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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 676 |
| AccI | GTMKAC | 2 cut(s) | 443, 666 |
| AccII | CGCG | 1 cut(s) | 155 |
| AccIII | TCCGGA | 1 cut(s) | 5 |
| AciI | CCGC | 1 cut(s) | 278 |
| AclWI | GGATC | 1 cut(s) | 99 |
| AcoI | YGGCCR | 1 cut(s) | 454 |
| AcsI | RAATTY | 1 cut(s) | 355 |
| AcuI | CTGAAG | 3 cut(s) | 51, 540, 555 |
| AfaI | GTAC | 2 cut(s) | 288, 632 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 76, 166, 676 |
| AflIII | ACRYGT | 1 cut(s) | 729 |
| AgsI | TTSAA | 9 cut(s) | 128, 179, 206, 271, 284, 398, 472, 514, 655 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 464, 496, 506, 538 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 562, 594 |
| AluBI | AGCT | 5 cut(s) | 56, 175, 323, 533, 606 |
| AluI | AGCT | 5 cut(s) | 56, 175, 323, 533, 606 |
| Alw26I | GTCTC | 1 cut(s) | 368 |
| AlwI | GGATC | 1 cut(s) | 99 |
| Aor13HI | TCCGGA | 1 cut(s) | 5 |
| AoxI | GGCC | 1 cut(s) | 454 |
| ApoI | RAATTY | 1 cut(s) | 355 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 705 |
| AspLEI | GCGC | 1 cut(s) | 68 |
| AspS9I | GGNCC | 1 cut(s) | 711 |
| AsuC2I | CCSGG | 1 cut(s) | 161 |
| AvaII | GGWCC | 1 cut(s) | 711 |
| BalI | TGGCCA | 1 cut(s) | 456 |
| BanII | GRGCYC | 1 cut(s) | 161 |
| BbsI | GAAGAC | 2 cut(s) | 479, 687 |
| BccI | CCATC | 2 cut(s) | 79, 572 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 189, 223 |
| BclI | TGATCA | 1 cut(s) | 49 |
| BcnI | CCSGG | 1 cut(s) | 161 |
| BcoDI | GTCTC | 1 cut(s) | 368 |
| BfaI | CTAG | 4 cut(s) | 143, 255, 377, 597 |
| BglII | AGATCT | 1 cut(s) | 589 |
| BlpI | GCTNAGC | 1 cut(s) | 81 |
| BmcAI | AGTACT | 1 cut(s) | 288 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 1 cut(s) | 711 |
| BmgT120I | GGNCC | 1 cut(s) | 711 |
| BmiI | GGNNCC | 1 cut(s) | 713 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmsI | GCATC | 2 cut(s) | 27, 657 |
| BpiI | GAAGAC | 2 cut(s) | 479, 687 |
| BpmI | CTGGAG | 1 cut(s) | 137 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 81 |
| BpuEI | CTTGAG | 1 cut(s) | 297 |
| BpuMI | CCSGG | 1 cut(s) | 161 |
| BsaJI | CCNNGG | 2 cut(s) | 249, 670 |
| BsaWI | WCCGGW | 1 cut(s) | 5 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 76, 166, 676 |
| Bse118I | RCCGGY | 1 cut(s) | 401 |
| BseAI | TCCGGA | 1 cut(s) | 5 |
| BseDI | CCNNGG | 2 cut(s) | 249, 670 |
| BseGI | GGATG | 2 cut(s) | 42, 564 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 76, 166, 676 |
| BseMII | CTCAG | 1 cut(s) | 72 |
| Bsh1236I | CGCG | 1 cut(s) | 155 |
| BshFI | GGCC | 1 cut(s) | 456 |
| BsiSI | CCGG | 3 cut(s) | 6, 161, 402 |
| BslFI | GGGAC | 1 cut(s) | 697 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 76, 166, 676 |
| BsmAI | GTCTC | 1 cut(s) | 368 |
| BsmFI | GGGAC | 1 cut(s) | 697 |
| BsmI | GAATGC | 1 cut(s) | 15 |
| BsnI | GGCC | 1 cut(s) | 456 |
| Bsp1286I | GDGCHC | 1 cut(s) | 161 |
| Bsp13I | TCCGGA | 1 cut(s) | 5 |
| Bsp143I | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 81 |
| BspACI | CCGC | 1 cut(s) | 278 |
| BspANI | GGCC | 1 cut(s) | 456 |
| BspCNI | CTCAG | 1 cut(s) | 73 |
| BspEI | TCCGGA | 1 cut(s) | 5 |
| BspFNI | CGCG | 1 cut(s) | 155 |
| BspLI | GGNNCC | 1 cut(s) | 713 |
| BspPI | GGATC | 1 cut(s) | 99 |
| BspQI | GCTCTTC | 1 cut(s) | 531 |
| BsrFI | RCCGGY | 1 cut(s) | 401 |
| BssAI | RCCGGY | 1 cut(s) | 401 |
| BssECI | CCNNGG | 2 cut(s) | 249, 670 |
| BssMI | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| BssT1I | CCWWGG | 1 cut(s) | 670 |
| Bst4CI | ACNGT | 1 cut(s) | 441 |
| Bst6I | CTCTTC | 1 cut(s) | 531 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 502 |
| BstC8I | GCNNGC | 1 cut(s) | 157 |
| BstDEI | CTNAG | 1 cut(s) | 81 |
| BstF5I | GGATG | 2 cut(s) | 42, 564 |
| BstFNI | CGCG | 1 cut(s) | 155 |
| BstHHI | GCGC | 1 cut(s) | 68 |
| BstKTI | GATC | 5 cut(s) | 52, 94, 109, 451, 592 |
| BstMAI | GTCTC | 1 cut(s) | 368 |
| BstMBI | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 502 |
| BstNSI | RCATGY | 1 cut(s) | 733 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstUI | CGCG | 1 cut(s) | 155 |
| BstV2I | GAAGAC | 2 cut(s) | 479, 687 |
| BstX2I | RGATCY | 1 cut(s) | 589 |
| BstYI | RGATCY | 1 cut(s) | 589 |
| BsuRI | GGCC | 1 cut(s) | 456 |
| BtsCI | GGATG | 2 cut(s) | 42, 564 |
| Cac8I | GCNNGC | 1 cut(s) | 157 |
| CfoI | GCGC | 1 cut(s) | 68 |
| Cfr10I | RCCGGY | 1 cut(s) | 401 |
| Cfr13I | GGNCC | 1 cut(s) | 711 |
| Csp6I | GTAC | 2 cut(s) | 287, 631 |
| CviAII | CATG | 2 cut(s) | 335, 730 |
| CviQI | GTAC | 2 cut(s) | 287, 631 |
| DdeI | CTNAG | 1 cut(s) | 81 |
| DpnI | GATC | 5 cut(s) | 51, 93, 108, 450, 591 |
| DpnII | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| EaeI | YGGCCR | 1 cut(s) | 454 |
| Eam1104I | CTCTTC | 1 cut(s) | 531 |
| EarI | CTCTTC | 1 cut(s) | 531 |
| Eco130I | CCWWGG | 1 cut(s) | 670 |
| Eco24I | GRGCYC | 1 cut(s) | 161 |
| Eco47I | GGWCC | 1 cut(s) | 711 |
| Eco57I | CTGAAG | 3 cut(s) | 51, 540, 555 |
| EcoT14I | CCWWGG | 1 cut(s) | 670 |
| EcoT22I | ATGCAT | 1 cut(s) | 650 |
| EcoT38I | GRGCYC | 1 cut(s) | 161 |
| ErhI | CCWWGG | 1 cut(s) | 670 |
| FaeI | CATG | 2 cut(s) | 338, 733 |
| FaiI | YATR | 7 cut(s) | 327, 336, 426, 465, 603, 677, 731 |
| FaqI | GGGAC | 1 cut(s) | 697 |
| FatI | CATG | 2 cut(s) | 334, 729 |
| FbaI | TGATCA | 1 cut(s) | 49 |
| FblI | GTMKAC | 2 cut(s) | 443, 666 |
| FokI | GGATG | 2 cut(s) | 49, 551 |
| FriOI | GRGCYC | 1 cut(s) | 161 |
| FspBI | CTAG | 4 cut(s) | 143, 255, 377, 597 |
| GlaI | GCGC | 1 cut(s) | 67 |
| GsuI | CTGGAG | 1 cut(s) | 137 |
| HaeIII | GGCC | 1 cut(s) | 456 |
| HapII | CCGG | 3 cut(s) | 6, 161, 402 |
| HhaI | GCGC | 1 cut(s) | 68 |
| Hin1II | CATG | 2 cut(s) | 338, 733 |
| Hin6I | GCGC | 1 cut(s) | 66 |
| HinP1I | GCGC | 1 cut(s) | 66 |
| HincII | GTYRAC | 1 cut(s) | 667 |
| HindII | GTYRAC | 1 cut(s) | 667 |
| HindIII | AAGCTT | 2 cut(s) | 173, 321 |
| HinfI | GANTC | 1 cut(s) | 206 |
| HpaII | CCGG | 3 cut(s) | 6, 161, 402 |
| Hpy166II | GTNNAC | 2 cut(s) | 444, 667 |
| Hpy188I | TCNGA | 3 cut(s) | 215, 569, 727 |
| Hpy188III | TCNNGA | 4 cut(s) | 6, 116, 134, 652 |
| Hpy8I | GTNNAC | 2 cut(s) | 444, 667 |
| Hpy99I | CGWCG | 1 cut(s) | 79 |
| HpyAV | CCTTC | 3 cut(s) | 26, 190, 486 |
| HpyCH4III | ACNGT | 1 cut(s) | 441 |
| HpyCH4V | TGCA | 2 cut(s) | 353, 648 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 502 |
| HpyF3I | CTNAG | 1 cut(s) | 81 |
| Hsp92II | CATG | 2 cut(s) | 338, 733 |
| HspAI | GCGC | 1 cut(s) | 66 |
| Kpn2I | TCCGGA | 1 cut(s) | 5 |
| Ksp22I | TGATCA | 1 cut(s) | 49 |
| Kzo9I | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| LguI | GCTCTTC | 1 cut(s) | 531 |
| LpnPI | CCDG | 7 cut(s) | 19, 99, 101, 174, 361, 385, 415 |
| LweI | GCATC | 2 cut(s) | 27, 657 |
| MaeI | CTAG | 4 cut(s) | 143, 255, 377, 597 |
| MaeIII | GTNAC | 2 cut(s) | 120, 244 |
| MalI | GATC | 5 cut(s) | 51, 93, 108, 450, 591 |
| MboI | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| MboII | GAAGA | 5 cut(s) | 484, 548, 652, 692, 698 |
| MfeI | CAATTG | 1 cut(s) | 521 |
| MflI | RGATCY | 1 cut(s) | 589 |
| MhlI | GDGCHC | 1 cut(s) | 161 |
| MlsI | TGGCCA | 1 cut(s) | 456 |
| MluCI | AATT | 5 cut(s) | 128, 218, 355, 410, 521 |
| MluNI | TGGCCA | 1 cut(s) | 456 |
| Mox20I | TGGCCA | 1 cut(s) | 456 |
| Mph1103I | ATGCAT | 1 cut(s) | 650 |
| MroI | TCCGGA | 1 cut(s) | 5 |
| MroXI | GAANNNNTTC | 1 cut(s) | 705 |
| MscI | TGGCCA | 1 cut(s) | 456 |
| MseI | TTAA | 1 cut(s) | 696 |
| Msp20I | TGGCCA | 1 cut(s) | 456 |
| MspI | CCGG | 3 cut(s) | 6, 161, 402 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| MunI | CAATTG | 1 cut(s) | 521 |
| Mva1269I | GAATGC | 1 cut(s) | 15 |
| MvnI | CGCG | 1 cut(s) | 155 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 502 |
| NciI | CCSGG | 1 cut(s) | 161 |
| NdeII | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| NlaIII | CATG | 2 cut(s) | 338, 733 |
| NlaIV | GGNNCC | 1 cut(s) | 713 |
| NsiI | ATGCAT | 1 cut(s) | 650 |
| NspI | RCATGY | 1 cut(s) | 733 |
| PciI | ACATGT | 1 cut(s) | 729 |
| PciSI | GCTCTTC | 1 cut(s) | 531 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 626 |
| PctI | GAATGC | 1 cut(s) | 15 |
| PdmI | GAANNNNTTC | 1 cut(s) | 705 |
| PfeI | GAWTC | 1 cut(s) | 206 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 676 |
| PscI | ACATGT | 1 cut(s) | 729 |
| PspN4I | GGNNCC | 1 cut(s) | 713 |
| PspPI | GGNCC | 1 cut(s) | 711 |
| PsuI | RGATCY | 1 cut(s) | 589 |
| RsaI | GTAC | 2 cut(s) | 288, 632 |
| RsaNI | GTAC | 2 cut(s) | 287, 631 |
| SalI | GTCGAC | 1 cut(s) | 665 |
| SapI | GCTCTTC | 1 cut(s) | 531 |
| SaqAI | TTAA | 1 cut(s) | 696 |
| Sau3AI | GATC | 5 cut(s) | 49, 91, 106, 448, 589 |
| Sau96I | GGNCC | 1 cut(s) | 711 |
| ScaI | AGTACT | 1 cut(s) | 288 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SduI | GDGCHC | 1 cut(s) | 161 |
| SfaNI | GCATC | 2 cut(s) | 27, 657 |
| SinI | GGWCC | 1 cut(s) | 711 |
| SmlI | CTYRAG | 1 cut(s) | 312 |
| SmoI | CTYRAG | 1 cut(s) | 312 |
| Sse9I | AATT | 5 cut(s) | 128, 218, 355, 410, 521 |
| SsiI | CCGC | 1 cut(s) | 278 |
| SspMI | CTAG | 4 cut(s) | 143, 255, 377, 597 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| StyI | CCWWGG | 1 cut(s) | 670 |
| TaaI | ACNGT | 1 cut(s) | 441 |
| TaqI | TCGA | 5 cut(s) | 43, 135, 436, 528, 666 |
| TaqII | GACCGA | 3 cut(s) | 199, 610, 699 |
| TasI | AATT | 5 cut(s) | 128, 218, 355, 410, 521 |
| TatI | WGTACW | 1 cut(s) | 286 |
| TfiI | GAWTC | 1 cut(s) | 206 |
| Tru1I | TTAA | 1 cut(s) | 696 |
| Tru9I | TTAA | 1 cut(s) | 696 |
| TspDTI | ATGAA | 3 cut(s) | 323, 342, 452 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 676 |
| VpaK11BI | GGWCC | 1 cut(s) | 711 |
| XapI | RAATTY | 1 cut(s) | 355 |
| XceI | RCATGY | 1 cut(s) | 733 |
| XmiI | GTMKAC | 2 cut(s) | 443, 666 |
| XmnI | GAANNNNTTC | 1 cut(s) | 705 |
| XspI | CTAG | 4 cut(s) | 143, 255, 377, 597 |
| ZrmI | AGTACT | 1 cut(s) | 288 |
| Zsp2I | ATGCAT | 1 cut(s) | 650 |