Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 29 |
| AccII | CGCG | 1 cut(s) | 78 |
| AciI | CCGC | 1 cut(s) | 78 |
| AcoI | YGGCCR | 3 cut(s) | 220, 415, 475 |
| AcsI | RAATTY | 1 cut(s) | 688 |
| AcuI | CTGAAG | 1 cut(s) | 477 |
| AfaI | GTAC | 4 cut(s) | 231, 274, 486, 529 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 286, 326, 353, 541, 581, 608 |
| AgsI | TTSAA | 1 cut(s) | 687 |
| AjnI | CCWGG | 2 cut(s) | 279, 534 |
| AluBI | AGCT | 3 cut(s) | 64, 632, 661 |
| AluI | AGCT | 3 cut(s) | 64, 632, 661 |
| Alw21I | GWGCWC | 1 cut(s) | 219 |
| Alw26I | GTCTC | 2 cut(s) | 181, 436 |
| Ama87I | CYCGRG | 2 cut(s) | 151, 406 |
| AoxI | GGCC | 4 cut(s) | 93, 220, 415, 475 |
| ApoI | RAATTY | 1 cut(s) | 688 |
| AspS9I | GGNCC | 1 cut(s) | 387 |
| AsuHPI | GGTGA | 4 cut(s) | 109, 236, 491, 679 |
| AvaI | CYCGRG | 2 cut(s) | 151, 406 |
| AvaII | GGWCC | 1 cut(s) | 387 |
| BalI | TGGCCA | 1 cut(s) | 477 |
| BanI | GGYRCC | 1 cut(s) | 29 |
| Bbv12I | GWGCWC | 1 cut(s) | 219 |
| BccI | CCATC | 4 cut(s) | 249, 399, 504, 655 |
| BciT130I | CCWGG | 2 cut(s) | 281, 536 |
| BcoDI | GTCTC | 2 cut(s) | 181, 436 |
| BfmI | CTRYAG | 1 cut(s) | 633 |
| BisI | GCNGC | 1 cut(s) | 79 |
| BlsI | GCNGC | 1 cut(s) | 80 |
| BmcAI | AGTACT | 2 cut(s) | 231, 486 |
| Bme1390I | CCNGG | 2 cut(s) | 281, 536 |
| Bme18I | GGWCC | 1 cut(s) | 387 |
| BmeT110I | CYCGRG | 2 cut(s) | 151, 406 |
| BmgT120I | GGNCC | 1 cut(s) | 387 |
| BmiI | GGNNCC | 3 cut(s) | 31, 388, 389 |
| BmrFI | CCNGG | 2 cut(s) | 281, 536 |
| BmsI | GCATC | 2 cut(s) | 165, 420 |
| BpmI | CTGGAG | 2 cut(s) | 302, 557 |
| BsaJI | CCNNGG | 5 cut(s) | 150, 279, 405, 447, 534 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 286, 326, 353, 541, 581, 608 |
| Bse3DI | GCAATG | 1 cut(s) | 42 |
| BseBI | CCWGG | 2 cut(s) | 281, 536 |
| BseDI | CCNNGG | 5 cut(s) | 150, 279, 405, 447, 534 |
| BseGI | GGATG | 2 cut(s) | 363, 618 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 286, 326, 353, 541, 581, 608 |
| BseMI | GCAATG | 1 cut(s) | 42 |
| BseRI | GAGGAG | 2 cut(s) | 56, 59 |
| Bsh1236I | CGCG | 1 cut(s) | 78 |
| BshFI | GGCC | 4 cut(s) | 95, 222, 417, 477 |
| BshNI | GGYRCC | 1 cut(s) | 29 |
| BsiHKAI | GWGCWC | 1 cut(s) | 219 |
| BsiHKCI | CYCGRG | 2 cut(s) | 151, 406 |
| BsiSI | CCGG | 1 cut(s) | 36 |
| BslFI | GGGAC | 2 cut(s) | 145, 400 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 286, 326, 353, 541, 581, 608 |
| BsmAI | GTCTC | 2 cut(s) | 181, 436 |
| BsmFI | GGGAC | 2 cut(s) | 145, 400 |
| BsnI | GGCC | 4 cut(s) | 95, 222, 417, 477 |
| BsoBI | CYCGRG | 2 cut(s) | 151, 406 |
| Bsp1286I | GDGCHC | 1 cut(s) | 219 |
| BspACI | CCGC | 1 cut(s) | 78 |
| BspANI | GGCC | 4 cut(s) | 95, 222, 417, 477 |
| BspFNI | CGCG | 1 cut(s) | 78 |
| BspLI | GGNNCC | 3 cut(s) | 31, 388, 389 |
| BspQI | GCTCTTC | 2 cut(s) | 297, 552 |
| BspT107I | GGYRCC | 1 cut(s) | 29 |
| BsrDI | GCAATG | 1 cut(s) | 42 |
| BssECI | CCNNGG | 5 cut(s) | 150, 279, 405, 447, 534 |
| BssT1I | CCWWGG | 1 cut(s) | 447 |
| Bst2UI | CCWGG | 2 cut(s) | 281, 536 |
| Bst4CI | ACNGT | 6 cut(s) | 88, 175, 188, 430, 443, 637 |
| Bst6I | CTCTTC | 4 cut(s) | 297, 348, 552, 603 |
| BstC8I | GCNNGC | 1 cut(s) | 630 |
| BstDEI | CTNAG | 1 cut(s) | 192 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 351, 606 |
| BstF5I | GGATG | 2 cut(s) | 363, 618 |
| BstFNI | CGCG | 1 cut(s) | 78 |
| BstMAI | GTCTC | 2 cut(s) | 181, 436 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 61 |
| BstNI | CCWGG | 2 cut(s) | 281, 536 |
| BstSCI | CCNGG | 2 cut(s) | 279, 534 |
| BstSFI | CTRYAG | 1 cut(s) | 633 |
| BstUI | CGCG | 1 cut(s) | 78 |
| BsuRI | GGCC | 4 cut(s) | 95, 222, 417, 477 |
| BtsCI | GGATG | 2 cut(s) | 363, 618 |
| BtsIMutI | CAGTG | 1 cut(s) | 84 |
| Cac8I | GCNNGC | 1 cut(s) | 630 |
| Cfr13I | GGNCC | 1 cut(s) | 387 |
| CseI | GACGC | 1 cut(s) | 10 |
| Csp6I | GTAC | 4 cut(s) | 230, 273, 485, 528 |
| CviAII | CATG | 1 cut(s) | 51 |
| CviQI | GTAC | 4 cut(s) | 230, 273, 485, 528 |
| DdeI | CTNAG | 1 cut(s) | 192 |
| EaeI | YGGCCR | 3 cut(s) | 220, 415, 475 |
| Eam1104I | CTCTTC | 4 cut(s) | 297, 348, 552, 603 |
| EarI | CTCTTC | 4 cut(s) | 297, 348, 552, 603 |
| Eco130I | CCWWGG | 1 cut(s) | 447 |
| Eco147I | AGGCCT | 1 cut(s) | 95 |
| Eco32I | GATATC | 1 cut(s) | 463 |
| Eco47I | GGWCC | 1 cut(s) | 387 |
| Eco57I | CTGAAG | 1 cut(s) | 477 |
| Eco88I | CYCGRG | 2 cut(s) | 151, 406 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 351, 606 |
| EcoO109I | RGGNCCY | 1 cut(s) | 387 |
| EcoRII | CCWGG | 2 cut(s) | 279, 534 |
| EcoRV | GATATC | 1 cut(s) | 463 |
| EcoT14I | CCWWGG | 1 cut(s) | 447 |
| ErhI | CCWWGG | 1 cut(s) | 447 |
| FaeI | CATG | 1 cut(s) | 54 |
| FaiI | YATR | 4 cut(s) | 52, 207, 323, 578 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 219, 251, 474, 506 |
| FaqI | GGGAC | 2 cut(s) | 145, 400 |
| FatI | CATG | 1 cut(s) | 50 |
| Fnu4HI | GCNGC | 1 cut(s) | 79 |
| FokI | GGATG | 2 cut(s) | 370, 625 |
| Fsp4HI | GCNGC | 1 cut(s) | 79 |
| GluI | GCNGC | 1 cut(s) | 79 |
| GsuI | CTGGAG | 2 cut(s) | 302, 557 |
| HaeIII | GGCC | 4 cut(s) | 95, 222, 417, 477 |
| HapII | CCGG | 1 cut(s) | 36 |
| HgaI | GACGC | 1 cut(s) | 10 |
| Hin1II | CATG | 1 cut(s) | 54 |
| HinfI | GANTC | 5 cut(s) | 114, 133, 260, 369, 515 |
| HpaII | CCGG | 1 cut(s) | 36 |
| HphI | GGTGA | 4 cut(s) | 109, 236, 491, 679 |
| Hpy166II | GTNNAC | 3 cut(s) | 363, 618, 697 |
| Hpy188I | TCNGA | 2 cut(s) | 329, 584 |
| Hpy8I | GTNNAC | 3 cut(s) | 363, 618, 697 |
| HpyAV | CCTTC | 2 cut(s) | 347, 602 |
| HpyCH4III | ACNGT | 6 cut(s) | 88, 175, 188, 430, 443, 637 |
| HpyCH4V | TGCA | 1 cut(s) | 642 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 61 |
| HpyF3I | CTNAG | 1 cut(s) | 192 |
| Hsp92II | CATG | 1 cut(s) | 54 |
| KflI | GGGWCCC | 1 cut(s) | 387 |
| LguI | GCTCTTC | 2 cut(s) | 297, 552 |
| LmnI | GCTCC | 1 cut(s) | 69 |
| LpnPI | CCDG | 5 cut(s) | 49, 266, 293, 521, 548 |
| LweI | GCATC | 2 cut(s) | 165, 420 |
| MaeIII | GTNAC | 1 cut(s) | 22 |
| MboII | GAAGA | 7 cut(s) | 131, 284, 335, 386, 470, 539, 590 |
| MhlI | GDGCHC | 1 cut(s) | 219 |
| MlsI | TGGCCA | 1 cut(s) | 477 |
| MluCI | AATT | 1 cut(s) | 688 |
| MluNI | TGGCCA | 1 cut(s) | 477 |
| MlyI | GAGTC | 1 cut(s) | 127 |
| MmeI | TCCRAC | 1 cut(s) | 97 |
| MnlI | CCTC | 6 cut(s) | 43, 77, 80, 106, 160, 415 |
| Mox20I | TGGCCA | 1 cut(s) | 477 |
| MscI | TGGCCA | 1 cut(s) | 477 |
| MseI | TTAA | 3 cut(s) | 237, 492, 677 |
| Msp20I | TGGCCA | 1 cut(s) | 477 |
| MspI | CCGG | 1 cut(s) | 36 |
| MspR9I | CCNGG | 2 cut(s) | 281, 536 |
| MvaI | CCWGG | 2 cut(s) | 281, 536 |
| MvnI | CGCG | 1 cut(s) | 78 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 61 |
| NlaIII | CATG | 1 cut(s) | 54 |
| NlaIV | GGNNCC | 3 cut(s) | 31, 388, 389 |
| NmeAIII | GCCGAG | 2 cut(s) | 198, 682 |
| NmuCI | GTSAC | 1 cut(s) | 22 |
| PceI | AGGCCT | 1 cut(s) | 95 |
| PciSI | GCTCTTC | 2 cut(s) | 297, 552 |
| PfeI | GAWTC | 4 cut(s) | 114, 260, 369, 515 |
| PkrI | GCNGC | 1 cut(s) | 80 |
| PleI | GAGTC | 1 cut(s) | 127 |
| PpsI | GAGTC | 1 cut(s) | 127 |
| PpuMI | RGGWCCY | 1 cut(s) | 387 |
| Psp5II | RGGWCCY | 1 cut(s) | 387 |
| Psp6I | CCWGG | 2 cut(s) | 279, 534 |
| PspGI | CCWGG | 2 cut(s) | 279, 534 |
| PspN4I | GGNNCC | 3 cut(s) | 31, 388, 389 |
| PspPI | GGNCC | 1 cut(s) | 387 |
| PspPPI | RGGWCCY | 1 cut(s) | 387 |
| RsaI | GTAC | 4 cut(s) | 231, 274, 486, 529 |
| RsaNI | GTAC | 4 cut(s) | 230, 273, 485, 528 |
| SapI | GCTCTTC | 2 cut(s) | 297, 552 |
| SaqAI | TTAA | 3 cut(s) | 237, 492, 677 |
| SatI | GCNGC | 1 cut(s) | 79 |
| Sau96I | GGNCC | 1 cut(s) | 387 |
| ScaI | AGTACT | 2 cut(s) | 231, 486 |
| SchI | GAGTC | 1 cut(s) | 127 |
| ScrFI | CCNGG | 2 cut(s) | 281, 536 |
| SduI | GDGCHC | 1 cut(s) | 219 |
| SetI | ASST | 7 cut(s) | 15, 66, 152, 407, 634, 663, 702 |
| SfaNI | GCATC | 2 cut(s) | 165, 420 |
| SfcI | CTRYAG | 1 cut(s) | 633 |
| SinI | GGWCC | 1 cut(s) | 387 |
| Sse9I | AATT | 1 cut(s) | 688 |
| SseBI | AGGCCT | 1 cut(s) | 95 |
| SsiI | CCGC | 1 cut(s) | 78 |
| StuI | AGGCCT | 1 cut(s) | 95 |
| StyD4I | CCNGG | 2 cut(s) | 279, 534 |
| StyI | CCWWGG | 1 cut(s) | 447 |
| TaaI | ACNGT | 6 cut(s) | 88, 175, 188, 430, 443, 637 |
| TaqI | TCGA | 2 cut(s) | 351, 606 |
| TasI | AATT | 1 cut(s) | 688 |
| TatI | WGTACW | 2 cut(s) | 229, 484 |
| TauI | GCSGC | 1 cut(s) | 81 |
| TfiI | GAWTC | 4 cut(s) | 114, 260, 369, 515 |
| Tru1I | TTAA | 3 cut(s) | 237, 492, 677 |
| Tru9I | TTAA | 3 cut(s) | 237, 492, 677 |
| TscAI | CASTG | 1 cut(s) | 91 |
| TseFI | GTSAC | 1 cut(s) | 22 |
| Tsp45I | GTSAC | 1 cut(s) | 22 |
| TspDTI | ATGAA | 1 cut(s) | 39 |
| TspRI | CASTG | 1 cut(s) | 91 |
| VpaK11BI | GGWCC | 1 cut(s) | 387 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 351, 606 |
| XapI | RAATTY | 1 cut(s) | 688 |
| ZrmI | AGTACT | 2 cut(s) | 231, 486 |