCGGC 3 cut(s) 383, 807, 1129
KroNI GCCGGC 3 cut(s) 385, 809, 1131
Kzo9I GATC 5 cut(s) 304, 594, 1216, 1230, 1330
LmnI GCTCC 4 cut(s) 11, 88, 310, 342
Lsp1109I GCAGC 3 cut(s) 78, 994, 1014
LweI GCATC 6 cut(s) 37, 48, 613, 725, 859, 922
MaeI CTAG 2 cut(s) 773, 1248
MaeII ACGT 3 cut(s) 177, 1048, 1082
MaeIII GTNAC 1 cut(s) 1029
MalI GATC 5 cut(s) 306, 596, 1218, 1232, 1332
MboI GATC 5 cut(s) 304, 594, 1216, 1230, 1330
MflI RGATCY 2 cut(s) 1216, 1230
MhlI GDGCHC 3 cut(s) 8, 315, 420
MluCI AATT 2 cut(s) 449, 1115
Mly113I GGCGCC 2 cut(s) 811, 1203
MlyI GAGTC 1 cut(s) 1286
MmeI TCCRAC 2 cut(s) 655, 1110
MroI TCCGGA 2 cut(s) 233, 1119
MroNI GCCGGC 3 cut(s) 383, 807, 1129
MroXI GAANNNNTTC 3 cut(s) 327, 350, 1232
MseI TTAA 1 cut(s) 1098
MslI CAYNNNNRTG 1 cut(s) 497
MspR9I CCNGG 9 cut(s) 299, 482, 506, 572, 800, 815, 979, 1091, 1208
MvaI CCWGG 6 cut(s) 482, 506, 572, 800, 815, 1091
MvnI CGCG 3 cut(s) 851, 860, 912
MwoI GCNNNNNNNGC 8 cut(s) 159, 399, 551, 854, 857, 936, 941, 945
NaeI GCCGGC 3 cut(s) 385, 809, 1131
NarI GGCGCC 2 cut(s) 811, 1203
NciI CCSGG 3 cut(s) 299, 979, 1208
NcoI CCATGG 3 cut(s) 66, 78, 135
NdeII GATC 5 cut(s) 304, 594, 1216, 1230, 1330
NgoMIV GCCGGC 3 cut(s) 383, 807, 1129
NlaIII CATG 5 cut(s) 70, 82, 100, 139, 496
NlaIV GGNNCC 8 cut(s) 163, 312, 616, 675, 729, 812, 1060, 1204
NmeAIII GCCGAG 7 cut(s) 15, 138, 161, 413, 601, 965, 966
NmuCI GTSAC 1 cut(s) 1029
NruI TCGCGA 1 cut(s) 912
NspI RCATGY 1 cut(s) 496
PceI AGGCCT 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 279
PdiI GCCGGC 3 cut(s) 385, 809, 1131
PdmI GAANNNNTTC 3 cut(s) 327, 350, 1232
PfeI GAWTC 2 cut(s) 1123, 1142
PflMI CCANNNNNTGG 2 cut(s) 24, 511
PinAI ACCGGT 1 cut(s) 1055
PkrI GCNGC 8 cut(s) 93, 103, 226, 697, 850, 859, 984, 1004
PleI GAGTC 1 cut(s) 1285
PluTI GGCGCC 2 cut(s) 814, 1206
PpsI GAGTC 1 cut(s) 1285
PpuMI RGGWCCY 1 cut(s) 673
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 6 cut(s) 480, 504, 570, 798, 813, 1089
PspFI CCCAGC 2 cut(s) 888, 948
PspGI CCWGG 6 cut(s) 480, 504, 570, 798, 813, 1089
PspN4I GGNNCC 8 cut(s) 163, 312, 616, 675, 729, 812, 1060, 1204
PspPI GGNCC 9 cut(s) 103, 139, 152, 161, 279, 502, 614, 673, 855
PspPPI RGGWCCY 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 511
PsuI RGATCY 2 cut(s) 1216, 1230
RruI TCGCGA 1 cut(s) 912
RsaI GTAC 3 cut(s) 443, 463, 821
RsaNI GTAC 3 cut(s) 442, 462, 820
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 1279
SaqAI TTAA 1 cut(s) 1098
SatI GCNGC 8 cut(s) 92, 102, 225, 696, 849, 858, 983, 1003
Sau3AI GATC 5 cut(s) 304, 594, 1216, 1230, 1330
Sau96I GGNCC 9 cut(s) 103, 139, 152, 161, 279, 502, 614, 673, 855
SchI GAGTC 1 cut(s) 1286
ScrFI CCNGG 9 cut(s) 299, 482, 506, 572, 800, 815, 979, 1091, 1208
SduI GDGCHC 3 cut(s) 8, 315, 420
SfaNI GCATC 6 cut(s) 37, 48, 613, 725, 859, 922
SfiI GGCCNNNNNGGCC 2 cut(s) 159, 854
SfoI GGCGCC 2 cut(s) 812, 1204
SinI GGWCC 3 cut(s) 139, 614, 673
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 316, 833
SmoI CTYRAG 2 cut(s) 316, 833
Sse9I AATT 2 cut(s) 449, 1115
SseBI AGGCCT 1 cut(s) 425
SspDI GGCGCC 2 cut(s) 810, 1202
SspMI CTAG 2 cut(s) 773, 1248
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 9 cut(s) 297, 480, 504, 570, 798, 813, 977, 1089, 1206
StyI CCWWGG 7 cut(s) 18, 66, 78, 135, 190, 1247, 1285
TaaI ACNGT 1 cut(s) 566
TaiI ACGT 3 cut(s) 180, 1051, 1085
TaqII GACCGA 4 cut(s) 456, 692, 758, 1017
TasI AATT 2 cut(s) 449, 1115
TatI WGTACW 1 cut(s) 461
TauI GCSGC 5 cut(s) 104, 227, 698, 851, 860
TfiI GAWTC 2 cut(s) 1123, 1142
Tru1I TTAA 1 cut(s) 1098
Tru9I TTAA 1 cut(s) 1098
TscAI CASTG 2 cut(s) 516, 927
TseFI GTSAC 1 cut(s) 1029
TseI GCWGC 3 cut(s) 91, 982, 1002
Tsp45I GTSAC 1 cut(s) 1029
TspDTI ATGAA 3 cut(s) 365, 442, 869
TspGWI ACGGA 2 cut(s) 627, 1288
TspRI CASTG 2 cut(s) 516, 927
Van91I CCANNNNNTGG 2 cut(s) 24, 511
VpaK11BI GGWCC 3 cut(s) 139, 614, 673
XagI CCTNNNNNAGG 3 cut(s) 407, 575, 1066
XapI RAATTY 2 cut(s) 449, 1115
XbaI TCTAGA 1 cut(s) 772
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmaJI CCTAGG 1 cut(s) 1247
XmiI GTMKAC 3 cut(s) 957, 1184, 1280
XmnI GAANNNNTTC 3 cut(s) 327, 350, 1232
XspI CTAG 2 cut(s) 773, 1248
ZraI GACGTC 3 cut(s) 178, 1049, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.