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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 114 |
| AccI | GTMKAC | 2 cut(s) | 509, 732 |
| AciI | CCGC | 2 cut(s) | 344, 627 |
| AcoI | YGGCCR | 3 cut(s) | 138, 520, 591 |
| AcsI | RAATTY | 4 cut(s) | 93, 111, 396, 421 |
| AcuI | CTGAAG | 2 cut(s) | 606, 621 |
| AfaI | GTAC | 3 cut(s) | 104, 354, 698 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 114 |
| AflIII | ACRYGT | 1 cut(s) | 795 |
| AgsI | TTSAA | 9 cut(s) | 194, 245, 337, 464, 481, 538, 580, 721, 754 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 530, 562, 572, 604 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 628, 660 |
| AluBI | AGCT | 4 cut(s) | 241, 389, 599, 672 |
| AluI | AGCT | 4 cut(s) | 241, 389, 599, 672 |
| Alw26I | GTCTC | 3 cut(s) | 434, 446, 783 |
| AoxI | GGCC | 4 cut(s) | 116, 138, 520, 591 |
| ApoI | RAATTY | 4 cut(s) | 93, 111, 396, 421 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 771 |
| AspA2I | CCTAGG | 1 cut(s) | 119 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| AsuHPI | GGTGA | 1 cut(s) | 41 |
| AsuII | TTCGAA | 1 cut(s) | 634 |
| AvaII | GGWCC | 1 cut(s) | 157 |
| AvrII | CCTAGG | 1 cut(s) | 119 |
| BalI | TGGCCA | 2 cut(s) | 140, 522 |
| BbsI | GAAGAC | 1 cut(s) | 545 |
| BceAI | ACGGC | 1 cut(s) | 25 |
| BcgI | CGANNNNNNTGC | 3 cut(s) | 28, 255, 289 |
| BcoDI | GTCTC | 3 cut(s) | 434, 446, 783 |
| BfaI | CTAG | 4 cut(s) | 120, 209, 321, 663 |
| BglII | AGATCT | 1 cut(s) | 655 |
| BisI | GCNGC | 1 cut(s) | 627 |
| BlnI | CCTAGG | 1 cut(s) | 119 |
| BlsI | GCNGC | 1 cut(s) | 628 |
| Bme18I | GGWCC | 1 cut(s) | 157 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BmrI | ACTGGG | 1 cut(s) | 221 |
| BmsI | GCATC | 1 cut(s) | 723 |
| BmuI | ACTGGG | 1 cut(s) | 221 |
| BpiI | GAAGAC | 1 cut(s) | 545 |
| Bpu14I | TTCGAA | 1 cut(s) | 634 |
| BpuEI | CTTGAG | 1 cut(s) | 290 |
| BsaI | GGTCTC | 1 cut(s) | 783 |
| BsaJI | CCNNGG | 4 cut(s) | 119, 141, 315, 736 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 114 |
| Bse118I | RCCGGY | 1 cut(s) | 467 |
| Bse1I | ACTGG | 2 cut(s) | 227, 505 |
| BseDI | CCNNGG | 4 cut(s) | 119, 141, 315, 736 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 114 |
| BseMII | CTCAG | 2 cut(s) | 98, 392 |
| BseNI | ACTGG | 2 cut(s) | 227, 505 |
| Bsh1285I | CGRYCG | 1 cut(s) | 348 |
| BshFI | GGCC | 4 cut(s) | 118, 140, 522, 593 |
| BsiEI | CGRYCG | 1 cut(s) | 348 |
| BsiSI | CCGG | 1 cut(s) | 468 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 114 |
| BsmAI | GTCTC | 3 cut(s) | 434, 446, 783 |
| BsnI | GGCC | 4 cut(s) | 118, 140, 522, 593 |
| Bso31I | GGTCTC | 1 cut(s) | 783 |
| Bsp119I | TTCGAA | 1 cut(s) | 634 |
| Bsp143I | GATC | 3 cut(s) | 172, 514, 655 |
| BspACI | CCGC | 2 cut(s) | 344, 627 |
| BspANI | GGCC | 4 cut(s) | 118, 140, 522, 593 |
| BspCNI | CTCAG | 2 cut(s) | 97, 391 |
| BspT104I | TTCGAA | 1 cut(s) | 634 |
| BspTNI | GGTCTC | 1 cut(s) | 783 |
| BsrFI | RCCGGY | 1 cut(s) | 467 |
| BsrI | ACTGG | 2 cut(s) | 227, 505 |
| BssAI | RCCGGY | 1 cut(s) | 467 |
| BssECI | CCNNGG | 4 cut(s) | 119, 141, 315, 736 |
| BssMI | GATC | 3 cut(s) | 172, 514, 655 |
| BssT1I | CCWWGG | 3 cut(s) | 119, 141, 736 |
| Bst4CI | ACNGT | 1 cut(s) | 285 |
| Bst6I | CTCTTC | 2 cut(s) | 701, 786 |
| BstBI | TTCGAA | 1 cut(s) | 634 |
| BstC8I | GCNNGC | 1 cut(s) | 469 |
| BstDEI | CTNAG | 2 cut(s) | 84, 378 |
| BstKTI | GATC | 3 cut(s) | 175, 517, 658 |
| BstMAI | GTCTC | 3 cut(s) | 434, 446, 783 |
| BstMBI | GATC | 3 cut(s) | 172, 514, 655 |
| BstMCI | CGRYCG | 1 cut(s) | 348 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 623 |
| BstNSI | RCATGY | 1 cut(s) | 799 |
| BstV2I | GAAGAC | 1 cut(s) | 545 |
| BstX2I | RGATCY | 1 cut(s) | 655 |
| BstYI | RGATCY | 1 cut(s) | 655 |
| BsuRI | GGCC | 4 cut(s) | 118, 140, 522, 593 |
| Cac8I | GCNNGC | 1 cut(s) | 469 |
| Cfr10I | RCCGGY | 1 cut(s) | 467 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| Csp6I | GTAC | 3 cut(s) | 103, 353, 697 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 117, 152 |
| CviAII | CATG | 4 cut(s) | 20, 37, 401, 796 |
| CviQI | GTAC | 3 cut(s) | 103, 353, 697 |
| DdeI | CTNAG | 2 cut(s) | 84, 378 |
| DpnI | GATC | 3 cut(s) | 174, 516, 657 |
| DpnII | GATC | 3 cut(s) | 172, 514, 655 |
| EaeI | YGGCCR | 3 cut(s) | 138, 520, 591 |
| Eam1104I | CTCTTC | 2 cut(s) | 701, 786 |
| EarI | CTCTTC | 2 cut(s) | 701, 786 |
| Eco130I | CCWWGG | 3 cut(s) | 119, 141, 736 |
| Eco31I | GGTCTC | 1 cut(s) | 783 |
| Eco47I | GGWCC | 1 cut(s) | 157 |
| Eco57I | CTGAAG | 2 cut(s) | 606, 621 |
| EcoT14I | CCWWGG | 3 cut(s) | 119, 141, 736 |
| ErhI | CCWWGG | 3 cut(s) | 119, 141, 736 |
| FaeI | CATG | 4 cut(s) | 23, 40, 404, 799 |
| FatI | CATG | 4 cut(s) | 19, 36, 400, 795 |
| FblI | GTMKAC | 2 cut(s) | 509, 732 |
| Fnu4HI | GCNGC | 1 cut(s) | 627 |
| Fsp4HI | GCNGC | 1 cut(s) | 627 |
| FspBI | CTAG | 4 cut(s) | 120, 209, 321, 663 |
| GluI | GCNGC | 1 cut(s) | 627 |
| HaeIII | GGCC | 4 cut(s) | 118, 140, 522, 593 |
| HapII | CCGG | 1 cut(s) | 468 |
| Hin1II | CATG | 4 cut(s) | 23, 40, 404, 799 |
| HindIII | AAGCTT | 2 cut(s) | 239, 387 |
| HinfI | GANTC | 1 cut(s) | 272 |
| HpaII | CCGG | 1 cut(s) | 468 |
| HphI | GGTGA | 1 cut(s) | 41 |
| Hpy166II | GTNNAC | 4 cut(s) | 42, 128, 510, 733 |
| Hpy188I | TCNGA | 2 cut(s) | 281, 793 |
| Hpy188III | TCNNGA | 4 cut(s) | 72, 200, 443, 718 |
| Hpy8I | GTNNAC | 4 cut(s) | 42, 128, 510, 733 |
| HpyAV | CCTTC | 1 cut(s) | 256 |
| HpyCH4III | ACNGT | 1 cut(s) | 285 |
| HpyCH4IV | ACGT | 1 cut(s) | 76 |
| HpyCH4V | TGCA | 1 cut(s) | 745 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 623 |
| HpyF3I | CTNAG | 2 cut(s) | 84, 378 |
| HpySE526I | ACGT | 1 cut(s) | 76 |
| Hsp92II | CATG | 4 cut(s) | 23, 40, 404, 799 |
| KroI | GCCGGC | 1 cut(s) | 467 |
| KroNI | GCCGGC | 1 cut(s) | 469 |
| Kzo9I | GATC | 3 cut(s) | 172, 514, 655 |
| LpnPI | CCDG | 4 cut(s) | 240, 365, 481, 518 |
| LweI | GCATC | 1 cut(s) | 723 |
| MaeI | CTAG | 4 cut(s) | 120, 209, 321, 663 |
| MaeII | ACGT | 1 cut(s) | 76 |
| MaeIII | GTNAC | 2 cut(s) | 186, 310 |
| MalI | GATC | 3 cut(s) | 174, 516, 657 |
| MboI | GATC | 3 cut(s) | 172, 514, 655 |
| MboII | GAAGA | 6 cut(s) | 550, 592, 614, 718, 764, 773 |
| MflI | RGATCY | 1 cut(s) | 655 |
| MlsI | TGGCCA | 2 cut(s) | 140, 522 |
| MluCI | AATT | 5 cut(s) | 93, 111, 194, 396, 421 |
| MluNI | TGGCCA | 2 cut(s) | 140, 522 |
| MlyI | GAGTC | 1 cut(s) | 281 |
| MnlI | CCTC | 5 cut(s) | 310, 334, 547, 704, 799 |
| Mox20I | TGGCCA | 2 cut(s) | 140, 522 |
| MroNI | GCCGGC | 1 cut(s) | 467 |
| MroXI | GAANNNNTTC | 1 cut(s) | 771 |
| MscI | TGGCCA | 2 cut(s) | 140, 522 |
| MseI | TTAA | 1 cut(s) | 762 |
| Msp20I | TGGCCA | 2 cut(s) | 140, 522 |
| MspI | CCGG | 1 cut(s) | 468 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 623 |
| NaeI | GCCGGC | 1 cut(s) | 469 |
| NdeII | GATC | 3 cut(s) | 172, 514, 655 |
| NgoMIV | GCCGGC | 1 cut(s) | 467 |
| NlaIII | CATG | 4 cut(s) | 23, 40, 404, 799 |
| NspI | RCATGY | 1 cut(s) | 799 |
| NspV | TTCGAA | 1 cut(s) | 634 |
| PciI | ACATGT | 1 cut(s) | 795 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 73, 692 |
| PdiI | GCCGGC | 1 cut(s) | 469 |
| PdmI | GAANNNNTTC | 1 cut(s) | 771 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 114 |
| PkrI | GCNGC | 1 cut(s) | 628 |
| PleI | GAGTC | 1 cut(s) | 280 |
| PpsI | GAGTC | 1 cut(s) | 280 |
| PscI | ACATGT | 1 cut(s) | 795 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| PsuI | RGATCY | 1 cut(s) | 655 |
| RsaI | GTAC | 3 cut(s) | 104, 354, 698 |
| RsaNI | GTAC | 3 cut(s) | 103, 353, 697 |
| SaqAI | TTAA | 1 cut(s) | 762 |
| SatI | GCNGC | 1 cut(s) | 627 |
| Sau3AI | GATC | 3 cut(s) | 172, 514, 655 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| SchI | GAGTC | 1 cut(s) | 281 |
| SetI | ASST | 9 cut(s) | 79, 125, 243, 384, 391, 601, 674, 732, 742 |
| SfaNI | GCATC | 1 cut(s) | 723 |
| SfuI | TTCGAA | 1 cut(s) | 634 |
| SinI | GGWCC | 1 cut(s) | 157 |
| SmlI | CTYRAG | 1 cut(s) | 269 |
| SmoI | CTYRAG | 1 cut(s) | 269 |
| Sse9I | AATT | 5 cut(s) | 93, 111, 194, 396, 421 |
| SsiI | CCGC | 2 cut(s) | 344, 627 |
| SspI | AATATT | 1 cut(s) | 760 |
| SspMI | CTAG | 4 cut(s) | 120, 209, 321, 663 |
| StyI | CCWWGG | 3 cut(s) | 119, 141, 736 |
| TaaI | ACNGT | 1 cut(s) | 285 |
| TaiI | ACGT | 1 cut(s) | 79 |
| TaqI | TCGA | 4 cut(s) | 252, 348, 444, 634 |
| TaqII | GACCGA | 5 cut(s) | 22, 57, 265, 676, 765 |
| TasI | AATT | 5 cut(s) | 93, 111, 194, 396, 421 |
| TatI | WGTACW | 1 cut(s) | 352 |
| TauI | GCSGC | 1 cut(s) | 629 |
| Tru1I | TTAA | 1 cut(s) | 762 |
| Tru9I | TTAA | 1 cut(s) | 762 |
| TspDTI | ATGAA | 4 cut(s) | 8, 389, 408, 518 |
| TspGWI | ACGGA | 1 cut(s) | 395 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 114 |
| VpaK11BI | GGWCC | 1 cut(s) | 157 |
| XapI | RAATTY | 4 cut(s) | 93, 111, 396, 421 |
| XceI | RCATGY | 1 cut(s) | 799 |
| XmaJI | CCTAGG | 1 cut(s) | 119 |
| XmiI | GTMKAC | 2 cut(s) | 509, 732 |
| XmnI | GAANNNNTTC | 1 cut(s) | 771 |
| XspI | CTAG | 4 cut(s) | 120, 209, 321, 663 |