No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 306 |
| AciI | CCGC | 4 cut(s) | 304, 321, 401, 419 |
| AclWI | GGATC | 1 cut(s) | 266 |
| AcoI | YGGCCR | 3 cut(s) | 78, 192, 402 |
| AcuI | CTGAAG | 2 cut(s) | 115, 347 |
| AcyI | GRCGYC | 1 cut(s) | 267 |
| AfaI | GTAC | 1 cut(s) | 42 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 32, 425 |
| AjnI | CCWGG | 2 cut(s) | 169, 181 |
| AluBI | AGCT | 4 cut(s) | 126, 277, 361, 492 |
| AluI | AGCT | 4 cut(s) | 126, 277, 361, 492 |
| Alw21I | GWGCWC | 1 cut(s) | 376 |
| Alw44I | GTGCAC | 1 cut(s) | 372 |
| AlwI | GGATC | 1 cut(s) | 266 |
| AoxI | GGCC | 6 cut(s) | 52, 78, 179, 192, 402, 412 |
| ApaLI | GTGCAC | 1 cut(s) | 372 |
| ApeKI | GCWGC | 1 cut(s) | 317 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 93 |
| AsuHPI | GGTGA | 1 cut(s) | 197 |
| BaeGI | GKGCMC | 1 cut(s) | 376 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 24, 56 |
| Bbv12I | GWGCWC | 1 cut(s) | 376 |
| BbvI | GCAGC | 1 cut(s) | 304 |
| BccI | CCATC | 1 cut(s) | 278 |
| BceAI | ACGGC | 1 cut(s) | 207 |
| BciT130I | CCWGG | 2 cut(s) | 171, 183 |
| BfaI | CTAG | 1 cut(s) | 123 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 427 |
| BisI | GCNGC | 4 cut(s) | 318, 321, 402, 419 |
| BlsI | GCNGC | 4 cut(s) | 319, 322, 403, 420 |
| Bme1390I | CCNGG | 2 cut(s) | 171, 183 |
| BmrFI | CCNGG | 2 cut(s) | 171, 183 |
| BpmI | CTGGAG | 1 cut(s) | 377 |
| BsaHI | GRCGYC | 1 cut(s) | 267 |
| BsaJI | CCNNGG | 1 cut(s) | 206 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 350, 380 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 32, 425 |
| Bse118I | RCCGGY | 1 cut(s) | 76 |
| Bse1I | ACTGG | 1 cut(s) | 203 |
| BseBI | CCWGG | 2 cut(s) | 171, 183 |
| BseDI | CCNNGG | 1 cut(s) | 206 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 32, 425 |
| BseMII | CTCAG | 1 cut(s) | 89 |
| BseNI | ACTGG | 1 cut(s) | 203 |
| BseSI | GKGCMC | 1 cut(s) | 376 |
| BseX3I | CGGCCG | 1 cut(s) | 78 |
| BseXI | GCAGC | 1 cut(s) | 304 |
| Bsh1285I | CGRYCG | 1 cut(s) | 81 |
| BshFI | GGCC | 6 cut(s) | 54, 80, 181, 194, 404, 414 |
| BsiEI | CGRYCG | 1 cut(s) | 81 |
| BsiHKAI | GWGCWC | 1 cut(s) | 376 |
| BsiSI | CCGG | 1 cut(s) | 77 |
| BslFI | GGGAC | 1 cut(s) | 8 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 32, 425 |
| BsmFI | GGGAC | 1 cut(s) | 8 |
| BsnI | GGCC | 6 cut(s) | 54, 80, 181, 194, 404, 414 |
| Bsp1286I | GDGCHC | 1 cut(s) | 376 |
| Bsp143I | GATC | 2 cut(s) | 258, 477 |
| BspACI | CCGC | 4 cut(s) | 304, 321, 401, 419 |
| BspANI | GGCC | 6 cut(s) | 54, 80, 181, 194, 404, 414 |
| BspCNI | CTCAG | 1 cut(s) | 90 |
| BspPI | GGATC | 1 cut(s) | 266 |
| BsrBI | CCGCTC | 1 cut(s) | 306 |
| BsrFI | RCCGGY | 1 cut(s) | 76 |
| BsrI | ACTGG | 1 cut(s) | 203 |
| BssAI | RCCGGY | 1 cut(s) | 76 |
| BssECI | CCNNGG | 1 cut(s) | 206 |
| BssMI | GATC | 2 cut(s) | 258, 477 |
| BssNI | GRCGYC | 1 cut(s) | 267 |
| Bst2UI | CCWGG | 2 cut(s) | 171, 183 |
| BstACI | GRCGYC | 1 cut(s) | 267 |
| BstC8I | GCNNGC | 4 cut(s) | 104, 315, 416, 490 |
| BstDEI | CTNAG | 3 cut(s) | 56, 98, 377 |
| BstDSI | CCRYGG | 1 cut(s) | 206 |
| BstKTI | GATC | 2 cut(s) | 261, 480 |
| BstMBI | GATC | 2 cut(s) | 258, 477 |
| BstMCI | CGRYCG | 1 cut(s) | 81 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 152, 427 |
| BstNI | CCWGG | 2 cut(s) | 171, 183 |
| BstSCI | CCNGG | 2 cut(s) | 169, 181 |
| BstSLI | GKGCMC | 1 cut(s) | 376 |
| BstV1I | GCAGC | 1 cut(s) | 304 |
| BstX2I | RGATCY | 1 cut(s) | 258 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 177 |
| BstYI | RGATCY | 1 cut(s) | 258 |
| BstZI | CGGCCG | 1 cut(s) | 78 |
| BsuRI | GGCC | 6 cut(s) | 54, 80, 181, 194, 404, 414 |
| BtgI | CCRYGG | 1 cut(s) | 206 |
| BtsIMutI | CAGTG | 1 cut(s) | 139 |
| Cac8I | GCNNGC | 4 cut(s) | 104, 315, 416, 490 |
| Cfr10I | RCCGGY | 1 cut(s) | 76 |
| CseI | GACGC | 1 cut(s) | 275 |
| Csp6I | GTAC | 1 cut(s) | 41 |
| CviAII | CATG | 4 cut(s) | 107, 147, 156, 425 |
| CviQI | GTAC | 1 cut(s) | 41 |
| DdeI | CTNAG | 3 cut(s) | 56, 98, 377 |
| DpnI | GATC | 2 cut(s) | 260, 479 |
| DpnII | GATC | 2 cut(s) | 258, 477 |
| EaeI | YGGCCR | 3 cut(s) | 78, 192, 402 |
| EagI | CGGCCG | 1 cut(s) | 78 |
| EclXI | CGGCCG | 1 cut(s) | 78 |
| Eco147I | AGGCCT | 1 cut(s) | 414 |
| Eco52I | CGGCCG | 1 cut(s) | 78 |
| Eco57I | CTGAAG | 2 cut(s) | 115, 347 |
| EcoRII | CCWGG | 2 cut(s) | 169, 181 |
| FaeI | CATG | 4 cut(s) | 110, 150, 159, 428 |
| FaqI | GGGAC | 1 cut(s) | 8 |
| FatI | CATG | 4 cut(s) | 106, 146, 155, 424 |
| Fnu4HI | GCNGC | 4 cut(s) | 318, 321, 402, 419 |
| Fsp4HI | GCNGC | 4 cut(s) | 318, 321, 402, 419 |
| FspBI | CTAG | 1 cut(s) | 123 |
| GluI | GCNGC | 4 cut(s) | 318, 321, 402, 419 |
| GsuI | CTGGAG | 1 cut(s) | 377 |
| HaeIII | GGCC | 6 cut(s) | 54, 80, 181, 194, 404, 414 |
| HapII | CCGG | 1 cut(s) | 77 |
| HgaI | GACGC | 1 cut(s) | 275 |
| Hin1I | GRCGYC | 1 cut(s) | 267 |
| Hin1II | CATG | 4 cut(s) | 110, 150, 159, 428 |
| HincII | GTYRAC | 1 cut(s) | 47 |
| HindII | GTYRAC | 1 cut(s) | 47 |
| HinfI | GANTC | 3 cut(s) | 21, 119, 202 |
| HpaII | CCGG | 1 cut(s) | 77 |
| HphI | GGTGA | 1 cut(s) | 197 |
| Hpy166II | GTNNAC | 4 cut(s) | 47, 282, 374, 439 |
| Hpy188I | TCNGA | 3 cut(s) | 99, 384, 454 |
| Hpy188III | TCNNGA | 3 cut(s) | 25, 223, 356 |
| Hpy8I | GTNNAC | 4 cut(s) | 47, 282, 374, 439 |
| HpyAV | CCTTC | 3 cut(s) | 26, 131, 449 |
| HpyCH4IV | ACGT | 1 cut(s) | 43 |
| HpyCH4V | TGCA | 1 cut(s) | 374 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 152, 427 |
| HpyF3I | CTNAG | 3 cut(s) | 56, 98, 377 |
| HpySE526I | ACGT | 1 cut(s) | 43 |
| Hsp92I | GRCGYC | 1 cut(s) | 267 |
| Hsp92II | CATG | 4 cut(s) | 110, 150, 159, 428 |
| Kzo9I | GATC | 2 cut(s) | 258, 477 |
| LmnI | GCTCC | 3 cut(s) | 152, 311, 358 |
| Lsp1109I | GCAGC | 1 cut(s) | 304 |
| MaeI | CTAG | 1 cut(s) | 123 |
| MaeII | ACGT | 1 cut(s) | 43 |
| MaeIII | GTNAC | 1 cut(s) | 299 |
| MalI | GATC | 2 cut(s) | 260, 479 |
| MbiI | CCGCTC | 1 cut(s) | 306 |
| MboI | GATC | 2 cut(s) | 258, 477 |
| MflI | RGATCY | 1 cut(s) | 258 |
| MhlI | GDGCHC | 1 cut(s) | 376 |
| MluCI | AATT | 1 cut(s) | 215 |
| MlyI | GAGTC | 1 cut(s) | 30 |
| MmeI | TCCRAC | 1 cut(s) | 407 |
| MroXI | GAANNNNTTC | 1 cut(s) | 93 |
| MspI | CCGG | 1 cut(s) | 77 |
| MspR9I | CCNGG | 2 cut(s) | 171, 183 |
| MvaI | CCWGG | 2 cut(s) | 171, 183 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 152, 427 |
| NdeII | GATC | 2 cut(s) | 258, 477 |
| NlaIII | CATG | 4 cut(s) | 110, 150, 159, 428 |
| NmuCI | GTSAC | 1 cut(s) | 299 |
| PceI | AGGCCT | 1 cut(s) | 414 |
| PdmI | GAANNNNTTC | 1 cut(s) | 93 |
| PfeI | GAWTC | 2 cut(s) | 119, 202 |
| PkrI | GCNGC | 4 cut(s) | 319, 322, 403, 420 |
| PleI | GAGTC | 1 cut(s) | 29 |
| PpsI | GAGTC | 1 cut(s) | 29 |
| Psp6I | CCWGG | 2 cut(s) | 169, 181 |
| PspGI | CCWGG | 2 cut(s) | 169, 181 |
| PsuI | RGATCY | 1 cut(s) | 258 |
| RsaI | GTAC | 1 cut(s) | 42 |
| RsaNI | GTAC | 1 cut(s) | 41 |
| SatI | GCNGC | 4 cut(s) | 318, 321, 402, 419 |
| Sau3AI | GATC | 2 cut(s) | 258, 477 |
| SchI | GAGTC | 1 cut(s) | 30 |
| ScrFI | CCNGG | 2 cut(s) | 171, 183 |
| SduI | GDGCHC | 1 cut(s) | 376 |
| SetI | ASST | 7 cut(s) | 37, 46, 128, 279, 363, 389, 494 |
| Sse9I | AATT | 1 cut(s) | 215 |
| SseBI | AGGCCT | 1 cut(s) | 414 |
| SsiI | CCGC | 4 cut(s) | 304, 321, 401, 419 |
| SspMI | CTAG | 1 cut(s) | 123 |
| StuI | AGGCCT | 1 cut(s) | 414 |
| StyD4I | CCNGG | 2 cut(s) | 169, 181 |
| TaiI | ACGT | 1 cut(s) | 46 |
| TaqI | TCGA | 1 cut(s) | 222 |
| TasI | AATT | 1 cut(s) | 215 |
| TauI | GCSGC | 3 cut(s) | 323, 404, 421 |
| TfiI | GAWTC | 2 cut(s) | 119, 202 |
| TscAI | CASTG | 1 cut(s) | 139 |
| TseFI | GTSAC | 1 cut(s) | 299 |
| TseI | GCWGC | 1 cut(s) | 317 |
| Tsp45I | GTSAC | 1 cut(s) | 299 |
| TspDTI | ATGAA | 3 cut(s) | 17, 45, 123 |
| TspGWI | ACGGA | 1 cut(s) | 195 |
| TspRI | CASTG | 1 cut(s) | 139 |
| VneI | GTGCAC | 1 cut(s) | 372 |
| XmnI | GAANNNNTTC | 1 cut(s) | 93 |
| XspI | CTAG | 1 cut(s) | 123 |