dipeptidyl-peptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 763 |
| AccB1I | GGYRCC | 1 cut(s) | 1818 |
| AccI | GTMKAC | 2 cut(s) | 1933, 2121 |
| AccII | CGCG | 1 cut(s) | 288 |
| AciI | CCGC | 3 cut(s) | 12, 1045, 2075 |
| AclI | AACGTT | 1 cut(s) | 1247 |
| AclWI | GGATC | 3 cut(s) | 1714, 2140, 2322 |
| AcoI | YGGCCR | 5 cut(s) | 3, 917, 1644, 1704, 1915 |
| AcsI | RAATTY | 4 cut(s) | 86, 1065, 1123, 1285 |
| AcuI | CTGAAG | 4 cut(s) | 380, 665, 765, 2055 |
| AcyI | GRCGYC | 1 cut(s) | 402 |
| AfaI | GTAC | 6 cut(s) | 480, 954, 1115, 1482, 1973, 1993 |
| AfeI | AGCGCT | 1 cut(s) | 2172 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 597, 899, 1815, 2136 |
| AflII | CTTAAG | 2 cut(s) | 565, 1838 |
| AflIII | ACRYGT | 4 cut(s) | 331, 1178, 2116, 2324 |
| AjiI | CACGTC | 2 cut(s) | 447, 1136 |
| AjnI | CCWGG | 2 cut(s) | 1615, 2058 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 320, 352 |
| AluBI | AGCT | 6 cut(s) | 110, 251, 259, 1330, 1654, 2070 |
| AluI | AGCT | 6 cut(s) | 110, 251, 259, 1330, 1654, 2070 |
| Alw21I | GWGCWC | 3 cut(s) | 654, 1703, 2376 |
| Alw26I | GTCTC | 3 cut(s) | 28, 587, 1919 |
| AlwI | GGATC | 3 cut(s) | 1714, 2140, 2322 |
| Aor51HI | AGCGCT | 1 cut(s) | 2172 |
| ApaI | GGGCCC | 1 cut(s) | 1014 |
| ApeKI | GCWGC | 1 cut(s) | 1682 |
| ApoI | RAATTY | 4 cut(s) | 86, 1065, 1123, 1285 |
| AspLEI | GCGC | 7 cut(s) | 288, 764, 1095, 1406, 1422, 2065, 2173 |
| AspS9I | GGNCC | 6 cut(s) | 941, 1010, 1011, 1733, 1742, 2362 |
| AsuC2I | CCSGG | 1 cut(s) | 1214 |
| AsuHPI | GGTGA | 6 cut(s) | 18, 27, 431, 1486, 1648, 2181 |
| AvaII | GGWCC | 1 cut(s) | 941 |
| BaeGI | GKGCMC | 2 cut(s) | 1014, 1823 |
| BalI | TGGCCA | 2 cut(s) | 5, 919 |
| BanI | GGYRCC | 1 cut(s) | 1818 |
| BanII | GRGCYC | 1 cut(s) | 1014 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 945, 977 |
| BbsI | GAAGAC | 3 cut(s) | 368, 1033, 2092 |
| Bbv12I | GWGCWC | 3 cut(s) | 654, 1703, 2376 |
| BbvI | GCAGC | 1 cut(s) | 1694 |
| BccI | CCATC | 7 cut(s) | 37, 497, 977, 1415, 1499, 1754, 2384 |
| BceAI | ACGGC | 3 cut(s) | 689, 888, 2101 |
| BciT130I | CCWGG | 2 cut(s) | 1617, 2060 |
| BcnI | CCSGG | 1 cut(s) | 1214 |
| BcoDI | GTCTC | 3 cut(s) | 28, 587, 1919 |
| BfaI | CTAG | 4 cut(s) | 956, 1049, 1799, 2046 |
| BfoI | RGCGCY | 3 cut(s) | 1407, 2066, 2174 |
| BfrI | CTTAAG | 2 cut(s) | 565, 1838 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 232, 1740 |
| BisI | GCNGC | 1 cut(s) | 1683 |
| BlpI | GCTNAGC | 2 cut(s) | 2066, 2071 |
| BlsI | GCNGC | 1 cut(s) | 1684 |
| BmcAI | AGTACT | 1 cut(s) | 480 |
| Bme1390I | CCNGG | 3 cut(s) | 1214, 1617, 2060 |
| Bme18I | GGWCC | 1 cut(s) | 941 |
| BmgBI | CACGTC | 2 cut(s) | 447, 1136 |
| BmgT120I | GGNCC | 6 cut(s) | 941, 1010, 1011, 1733, 1742, 2362 |
| BmiI | GGNNCC | 5 cut(s) | 943, 1012, 1744, 1820, 2363 |
| BmrFI | CCNGG | 3 cut(s) | 1214, 1617, 2060 |
| BmsI | GCATC | 9 cut(s) | 793, 1095, 1199, 1465, 1521, 1618, 1629, 1800, 2331 |
| BoxI | GACNNNNGTC | 1 cut(s) | 316 |
| BpiI | GAAGAC | 3 cut(s) | 368, 1033, 2092 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2133, 2165 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2385 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2066, 2071 |
| BpuEI | CTTGAG | 1 cut(s) | 373 |
| BpuMI | CCSGG | 1 cut(s) | 1214 |
| BsaAI | YACGTR | 1 cut(s) | 1181 |
| BsaBI | GATNNNNATC | 1 cut(s) | 495 |
| BsaHI | GRCGYC | 1 cut(s) | 402 |
| BsaJI | CCNNGG | 5 cut(s) | 655, 1571, 1659, 1918, 2058 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 597, 899, 1815, 2136 |
| Bse118I | RCCGGY | 2 cut(s) | 712, 2026 |
| Bse1I | ACTGG | 4 cut(s) | 610, 1973, 2046, 2158 |
| Bse3DI | GCAATG | 3 cut(s) | 930, 1018, 1170 |
| Bse8I | GATNNNNATC | 1 cut(s) | 495 |
| BseBI | CCWGG | 2 cut(s) | 1617, 2060 |
| BseDI | CCNNGG | 5 cut(s) | 655, 1571, 1659, 1918, 2058 |
| BseGI | GGATG | 7 cut(s) | 969, 1190, 1480, 1749, 1791, 2346, 2395 |
| BseJI | GATNNNNATC | 1 cut(s) | 495 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 597, 899, 1815, 2136 |
| BseMI | GCAATG | 3 cut(s) | 930, 1018, 1170 |
| BseMII | CTCAG | 3 cut(s) | 1026, 1776, 2085 |
| BseNI | ACTGG | 4 cut(s) | 610, 1973, 2046, 2158 |
| BseRI | GAGGAG | 2 cut(s) | 574, 2196 |
| BseSI | GKGCMC | 2 cut(s) | 1014, 1823 |
| BseXI | GCAGC | 1 cut(s) | 1694 |
| Bsh1236I | CGCG | 1 cut(s) | 288 |
| Bsh1285I | CGRYCG | 4 cut(s) | 320, 847, 1112, 1218 |
| BshNI | GGYRCC | 1 cut(s) | 1818 |
| BsiEI | CGRYCG | 4 cut(s) | 320, 847, 1112, 1218 |
| BsiHKAI | GWGCWC | 3 cut(s) | 654, 1703, 2376 |
| BsiSI | CCGG | 3 cut(s) | 713, 1214, 2027 |
| BsiWI | CGTACG | 1 cut(s) | 1113 |
| BslFI | GGGAC | 1 cut(s) | 1569 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 597, 899, 1815, 2136 |
| BsmAI | GTCTC | 3 cut(s) | 28, 587, 1919 |
| BsmBI | CGTCTC | 1 cut(s) | 1919 |
| BsmFI | GGGAC | 1 cut(s) | 1569 |
| BsmI | GAATGC | 2 cut(s) | 806, 1273 |
| Bsp120I | GGGCCC | 1 cut(s) | 1010 |
| Bsp1286I | GDGCHC | 5 cut(s) | 654, 1014, 1703, 1823, 2376 |
| Bsp143I | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2066, 2071 |
| Bsp19I | CCATGG | 1 cut(s) | 1659 |
| BspACI | CCGC | 3 cut(s) | 12, 1045, 2075 |
| BspCNI | CTCAG | 3 cut(s) | 1027, 1775, 2084 |
| BspFNI | CGCG | 1 cut(s) | 288 |
| BspHI | TCATGA | 2 cut(s) | 1455, 2209 |
| BspLI | GGNNCC | 5 cut(s) | 943, 1012, 1744, 1820, 2363 |
| BspPI | GGATC | 3 cut(s) | 1714, 2140, 2322 |
| BspQI | GCTCTTC | 1 cut(s) | 1508 |
| BspT107I | GGYRCC | 1 cut(s) | 1818 |
| BspTI | CTTAAG | 2 cut(s) | 565, 1838 |
| BsrDI | GCAATG | 3 cut(s) | 930, 1018, 1170 |
| BsrFI | RCCGGY | 2 cut(s) | 712, 2026 |
| BsrI | ACTGG | 4 cut(s) | 610, 1973, 2046, 2158 |
| BssAI | RCCGGY | 2 cut(s) | 712, 2026 |
| BssECI | CCNNGG | 5 cut(s) | 655, 1571, 1659, 1918, 2058 |
| BssMI | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| BssNI | GRCGYC | 1 cut(s) | 402 |
| BssT1I | CCWWGG | 3 cut(s) | 655, 1571, 1659 |
| Bst2UI | CCWGG | 2 cut(s) | 1617, 2060 |
| Bst4CI | ACNGT | 4 cut(s) | 64, 318, 419, 727 |
| Bst6I | CTCTTC | 3 cut(s) | 1160, 1472, 1508 |
| BstACI | GRCGYC | 1 cut(s) | 402 |
| BstAFI | CTTAAG | 2 cut(s) | 565, 1838 |
| BstBAI | YACGTR | 1 cut(s) | 1181 |
| BstC8I | GCNNGC | 4 cut(s) | 237, 241, 371, 1915 |
| BstDEI | CTNAG | 6 cut(s) | 1035, 1688, 1762, 2066, 2071, 2385 |
| BstDSI | CCRYGG | 1 cut(s) | 1659 |
| BstEII | GGTNACC | 1 cut(s) | 605 |
| BstF5I | GGATG | 7 cut(s) | 969, 1190, 1480, 1749, 1791, 2346, 2395 |
| BstFNI | CGCG | 1 cut(s) | 288 |
| BstH2I | RGCGCY | 3 cut(s) | 1407, 2066, 2174 |
| BstHHI | GCGC | 7 cut(s) | 288, 764, 1095, 1406, 1422, 2065, 2173 |
| BstKTI | GATC | 8 cut(s) | 306, 847, 1112, 1120, 1722, 2016, 2148, 2317 |
| BstMAI | GTCTC | 3 cut(s) | 28, 587, 1919 |
| BstMBI | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| BstMCI | CGRYCG | 4 cut(s) | 320, 847, 1112, 1218 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 11, 232, 285, 379, 1092, 1698, 1740 |
| BstNI | CCWGG | 2 cut(s) | 1617, 2060 |
| BstNSI | RCATGY | 4 cut(s) | 239, 335, 2120, 2328 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 316 |
| BstPI | GGTNACC | 1 cut(s) | 605 |
| BstSCI | CCNGG | 3 cut(s) | 1212, 1615, 2058 |
| BstSLI | GKGCMC | 2 cut(s) | 1014, 1823 |
| BstUI | CGCG | 1 cut(s) | 288 |
| BstV1I | GCAGC | 1 cut(s) | 1694 |
| BstV2I | GAAGAC | 3 cut(s) | 368, 1033, 2092 |
| BstX2I | RGATCY | 1 cut(s) | 2145 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1913 |
| BstYI | RGATCY | 1 cut(s) | 2145 |
| BtgI | CCRYGG | 1 cut(s) | 1659 |
| BtrI | CACGTC | 2 cut(s) | 447, 1136 |
| BtsCI | GGATG | 7 cut(s) | 969, 1190, 1480, 1749, 1791, 2346, 2395 |
| BtsI | GCAGTG | 1 cut(s) | 632 |
| BtsIMutI | CAGTG | 2 cut(s) | 632, 2039 |
| Cac8I | GCNNGC | 4 cut(s) | 237, 241, 371, 1915 |
| CciI | TCATGA | 2 cut(s) | 1455, 2209 |
| CfoI | GCGC | 7 cut(s) | 288, 764, 1095, 1406, 1422, 2065, 2173 |
| Cfr10I | RCCGGY | 2 cut(s) | 712, 2026 |
| Cfr13I | GGNCC | 6 cut(s) | 941, 1010, 1011, 1733, 1742, 2362 |
| CseI | GACGC | 2 cut(s) | 391, 631 |
| Csp6I | GTAC | 6 cut(s) | 479, 953, 1114, 1481, 1972, 1992 |
| CviQI | GTAC | 6 cut(s) | 479, 953, 1114, 1481, 1972, 1992 |
| DdeI | CTNAG | 6 cut(s) | 1035, 1688, 1762, 2066, 2071, 2385 |
| DpnI | GATC | 8 cut(s) | 305, 846, 1111, 1119, 1721, 2015, 2147, 2316 |
| DpnII | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| DraI | TTTAAA | 2 cut(s) | 1252, 1335 |
| EaeI | YGGCCR | 5 cut(s) | 3, 917, 1644, 1704, 1915 |
| Eam1104I | CTCTTC | 3 cut(s) | 1160, 1472, 1508 |
| EarI | CTCTTC | 3 cut(s) | 1160, 1472, 1508 |
| EciI | GGCGGA | 1 cut(s) | 1034 |
| Eco130I | CCWWGG | 3 cut(s) | 655, 1571, 1659 |
| Eco24I | GRGCYC | 1 cut(s) | 1014 |
| Eco47I | GGWCC | 1 cut(s) | 941 |
| Eco47III | AGCGCT | 1 cut(s) | 2172 |
| Eco57I | CTGAAG | 4 cut(s) | 380, 665, 765, 2055 |
| Eco91I | GGTNACC | 1 cut(s) | 605 |
| EcoO109I | RGGNCCY | 2 cut(s) | 941, 2362 |
| EcoO65I | GGTNACC | 1 cut(s) | 605 |
| EcoRI | GAATTC | 1 cut(s) | 1123 |
| EcoRII | CCWGG | 2 cut(s) | 1615, 2058 |
| EcoT14I | CCWWGG | 3 cut(s) | 655, 1571, 1659 |
| EcoT22I | ATGCAT | 3 cut(s) | 808, 1088, 2346 |
| EcoT38I | GRGCYC | 1 cut(s) | 1014 |
| ErhI | CCWWGG | 3 cut(s) | 655, 1571, 1659 |
| Esp3I | CGTCTC | 1 cut(s) | 1919 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1659, 1691, 1865, 1897 |
| FaqI | GGGAC | 1 cut(s) | 1569 |
| FblI | GTMKAC | 2 cut(s) | 1933, 2121 |
| Fnu4HI | GCNGC | 1 cut(s) | 1683 |
| FokI | GGATG | 6 cut(s) | 956, 1177, 1487, 1736, 1778, 2353 |
| FriOI | GRGCYC | 1 cut(s) | 1014 |
| Fsp4HI | GCNGC | 1 cut(s) | 1683 |
| FspBI | CTAG | 4 cut(s) | 956, 1049, 1799, 2046 |
| FspI | TGCGCA | 1 cut(s) | 763 |
| GlaI | GCGC | 7 cut(s) | 287, 763, 1094, 1405, 1421, 2064, 2172 |
| GluI | GCNGC | 1 cut(s) | 1683 |
| HaeII | RGCGCY | 3 cut(s) | 1407, 2066, 2174 |
| HapII | CCGG | 3 cut(s) | 713, 1214, 2027 |
| HgaI | GACGC | 2 cut(s) | 391, 631 |
| HhaI | GCGC | 7 cut(s) | 288, 764, 1095, 1406, 1422, 2065, 2173 |
| Hin1I | GRCGYC | 1 cut(s) | 402 |
| Hin6I | GCGC | 7 cut(s) | 286, 762, 1093, 1404, 1420, 2063, 2171 |
| HinP1I | GCGC | 7 cut(s) | 286, 762, 1093, 1404, 1420, 2063, 2171 |
| HincII | GTYRAC | 1 cut(s) | 207 |
| HindII | GTYRAC | 1 cut(s) | 207 |
| HindIII | AAGCTT | 1 cut(s) | 1328 |
| HinfI | GANTC | 8 cut(s) | 393, 456, 924, 1220, 1638, 1768, 1828, 1930 |
| HpaII | CCGG | 3 cut(s) | 713, 1214, 2027 |
| HphI | GGTGA | 6 cut(s) | 18, 27, 431, 1486, 1648, 2181 |
| Hpy166II | GTNNAC | 7 cut(s) | 55, 80, 207, 389, 1934, 2122, 2163 |
| Hpy188I | TCNGA | 2 cut(s) | 1036, 1122 |
| Hpy8I | GTNNAC | 7 cut(s) | 55, 80, 207, 389, 1934, 2122, 2163 |
| Hpy99I | CGWCG | 4 cut(s) | 401, 407, 625, 1828 |
| HpyCH4III | ACNGT | 4 cut(s) | 64, 318, 419, 727 |
| HpyCH4IV | ACGT | 6 cut(s) | 266, 446, 1135, 1180, 1247, 2260 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 11, 232, 285, 379, 1092, 1698, 1740 |
| HpyF3I | CTNAG | 6 cut(s) | 1035, 1688, 1762, 2066, 2071, 2385 |
| HpySE526I | ACGT | 6 cut(s) | 266, 446, 1135, 1180, 1247, 2260 |
| Hsp92I | GRCGYC | 1 cut(s) | 402 |
| HspAI | GCGC | 7 cut(s) | 286, 762, 1093, 1404, 1420, 2063, 2171 |
| Kzo9I | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| LguI | GCTCTTC | 1 cut(s) | 1508 |
| LmnI | GCTCC | 3 cut(s) | 657, 2217, 2371 |
| Lsp1109I | GCAGC | 1 cut(s) | 1694 |
| LweI | GCATC | 9 cut(s) | 793, 1095, 1199, 1465, 1521, 1618, 1629, 1800, 2331 |
| MaeI | CTAG | 4 cut(s) | 956, 1049, 1799, 2046 |
| MaeII | ACGT | 6 cut(s) | 266, 446, 1135, 1180, 1247, 2260 |
| MaeIII | GTNAC | 6 cut(s) | 92, 413, 560, 605, 2051, 2187 |
| MalI | GATC | 8 cut(s) | 305, 846, 1111, 1119, 1721, 2015, 2147, 2316 |
| MboI | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| MfeI | CAATTG | 1 cut(s) | 1598 |
| MflI | RGATCY | 1 cut(s) | 2145 |
| MhlI | GDGCHC | 5 cut(s) | 654, 1014, 1703, 1823, 2376 |
| MlsI | TGGCCA | 2 cut(s) | 5, 919 |
| MluNI | TGGCCA | 2 cut(s) | 5, 919 |
| MlyI | GAGTC | 3 cut(s) | 450, 1632, 1939 |
| MmeI | TCCRAC | 1 cut(s) | 2128 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 919 |
| Mph1103I | ATGCAT | 3 cut(s) | 808, 1088, 2346 |
| MscI | TGGCCA | 2 cut(s) | 5, 919 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2225 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 919 |
| MspA1I | CMGCKG | 1 cut(s) | 2075 |
| MspCI | CTTAAG | 2 cut(s) | 565, 1838 |
| MspI | CCGG | 3 cut(s) | 713, 1214, 2027 |
| MspR9I | CCNGG | 3 cut(s) | 1214, 1617, 2060 |
| MssI | GTTTAAAC | 1 cut(s) | 1252 |
| MunI | CAATTG | 1 cut(s) | 1598 |
| Mva1269I | GAATGC | 2 cut(s) | 806, 1273 |
| MvaI | CCWGG | 2 cut(s) | 1617, 2060 |
| MvnI | CGCG | 1 cut(s) | 288 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 11, 232, 285, 379, 1092, 1698, 1740 |
| NciI | CCSGG | 1 cut(s) | 1214 |
| NcoI | CCATGG | 1 cut(s) | 1659 |
| NdeII | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| NlaIV | GGNNCC | 5 cut(s) | 943, 1012, 1744, 1820, 2363 |
| NmeAIII | GCCGAG | 5 cut(s) | 269, 516, 1622, 1682, 1943 |
| NmuCI | GTSAC | 3 cut(s) | 413, 2051, 2187 |
| NsbI | TGCGCA | 1 cut(s) | 763 |
| NsiI | ATGCAT | 3 cut(s) | 808, 1088, 2346 |
| NspI | RCATGY | 4 cut(s) | 239, 335, 2120, 2328 |
| PaeI | GCATGC | 1 cut(s) | 239 |
| PagI | TCATGA | 2 cut(s) | 1455, 2209 |
| PciI | ACATGT | 3 cut(s) | 331, 2116, 2324 |
| PciSI | GCTCTTC | 1 cut(s) | 1508 |
| PctI | GAATGC | 2 cut(s) | 806, 1273 |
| PfeI | GAWTC | 5 cut(s) | 393, 924, 1220, 1768, 1828 |
| Pfl23II | CGTACG | 1 cut(s) | 1113 |
| PkrI | GCNGC | 1 cut(s) | 1684 |
| Ple19I | CGATCG | 2 cut(s) | 847, 1112 |
| PleI | GAGTC | 3 cut(s) | 450, 1632, 1938 |
| PmeI | GTTTAAAC | 1 cut(s) | 1252 |
| PpsI | GAGTC | 3 cut(s) | 450, 1632, 1938 |
| Ppu21I | YACGTR | 1 cut(s) | 1181 |
| PpuMI | RGGWCCY | 1 cut(s) | 941 |
| PscI | ACATGT | 3 cut(s) | 331, 2116, 2324 |
| PshAI | GACNNNNGTC | 1 cut(s) | 316 |
| Psp1406I | AACGTT | 1 cut(s) | 1247 |
| Psp5II | RGGWCCY | 1 cut(s) | 941 |
| Psp6I | CCWGG | 2 cut(s) | 1615, 2058 |
| PspEI | GGTNACC | 1 cut(s) | 605 |
| PspGI | CCWGG | 2 cut(s) | 1615, 2058 |
| PspLI | CGTACG | 1 cut(s) | 1113 |
| PspN4I | GGNNCC | 5 cut(s) | 943, 1012, 1744, 1820, 2363 |
| PspOMI | GGGCCC | 1 cut(s) | 1010 |
| PspPI | GGNCC | 6 cut(s) | 941, 1010, 1011, 1733, 1742, 2362 |
| PspPPI | RGGWCCY | 1 cut(s) | 941 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 499, 531 |
| PsuI | RGATCY | 1 cut(s) | 2145 |
| PvuI | CGATCG | 2 cut(s) | 847, 1112 |
| RsaI | GTAC | 6 cut(s) | 480, 954, 1115, 1482, 1973, 1993 |
| RsaNI | GTAC | 6 cut(s) | 479, 953, 1114, 1481, 1972, 1992 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2225 |
| SapI | GCTCTTC | 1 cut(s) | 1508 |
| SatI | GCNGC | 1 cut(s) | 1683 |
| Sau3AI | GATC | 8 cut(s) | 303, 844, 1109, 1117, 1719, 2013, 2145, 2314 |
| Sau96I | GGNCC | 6 cut(s) | 941, 1010, 1011, 1733, 1742, 2362 |
| ScaI | AGTACT | 1 cut(s) | 480 |
| SchI | GAGTC | 3 cut(s) | 450, 1632, 1939 |
| ScrFI | CCNGG | 3 cut(s) | 1214, 1617, 2060 |
| SduI | GDGCHC | 5 cut(s) | 654, 1014, 1703, 1823, 2376 |
| SfaNI | GCATC | 9 cut(s) | 793, 1095, 1199, 1465, 1521, 1618, 1629, 1800, 2331 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1740 |
| SinI | GGWCC | 1 cut(s) | 941 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2225 |
| SmlI | CTYRAG | 3 cut(s) | 352, 565, 1838 |
| SmoI | CTYRAG | 3 cut(s) | 352, 565, 1838 |
| SphI | GCATGC | 1 cut(s) | 239 |
| SsiI | CCGC | 3 cut(s) | 12, 1045, 2075 |
| SspMI | CTAG | 4 cut(s) | 956, 1049, 1799, 2046 |
| StyD4I | CCNGG | 3 cut(s) | 1212, 1615, 2058 |
| StyI | CCWWGG | 3 cut(s) | 655, 1571, 1659 |
| TaaI | ACNGT | 4 cut(s) | 64, 318, 419, 727 |
| TaiI | ACGT | 6 cut(s) | 269, 449, 1138, 1183, 1250, 2263 |
| TaqII | GACCGA | 1 cut(s) | 401 |
| TatI | WGTACW | 1 cut(s) | 478 |
| TfiI | GAWTC | 5 cut(s) | 393, 924, 1220, 1768, 1828 |
| TscAI | CASTG | 2 cut(s) | 632, 2046 |
| TseFI | GTSAC | 3 cut(s) | 413, 2051, 2187 |
| TseI | GCWGC | 1 cut(s) | 1682 |
| Tsp45I | GTSAC | 3 cut(s) | 413, 2051, 2187 |
| TspDTI | ATGAA | 6 cut(s) | 1116, 1366, 1382, 1664, 1869, 2215 |
| TspGWI | ACGGA | 2 cut(s) | 484, 1951 |
| TspRI | CASTG | 2 cut(s) | 632, 2046 |
| Vha464I | CTTAAG | 2 cut(s) | 565, 1838 |
| VpaK11BI | GGWCC | 1 cut(s) | 941 |
| XapI | RAATTY | 4 cut(s) | 86, 1065, 1123, 1285 |
| XceI | RCATGY | 4 cut(s) | 239, 335, 2120, 2328 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1004 |
| XmiI | GTMKAC | 2 cut(s) | 1933, 2121 |
| XspI | CTAG | 4 cut(s) | 956, 1049, 1799, 2046 |
| ZrmI | AGTACT | 1 cut(s) | 480 |
| Zsp2I | ATGCAT | 3 cut(s) | 808, 1088, 2346 |