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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 576, 613 |
| AciI | CCGC | 2 cut(s) | 312, 401 |
| AclWI | GGATC | 1 cut(s) | 426 |
| AcoI | YGGCCR | 2 cut(s) | 466, 607 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 172, 475 |
| AgsI | TTSAA | 5 cut(s) | 149, 475, 487, 539, 620 |
| AluBI | AGCT | 4 cut(s) | 152, 261, 326, 340 |
| AluI | AGCT | 4 cut(s) | 152, 261, 326, 340 |
| Alw26I | GTCTC | 2 cut(s) | 107, 245 |
| AlwI | GGATC | 1 cut(s) | 426 |
| AoxI | GGCC | 2 cut(s) | 466, 607 |
| ApeKI | GCWGC | 1 cut(s) | 104 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 194, 226 |
| AspLEI | GCGC | 1 cut(s) | 505 |
| AsuHPI | GGTGA | 2 cut(s) | 140, 334 |
| AsuNHI | GCTAGC | 1 cut(s) | 563 |
| BbsI | GAAGAC | 1 cut(s) | 138 |
| BbvI | GCAGC | 1 cut(s) | 116 |
| BceAI | ACGGC | 3 cut(s) | 453, 481, 594 |
| BcoDI | GTCTC | 2 cut(s) | 107, 245 |
| BfaI | CTAG | 3 cut(s) | 57, 332, 564 |
| BisI | GCNGC | 2 cut(s) | 105, 402 |
| BlsI | GCNGC | 2 cut(s) | 106, 403 |
| BmsI | GCATC | 1 cut(s) | 550 |
| BmtI | GCTAGC | 1 cut(s) | 567 |
| BpiI | GAAGAC | 1 cut(s) | 138 |
| Bpu10I | CCTNAGC | 1 cut(s) | 322 |
| BpuEI | CTTGAG | 1 cut(s) | 569 |
| BsaJI | CCNNGG | 1 cut(s) | 171 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 624 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 172, 475 |
| Bse1I | ACTGG | 1 cut(s) | 610 |
| BseDI | CCNNGG | 1 cut(s) | 171 |
| BseGI | GGATG | 2 cut(s) | 181, 565 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 172, 475 |
| BseNI | ACTGG | 1 cut(s) | 610 |
| BseRI | GAGGAG | 2 cut(s) | 263, 494 |
| BseX3I | CGGCCG | 1 cut(s) | 466 |
| BseXI | GCAGC | 1 cut(s) | 116 |
| Bsh1285I | CGRYCG | 1 cut(s) | 469 |
| BshFI | GGCC | 2 cut(s) | 468, 609 |
| BsiEI | CGRYCG | 1 cut(s) | 469 |
| BslFI | GGGAC | 2 cut(s) | 389, 614 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 172, 475 |
| BsmAI | GTCTC | 2 cut(s) | 107, 245 |
| BsmFI | GGGAC | 2 cut(s) | 389, 614 |
| BsmI | GAATGC | 1 cut(s) | 567 |
| BsnI | GGCC | 2 cut(s) | 468, 609 |
| Bsp143I | GATC | 1 cut(s) | 418 |
| BspACI | CCGC | 2 cut(s) | 312, 401 |
| BspANI | GGCC | 2 cut(s) | 468, 609 |
| BspHI | TCATGA | 2 cut(s) | 220, 517 |
| BspOI | GCTAGC | 1 cut(s) | 567 |
| BspPI | GGATC | 1 cut(s) | 426 |
| BsrI | ACTGG | 1 cut(s) | 610 |
| BssECI | CCNNGG | 1 cut(s) | 171 |
| BssMI | GATC | 1 cut(s) | 418 |
| BssT1I | CCWWGG | 1 cut(s) | 171 |
| Bst4CI | ACNGT | 1 cut(s) | 76 |
| BstC8I | GCNNGC | 1 cut(s) | 565 |
| BstDEI | CTNAG | 2 cut(s) | 322, 405 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 170 |
| BstF5I | GGATG | 2 cut(s) | 181, 565 |
| BstHHI | GCGC | 1 cut(s) | 505 |
| BstKTI | GATC | 1 cut(s) | 421 |
| BstMAI | GTCTC | 2 cut(s) | 107, 245 |
| BstMBI | GATC | 1 cut(s) | 418 |
| BstMCI | CGRYCG | 1 cut(s) | 469 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 337, 401 |
| BstV1I | GCAGC | 1 cut(s) | 116 |
| BstV2I | GAAGAC | 1 cut(s) | 138 |
| BstX2I | RGATCY | 1 cut(s) | 418 |
| BstYI | RGATCY | 1 cut(s) | 418 |
| BstZI | CGGCCG | 1 cut(s) | 466 |
| BsuRI | GGCC | 2 cut(s) | 468, 609 |
| BtsCI | GGATG | 2 cut(s) | 181, 565 |
| BtsI | GCAGTG | 1 cut(s) | 582 |
| BtsIMutI | CAGTG | 2 cut(s) | 582, 603 |
| Cac8I | GCNNGC | 1 cut(s) | 565 |
| CciI | TCATGA | 2 cut(s) | 220, 517 |
| CfoI | GCGC | 1 cut(s) | 505 |
| CviAII | CATG | 3 cut(s) | 221, 362, 518 |
| CviJI | RGCY | 9 cut(s) | 107, 152, 226, 261, 326, 340, 404, 468, 609 |
| CviKI_1 | RGCY | 9 cut(s) | 107, 152, 226, 261, 326, 340, 404, 468, 609 |
| DdeI | CTNAG | 2 cut(s) | 322, 405 |
| DpnI | GATC | 1 cut(s) | 420 |
| DpnII | GATC | 1 cut(s) | 418 |
| EaeI | YGGCCR | 2 cut(s) | 466, 607 |
| EagI | CGGCCG | 1 cut(s) | 466 |
| EclXI | CGGCCG | 1 cut(s) | 466 |
| Eco130I | CCWWGG | 1 cut(s) | 171 |
| Eco52I | CGGCCG | 1 cut(s) | 466 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 170 |
| EcoT14I | CCWWGG | 1 cut(s) | 171 |
| ErhI | CCWWGG | 1 cut(s) | 171 |
| FaeI | CATG | 3 cut(s) | 224, 365, 521 |
| FaiI | YATR | 9 cut(s) | 140, 156, 214, 222, 363, 429, 519, 557, 630 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 531, 563 |
| FaqI | GGGAC | 2 cut(s) | 389, 614 |
| FatI | CATG | 3 cut(s) | 220, 361, 517 |
| FblI | GTMKAC | 2 cut(s) | 576, 613 |
| Fnu4HI | GCNGC | 2 cut(s) | 105, 402 |
| FokI | GGATG | 2 cut(s) | 188, 572 |
| Fsp4HI | GCNGC | 2 cut(s) | 105, 402 |
| FspBI | CTAG | 3 cut(s) | 57, 332, 564 |
| GlaI | GCGC | 1 cut(s) | 504 |
| GluI | GCNGC | 2 cut(s) | 105, 402 |
| HaeIII | GGCC | 2 cut(s) | 468, 609 |
| HhaI | GCGC | 1 cut(s) | 505 |
| Hin1II | CATG | 3 cut(s) | 224, 365, 521 |
| Hin6I | GCGC | 1 cut(s) | 503 |
| HinP1I | GCGC | 1 cut(s) | 503 |
| HindIII | AAGCTT | 2 cut(s) | 150, 259 |
| HinfI | GANTC | 1 cut(s) | 161 |
| HphI | GGTGA | 2 cut(s) | 140, 334 |
| Hpy166II | GTNNAC | 2 cut(s) | 577, 614 |
| Hpy188III | TCNNGA | 3 cut(s) | 221, 238, 518 |
| Hpy8I | GTNNAC | 2 cut(s) | 577, 614 |
| HpyAV | CCTTC | 2 cut(s) | 57, 237 |
| HpyCH4III | ACNGT | 1 cut(s) | 76 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 337, 401 |
| HpyF3I | CTNAG | 2 cut(s) | 322, 405 |
| Hsp92II | CATG | 3 cut(s) | 224, 365, 521 |
| HspAI | GCGC | 1 cut(s) | 503 |
| Kzo9I | GATC | 1 cut(s) | 418 |
| LpnPI | CCDG | 3 cut(s) | 269, 585, 591 |
| Lsp1109I | GCAGC | 1 cut(s) | 116 |
| LweI | GCATC | 1 cut(s) | 550 |
| MaeI | CTAG | 3 cut(s) | 57, 332, 564 |
| MalI | GATC | 1 cut(s) | 420 |
| MboI | GATC | 1 cut(s) | 418 |
| MboII | GAAGA | 5 cut(s) | 16, 143, 222, 296, 551 |
| MfeI | CAATTG | 1 cut(s) | 144 |
| MflI | RGATCY | 1 cut(s) | 418 |
| MluCI | AATT | 5 cut(s) | 21, 80, 144, 534, 625 |
| MmeI | TCCRAC | 3 cut(s) | 75, 289, 317 |
| MnlI | CCTC | 8 cut(s) | 98, 119, 175, 241, 366, 409, 470, 472 |
| MseI | TTAA | 1 cut(s) | 591 |
| MunI | CAATTG | 1 cut(s) | 144 |
| Mva1269I | GAATGC | 1 cut(s) | 567 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 337, 401 |
| NdeII | GATC | 1 cut(s) | 418 |
| NheI | GCTAGC | 1 cut(s) | 563 |
| NlaIII | CATG | 3 cut(s) | 224, 365, 521 |
| PagI | TCATGA | 2 cut(s) | 220, 517 |
| PctI | GAATGC | 1 cut(s) | 567 |
| PfeI | GAWTC | 1 cut(s) | 161 |
| PkrI | GCNGC | 2 cut(s) | 106, 403 |
| PsuI | RGATCY | 1 cut(s) | 418 |
| SaqAI | TTAA | 1 cut(s) | 591 |
| SatI | GCNGC | 2 cut(s) | 105, 402 |
| Sau3AI | GATC | 1 cut(s) | 418 |
| SetI | ASST | 9 cut(s) | 130, 154, 263, 288, 323, 328, 342, 462, 549 |
| SfaNI | GCATC | 1 cut(s) | 550 |
| SmlI | CTYRAG | 1 cut(s) | 548 |
| SmoI | CTYRAG | 1 cut(s) | 548 |
| Sse9I | AATT | 5 cut(s) | 21, 80, 144, 534, 625 |
| SsiI | CCGC | 2 cut(s) | 312, 401 |
| SspMI | CTAG | 3 cut(s) | 57, 332, 564 |
| StyI | CCWWGG | 1 cut(s) | 171 |
| TaaI | ACNGT | 1 cut(s) | 76 |
| TaqI | TCGA | 2 cut(s) | 90, 237 |
| TasI | AATT | 5 cut(s) | 21, 80, 144, 534, 625 |
| TauI | GCSGC | 1 cut(s) | 404 |
| TfiI | GAWTC | 1 cut(s) | 161 |
| Tru1I | TTAA | 1 cut(s) | 591 |
| Tru9I | TTAA | 1 cut(s) | 591 |
| TscAI | CASTG | 2 cut(s) | 589, 610 |
| TseI | GCWGC | 1 cut(s) | 104 |
| TspDTI | ATGAA | 4 cut(s) | 17, 222, 378, 534 |
| TspRI | CASTG | 2 cut(s) | 589, 610 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 170 |
| XmiI | GTMKAC | 2 cut(s) | 576, 613 |
| XspI | CTAG | 3 cut(s) | 57, 332, 564 |