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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 446 |
| AccII | CGCG | 2 cut(s) | 90, 399 |
| AciI | CCGC | 5 cut(s) | 11, 360, 549, 1485, 1542 |
| AclWI | GGATC | 1 cut(s) | 1045 |
| AcoI | YGGCCR | 1 cut(s) | 157 |
| AcsI | RAATTY | 4 cut(s) | 245, 923, 1047, 1391 |
| AcuI | CTGAAG | 1 cut(s) | 1572 |
| AfaI | GTAC | 6 cut(s) | 453, 631, 1059, 1146, 1314, 1592 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 35 |
| AflII | CTTAAG | 1 cut(s) | 1508 |
| AgsI | TTSAA | 7 cut(s) | 292, 378, 466, 659, 849, 929, 1184 |
| AjnI | CCWGG | 2 cut(s) | 1414, 1605 |
| AluBI | AGCT | 6 cut(s) | 32, 554, 683, 934, 1066, 1096 |
| AluI | AGCT | 6 cut(s) | 32, 554, 683, 934, 1066, 1096 |
| Alw21I | GWGCWC | 1 cut(s) | 936 |
| Alw26I | GTCTC | 3 cut(s) | 959, 1486, 1641 |
| AlwI | GGATC | 1 cut(s) | 1045 |
| AoxI | GGCC | 3 cut(s) | 157, 284, 411 |
| ApeKI | GCWGC | 4 cut(s) | 176, 551, 1139, 1151 |
| ApoI | RAATTY | 4 cut(s) | 245, 923, 1047, 1391 |
| AspLEI | GCGC | 1 cut(s) | 399 |
| AspS9I | GGNCC | 3 cut(s) | 26, 1418, 1603 |
| AsuHPI | GGTGA | 7 cut(s) | 182, 184, 320, 514, 722, 745, 1364 |
| AvaII | GGWCC | 3 cut(s) | 26, 1418, 1603 |
| BanII | GRGCYC | 1 cut(s) | 936 |
| Bbv12I | GWGCWC | 1 cut(s) | 936 |
| BbvCI | CCTCAGC | 2 cut(s) | 363, 1488 |
| BbvI | GCAGC | 4 cut(s) | 163, 563, 1126, 1138 |
| BccI | CCATC | 3 cut(s) | 139, 203, 1512 |
| BceAI | ACGGC | 2 cut(s) | 144, 1127 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 28, 62, 68, 102, 1109, 1143, 1442, 1476 |
| BciT130I | CCWGG | 2 cut(s) | 1416, 1607 |
| BclI | TGATCA | 2 cut(s) | 109, 327 |
| BcoDI | GTCTC | 3 cut(s) | 959, 1486, 1641 |
| BfaI | CTAG | 2 cut(s) | 1044, 1293 |
| BfmI | CTRYAG | 1 cut(s) | 1152 |
| BfrI | CTTAAG | 1 cut(s) | 1508 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1148 |
| BisI | GCNGC | 7 cut(s) | 12, 177, 360, 549, 552, 1140, 1152 |
| BlsI | GCNGC | 7 cut(s) | 13, 178, 361, 550, 553, 1141, 1153 |
| Bme1390I | CCNGG | 2 cut(s) | 1416, 1607 |
| Bme18I | GGWCC | 3 cut(s) | 26, 1418, 1603 |
| BmgT120I | GGNCC | 3 cut(s) | 26, 1418, 1603 |
| BmiI | GGNNCC | 1 cut(s) | 27 |
| BmrFI | CCNGG | 2 cut(s) | 1416, 1607 |
| Bpu10I | CCTNAGC | 2 cut(s) | 363, 1488 |
| Bsa29I | ATCGAT | 2 cut(s) | 42, 1458 |
| BsaBI | GATNNNNATC | 3 cut(s) | 326, 699, 1523 |
| BsaJI | CCNNGG | 2 cut(s) | 1053, 1219 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 887, 917 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 35 |
| Bse3DI | GCAATG | 1 cut(s) | 790 |
| Bse8I | GATNNNNATC | 3 cut(s) | 326, 699, 1523 |
| BseBI | CCWGG | 2 cut(s) | 1416, 1607 |
| BseCI | ATCGAT | 2 cut(s) | 42, 1458 |
| BseDI | CCNNGG | 2 cut(s) | 1053, 1219 |
| BseGI | GGATG | 1 cut(s) | 1207 |
| BseJI | GATNNNNATC | 3 cut(s) | 326, 699, 1523 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 35 |
| BseMI | GCAATG | 1 cut(s) | 790 |
| BseMII | CTCAG | 4 cut(s) | 354, 400, 488, 1479 |
| BseRI | GAGGAG | 1 cut(s) | 380 |
| BseXI | GCAGC | 4 cut(s) | 163, 563, 1126, 1138 |
| BseYI | CCCAGC | 1 cut(s) | 1384 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 399 |
| Bsh1285I | CGRYCG | 1 cut(s) | 88 |
| BshFI | GGCC | 3 cut(s) | 159, 286, 413 |
| BshVI | ATCGAT | 2 cut(s) | 42, 1458 |
| BsiEI | CGRYCG | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 1 cut(s) | 936 |
| BsiSI | CCGG | 1 cut(s) | 690 |
| BslFI | GGGAC | 1 cut(s) | 1244 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 35 |
| BsmAI | GTCTC | 3 cut(s) | 959, 1486, 1641 |
| BsmFI | GGGAC | 1 cut(s) | 1244 |
| BsnI | GGCC | 3 cut(s) | 159, 286, 413 |
| Bsp1286I | GDGCHC | 1 cut(s) | 936 |
| BspACI | CCGC | 5 cut(s) | 11, 360, 549, 1485, 1542 |
| BspANI | GGCC | 3 cut(s) | 159, 286, 413 |
| BspCNI | CTCAG | 4 cut(s) | 355, 399, 487, 1480 |
| BspDI | ATCGAT | 2 cut(s) | 42, 1458 |
| BspFNI | CGCG | 2 cut(s) | 90, 399 |
| BspLI | GGNNCC | 1 cut(s) | 27 |
| BspMAI | CTGCAG | 1 cut(s) | 1156 |
| BspPI | GGATC | 1 cut(s) | 1045 |
| BspQI | GCTCTTC | 1 cut(s) | 924 |
| BspTI | CTTAAG | 1 cut(s) | 1508 |
| BsrDI | GCAATG | 1 cut(s) | 790 |
| BssECI | CCNNGG | 2 cut(s) | 1053, 1219 |
| BssT1I | CCWWGG | 2 cut(s) | 1053, 1219 |
| Bst2UI | CCWGG | 2 cut(s) | 1416, 1607 |
| Bst4CI | ACNGT | 5 cut(s) | 214, 451, 970, 1136, 1309 |
| Bst6I | CTCTTC | 1 cut(s) | 924 |
| BstAFI | CTTAAG | 1 cut(s) | 1508 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 557, 1136 |
| BstDEI | CTNAG | 6 cut(s) | 363, 386, 474, 800, 1488, 1632 |
| BstF5I | GGATG | 1 cut(s) | 1207 |
| BstFNI | CGCG | 2 cut(s) | 90, 399 |
| BstHHI | GCGC | 1 cut(s) | 399 |
| BstMAI | GTCTC | 3 cut(s) | 959, 1486, 1641 |
| BstMCI | CGRYCG | 1 cut(s) | 88 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 940, 1136, 1148, 1637 |
| BstNI | CCWGG | 2 cut(s) | 1416, 1607 |
| BstNSI | RCATGY | 2 cut(s) | 261, 943 |
| BstSCI | CCNGG | 2 cut(s) | 1414, 1605 |
| BstSFI | CTRYAG | 1 cut(s) | 1152 |
| BstUI | CGCG | 2 cut(s) | 90, 399 |
| BstV1I | GCAGC | 4 cut(s) | 163, 563, 1126, 1138 |
| BstX2I | RGATCY | 1 cut(s) | 1037 |
| BstYI | RGATCY | 1 cut(s) | 1037 |
| Bsu15I | ATCGAT | 2 cut(s) | 42, 1458 |
| BsuRI | GGCC | 3 cut(s) | 159, 286, 413 |
| BsuTUI | ATCGAT | 2 cut(s) | 42, 1458 |
| BtsCI | GGATG | 1 cut(s) | 1207 |
| BtsIMutI | CAGTG | 1 cut(s) | 210 |
| CfoI | GCGC | 1 cut(s) | 399 |
| Cfr13I | GGNCC | 3 cut(s) | 26, 1418, 1603 |
| ClaI | ATCGAT | 2 cut(s) | 42, 1458 |
| Csp6I | GTAC | 6 cut(s) | 452, 630, 1058, 1145, 1313, 1591 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 280, 315, 571, 606 |
| CviAII | CATG | 5 cut(s) | 63, 258, 514, 940, 1163 |
| CviQI | GTAC | 6 cut(s) | 452, 630, 1058, 1145, 1313, 1591 |
| DdeI | CTNAG | 6 cut(s) | 363, 386, 474, 800, 1488, 1632 |
| EaeI | YGGCCR | 1 cut(s) | 157 |
| Eam1104I | CTCTTC | 1 cut(s) | 924 |
| EarI | CTCTTC | 1 cut(s) | 924 |
| Ecl136II | GAGCTC | 1 cut(s) | 934 |
| Eco130I | CCWWGG | 2 cut(s) | 1053, 1219 |
| Eco147I | AGGCCT | 1 cut(s) | 413 |
| Eco24I | GRGCYC | 1 cut(s) | 936 |
| Eco32I | GATATC | 1 cut(s) | 372 |
| Eco47I | GGWCC | 3 cut(s) | 26, 1418, 1603 |
| Eco53kI | GAGCTC | 1 cut(s) | 934 |
| Eco57I | CTGAAG | 1 cut(s) | 1572 |
| EcoICRI | GAGCTC | 1 cut(s) | 934 |
| EcoRI | GAATTC | 1 cut(s) | 1047 |
| EcoRII | CCWGG | 2 cut(s) | 1414, 1605 |
| EcoRV | GATATC | 1 cut(s) | 372 |
| EcoT14I | CCWWGG | 2 cut(s) | 1053, 1219 |
| EcoT22I | ATGCAT | 2 cut(s) | 259, 945 |
| EcoT38I | GRGCYC | 1 cut(s) | 936 |
| ErhI | CCWWGG | 2 cut(s) | 1053, 1219 |
| FaeI | CATG | 5 cut(s) | 66, 261, 517, 943, 1166 |
| FaqI | GGGAC | 1 cut(s) | 1244 |
| FatI | CATG | 5 cut(s) | 62, 257, 513, 939, 1162 |
| FauI | CCCGC | 1 cut(s) | 1535 |
| FauNDI | CATATG | 1 cut(s) | 562 |
| FbaI | TGATCA | 2 cut(s) | 109, 327 |
| Fnu4HI | GCNGC | 7 cut(s) | 12, 177, 360, 549, 552, 1140, 1152 |
| FokI | GGATG | 1 cut(s) | 1214 |
| FriOI | GRGCYC | 1 cut(s) | 936 |
| Fsp4HI | GCNGC | 7 cut(s) | 12, 177, 360, 549, 552, 1140, 1152 |
| FspBI | CTAG | 2 cut(s) | 1044, 1293 |
| GlaI | GCGC | 1 cut(s) | 398 |
| GluI | GCNGC | 7 cut(s) | 12, 177, 360, 549, 552, 1140, 1152 |
| GsaI | CCCAGC | 1 cut(s) | 1388 |
| HaeIII | GGCC | 3 cut(s) | 159, 286, 413 |
| HapII | CCGG | 1 cut(s) | 690 |
| HhaI | GCGC | 1 cut(s) | 399 |
| Hin1II | CATG | 5 cut(s) | 66, 261, 517, 943, 1166 |
| Hin6I | GCGC | 1 cut(s) | 397 |
| HinP1I | GCGC | 1 cut(s) | 397 |
| HinfI | GANTC | 5 cut(s) | 470, 838, 875, 1407, 1500 |
| HpaII | CCGG | 1 cut(s) | 690 |
| HphI | GGTGA | 7 cut(s) | 182, 184, 320, 514, 722, 745, 1364 |
| Hpy166II | GTNNAC | 1 cut(s) | 736 |
| Hpy188I | TCNGA | 2 cut(s) | 50, 1552 |
| Hpy188III | TCNNGA | 5 cut(s) | 569, 757, 1115, 1184, 1453 |
| Hpy8I | GTNNAC | 1 cut(s) | 736 |
| HpyAV | CCTTC | 5 cut(s) | 656, 766, 1171, 1570, 1621 |
| HpyCH4III | ACNGT | 5 cut(s) | 214, 451, 970, 1136, 1309 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 940, 1136, 1148, 1637 |
| HpyF3I | CTNAG | 6 cut(s) | 363, 386, 474, 800, 1488, 1632 |
| Hsp92II | CATG | 5 cut(s) | 66, 261, 517, 943, 1166 |
| HspAI | GCGC | 1 cut(s) | 397 |
| Ksp22I | TGATCA | 2 cut(s) | 109, 327 |
| LguI | GCTCTTC | 1 cut(s) | 924 |
| LmnI | GCTCC | 1 cut(s) | 1093 |
| Lsp1109I | GCAGC | 4 cut(s) | 163, 563, 1126, 1138 |
| MaeI | CTAG | 2 cut(s) | 1044, 1293 |
| MaeIII | GTNAC | 4 cut(s) | 190, 308, 1204, 1232 |
| MboII | GAAGA | 9 cut(s) | 366, 671, 725, 772, 816, 837, 941, 1367, 1579 |
| MflI | RGATCY | 1 cut(s) | 1037 |
| MhlI | GDGCHC | 1 cut(s) | 936 |
| MlyI | GAGTC | 3 cut(s) | 464, 832, 1494 |
| MmeI | TCCRAC | 2 cut(s) | 49, 1179 |
| Mph1103I | ATGCAT | 2 cut(s) | 259, 945 |
| MseI | TTAA | 5 cut(s) | 264, 534, 651, 1083, 1509 |
| MslI | CAYNNNNRTG | 1 cut(s) | 518 |
| MspA1I | CMGCKG | 2 cut(s) | 362, 1487 |
| MspCI | CTTAAG | 1 cut(s) | 1508 |
| MspI | CCGG | 1 cut(s) | 690 |
| MspR9I | CCNGG | 2 cut(s) | 1416, 1607 |
| MvaI | CCWGG | 2 cut(s) | 1416, 1607 |
| MvnI | CGCG | 2 cut(s) | 90, 399 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 940, 1136, 1148, 1637 |
| NdeI | CATATG | 1 cut(s) | 562 |
| NlaIII | CATG | 5 cut(s) | 66, 261, 517, 943, 1166 |
| NlaIV | GGNNCC | 1 cut(s) | 27 |
| NmuCI | GTSAC | 2 cut(s) | 190, 308 |
| NsiI | ATGCAT | 2 cut(s) | 259, 945 |
| NspI | RCATGY | 2 cut(s) | 261, 943 |
| PceI | AGGCCT | 1 cut(s) | 413 |
| PciSI | GCTCTTC | 1 cut(s) | 924 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 131, 891, 1140 |
| PfeI | GAWTC | 2 cut(s) | 875, 1407 |
| PkrI | GCNGC | 7 cut(s) | 13, 178, 361, 550, 553, 1141, 1153 |
| Ple19I | CGATCG | 1 cut(s) | 88 |
| PleI | GAGTC | 3 cut(s) | 464, 832, 1494 |
| PpsI | GAGTC | 3 cut(s) | 464, 832, 1494 |
| PsiI | TTATAA | 1 cut(s) | 446 |
| Psp124BI | GAGCTC | 1 cut(s) | 936 |
| Psp6I | CCWGG | 2 cut(s) | 1414, 1605 |
| PspFI | CCCAGC | 1 cut(s) | 1384 |
| PspGI | CCWGG | 2 cut(s) | 1414, 1605 |
| PspN4I | GGNNCC | 1 cut(s) | 27 |
| PspPI | GGNCC | 3 cut(s) | 26, 1418, 1603 |
| PstI | CTGCAG | 1 cut(s) | 1156 |
| PsuI | RGATCY | 1 cut(s) | 1037 |
| PvuI | CGATCG | 1 cut(s) | 88 |
| RsaI | GTAC | 6 cut(s) | 453, 631, 1059, 1146, 1314, 1592 |
| RsaNI | GTAC | 6 cut(s) | 452, 630, 1058, 1145, 1313, 1591 |
| RseI | CAYNNNNRTG | 1 cut(s) | 518 |
| SacI | GAGCTC | 1 cut(s) | 936 |
| SapI | GCTCTTC | 1 cut(s) | 924 |
| SaqAI | TTAA | 5 cut(s) | 264, 534, 651, 1083, 1509 |
| SatI | GCNGC | 7 cut(s) | 12, 177, 360, 549, 552, 1140, 1152 |
| Sau96I | GGNCC | 3 cut(s) | 26, 1418, 1603 |
| SchI | GAGTC | 3 cut(s) | 464, 832, 1494 |
| ScrFI | CCNGG | 2 cut(s) | 1416, 1607 |
| SduI | GDGCHC | 1 cut(s) | 936 |
| SfcI | CTRYAG | 1 cut(s) | 1152 |
| SinI | GGWCC | 3 cut(s) | 26, 1418, 1603 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 518 |
| SmlI | CTYRAG | 1 cut(s) | 1508 |
| SmoI | CTYRAG | 1 cut(s) | 1508 |
| SseBI | AGGCCT | 1 cut(s) | 413 |
| SsiI | CCGC | 5 cut(s) | 11, 360, 549, 1485, 1542 |
| SspI | AATATT | 1 cut(s) | 154 |
| SspMI | CTAG | 2 cut(s) | 1044, 1293 |
| SstI | GAGCTC | 1 cut(s) | 936 |
| StuI | AGGCCT | 1 cut(s) | 413 |
| StyD4I | CCNGG | 2 cut(s) | 1414, 1605 |
| StyI | CCWWGG | 2 cut(s) | 1053, 1219 |
| TaaI | ACNGT | 5 cut(s) | 214, 451, 970, 1136, 1309 |
| TaqI | TCGA | 7 cut(s) | 42, 615, 756, 885, 894, 1452, 1458 |
| TatI | WGTACW | 1 cut(s) | 1590 |
| TauI | GCSGC | 3 cut(s) | 14, 362, 551 |
| TfiI | GAWTC | 2 cut(s) | 875, 1407 |
| Tru1I | TTAA | 5 cut(s) | 264, 534, 651, 1083, 1509 |
| Tru9I | TTAA | 5 cut(s) | 264, 534, 651, 1083, 1509 |
| TscAI | CASTG | 1 cut(s) | 217 |
| TseFI | GTSAC | 2 cut(s) | 190, 308 |
| TseI | GCWGC | 4 cut(s) | 176, 551, 1139, 1151 |
| Tsp45I | GTSAC | 2 cut(s) | 190, 308 |
| TspDTI | ATGAA | 5 cut(s) | 51, 290, 357, 530, 1589 |
| TspRI | CASTG | 1 cut(s) | 217 |
| Vha464I | CTTAAG | 1 cut(s) | 1508 |
| VpaK11BI | GGWCC | 3 cut(s) | 26, 1418, 1603 |
| XapI | RAATTY | 4 cut(s) | 245, 923, 1047, 1391 |
| XceI | RCATGY | 2 cut(s) | 261, 943 |
| XspI | CTAG | 2 cut(s) | 1044, 1293 |
| Zsp2I | ATGCAT | 2 cut(s) | 259, 945 |