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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 77 |
| AfaI | GTAC | 1 cut(s) | 162 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 152 |
| Alw26I | GTCTC | 1 cut(s) | 115 |
| AoxI | GGCC | 2 cut(s) | 114, 172 |
| AspS9I | GGNCC | 2 cut(s) | 15, 173 |
| AsuHPI | GGTGA | 1 cut(s) | 46 |
| AsuII | TTCGAA | 1 cut(s) | 8 |
| AvaII | GGWCC | 1 cut(s) | 15 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 144, 176 |
| BbsI | GAAGAC | 1 cut(s) | 30 |
| BcoDI | GTCTC | 1 cut(s) | 115 |
| BfaI | CTAG | 1 cut(s) | 220 |
| Bme18I | GGWCC | 1 cut(s) | 15 |
| BmgT120I | GGNCC | 2 cut(s) | 15, 173 |
| BmiI | GGNNCC | 1 cut(s) | 16 |
| BpiI | GAAGAC | 1 cut(s) | 30 |
| Bpu14I | TTCGAA | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 1 cut(s) | 211 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 152 |
| BseDI | CCNNGG | 1 cut(s) | 211 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BshFI | GGCC | 2 cut(s) | 116, 174 |
| BslFI | GGGAC | 2 cut(s) | 28, 128 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BsmAI | GTCTC | 1 cut(s) | 115 |
| BsmFI | GGGAC | 2 cut(s) | 28, 128 |
| BsnI | GGCC | 2 cut(s) | 116, 174 |
| Bsp119I | TTCGAA | 1 cut(s) | 8 |
| BspACI | CCGC | 1 cut(s) | 77 |
| BspANI | GGCC | 2 cut(s) | 116, 174 |
| BspHI | TCATGA | 1 cut(s) | 120 |
| BspLI | GGNNCC | 1 cut(s) | 16 |
| BspT104I | TTCGAA | 1 cut(s) | 8 |
| BssECI | CCNNGG | 1 cut(s) | 211 |
| BstBI | TTCGAA | 1 cut(s) | 8 |
| BstDEI | CTNAG | 1 cut(s) | 86 |
| BstDSI | CCRYGG | 1 cut(s) | 211 |
| BstMAI | GTCTC | 1 cut(s) | 115 |
| BstV2I | GAAGAC | 1 cut(s) | 30 |
| BsuRI | GGCC | 2 cut(s) | 116, 174 |
| BtgI | CCRYGG | 1 cut(s) | 211 |
| BtsIMutI | CAGTG | 1 cut(s) | 96 |
| CciI | TCATGA | 1 cut(s) | 120 |
| Cfr13I | GGNCC | 2 cut(s) | 15, 173 |
| Csp6I | GTAC | 1 cut(s) | 161 |
| CviAII | CATG | 1 cut(s) | 121 |
| CviJI | RGCY | 4 cut(s) | 5, 95, 116, 174 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 95, 116, 174 |
| CviQI | GTAC | 1 cut(s) | 161 |
| DdeI | CTNAG | 1 cut(s) | 86 |
| Eco47I | GGWCC | 1 cut(s) | 15 |
| FaeI | CATG | 1 cut(s) | 124 |
| FaiI | YATR | 4 cut(s) | 33, 122, 186, 194 |
| FaqI | GGGAC | 2 cut(s) | 28, 128 |
| FatI | CATG | 1 cut(s) | 120 |
| FspBI | CTAG | 1 cut(s) | 220 |
| HaeIII | GGCC | 2 cut(s) | 116, 174 |
| Hin1II | CATG | 1 cut(s) | 124 |
| HincII | GTYRAC | 1 cut(s) | 167 |
| HindII | GTYRAC | 1 cut(s) | 167 |
| HinfI | GANTC | 3 cut(s) | 141, 199, 216 |
| HphI | GGTGA | 1 cut(s) | 46 |
| Hpy166II | GTNNAC | 1 cut(s) | 167 |
| Hpy188III | TCNNGA | 2 cut(s) | 121, 145 |
| Hpy8I | GTNNAC | 1 cut(s) | 167 |
| HpyAV | CCTTC | 3 cut(s) | 29, 127, 146 |
| HpyCH4IV | ACGT | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 1 cut(s) | 71 |
| HpyF3I | CTNAG | 1 cut(s) | 86 |
| HpySE526I | ACGT | 1 cut(s) | 163 |
| Hsp92II | CATG | 1 cut(s) | 124 |
| MaeI | CTAG | 1 cut(s) | 220 |
| MaeII | ACGT | 1 cut(s) | 163 |
| MboII | GAAGA | 2 cut(s) | 32, 35 |
| MluCI | AATT | 1 cut(s) | 28 |
| MlyI | GAGTC | 2 cut(s) | 150, 193 |
| NlaIII | CATG | 1 cut(s) | 124 |
| NlaIV | GGNNCC | 1 cut(s) | 16 |
| NmeAIII | GCCGAG | 1 cut(s) | 92 |
| NspV | TTCGAA | 1 cut(s) | 8 |
| PagI | TCATGA | 1 cut(s) | 120 |
| PfeI | GAWTC | 1 cut(s) | 216 |
| PleI | GAGTC | 2 cut(s) | 149, 193 |
| PpsI | GAGTC | 2 cut(s) | 149, 193 |
| PspN4I | GGNNCC | 1 cut(s) | 16 |
| PspPI | GGNCC | 2 cut(s) | 15, 173 |
| RsaI | GTAC | 1 cut(s) | 162 |
| RsaNI | GTAC | 1 cut(s) | 161 |
| Sau96I | GGNCC | 2 cut(s) | 15, 173 |
| SchI | GAGTC | 2 cut(s) | 150, 193 |
| SetI | ASST | 2 cut(s) | 157, 166 |
| SfuI | TTCGAA | 1 cut(s) | 8 |
| SgeI | CNNG | 8 cut(s) | 104, 124, 133, 152, 157, 182, 189, 213 |
| SinI | GGWCC | 1 cut(s) | 15 |
| Sse9I | AATT | 1 cut(s) | 28 |
| SsiI | CCGC | 1 cut(s) | 77 |
| SspMI | CTAG | 1 cut(s) | 220 |
| TaiI | ACGT | 1 cut(s) | 166 |
| TaqI | TCGA | 1 cut(s) | 8 |
| TaqII | GACCGA | 1 cut(s) | 32 |
| TasI | AATT | 1 cut(s) | 28 |
| TfiI | GAWTC | 1 cut(s) | 216 |
| TscAI | CASTG | 1 cut(s) | 103 |
| TspDTI | ATGAA | 4 cut(s) | 48, 109, 137, 165 |
| TspRI | CASTG | 1 cut(s) | 103 |
| VpaK11BI | GGWCC | 1 cut(s) | 15 |
| XspI | CTAG | 1 cut(s) | 220 |